scholarly journals Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat

2020 ◽  
Vol 12 (2) ◽  
pp. 114-122
Author(s):  
Wahyu Irawati ◽  
Aaron Hasthosaputro ◽  
Lucia Kusumawati

The increasing industrial activity in Indonesia, that is not equipped with appropriate waste treatment, has caused an increase of heavy metal contaminants in water bodies. Heavy metals contamination such as copper (Cu), mercury (Hg), cadmium (Cd), and lead (Pb) contamination in water bodies have endangered aquatic life and public health. For this reason, it is urgently important to lower down the concentration of heavy metal pollutants in the water bodies surrounding industrial areas. Compared to chemical remediation, bioremediation of heavy metal by using indigenous bacteria is more effective and economical, since it can be applied in situ directly and be used repeatedly. Acinetobacter sp. IrC2, used in this study, is Indonesian indigenous bacteria isolated from the industrial waste treatment facility in Rungkut, Surabaya. This study aims, firstly, to investigate the heavy metal multiresistance of Acinetobacter sp. IrC2 against mercury, cadmium, and lead. Secondly, this study intends to examine its bioaccumulation capacity for single and heavy metal alloys. The heavy metal multiresistance test was carried out by measuring the minimum heavy metal concentrations that inhibit bacterial growth (Minimum Inhibitory Concentration/MIC). The bioaccumulation capacity was measured using an atomic absorption spectrophotometer (AAS).  It is shown that Acinetobacter sp. IrC2 has high multiresistance to mercury, cadmium, and lead with MIC values of 12 mM, 8 mM, and 18 mM, respectively. Furthermore,  it is also resistant to  heavy metal mixture of 4.5 mM.  The mechanism of bacterial resistance in response to heavy metal toxicity, in general, is by accumulating heavy metals in the cells. The highest amount of accumulated heavy metals identified, from bacteria grown in the medium contains a mixture of heavy metals, were 0.023 mg, 0.084 mg, 0.684 mg, and 1.476 mg per gram of cell dry weight for copper, mercury, cadmium and lead respectively.  In conclusion, Acinetobacter sp. IrC2 is a promising heavy metal bioremediation agent due to its heavy metal multiresistance and accumulator characteristics.  Key words: Acinetobacter sp. IrC2; cadmium; copper; lead; merkuri

Author(s):  
Khalid Mahmood Anjum ◽  
Atif Yaqub ◽  
Ehsan Mehmood Bhatti ◽  
Muhamad Yameen ◽  
Muhammad Sharif Mughal ◽  
...  

Reasonable evidences suggest that heavy metal pollutants have been added by factories and other industries to our local water bodies. The present study was conducted to determine the heavy metal (zinc and lead) concentrations in muscle tissues of Labeo rohita inhabiting local water bodies as well as those being raised at farms. Sampling was performed to collect Labeo rohita from two different stations along river Ravi, viz. Lahore Siphon (upstream) and Balloki Headworks (downstream). Additionally, samples were also obtained from the government operated as well as private fish farms located in Lahore District, Pakistan. The fish were dissected; their muscles were digested, filtered, and finally analyzed for concentrations of Zn and Pb. Significant variation of heavy metal ions were observed between two sites along river Ravi as well as government operated and private fish farms. Significantly high mean concentrations of Zn (10.7±1.268) and Pb (0.61±0.082) were observed at Balloki Head-works; whereas, the lowest Zn (1.58±0.235) and Pb (0.16±0.008) heavy metal levels were recorded in the muscle samples collected from private fish farms. It was also observed that the heavy metal concentration in the muscles of Labeo rohita, collected from Balloki Head-works was much higher than the permissible limits laid down by WHO/FAO. Further the recorded highest level of heavy metals in water samples collected from River Ravi indicates that the bioaccumulation of heavy metals is severely affecting the aquatic life of fresh water and industries should not be allowed to release their effluent directly into the river Ravi in their vicinity.


2016 ◽  
Vol 11 (1) ◽  
pp. 325-332 ◽  
Author(s):  
Pammi Singh ◽  
Mithra Dey ◽  
Sunkam Narayana

Considering the significance of heavy metal pollution in aquatic system bioaccumulation of heavy metals in two species of tadpoles namely Clinotarsus alticola and Leptobrachium smithicollected from tea gardens of Barak valley, Assam was studied. Aquatic life is affected by heavy metal pollutants present in water as well as in sediment. The result of the study revealed that the concentration of iron, chromium, cadmium and lead in water samples was higher than the permissible limit of 0.3, 0.05, 0.003, 0.01 mg/L respectively but that of copper and zinc concentration was within the maximum permissible limit of 2 mg/L and 3 mg/L (WHO, 2005). The accumulation pattern of different heavy metals in different organs viz., intestine, liver and tail was studied.Overall the metal burden in different organs of Clinotarsus alticola and Leptobrachium smithi was in the order of liver>tail>intestine. Liver had highest accumulation of metals while intestine accumulated the least.Iron (Fe) was highly and zinc (Zn) was the least accumulated metal in both the tadpoles. The accumulation of heavy metals might be due to tea plantation influx water, domestic and associated anthropogenic activities.


2021 ◽  
Vol 43 (5) ◽  
pp. 611-611
Author(s):  
Fehmeeda Afzal Fehmeeda Afzal ◽  
Sonia Tariq Sonia Tariq ◽  
Ashraf Nadeem Ashraf Nadeem ◽  
Samiullah Samiullah ◽  
Jafar Iqbal Jafar Iqbal ◽  
...  

Heavy metals are an important class of compounds that is increasing in environment due to the anthropogenic activities. They are extremely toxic to human beings and animals. Many of them enter the water through industrial waste and effect the aquatic life. Fishes, for example, are an important source of food and they get effected by heavy metals when they ingest the infected sediments, mud and water. Heavy metals enter the food chain when infected fishes are consumed and effect humans as well. We highlight the recent (10-15 years) published work on measuring the levels of heavy metal in Mugilcephalus (Flathead mullet) fish. Out of all the methods compared and discussed in this paper, Atomic absorption spectroscopy was found to be the most preferred method and most commonly used by researchers because of its ease and cost. The most commonly used unit for metal detection in muscles was g/g (dry weight).The results also summarize advantages and drawbacks of methods used to digest muscles of the said species and heavy metal measurement. In selected studies, the levels of metals were also compared with the safe limits set by WHO, FAO and USEPA. This gives a lot of information about the edible fitness of the M.cephalus.


2017 ◽  
Vol 5 (2) ◽  
pp. 252-265
Author(s):  
Esther Isabella Eucharista

Heavy metals are naturally occurring elements that have a high atomic weight than that of water. Their multiple industrial, domestic, agricultural, medical and technological applications have led to their wide distribution in the environment; raising concerns over their potential effects on human health and the environment. The maximum Zn concentration was noticed in the year 2012 as 24.47±0.57µg/l in the water bodies of Tiruchendur and Srivaikundam taluk at station X. The highest Zn concentration was obtained in the water sediments as 272.33±5.01 µg/g at station 1 in the year 2012 and the concentration was high in the phytoplankton as 34.314±4.239 µg/g at station V in the year 2011. During the year 2012, the cu was found high as 22.53±15.70 µg/l at station III in the water bodies. In the sediments it was high as 63.03±13.85 µg/l at station XI during 2011. In the phytoplankton the maximum concentration was noticed at station VIII as 13.564±2.797 µg/g during 2011. During the year 2011 the highest concentration of Pb was emphasized at station VI as 11.31±1.63 µg/l in the water bodies. In the water sediments it was high at station V as 64.11±11.96 µg/g during 2011. In the phytoplankton, the concentration was measured highest at station II as 4.343±0.976 µg/g dry weight in the year 2012. All the heavy metals such as Zn, Cu and Pb were exceeded the Shale reference background value. This review provides an analysis of their environmental occurrence and potential for human exposure.


2011 ◽  
Vol 138-139 ◽  
pp. 1149-1155 ◽  
Author(s):  
Yi Dong Guan ◽  
Ye Hong Du ◽  
Zhen Dong Li ◽  
An Cheng Luo

This paper reports the concentration of heavy metals (Cr, Cu, Zn, Cd and Pb) in the soils and rices surrounding the abandoned rural waste dumping sites in Ningbo. Igeo (geoaccumulation index) was calculated to assess the contamination degree of heavy metals in soils. The mean contents of Cr, Cu, Cd, Zn and Pb of soils were 33.3, 24.1, 1.5, 118.9 and 45.6 mg/(kg DW) (dry weight), respectively. All of them were much higher than that of the reference value (i.e. CK), but there were no coherent trend of the metal contents within 1-120m distance from the dumping site. Igeo of heavy metals reveals the order of Cd>Cu>Cr>Pb>Zn, and the contamination assessment of soils using Igeo indicate the moderate Cd pollution, while the soils were unpolluted-moderately overall by Cr, Cu, Zn as well as Pb. The heavy metal contents in root, stem & leaf and rice grains were all remarkable higher than that of the CK at 20-120 m distances, and the heavy metal contents in root were evidently much higher than other plant parts, while those in rice grain were lowest, indicating the great bioaccumulation trend of heavy metals. Although the metal contents in the rice grain were within the legislation limit, its bioaccumulation trend of heavy metals was remarkable, whose contents were 4.38-fold for Cr, 1.76-fold for Cu, 1.28-fold for Zn, 2.67-fold for Cd and 3.03-fold for Pb higher than that of reference value, respectively. Finally, we proposed a decentralized in-situ restoration approach for the dumping sites.


2020 ◽  
Vol 36 (6) ◽  
pp. 1154-1160
Author(s):  
G. DEEPA ◽  
M. JEYARAJ ◽  
P. N. Magudeswaran

On account of industrialization and increasing population, the water bodies get polluted by means of degradable and non-degradable substances. In 21st century, it is necessary to maintain a healthy environment especially water bodies for the survival of not the aquatic animals but also for healthy human life. Recent advances suggest that the issues related to water quality could be resolved by using nanoparticles and nano-filtration membrane methods from the development of nanotechnology. In this research, attempt to remove heavy metals from Chithrapuzha River water at Cochin bar mouth (S1) and Fact barge jetty (S2) using Fe2O3 prepared via green synthesis using Egg albumin and Aloe vera. Our results provoke that, the synthesis of Fe2O3 nanoparticle is cost-effective and eco-friendly and also good in nano-regime. Results of filtration studies showed that Fe2O3 nanoparticles remove heavy metals from Chithrapuzha River water and also increases the DO content which helps the survival of aquatic life.


Author(s):  
Tiejun Wang ◽  
Xiaoyu Wang ◽  
Wei Tian ◽  
Lunguang Yao ◽  
Yadong Li ◽  
...  

Microbial immobilization is considered as a novel and environmentally friendly technology that uses microbes to reduce heavy metals accumulation in plants. To explore microbial resources which are useful in these applications, three water spinach rhizosphere soils polluted by different levels of heavy metals (heavy pollution (CQ), medium pollution (JZ), and relative clean (NF)) were collected. The community composition of heavy metal-immobilizing bacteria in rhizosphere soils and its effects on reducing the Cd2+ and Pb2+ concentrations in water spinach were evaluated. Four hundred strains were isolated from the CQ (belonging to 3 phyla and 14 genera), JZ (belonging to 4 phyla and 25 genera) and NF (belonged to 6 phyla and 34 genera) samples, respectively. In the CQ sample, 137 strains showed a strong ability to immobilize Cd2+ and Pb2+, giving Cd2+ and Pb2+ removal rates of greater than 80% in solution; Brevundimonas, Serratia, and Pseudoarthrobacter were the main genera. In total, 62 strains showed a strong ability to immobilize Cd2+ and Pb2+ in the JZ sample and Bacillus and Serratia were the main genera. A total of 22 strains showed a strong ability to immobilize Cd2+ and Pb2+ in the NF sample, and Bacillus was the main genus. Compared to the control, Enterobacter bugandensis CQ-7, Bacillus thuringensis CQ-33, and Klebsiella michiganensis CQ-169 significantly increased the dry weight (17.16–148%) of water spinach and reduced the contents of Cd2+ (59.78–72.41%) and Pb2+ (43.36–74.21%) in water spinach. Moreover, the soluble protein and Vc contents in the shoots of water spinach were also significantly increased (72.1–193%) in the presence of strains CQ-7, CQ-33 and CQ-169 compared to the control. In addition, the contents of Cd and Pb in the shoots of water spinach meet the standard for limit of Cd2+ and Pb2+ in vegetables in the presence of strains CQ-7, CQ-33 and CQ-169. Thus, the results provide strains as resources and a theoretical basis for the remediation of Cd- and Pb-contaminated farmlands for the safe production of vegetables.


2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ismael Acosta-Rodríguez ◽  
Juan F. Cárdenas-González ◽  
Adriana S. Rodríguez Pérez ◽  
Juana Tovar Oviedo ◽  
Víctor M. Martínez-Juárez

The objective of this work was to study the resistance and removal capacity of heavy metals by the fungusAspergillus niger. We analyzed the resistance to some heavy metals by dry weight and plate: the fungus grew in 2000 ppm of zinc, lead, and mercury, 1200 and 1000 ppm of arsenic (III) and (VI), 800 ppm of fluor and cobalt, and least in cadmium (400 ppm). With respect to their potential of removal of heavy metals, this removal was achieved for zinc (100%), mercury (83.2%), fluor (83%), cobalt (71.4%), fairly silver (48%), and copper (37%). The ideal conditions for the removal of 100 mg/L of the heavy metals were 28°C, pH between 4.0 and 5.5, 100 ppm of heavy metal, and 1 g of fungal biomass.


2019 ◽  
Vol 7 (12) ◽  
pp. 697 ◽  
Author(s):  
Chaolin Fang ◽  
Varenyam Achal

The global energy crisis and heavy metal pollution are the common problems of the world. It is noted that the microbial fuel cell (MFC) has been developed as a promising technique for sustainable energy production and simultaneously coupled with the remediation of heavy metals from water and soil. This paper reviewed the performances of MFCs for heavy metal removal from soil and water. Electrochemical and microbial biocatalytic reactions synergistically resulted in power generation and the high removal efficiencies of several heavy metals in wastewater, such as copper, hexavalent chromium, mercury, silver, thallium. The coupling system of MFCs and microbial electrolysis cells (MECs) successfully reduced cadmium and lead without external energy input. Moreover, the effects of pH and electrode materials on the MFCs in water were discussed. In addition, the remediation of heavy metal-contaminated soil by MFCs were summarized, noting that plant-MFC performed very well in the heavy metal removal.


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