scholarly journals Effectiveness of Extracts of Garlic (Allium sativum L) and Red Ginger (Zingiber officinale var rubra) as Escherichia coli Control in Broiler Chicken

2018 ◽  
Vol 5 (3) ◽  
Author(s):  
R N Damanik ◽  
M Tafsin ◽  
A H Daulay ◽  
E Mirwandhono ◽  
T H Wahyuni

This study aims to determine the effectiveness of extracts of garlic (Allium sativum L.) and ginger (Zingiber officinale) on the growth of broiler chickens infected with E. coli. The design used in this study was a Completely Randomized Design with 6 treatments and 3 replications. The treatment consisted of P0A = control without infection, P0B = control + E. coli infection, P1 = E. coli infection + 0.05% tetracycline antibiotics, P2 = E. coli infection + 1% garlic (Allium sativum L.) extract , P3 = E. coli infection + 1% ginger (Zingiber officinale) extract and P4 = E. coli infection + garlic (Allium sativum L) extract and 1% ginger (Zingiber officinale). The results showed that the administration of garlic and ginger extract had a significant effect (P <0.05) on the decrease in the number of Escherichia coli bacteria in broiler chicken feces. The combination of extracts of garlic (Allium sativum L.) and red ginger (Zingiber officinale) has the most effective effect compared to garlic and ginger extract alone. It was concluded that the administration of extracts of garlic and ginger could be used as Escherichia coli control in broiler chickens.

2018 ◽  
Vol 5 (3) ◽  
Author(s):  
A W Isma ◽  
M Tafsin ◽  
Y L Henuk ◽  
N D Hanafi ◽  
Hamdan

This study aims to examine the effect of garlic (Allium sativum Linn) and red ginger (Zingiber officinale var rubra) extract on the growth of broiler chickens were infected by Escherichia coli. The design used in this study was a complete randomized design (CRD) with 6 treatments and 3 replications. Treatment consists of P0A = Control without infection; P0B = Control + E. coli infection; P1 = Infection E. coli + tetracycline antibiotics 0.05%; P2 = Infection E. coli + garlic extract (Allium sativum Linn) (1%); P3 = Infection E. coli + red ginger extract (Zingiber officinale var rubra) (1%); P4 = Infection E. coli + garlic (Allium sativum Linn) and red ginger (Zingiber officinale var rubra) extract (1%). The results showed that the effect of garlic and red ginger gave significant influence (P <0,05) to growth of broiler were infected by Escherichia coli. The combination of garlic (Allium sativum Linn) and red ginger (Zingiber officinale var rubra) extract has the highest influence of other activities. It was concluded that giving of garlic and red ginger extract give influence to growth of broiler were infected by Escherichia coli and can be used as alternative antibiotic.


2015 ◽  
Vol 17 (1) ◽  
pp. 49 ◽  
Author(s):  
Ismoyowati Ismoyowati ◽  
Diana Indrasanti ◽  
Mochamad Mufti ◽  
Abdoreza Soleimani Farjam

Abstract. Phytobiotic properties of garlic (Allium sativum), turmeric (Curcuma domestica), red ginger (Zingiber officinale) and kencur (Kaempferia galangal) were studied using standard in vitro antibacterial test and in vivo feeding trial with ducklings. In the in vitro experiment, potency of aqueous extract of these phytobiotic agents were tested against Salmonella pullorum and Escherichia coli. Feeding trial was carried out for 6 week starting at day 28 using ducklings fed diets supplemented with 1% of each of four phytobiotic agents. The highest antibacterial activity against S. pullorum and E. coli was observed with garlic and no additive effect when mixture of phytobiotics was used. Weight gain, fed intake and feed conversion ratio of ducklings were not affected by inclusion of garlic, red ginger and kencur. However, 1% turmeric supplementation significantly reduced growth performance to ducklings. Key words: phytobiotic, antibiotic, duck, medicinal plants Abstrak.  Penelitian karakteristik fitobiotik dari bawang putih (Allium sativum), kunyit (Curcuma domestica), jahe merah (Zingiber officinale) dan kencur (Kaempferia galangal) telah dilakukan secara in vitro melalui uji aktivitas antibakteri dan secara in vivo dengan perlakuan suplemetasi fitobiotik didalam pakan anak itik. Pada percobaan  in vitro, potensi  aktivitas antibakteri dari ektrak  fitobiotik diuji menggunakan Salmonella pullorum and Escherichia coli.  Percobaan suplementasi fitobiotik diberikan masing-masing sebesar 1% didalam pakan anak itik.  Pemberian pakan perlakuan dilakukan selama 6 minggu,  dimulai pada saat anak itik berumur 28 hari.  Hasil penelitian menunjukkan aktivitas antibakteri terhadap S. pullorum dan E. coli paling tinggi adalah ektrak bawang putih dan tidak ada pengaruh yang lebih baik apabila dicampur dengan ektrak fitobiotik lainnya.  Pertambahan bobot badan, konsumsi pakan dan konversi pakan anak itik tidak dipengaruhi oleh penambahan bawang putih, kunyit, jahe merah dan kencur.  Akan tetapi, suplementasi kunyit nyata menurunkan performan pertumbuhan anak itik. Kata kunci: fitobiotik, antibiotik, itik, tanaman obat-obatan.


2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Melisah Yanty Manullang ◽  
Ma`ruf Tafsin ◽  
Iskandar Sembiring ◽  
Tri Hesti Wahyuni ◽  
Hasnudi

This study aims to determine the provision of gambir solution (Uncaria gambir Roxb) to the number of E. coli colonies in broiler chickens. The research method used Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consists of P0A= Control without infection; P0B = (0%), P1=(2%), P2 = (4%), P3 = (6%), P4 = Tetracycline antibiotics (0.05%). The results showed addition of gambir solution (Uncaria gambir Roxb) gave significant effect (P<0,05) to amount of E. coli colony in broiler chicken.    E. coli colonies total in the treatment phase given average for treatment P0A, P0B, P1, P2, P3, P4, sequentially 6.97, 7.80, 6.88, 6.69, 6.34, 6.91 (CFU Log / g). Addition dose with 2-6% of gambir solution effectively reduced the number of    E. coli colonies in broiler chickens. The addition dose of gambir solution 2% was able to compensate tetracycline antibiotics to controlling E. coli in broiler chickens.


2004 ◽  
Vol 67 (9) ◽  
pp. 1829-1833 ◽  
Author(s):  
J. A. CASON ◽  
M. E. BERRANG ◽  
R. J. BUHR ◽  
N. A. COX

Paired carcass halves were used to test whether fecal contamination of skin during processing of broiler chickens can be detected by increased bacterial counts in samples taken before and after immersion chilling. In each of three trials, six freshly defeathered and eviscerated carcasses were cut in half, and a rectangle (3 by 5 cm) was marked with dots of ink on the breast skin of each half. One half of each pair was chosen randomly, and 0.1 g of freshly collected feces was spread over the rectangle with a spatula. After 10 min, both halves were sprayed with tap water for 10 to 15 s until feces could no longer be seen in the marked area. Both halves were sampled with a 1-min carcass rinse and were then put in a paddle chiller with other eviscerated carcasses for 45 min to simulate industrial immersion chilling. Immediately after chilling, each carcass half was subjected to another 1-min rinse, after which the skin within the rectangle was aseptically removed from the carcass halves and stomached. Rinses of fecally contaminated halves had significantly higher Enterobacteriaceae immediately before chilling, but there were no differences in coliform and Escherichia coli counts. After chilling, there were no differences in Enterobacteriaceae, coliform, and E. coli counts in rinse or skin samples from the paired carcass halves. Correlations were generally poor between counts in rinse and skin samples but were significant between prechill and postchill rinses for both control and fecally contaminated halves. Correlations were also significant between counts in rinses of control and contaminated halves of the same carcass after chilling. Bacterial counts in postchill carcass rinses did not indicate that fecal contamination occurred before chilling.


2019 ◽  
Vol 6 (1) ◽  
pp. 8-14
Author(s):  
Nida Nurhanifah ◽  
Sutyarso Sutyarso ◽  
M. Kanedi ◽  
Hendri Busman

The residue of paraquat herbicides may cause human health problems. Paraquat causes the testosterone hormone to decline. Testosterone hormone affects aggressive and sexual behavior. It is caused by free radicals in the body. Free radicals can be non-reactive if in the body there are antioxidants. Red ginger could be used as an antioxidant because it has active phenolic compounds, such as arginine, gingerol, shogaol, zingerone, gingerdiol, and zingiber. Ginger could increase serum testosterone levels. The purpose of this research is to know the effect of red ginger ethanol extract on aggressiveness and libido of male mice induced by paraquat herbicide. This study used a Completely Randomized Design (RAL) consisting of six treatment groups with four replications each. Group P1 as control (aqua dest), P2 (paraquat at dose 20 mg/kg BM), P3, P4, P5 (paraquat with dose 20 mg/kg BM and red ginger extract at 200 mg/kg BM, 400 mg/kg BM, 600 mg/kg BM) and P6 (red ginger extract at 600mg/kg BM). Paraquat is given 2 times a week for 21 days and the red ginger extract is given daily for 35 days. The result of analysis with One-way ANOVA and continued BNT at 5% showed that giving of red ginger ethanol extract can increase aggressive behavior and libido of male mice induced paraquat herbicide.


2021 ◽  
Vol 4 (2) ◽  
pp. 166
Author(s):  
Ndaindila Haindongo ◽  
Amara Anyogu ◽  
Osmond Ekwebelem ◽  
Christian Anumudu ◽  
Helen Onyeaka

Biofilms are a significant concern in the food industry because of their potential to enhance bacterial survival and cause foodborne outbreaks. Escherichia coli (E. coli) is among the leading pathogens responsible for foodborne outbreaks and this can be attributed to its ability to form biofilms in food containers and food preparatory surfaces. The purpose of this study was to investigate the antibacterial and antibiofilm properties of garlic, ginger and mint and their potential to inhibit E.coli and biofilm formation. Disc diffusion assays and 96-well plate crystal violet-based methods were used to achieve these objectives. The plant extracts were diluted from 1 mg/ml to 0.1 mg/ml and incubated 25°C and 37°C to investigate the antimicrobial and antibiofilm effects on E. coli. The findings of this study showed that low temperatures induced the formation of E. coli biofilms and all tested extracts contain a broad spectrum of antibacterial and antibiofilm properties. This study provided new insights on the combined antimicrobial and antibiofilm properties of garlic, ginger and mint against planktonic cells and biofilms of E. coli MG 1655 and highlight the potential use of these extracts in the food industry to prevent biofilm formation by E. coli. 


2021 ◽  
Vol 20 (2) ◽  
pp. 137-142
Author(s):  
Anriani Puspita Karunia Ning Widhi ◽  
Imam Nafi Yana Saputra

Latar belakang: Pemenuhan kebutuhan pangan asal ternak yang berasal dari unggas memiliki angka konsumsi yang cukup tinggi salah satunya yaitu ayam broiler. Untuk memenuhi tingginya permintaan terhadap ayam broiler pada pakandiberifeed additiveserta antibiotic growth promotor (AGP) dalam bentuk antibiotik untuk mempercepat pertumbuhan dan daya tahan tubuh. Pemanfaatan antibiotik yang tidak bijak akan menimbulkan residu antibiotik serta resistensi Escherichia coli penghasil Extended Spectrum β-lactamase (ESBL).Metode: Penelitian ini termasuk penelitian deskriptif. Data primer diperoleh dari hasil screeningtest. Data ditunjukkan dalam tabel dangambar yang dibahas secara deskriptif. Variabel yang diamati residu antibiotik tetrasiklin serta E. coli penghasil ESBL pada daging ayam broiler.Hasil: Temuan terhadap residu antibiotik tetrasiklin pada daging ayam broiler yaitu sebesar 7,14% dengan rerata zona hambat 12,13 mm, sedangkan hasil identifikasi E. coli penghasil ESBL pada daging ayam broiler diperoleh hasil sebesar 71,4%.Simpulan:Residu antibiotik tetrasiklin dalam daging ayam broiler masih tergolong aman dapat dikonsumsi namun, temuanE. coli penghasil ESBL pada daging ayam broiler menimbulkan masalah kesehatan, baik bagi kesehatan hewan maupun manusia, serta dapat menimbulkan resistensi terhadap antibiotik. ABSTRACT Title: Broiler Chicken Meat Sold at Purwokerto's Market Has Antibiotic Residues and Escherichia Coli That Produces EsblBackground: Poultry, of which broiler chickens are one example, is a food source with a relatively high consumption rate.Feed additives and antibiotic growth promoters (AGP) in the form of antibiotics in the ration are given to increase broilers’ growth and endurance to meet the high demand for them. It is important to note that unwise antibiotic use results in the buildup of antibiotic residues and resistance to the Extended Spectrum Beta-lactamase(ESBL)produced by Escherichia coli bacterium.Method: To collect primary data for this descriptive study, the screening test results were employed as a starting point. Next, the information was presented in the form of tables and figures.Result: According to this study, broiler chickens had a tetracycline residue of 7.14 percent, with an inhibitory zone mean of 12.13 mm, and 71.4 percent of ESBL-producing E. coli.Conclusion:According to the study, broiler chicken meat with tetracycline residue is still safe to consume. E. coli, which is known to develop an antibiotic-resistant strain of E. coli ESBL) can cause serious health problems in both humans and animals.


2014 ◽  
Vol 83 (5) ◽  
pp. 225-233 ◽  
Author(s):  
I. Chantziaras ◽  
J. Dewulf ◽  
F. Boyen ◽  
B. Callens ◽  
P. Butaye

In this article, detailed studies on antimicrobial resistance to commensal E. coli (in pigs, meatproducing bovines, broiler chickens and veal calves) and pathogenic E. coli (in pigs and bovines) in Belgium are presented for 2011. Broiler chicken and veal calf isolates of commensal E. coli demonstrated higher antimicrobial resistance prevalence than isolates from pigs and bovines. Fifty percent of E. coli isolates from broiler chickens were resistant to at least five antimicrobials, whereas sixty-one percent of bovine E. coli isolates were susceptible to all antimicrobials tested. On the other hand, bovine pathogenic E. coli isolates showed an extended resistance profile with more than half of the isolates being resistant to ten or more antimicrobials. The results are not significantly different from the results from previously published studies on commensal bacteria in pigs in Belgium, although different methodologies of sampling and susceptibility testing were used.


Author(s):  
Abdul Wahid Jamaluddin ◽  
Muhammad F. Mursalim ◽  
Andi M. S. Apada

Escherichia coli is a gram negative bacterium which is a normal flora in the digestive tract. In birds, this bacterium causes a disease known as colibasillosis. Antibiotics are generally used, but excessive use will cause residues and antibiotic resistance. To avoid resistance or residue, an alternative treatment is needed. The combination of propolis and ginger extract is very promising to develop because both have a synergistic effect as antimicrobials. The research aims to determine the effect of the combination extract on Escherichia coli bacteria in vitro. We used 8 groups which contain combinations of propolis and ginger extract. We used amoxicillin disk as a positive control, 1% Na CMC as a negative control. The results showed a combination of ginger and propolis extract showed good inhibitory activity against Escherichia coli in all groups> 6mm., and the highest inhibitory activity was K3 (5% propolis combination and 15% ginger extract) with 8.7 mm. The combination of propolis and ginger extract has the potential to be used as an alternative antibiotic to prevent antibiotic resistance from synthetic antibiotics.Keywords: alternative antibiotics, combination extracts, Escherichia coli, ginger, in vitro, propolis


2019 ◽  
Vol 9 (1) ◽  
pp. 28-36
Author(s):  
Wuri Marsigit ◽  
Laili Susanti ◽  
Lia Marzalena

ABSTRACTQuality improvement  of sale banana ambon curup was necessary to increase chemical and organoleptics quality and consumer preference. The  purpose  of  this  study  was  to  determine  the  effect  of  variations  in  theconcentration   of   red   ginger   juice   and   the   length   of   immersion   on  chemical   quality, microbiology and organoleptic wet sale of banana ambon curup. By using (RAL) 2 factorial randomized design, the concentration of red ginger juice was 5%, 10% and 15% with a time of immersion for 20 and 10 minutes. The analytical method used was ANOVA. The results of the study found that sucrose levels were 37.56% to 57.54 %, the water content was 36.66% and 38.33%, ash content was 1.47 % to 1.87 %.  The  panelists  preferred  the  average  value  of  the  whole (texture, aroma, and tate)  was  found  in  the  P1T1 sample  with  a  treatment  of  red  ginger  concentration  of  0.05%  and  immersion  time  for  10 minut  


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