FEATURES OF CHANGE OF QUALITATIVE AND QUANTITATIVE COMPOSITION OF MICROBIOCENOSIS OF ORAL CAVITY DEPENDING ON THE EFFECT OF DENTAL HYGIENE

2019 ◽  
pp. 85-94
Author(s):  
Kaznachieieva М.S. ◽  
Bohdan A.M.
Keyword(s):  
Oral Cavity ◽  
Dental Hygiene ◽  
Quantitative Composition ◽  
Qualitative And Quantitative

scholarly journals MICROFLORA OF THE ORAL CAVITY AND CONDITION OF ANTI-INFECTION PROTECTION FACTORS IN PERSONS USING DENTAL ORTHOPEDIC CONSTRUCTIONS (DOC)

2018 ◽  
pp. 92-98
Author(s):  
Yu. S. Shishkova ◽  
M. S. Babikova ◽  
A. S. Emelina ◽  
O. I. Filimonova ◽  
D. A. Tezikov ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Oral Fluid ◽  
Infectious Complications ◽  
Blue Staining ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Dependent Potential ◽  
Pcr Method ◽  
The Stability

Aim. To study the qualitative composition, the quantitative content of microorganisms, to assess the viability and functional status of neutrophils in the oral fluid of patients using removable and non-removable DOC. Materials and methods. The present study used a free oral fluid of 100 patients aged 21 to 79 years. The qualitative and quantitative composition of the microflora was determined using the PCR method by means of the Femoflor-16 system (NPO DNA-technology, Russia). Determination of the total number of leukocytes was carried out in Goryaev's chamber, analysis of their viability was carried out with Trypan blue staining. Acridine orange was used to study the indices of lysosomal activity of neutrophils. Oxygendependent metabolism was studied using the Nitro Blue-Tetrazolium (NBT) test, and the phagocytic function was determined by the absorption of latex particles. Results. In persons using dentures, the qualitative and quantitative composition of the microflora of the oral cavity changes. The method of hygienic care of the DOC significantly does not affect the composition of the microflora of the oral fluid of patients. In persons using DOC, a decrease in the total number of leukocytes and their viable forms in the free oral fluid was established. However we registered the stability of functioning of viable neutrophils in patients with removable DOC and a significant decrease in the phagocytic ability and microbicidal oxygen-dependent potential of neutrophils when using non-removable DOC. Conclusion. Foreign body in the form of a removable or non-removable DOC changes the functioning of the factors of congenital anti-infective protection of the oral mucosa, which can lead to the development of infectious complications during dental prosthetics.

scholarly journals CHARACTERISTICS OF THE MICROBIOM OF THE NECK REGION OF THE TEETH IN THE EXPERIMENTAL ACTION OF OPIOID, ITS CANCELLATION AND APPLICATION OF CEFTRIAXONE AS A COMPOSITION OF COMPLEX MEDICAL CORRECTION

2020 ◽  
Author(s):  
Fik V. B. ◽  
Fedechko Y.M. ◽  
Palʹtov Ye. V.
Keyword(s):  
Oral Cavity ◽  
Antibiotic Therapy ◽  
Opioid Analgesic ◽  
Bacterial Species ◽  
Neck Region ◽  
Quantitative Composition ◽  
Antibacterial Drug ◽  
Qualitative And Quantitative ◽  
Gingival Margin ◽  
Neck Part

The aim of the study was to determine the changes in the qualitative and quantitative composition of the microbiota in the neck part of the teeth in the gingival margin of the oral cavity of rats under experimental opioid exposure, its cancellation and comprehensive drug correction using an antibacterial drug. The use of the antibiotic ceftriaxone under conditions of two- and six-week administration of the opioid analgesic nalbuphine, caused the elimination or reduction of the quantitative composition of opportunistic and pathogenic bacterial species, in contrast to the identified changes in the microbiocenosis in the studied microbiotope. antibiotic therapy in the experiment.

A new approach to treatment of stomatitis in experiments on white rats (experimental research)

2018 ◽  
pp. 168-173
Author(s):  
Е.Б. Ганина ◽  
Ю.В. Червинец ◽  
Н.В. Грудинин ◽  
В.Г. Шестакова ◽  
В.М. Червинец ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Oral Cavity ◽  
Experimental Research ◽  
Quantitative Composition ◽  
Oral Diseases ◽  
New Approach ◽  
Highly Active ◽  
White Rats ◽  
Before And After ◽  
Intact Rats

Цель исследования - охарактеризовать микробиологическую картину стоматита до и после его лечения высокоактивными культурами лактобацилл, дать оценку эффективности их применения при экспериментальном стоматите. Методика. Опыты проводились на 32 самках беспородных белых крыс массой 230 г. Моделирование стоматита включало 2 этапа: моделирование травматического стоматита и воспроизведение бактериального стоматита на базе травматического. У крыс контрольной и опытных серий на всех этапах эксперимента брали мазки с поверхности десен для характеристики микробиоценоза ротовой полости. Результаты. После обработки слизистой оболочки полости рта (СОПР) культурой патогенного штамма Staphylococcus aureus существенно снизились частота встречаемости и количество условно-патогенных микроорганизмов. Лечение стоматита у крыс культурами лактобацилл ( Lactobacillus 11 зв., Lactobacillus 2 п.рта, Lactobacillus 24 д.ст.) и их комбинацией приводило к снижению распространенности S. aureus вплоть до его исчезновения, а также к нормализации количества условно-патогенной микробиоты. Заключение. Исследования показали эффективность использования отдельных высокоактивных штаммов лактобацилл или их комбинации при лечении заболеваний СОПР, в частности бактериального стоматита. Aim. To characterize the microbiological picture of stomatitis in intact rats before and after the treatment with highly active cultured lactobacilli and to evaluate the effectiveness of this treatment in experiments on white rats. Methods. Experiments were carried out on 32 mongrel female white rats weighing 230 g. Smears were taken from the gum surface of control and experimental rats at all stages of the experiment to characterize the oral cavity microbiocenosis. Results. After treatment of the oral cavity with cultured Staphylococcus aureus , the occurrence and quantity of opportunistic microorganisms significantly decreased. The treatment of stomatitis in rats with cultured lactobacillus ( Lactobacillus 11 zv., Lactobacillus 2 p.r., Lactobacillus 24 d.st. and their combination) led to a decrease in S. aureus prevalence up to its extinction and to normalization of the quantitative composition of opportunistic microbiota. Conclusions. The study showed the effieacy of highly active lactobacillus strains individually or their combinations in the treatment of oral diseases, in particular, bacterial stomatitis.

scholarly journals Anti-oxidative and antimicrobial activities of Hieracium pilosella L. extracts

2008 ◽  
Vol 73 (5) ◽  
pp. 531-540 ◽  
Author(s):  
Ljiljana Stanojevic ◽  
Mihajlo Stankovic ◽  
Vesna Nikolic ◽  
Ljubisa Nikolic
Keyword(s):  
Salmonella Enteritidis ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Quantitative Composition ◽  
Minimal Inhibitory Concentrations ◽  
Qualitative And Quantitative ◽  
Scavenging Effect ◽  
Hieracium Pilosella ◽  
Radical Scavenging Effect

The anti-oxidative and antimicrobial activities of different extracts from Hieracium pilosella L. (Asteraceae) whole plant were investigated. The total dry extracts were determined for all the investigated solvents: methanol, dichloromethane, ethyl acetate and dichloromethane: methanol (9:1). It was found that the highest yield was obtained by extraction with methanol (12.9 g/100 g of dry plant material). Qualitative and quantitative analysis were performed by the HPLC method, using external standards. Chlorogenic acid, apigenin-7-O-glucoside and umbelliferone were detected in the highest quantity in the extracts. The qualitative and quantitative composition of the extracts depends on the solvent used. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in the methanolic extract, both with and without incubation, EC50 = 0.012 and EC50 = 0.015 mg ml-1, respectively. The antimicrobial activities of the extracts towards the bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Klebsiella pneumoniae) and the fungi (Aspergillus niger and Candida albicans) were determined by the disc diffusion method. The minimal inhibitory concentrations were determined for all the investigated extracts against all the mentioned microorganisms.

scholarly journals Chemistry and Biology of Essential Oils of GenusBoswellia

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Hidayat Hussain ◽  
Ahmed Al-Harrasi ◽  
Ahmed Al-Rawahi ◽  
Javid Hussain
Keyword(s):  
Essential Oils ◽  
Current Knowledge ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Quantitative Composition ◽  
Volatile Constituents ◽  
Traditional Medicines ◽  
Qualitative And Quantitative ◽  
Synthetic Drugs ◽  
Explicit Consideration

The properties ofBoswelliaplants have been exploited for millennia in the traditional medicines of Africa, China, and especially in the Indian Ayurveda. In Western countries, the advent of synthetic drugs has obscured the pharmaceutical use ofBoswellia, until it was reported that an ethanolic extract exerts anti-inflammatory and antiarthritic effects. Frankincense was commonly used for medicinal purposes. This paper aims to provide an overview of current knowledge of the volatile constituents of frankincense, with explicit consideration concerning the diverseBoswelliaspecies. Altogether, more than 340 volatiles inBoswelliahave been reported in the literature. In particular, a broad diversity has been found in the qualitative and quantitative composition of the volatiles with respect to different varieties ofBoswellia. A detailed discussion of the various biological activities ofBoswelliafrankincense is also presented.

The control of the qualitative and quantitative composi-tion of vitamins and tocopherols of various types of vegetable oils

2021 ◽  
pp. 92-95
Author(s):  
Александр Николаевич Остриков ◽  
Наталья Леонидовна Клейменова ◽  
Инэсса Николаевна Болгова ◽  
Максим Васильевич Копылов ◽  
Екатерина Юрьевна Желтоухова
Keyword(s):  
Vegetable Oils ◽  
The Body ◽  
Quantitative Composition ◽  
Vitamin B ◽  
Silybum Marianum ◽  
Qualitative And Quantitative ◽  
Energy Needs ◽  
Wide Range ◽  
Cold Pressed ◽  
Vitamin Activity

Использование растительных масел в рационе человека необходимо для удовлетворения энергетической потребности организма и регулирования биологических процессов. В настоящей работе представлен качественный и количественный состав витаминов и токоферолов различных видов растительных масел, полученных холодным отжимом из выращенных в нашей стране расторопши, горчицы, подсолнечника, рыжика, рапса. Проведен сравнительный анализ литературных источников о наличии витаминов и токоферолов в различных растительных маслах. Количественный и качественный витаминный состав для одного и того же вида масла, по данным разных авторов, варьируется в достаточно широком диапазоне. В ходе исследования изучен витаминный состав пяти растительных масел. Результаты свидетельствуют, что наибольшей витаминной активностью обладают масла рыжиковое, расторопши и горчичное. Установлено наличие витамина А в маслах: рыжиковом (27,15±0,002 мкг%), расторопши (19,07±0,02 мкг%), горчичном (24,77±0,02 мкг%). Витамины В и В присутствуют в маслах горчичном, рыжиковом и расторопши; витамин В определен в горчичном и рыжиковом, В - в рыжиковом и расторопши. В маслах горчичном и расторопши присутствует витамин В. Наиболее богаты витамином Е масла рыжиковое (52,8±0,02 мг%), подсолнечное (48,3±0,02 мг%), расторопши (47,12±0,02 мг%). Витамин К и токоферолы определены во всех растительных маслах. Присутствие b-каротина обнаружено в рыжиковом (1,237±0,004 мг%), расторопши (0,812±0,002 мг%), подсолнечном (0,22±0,02 мг%) и горчичном (0,148±0,002 мг%) маслах. Анализируемые масла холодного отжима можно рассматривать как ценный источник при проектировании новых многоцелевых продуктов или побочных продуктов для промышленного, косметического и фармацевтического использования. The use of vegetable oils in the human diet is necessary to meet the energy needs of the body and regulate biological processes. This work presents the qualitative and quantitative composition of vitamins and tocopherols of various types of vegetable oils obtained by cold pressing from silybum marianum, mustard, sunflower, camelina, rapeseed grown in our country. A comparative analysis of the literature on the presence of vitamins and tocopherols in various vegetable oils has been carried out. The quantitative and qualitative vitamin composition for the same type of oil, according to different authors, varies in a wide range. During the study, the vitamin composition of five vegetable oils was studied. The results indicate that the following oils have the highest vitamin activity: camelina, silybum marianum and mustard. The presence of vitamin A was found in oils: camelina (27.15±0.002 μg%), silybum marianum (19.07±0.02 μg%), mustard (24.77±0.02 μg%). Vitamins B and B are present in mustard, camelina and silybum marianum oils; vitamin B is found in mustard and camelina; B in camelina and silybum marianum. Mustard and silybum marianum oils contain vitamin B. The richest in vitamin E are camelina (52.8±0.02 mg%), sunflower (48.3±0.02 mg%), silybum marianum (47.12±0.02 mg%). Vitamin K and tocopherols are found in all vegetable oils. The presence of b-carotene was found in camelina (1.237±0.004 mg%), silybum marianum (0.812±0.002 mg%), sunflower (0.22±0.02 mg%) and mustard (0.148±0.002 mg%) oils. The analyzed cold-pressed oils can be considered as a valuable resource when designing new multipurpose products or by-products for industrial, cosmetic and pharmaceutical applications.

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Macrozoobenthos of the Moravice river and Bělokamenný potok brook

2006 ◽  
Vol 54 (4) ◽  
pp. 75-80 ◽  
Author(s):  
Ivo Sukop
Keyword(s):  
Small Town ◽  
Quantitative Composition ◽  
Saprobic Index ◽  
Qualitative And Quantitative ◽  
Density And Biomass

Qualitative and quantitative composition of macrozoobenthos was studied on the Moravice river (six sites) and Bělokamenný potok brook (one site). Altogether, 64 and 29 taxa of water macroinvertebrates were determined in the Moravice river and Bělokamenný potok brook respectively. Saprobic index on the majority of monitored localities corresponded to oligosaprobity. On the sites 2 and 3 (downstream of the small town of Břidličná), the saprobic index showed β-mesosaprobity. The values of macrozoobenthos density and biomass ranged between 317–605 ind.m–2 and 4.1–6.5 g.m–2 respectively.

scholarly journals EDUKASI ONLINE TERHADAP PERUBAHAN PERILAKU MENJAGA KEBERSIHAN GIGI PADA MULUT SELAMA MASA PANDEMI

2021 ◽  
Vol 16 (1) ◽  
pp. 224-228
Author(s):  
Ajeng yumna Azizah
Keyword(s):  
High School ◽  
Online Education ◽  
Oral Cavity ◽  
Behavior Change ◽  
Dental Hygiene ◽  
Systemic Disease ◽  
Senior High School ◽  
T Test ◽  
Cross Sectional ◽  
Hygiene Behavior

ABSTRACT Oral cavity is one of the crucial parts of our body. Oral cavity represents our oral health because there are a lot of disease symptoms from our oral cavity. Systemic disease could cause poor oral and dental health. Online education is one way to improve someone’s oral and dental hygiene level. Online education is one of learning process with the internet as a media, so the users can connect with everybody, whenever and wherever they want. Purpose of this research is to determine the effect of online education towards students’ oral and dental hygiene behavior change in YPSA Senior High School Medan during COVID-19 pandemic. This research was conducted with an observational analytic method with cross sectional design with a total sample of 55 students. Sample was determined by accidental sampling technique. Data was analyzed using a statistics dependent t test. Results showed that there were any behavior changes before and after online education. Conclusion of this research showed there were any significant effect of online education towards students’ oral and dental hygiene behavior change in YPSA Senior High School Medan during COVID-19 pandemic.     ABSTRAK Mulut merupakan bagian krusial bagi tubuh, selain ini dikatakan juga bahwa mulut merupakan cermin dari Kesehatan tubuh, karena dari mulut dapat dilihat gejala beberapa penyakit umum yang lainnya. Gangguan sistemik dapat disebabkan oleh kondisi gigi serta mulut yang kebersihannya buruk. Edukasi online dapat menjadi salah satu cara yang dapat dilakukan untuk meningkatkan tingkat kebersihan gigi serta mulut pada seseorang. Edukasi online adalah suatu proses belajar mengajar yang memakai jaringan internet yang dapat memudahkan penggunanya terhubung tanpa keterbatasan waktu dan tempat dengan siapa saja. Tujuan penelitian ini adalah untuk mengetahui pengaruh edukasi online terhadap perubahan perilaku menjaga kebersihan gigi pada mulut selama masa pandemi di SMA YPSA Medan. Jenis penelitian ini merupakan penelitian obeservasional analitikmenggunakan desain cross sectional. Jumlah sampel 55 orang. Penentuan sampel memakai Teknik Accidental sampling. Uji analisis yang dipakai yaitu dependent t test. Hasil penelitian menunjukkan terdapat perubahan perilaku menjaga kebersihan gigi pada mulut sebelum dan sesudah edukasi online. Kesimpulan penelitian ini yaitu ada pengaruh edukasi online terhadap perubahan perilaku menjaga kebersihan gigi pada selama masa pandemi pada di SMA YPSA Medan.  

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