scholarly journals Investigation of antioxidant activity of the hydrolysate derived from Tra catfish byproducts using Alcalase® 2.4 L FG for application as a natural antioxidant ingredient

2016 ◽  
Vol 19 (3) ◽  
pp. 110-122 ◽  
Author(s):  
Tam Dinh Le Vo ◽  
Thao Thi Huong Nguyen ◽  
Du Van Phan ◽  
Huy Do Minh Nguyen ◽  
Huy Quang Tran
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
G Protein ◽  
Butylated Hydroxytoluene ◽  
Natural Antioxidant ◽  
Antioxidant Potential ◽  
Hydrolysis Time ◽  
Enzyme Substrate ◽  
Tra Catfish ◽  
By Products

In this study, the effects of temperature, pH, enzyme content, hydrolysis time on antioxidant activity of the hydrolysate from Tra catfish (Pangasiushypophthalmus) by-products with Alcalase® 2.4 L FG were investigated using DPPH• (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method (DPPH• SM) and FRAP (ferric reducing antioxidant potential) method. The chemical composition of the Tra catfish byproducts included 58.5% moisture, 33.88% crude protein, 50.14% crude lipid and 15.83% ash (on dry weight basis). The result of antioxidant activity of the hydrolysate showed that the 50% DPPH• inhibition concentration (IC50) of the hydrolysate reached about 6775 μg/mL which was 1645-fold higher than that of vitamin C and 17-fold higher than that of BHT (Butylated Hydroxytoluene) with the degree of hydrolysis (DH) of the hydrolysate of 14.6% when hydrolysis time was 5h, enzyme/substrate (E/S) ratio was 30 U/g protein, hydrolysis temperature was 550C, and pH was 7.5. The antioxidant potential of hydrolysate using FRAP method reached about 52.12 μMTrolox equivalent which was 53-fold and 18-fold lower than those of vitamin C and BHT, respectively, when the hydrolysis time was 5h, enzyme/substrate ratio was 30 U/g protein, temperature was 500C, and pH level was 8. The result showed that the antioxidant proteolysate derived from Tra catfish by-products has the potential to be used as a natural antioxidant ingredient in nutraceutical and functional food industry.

scholarly journals Investigation of antioxidant capacity of peptide fractions from the Tra catfish by-product-derived proteolysate using Flavourzyme® 500 mg

2018 ◽  
Vol 20 (K7) ◽  
pp. 35-40
Author(s):  
Tam-Dinh Le-Vo ◽  
Cuong Vi Tran
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Potential ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Crude Lipid ◽  
Trolox Equivalent ◽  
Tra Catfish ◽  
Peptide Fractions ◽  
By Products

In this study, the antioxidant potential of peptide fractions isolated from the Tra catfish (Pangasius hypophthalmus) by-product-derived hydrolysate using ultrafiltration centrifugal devices with 3 distinct molecular-weight cutoffs (MWCOs) of 5KDa, 10KDa, and 30KDa was investigated. Firstly, the chemical composition of the Tra catfish byproducts was analyzed. The result showed that the Tra catfish by-products contained 58.5% moisture, 33.9% crude protein, 50.1% crude lipid and 15.8% ash (on dry weight basis). Secondly, the effects of enzyme content, hydrolysis time on the antioxidant activity of the hydrolysate were studied using DPPH• (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method (DPPH• SM), and FRAP (ferric reducing antioxidant potential) method. Flavourzyme® 500 MG was used for hydrolysis. The result revealed that the antioxidant activity of the proteolysate reached the peak when the hydrolysis time was 3h, enzyme/substrate (E/S) ratio was 25 U/g protein, hydrolysis temperature was 500C, pH was 7, and the degree of hydrolysis (DH) of the hydrolysate reached 37.4%. Next, the proteolysate was further fractionated using MWCOs of 5KDa, 10KDa, and 30KDa and the peptide fractions were investigated for their antioxidant activity. The result showed that the <5KDa fraction showed strongest antioxidant activity with the 50% DPPH• inhibition concentration (IC50) of 3079.34±75.29 μg/mL and FRAP value of 611.28±4.5 μM Trolox equivalent. The second strongest fraction was 5-10KDa with the IC50 and FRAPS value of 4709.3 ± 222.22 μg/mL and 323.62±2.54 μM Trolox equivalent, respectively. The 10-30KDa fraction showed weakest antioxidant capacity with the IC50 of 5847.73 ± 128.68 μg/mL and FRAP value of 291.4±2.65 μM Trolox equivalent. The antioxidant peptides derived from Tra catfish by-products proteolysate have the potential to be used as natural antioxidant ingredients in pharmaceutical and food industry.

PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

scholarly journals Nutrients and bioactive compounds of pulp, peel and seed from umbu fruit

2019 ◽  
Vol 49 (4) ◽  
Author(s):  
Leilson de Oliveira Ribeiro ◽  
Eliseth de Souza Viana ◽  
Ronoel Luiz de Oliveira Godoy ◽  
Sidinea Cordeiro de Freitas ◽  
Suely Pereira Freitas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
Fresh Pulp ◽  
Main Barrier ◽  
By Products

ABSTRACT: The objective of this research was to evaluate the nutritional composition and bioactive compounds of whole umbu fruit, including pulp, seed and peel, and also of a commercial umbu pulp. Samples of the fractions and of commercial pulp were analyzed for determination of minerals and proximate composition, total phenolic and antioxidant activity. Pulps and peel were also analyzed for vitamin C and carotenoids contents. Commercial pulp presented better nutritional composition than fresh pulp (P<0.05) and the peel presented higher phenolic content and antioxidant activity than seed. Peel also stood out by its vitamin C (79 mg.100 g-1) and total carotenoids (2,751 µg.100 g-1) contents, showing that, as the main barrier of the fruit for its protection, it is a fraction rich in bioactive compounds. The highest dietary fiber and iron contents were observed in umbu seed (P<0.05). Therefore, umbu by-products may be ingredients proper for development of food richer in nutrients and bioactive compounds.

scholarly journals Agro-industrial fruit co-products in Colombia, their sources and potential uses in processed food industries: a review

2015 ◽  
Vol 68 (2) ◽  
pp. 7729-7742 ◽  
Author(s):  
Miguel Ángel Alarcón García ◽  
Jairo Humberto López Vargas ◽  
Diego Alonso Restrepo Molina
Keyword(s):  
Antioxidant Activity ◽  
Environmental Pollution ◽  
Dietary Fiber ◽  
Natural Antioxidant ◽  
Processed Foods ◽  
Processed Food ◽  
Food Industries ◽  
High Interest ◽  
Food Matrices ◽  
By Products

Fruit agribusinesses generate large amounts of by-products with diverse characteristics that are inherent to the fruits from which they come, which are a source of great use potential because their compositions include molecules that are currently of high interest (antioxidants and dietary fiber). It is clear that, without correct handling and disposal, theses fruits present a problem due to the environmental pollution that large quantities of residues can generate. Although there are varied uses for agro-industrial co-products, this review focused on the potential uses that co-products could have in different processed food matrices. In this sense, this paper led to the revelation that one of the principal objectives of the reviewed research was to condition co-products for use in processed foods in an attempt to take advantage of the bio-active compounds they contain, principally the natural antioxidant activity, which especially enjoys acceptance by consumers of processed foods.

scholarly journals POTENSI EKSTRAK ETANOL DAUN ANDONG MERAH (Cordyline fruticosa (L.) A. Cheval) SEBAGAI ANTIOKSIDAN PENANGKAL RADIKAL DPPH

2021 ◽  
Vol 4 (1) ◽  
pp. 24
Author(s):  
Yuri Pratiwi Utami
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Chemical Compounds ◽  
Antioxidant Potential ◽  
Traditional Medicinal Plants ◽  
Ic50 Value ◽  
Cordyline Fruticosa

AbstractAndong leaf is one of the traditional medicinal plants that is proven to have various properties including as a (natural) medicinal ingredient. Andong plants contain several chemical compounds including saponins, tannins, flavonoids, polyphenols, steroids. The purpose of this study was to determine the potential of red andong leaf extract (Cordyline fruticosa (L.) A. Cheval) as a radical scavenging antioxidant for DPPH. In this study red andong leaves (Cordyline fruticosa (L.) A. Cheval) were extracted using maceration method with 70% ethanol as solvent. The results showed that anthocyanins were cyanidin. Antioxidant activity was measured by reducing DPPH free radicals with UV-Vis spectrophotometry at a wavelength of 517 nm. The results showed that red andong red leaf extract had strong antioxidant potential with an IC50 value of 64.5197 g/mL against free radicals DPPH and vitamin C as a comparison showed a very strong antioxidant potential with an IC50 value of 2.12 g/mL.Keywords : Andong leaf, antioxidant, DPPH AbstrakDaun andong merupakan salah satu tanaman obat tradisional yang terbukti memiliki berbagai khasiat diantaranya sebagai bahan obat (alami). Tanaman andong mengandung beberapa senyawa kimia diantaranya yaitu saponin, tanin, flavonoid, polifenol, Steroida. Tujuan dari penelitian ini adalah untuk mengetahui potensi  ekstrak daun andong merah (Cordyline fruticosa (L.) A. Cheval) sebagai antioksidan penangkal  radikal DPPH. Pada penelitian ini daun andong merah (Cordyline fruticosa (L.) A. Cheval) diekstraksi dengan menggunakan metode maserasi dengan pelarut etanol 70%. Hasil penelitian menunjukkan  antosianin jenis  sianidin. Aktivitas antioksidan diukur melalui peredaman radikal bebas DPPH dengan spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil Penelitian menunjukkan ekstrak daun andong merah   memiliki potensi  antioksidan  kuat dengan nilai IC50 64.5197 µg/mL terhadap radikal bebas  DPPH dan vitamin C sebagai pembanding menunjukkan potensi antioksidan sangat kuat dengan nilai  IC50 2.12 µg/mL.Kata Kunci : Daun andong merah, antioksidan, DPPH

scholarly journals Effect of pepsin hydrolysis on antioxidant activity of jellyfish protein hydrolysate

2021 ◽  
Vol 302 ◽  
pp. 02010
Author(s):  
Pratchaya Muangrod ◽  
Wiriya Charoenchokpanich ◽  
Vilai Rungsardthong ◽  
Savitri Vatanyoopaisarn ◽  
Benjamaporn Wonganu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protein Hydrolysate ◽  
Inhibitory Effect ◽  
Raw Material ◽  
Degree Of Hydrolysis ◽  
Dpph Radical ◽  
Radical Scavengers ◽  
Hydrolysis Time ◽  
Hydrolysis Of ◽  
By Products

Edible jellyfish have been consumed as food for more than a century with offering high protein and crunchy texture. The pepsin hydrolysis of jellyfish protein yields jellyfish protein hydrolysate (ep-JPH), reported for potential bioactivities such as antioxidant activity or antihypertensive activities. Due to the substantial number of by-products generated from jellyfish processing, the by-products were then selected as a raw material of JPH production. This research aimed to evaluate the effect of the hydrolysis time of pepsin on the antioxidant activity of ep-JPH. The dried desalted jellyfish by-products powder was enzymatically hydrolysed by 5% (w/w) pepsin, and the hydrolysis time was varied from 6, 12, 18, and 24 h at 37oC. Results showed that increased hydrolysis time increased the degree of hydrolysis (DH) and inhibition of DPPH radical. The 24 h ep-JPH possessed the highest DH and the highest inhibitory effect of DPPH radical. The results demonstrated that, in this experiment, all ep-JPHs were DPPH radical scavengers, exhibiting different inhibition activities depending on DH values.

scholarly journals Antioxidant Activity of Pandanus amaryllifolius Leaf and Root Extract and its Application in Topical Emulsion

2016 ◽  
Vol 12 (3) ◽  
pp. 425-431 ◽  
Author(s):  
Ampa Jimtaisong ◽  
Panvipa Krisdaphong
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Vitamin C ◽  
Propylene Glycol ◽  
Leaf Extract ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Oil In Water ◽  
Dpph Activity ◽  
Pandanus Amaryllifolius

Purpose: To develop Thai pandan (Pandanus amaryllifolius) as an antioxidant ingredient for topical emulsion.Methods: Dried leaf and root of P. amaryllifolius (Pandanceae) were extracted separately by maceration with either ethanol or propylene glycol. Antioxidant capacity was investigated by 2,2-diphenyl-1-picry hydrazyl radical (DPPH) and linoleic acid peroxidation method. The total phenoliccontent was measured by Folin-Ciocalteau assay. Oil-in-water topical emulsions containing the individual extracts were prepared and tested for stability.Results: Propylene glycol extract exhibited higher DPPH activity and total phenolic content than the ethanol extract while the DPPH activity of the leaf extract was higher than that of the root. The 50 % inhibition concentration (IC50) value of leaf and root extracts was 0.810 and 2.340 mg/ml, respectively. Although the antioxidant activity of the crude extracts was lower than that of standard vitamin C and butylated hydroxytoluene (BHT), the ethanol/propylene glycol solution extract (ethanol was reduced to50 % extract volume) showed higher inhibition (90.1 %) of linoleic acid peroxidation than 200 ppm of vitamin C (57.1 %) and BHT (71.1 %). An oil-in-water emulsion containing 3 % of the ethanol and propylene glycol extract showed creamy texture with medium viscosity and demonstrated good stability under accelerated aging test.Conclusion: The results indicate a potential for the development of P. amaryllifolius leaf extract as an antioxidant ingredient in topically applied formulations.Keywords: Pandanus amaryllifolius, Extraction, Antioxidant, Emulsion, Stability.

scholarly journals Recovery of bone powder from salmon by- product and application in production of Tra catfish cake

2019 ◽  
Vol 20 (K9) ◽  
pp. 38-44
Author(s):  
Tam Dinh-Le Vo ◽  
Chi Bao Vo ◽  
Huynh Thi Thuy Nguyen ◽  
Linh Truc Le ◽  
Do Thong Trong Vi ◽  
...  
Keyword(s):  
Ph Value ◽  
Hydrolytic Enzyme ◽  
Dry Weight ◽  
Gel Strength ◽  
Ash Content ◽  
Mineral Supplement ◽  
Enzyme Substrate ◽  
Bone Powder ◽  
Tra Catfish ◽  
By Products

In this study, salmon by-products were utilised to produce high ash content (AC) salmon bone powder (SBP) using hydrolytic enzyme and apply in the production of Tra catfish cake. The by- products included 61.9% of moisture, 43.1% of protein, 45.4% of lipid, 10.2% of ash, 3.7% of calcium and 2.3% of phosphorus (on dry weight basis). The AC of SBP achieved the maximal value of 46.9% when hydrolytic enzyme of Alcalase, pH value of 6.5, temperature of 60oC, the Enzyme/Substrate (E/S) ratio of 60 U/g protein and time of 4 hours. The obtained SBP contained 23.1% of calcium, 11.7% of phosphorus and without heavy metals such as cadmium, mercury and lead. The fish cake added with 2% of SBP owned the highest gel strength, folding score and whiteness of 287.4, AA and 65.3, respectively and it was accepted by the test panel. Hence, SBP could be used in the production of Tra catfish cake as a mineral supplement.

scholarly journals Antioxidant Activity from Various Tomato Processing

2016 ◽  
Vol 8 (1) ◽  
pp. 127 ◽  
Author(s):  
Retno Sri Iswari ◽  
R Susanti
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Experimental Method ◽  
Chemical Compound ◽  
Antioxidant Activities ◽  
Antioxidant Potential ◽  
Research Results ◽  
Tomato Processing ◽  
Nutrition Intake ◽  
Β Carotene

Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the body's nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, β-carotene, vitamin C, α-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. α-tocopherol, β-carotene and vitamin C) and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.

scholarly journals Selecting optimal parameters for obtaining the extract of red grape pomace

2022 ◽  
Vol 75 (1) ◽  
Author(s):  
Lela Gurgenidze ◽  
Tamar Kanchaveli ◽  
Giorgi Kvartskhava
Keyword(s):  
Antioxidant Activity ◽  
Food Industry ◽  
Activity Level ◽  
Natural Antioxidant ◽  
Extraction Temperature ◽  
Optimal Parameters ◽  
Water Solvent ◽  
Physical Chemical ◽  
Antioxidant Characteristics ◽  
By Products

Due to the industrial processing of grapes, large amounts of by-products are produced. The main varieties of by-products are pomace, which is comprised of skins, seeds and any other solid remaining after pressing process and sediments. It is necessary to implement new effective ways of processing to minimize these residues. This problem is relevant for all the wine-producing countries, including Georgia. It is well-known fact that pomace is an important source of phenolic compounds, which are characterized by high antioxidant activity and possess healing-prophylactic properties. It is also worth mentioning that pomace is an easily spoiled product, and without the proper processing, it cannot be stored for a long time. Thus, this research aimed to obtain optimal parameters for extraction, preserving the antioxidant characteristics. The optimal range of the following parameters for extraction was determined: the temperature for drying 45-50 °C, grinding level 1.5 mm, diluent concentration 70% ethanol/water solvent, extraction module 1:20, extraction temperature 50-55 °C, and duration 2 h. This determination of technological parameters of extraction was done according to the best physical-chemical measures and antioxidant activity level. The physical-chemical tests were performed according to the European Union standards. These parameters can produce an extract with distinct antioxidant characteristics that can be used in the food industry as a natural antioxidant. The extract with distinct antioxidant properties was obtained, which can be used in the food industry as a natural antioxidant.

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