scholarly journals BOAR SPERM SURVIVAL ABILITY IN DISTINCTORS OF DIFFERENTLY PRODUCED DISTILLED WATER

2020 ◽  
pp. 115-123
Author(s):  
Irina Martynyuk ◽  
Tetiana Stryzhak ◽  
G. I. Sakhatsky
Keyword(s):  
Artificial Insemination ◽  
Sperm Count ◽  
Survival Rates ◽  
Distilled Water ◽  
Sperm Production ◽  
Boar Semen ◽  
Sperm Survival ◽  
Quantitative Indicators ◽  
Landrace Pig ◽  
Research Show

Testing and comparative evaluation of germ cells in boars of Landrace breed of different genotypes on qualitative and quantitative indicators, for further use in diluents of different distilled water, is carried out. Studies have shown that the quantitative and qualitative indicators of sperm production of French boars had the highest ejaculate volume and sperm count among other boars. Boars of domestic selection had the highest concentration of sperms, and boars of English selection prevailed in terms of motility. The results of research show that among the tested ejaculates the best sperm at a dilution of 1: 1 were found in boars of English Landrace pig breed, the absolute survival rate of which was (Sa) - 732.4 um. from according to the degree of rarefaction 1: 2 - 1: 3, the highest rate had ejaculates of boars of French selection, the studied indicator of which was (Sa) - 720.9 (Sa) - 708.8 um. from in accordance. The use of distilled water of foreign production has improved the survival rates of boar semen compared to distilled water of domestic production, which is produced directly at the artificial insemination point of the farm. Thus, according to this indicator, the semen of domestic boars lived 3.7 hours or 3.4% less than the semen of French boars and 8.8 hours or 5.3 % less than the semen of English boars. The thinning of semen in other degrees did not reveal a probable difference between boars. Analysis of these studies shows that the semen of boars of English selection prevailed on the studied indicator of semen of other boars when used in diluents of water of foreign, domestic and local production (obtained at the point of artificial insemination of the farm).

EFFECTIVENESS OF "SWIM-UP" AND "GRADIENT" METHODS IN SPERM PREPARATION FOR ARTIFICIAL INSEMINATION

2014 ◽  
pp. 25-29
Author(s):  
Minh Tam Le ◽  
Van Trung Nguyen ◽  
Thi Tam An Nguyen ◽  
Quang Trung Phan ◽  
Ngoc Thanh Cao
Keyword(s):  
Gradient Method ◽  
Artificial Insemination ◽  
Sperm Count ◽  
Gradient Methods ◽  
Infertility Treatment ◽  
University Hospital ◽  
Preparation Methods ◽  
Sperm Preparation ◽  
Abnormal Morphology ◽  
Sperm Washing

Objectives: Artificial insemination with sperm preparation and inseminate to the uterus is a common method of infertility treatment. Currently two methods used for sperm washing is “swim-up” and “gradient”. Materials and methods:cross-sectional description in 166 cycles of artificial insemination in Hue University Hospital from April, 2012 to March, 2013 in order to compare effectiveness between “swim-up” and “gradient” methods. Samples were collected randomly into two methods. Results:Results of sperm preparation in both methods are equivalent in terms of the following parameters total sperm count, total number of progressive sperm, total good morphology sperm. However, “gradient” method results in higher number of progressive sperm in case with slow motility (38.3% vs. 26.1%) or abnormal morphology (34.9% vs. 19.7 %) compared with “swim-up” method. Pregnancy rates after artificial insemination were similar between two preparation methods. Conclusion:In case of slow motility sperm and abnormal morphology, “gradient” method should be used to increase the number of progressive sperms. Pregnancy outcome depends on many factors other than preparation methods. Key words: Artificial insemination, sperm preparation, infertility treatment

SERUM GONADOTROPHINS, TESTOSTERONE AND SPERMATOGENESIS IN SUBFERTILE MEN

1977 ◽  
Vol 86 (3) ◽  
pp. 651-658 ◽  
Author(s):  
J. H. Aafjes ◽  
J. C. M. van der Vijver ◽  
R. Docter ◽  
P. E. Schenck
Keyword(s):  
Partial Correlation ◽  
Sperm Count ◽  
Correlation Coefficients ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
The Other ◽  
Sperm Production ◽  
Positive Correlation ◽  
Natural Logarithm ◽  
Biopsy Score

ABSTRACT In 210 subfertile men there existed a significant positive correlation between serum FSH and LH (0.41). No correlation was observed between the gonadotrophin levels and testosterone. In contrast to this FSH as well as LH were negatively correlated with the natural logarithm (In) of the sperm count/ml ejaculate (−0.44 and −0.18, respectively). When the positive correlation which existed between FSH and LH was used to calculate partial correlation coefficients, the coefficient between FSH and ln sperm count did hardly change (−0.41) the coefficient between LH and ln sperm count on the other hand became insignificant (−0.05). This suggests that spermatogenesis influences FSH serum levels in subfertile men by a decreased suppression when sperm production is diminished. Testicular biopsies taken from 97 of these patients were used to determine biopsy scores. These scores showed a significant negative correlation with FSH (−0.34) and a positive one with ln sperm count/ml ejaculate (0.45). Interestingly the biopsy score of 16 patients who fertilized their wives, was found to be higher compared with the score of the other patients who did not fertilize. The number of sperm/ml ejaculate and the FSH values of these 2 groups of biopsied patients were, however, not significantly different. This leads to the conclusion that the biopsy score is a better parameter for the evaluation of oligospermic men than either sperm count or FSH serum values.

scholarly journals Fertilisation rate obtained with frozen-thawed boar semen supplemented with rosmarinic acid using a single insemination timed according to vulvar skin temperature changes

2015 ◽  
Vol 63 (1) ◽  
pp. 100-109 ◽  
Author(s):  
Victoria Luño ◽  
Lydia Gil ◽  
Maite Olaciregui ◽  
Juan Grandía ◽  
Trinidad Ansó ◽  
...  
Keyword(s):  
Skin Temperature ◽  
Artificial Insemination ◽  
Rosmarinic Acid ◽  
Intrauterine Insemination ◽  
Fertility Rates ◽  
Temperature Changes ◽  
Progressive Motility ◽  
A Value ◽  
Fertilisation Rate ◽  
Boar Semen

Artificial insemination (AI) of sows with frozen-thawed semen usually results in lower pregnancy rates and litter sizes than the use of liquid preserved semen. The present study evaluated the effectiveness of vulvar skin temperature changes as a predictor of ovulation in sows and determined the fertility rates obtained after AI with frozen-thawed semen supplemented with rosmarinic acid (RA). Semen was collected from mature boars and cryopreserved in experimental extenders supplemented with or without 105 μM of RA. Multiparous sows were inseminated with a single dose of semen when vulvar skin temperature decreased to a value below 35 °C. Intrauterine insemination was performed using 1.5 × 109 spermatozoa. The sows were slaughtered 48 h after AI and the embryos and oocytes were recovered from the oviducts. Total and progressive motility, viability and acrosome integrity were significantly (P < 0.05) higher in RA-supplemented semen samples compared with the control. Fertilisation occurred in all sows inseminated in the study, although there were no significant differences between the experimental groups. Sows inseminated with RA-supplemented semen showed a slight increase in the number of embryos recovered as compared to sows inseminated with control semen. In conclusion, insemination according to vulvar skin temperature changes resulted in successful fertilisation in all sows, although supplementation of the freezing media with RA did not improve the fertilising ability of frozen-thawed boar sperm.

scholarly journals Environmental and Nutritional Aspect in Male Infertility

1970 ◽  
Vol 10 (1) ◽  
pp. 16-19 ◽  
Author(s):  
Hamida Begum ◽  
ABM Moniruddin ◽  
Khairun Nahar
Keyword(s):  
Heavy Metals ◽  
Male Infertility ◽  
Sperm Count ◽  
Sperm Production ◽  
Male And Female ◽  
Exogenous Estrogen ◽  
Sperm Counts ◽  
And Function ◽  
Nutritional Aspect ◽  
Anatomical Defects

Male and female partner of a couple must be standard and fit to have the capacity to procreate. Studies confirm that male sperm counts are declining and environmental factors as pesticides, exogenous estrogen, heavy metals negatively impact spermatogenesis without any obvious anatomical defects. So, a number of nutritional therapies have been shown to improve sperm count and motility as carnitine, arginine, zinc, selenium and vitamin B12. Numerous anitioxidants have prove beneficial in treating male infertility as Vitamin C, Vitamin E, Glutathione and Coenzyme Q10. This article aims to highlight the correction of nutritional imbalances to encourage optimum sperm production and function, when there is idiopathic impaired spermatogenesis.   doi:10.3329/jom.v10i1.1997   J Medicine 2009; 10: 16-19   

Modification of spermatozoa quality in mature small ruminants

2012 ◽  
Vol 24 (1) ◽  
pp. 13 ◽  
Author(s):  
G. B. Martin ◽  
T. Jorre de St Jorre ◽  
F. A. Al Mohsen ◽  
I. A. Malecki
Keyword(s):  
Large Body ◽  
Small Ruminants ◽  
Cell Loss ◽  
Testicular Tissue ◽  
The Other ◽  
Sperm Production ◽  
Testicular Mass ◽  
Other Hand ◽  
Sperm Survival ◽  
Sperm Output

This review is based largely, but not entirely, on the assumption that gamete quality is directly linked to sperm output and thus testicular mass, an approach made necessary by the absence of a large body of data on factors that affect gamete quality in ruminants. On the other hand, there is a change in the efficiency of sperm production per gram of testicular tissue when the testis is growing or shrinking, a clear indicator of changes in the rates of cell loss during the process of spermatogenesis, probably through apoptosis. We therefore postulate that the spermatozoa that do survive when the testis is shrinking are of a lower quality than those that are produced when the testis is growing and the rate of sperm survival is increasing. In adult small ruminants in particular, testicular mass and sperm production are highly labile and can be manipulated by management of photoperiod (melatonin), nutrition, genetics and behaviour (‘mating pressure’). Importantly, these factors do not act independently of each other – rather, the outcomes in terms of sperm production are dictated by interactions. It therefore seems likely that spermatozoa quality will be affected by these same factors, but definitive answers await detailed studies.

scholarly journals REPRODUCING QUALITIES OF RAMS OF THE MOLDOVAN TYPE KARAKUL BREED

2020 ◽  
Vol 59 ◽  
pp. 92-96
Author(s):  
D. Rotari
Keyword(s):  
Sperm Count ◽  
Sperm Concentration ◽  
Animal Husbandry ◽  
Sperm Production ◽  
Livestock Farming ◽  
High Quality ◽  
Average Percentage ◽  
Reproductive Ability ◽  
First Time

Issues related to the reproduction of animals have been and remain one of the most complex and relevant problems of biology and are constantly finding a direct and effective way out into livestock farming practice. The rational use of breeding sheep as producers is limited by the lack of standard, objective methods and methods for the timely assessment of their reproductive ability. The article presents the results of evaluating the sperm production of rams-producers. For the first time, the freshly obtained sperm of rams of Moldavian type producers of the Karakul breed was evaluated using the macroscopic method - ejaculate volume, color and smell, as well as the microscopic method - motility, sperm concentration in the ejaculate, total sperm count in the ejaculate sperm movements (VAP; VSL and VCL) as well as the percentage of abnormal forms of sperm. The experiments were carried out on sheep producers grown on a pedigree farm of the experimental farm of the Moldavian Scientific and Practical Institute of Biotechnology in Animal Husbandry and Veterinary Medicine. As a result of the studies, it was found that the ejaculate volume on average was 0.99 ± 0.04 ml, motility was 0.95 ± 0.02 and sperm concentration 1.51 ± 0.14 billion/ml. The percentage of pathological forms in sperm averaged 13.72 ± 0.61, an indicator that characterizes the high quality of sperm. The average quality indicator of ejaculates obtained from ram-producers of the Moldavian type of the Karakul breed corresponds to physiological standards for the Karakul breed. The average percentage of pathological forms of sperm found in ejaculates indicates that the rams were in good conditions of feeding and keeping. According to research, we can say that the Moldovan type of Karakul rams can be successfully used to obtain high quality ejaculates suitable for freezing sperm at 196°C.

scholarly journals Semen quality and diversity of morphological sperm abnormalities in bulls: breed and strain effects

2019 ◽  
Vol 22 (8) ◽  
pp. 931-938
Author(s):  
M. A. Kleshchev ◽  
V. L. Petukhov ◽  
L. V. Osadchuk
Keyword(s):  
Sperm Motility ◽  
Environmental Conditions ◽  
Western Siberia ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Sperm Head ◽  
Sperm Dna Fragmentation ◽  
Sperm Production ◽  
Sperm Abnormalities

At present great attention is paid to studying genetic regulation of farm animal adaptations to environmental conditions. This problem is very important due to a wide expansion of highly productive cattle breeds created in Europe and North America. However, until the present no investigation of changing semen quality in bulls of imported breeds during their adaptations to environmental conditions of Western Siberia has been conducted. The aim of this study was to investigate semen quality peculiarities and the diversity of morphological sperm abnormalities in bulls of imported and local breeds kept in the environmental conditions of the southern part of Western Siberia. We determined sperm concentration, sperm count, and rate of sperm with progressive motility and percentage of morphologically normal spermatozoa. The rate of sperm abnormalities according to Blome’s classifcation was determined too. It was found that the mean values of sperm concentration, sperm motility and percentage of morphologically normal spermatozoa in the bulls investigated were similar to those in bulls kept in European countries. Inter­breed differences in these parameters were not found. However, bulls of the Red Danish, Angler, and Simmental breeds had a higher percentage of misshapen sperm head and pyriform sperm head than bulls of the Black­White breed. An inter­strain difference in sperm motility in bulls of the Black­White breed was observed. It was found that bulls of Reflection Sovereign 198998 strain had lover sperm motility than bulls of Wis Burke Ideal 1013415 strain. No inter­strain differences in sperm production, percentage of morphologically normal spermatozoa and rate of main sperm abnormalities were found. Thus, it has been found that the environmental conditions of the southern part of Western Siberia do not seriously affect the sperm production, sperm motility or percentage of morphologically normal spermatozoa in bulls. However, the increased rate of misshapen and pyriform sperm heads in the bulls of the foreign breeds points to a need to study sperm DNA fragmentation.

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Zygote ◽  
2015 ◽  
Vol 24 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Tao Shen ◽  
Zhong-Liang Jiang ◽  
Cong-Jun Li ◽  
Xiao-Chen Hu ◽  
Qing-Wang Li
Keyword(s):  
Lipoic Acid ◽  
Artificial Insemination ◽  
Antioxidant Effect ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Alpha Lipoic Acid ◽  
Glutamic Oxaloacetic Transaminase ◽  
Boar Semen ◽  
Endogenous Antioxidant ◽  
Boar Spermatozoa

SummaryAlpha-lipoic acid (ALA) is known to be a natural antioxidant. The aim of the present study was to evaluate the cryoprotective effect of ALA on the motility of boar spermatozoa and its antioxidant effect on boar spermatozoa during freezing–thawing. Different concentrations (2.0, 4.0, 6.0, 8.0 or 10.0 mg/ml) of ALA were added to the extender used to freeze boar semen, and the effects on the quality and endogenous antioxidant enzyme activities of frozen–thawed spermatozoa were assessed. The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post-thaw (P < 0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxaloacetic transaminase and catalase improved after adding ALA to the extender (P < 0.05). Artificial insemination results showed that pregnancy rate and litter size were significantly higher at 6.0 mg/ml in the ALA group than in the control group (P < 0.05). In conclusion, ALA conferred a cryoprotective capacity to the extender used for boar semen during the process of freezing–thawing, and the optimal concentration of ALA for the frozen extender was 6.0 mg/ml.

THE EFFECT OF NORADRENALINE ON THE SURVIVAL OF RATS SUBJECTED TO HEMORRHAGIC SHOCK

1957 ◽  
Vol 35 (1) ◽  
pp. 93-101 ◽  
Author(s):  
A. M. Lansing ◽  
J. A. F. Stevenson ◽  
C. W. Gowdey
Keyword(s):  
Survival Rate ◽  
Hemorrhagic Shock ◽  
Survival Time ◽  
Intravenous Infusion ◽  
Survival Rates ◽  
Distilled Water ◽  
Sprague Dawley ◽  
Chi Square ◽  
Sprague Dawley Rats ◽  
Chi Square Test

Reports of the efficacy of l-noradrenaline in the treatment of clinical shock stimulated an investigation of its effect in controlled hemorrhagic hypotension. Seventy-three 350-g. male Sprague–Dawley rats were subjected to a standardized hemorrhagic shock procedure. Of 15 control animals that received no treatment, only one survived for 48 hours; none survived of the five controls that received a constant intravenous infusion, after the shock procedure, of 5% glucose in distilled water until death or for 36 hours. The treated animals received, after the shock procedure, an infusion of l-noradrenaline (0.5–2.0 μg./min.) in 5% glucose in distilled water. The survival rates for the treated animals were: treatment for 1 hour, 1/8; treatment for 4 hours, 4/15; treatment until death or for 36 hours, 8/15. Fifteen animals received, in addition to noradrenaline for 36 hours, hydrocortisone administered intravenously (0.7 μg./min.) or intramuscularly (2.5 mg. every 6 hours); seven of these animals survived.Analysis of variance showed that there was no difference in the shock procedure undergone by the controls and by the treated survivors. The Chi square test on the survival rates revealed that the infusion of noradrenaline for 1 hour or 4 hours did not improve survival, but infusion for 36 hours produced a very significant increase in survival time and in total survival rate. The addition of hydrocortisone neither enhanced nor impaired this improvement.

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