scholarly journals MICROFLORA OF THE ORAL CAVITY AND CONDITION OF ANTI-INFECTION PROTECTION FACTORS IN PERSONS USING DENTAL ORTHOPEDIC CONSTRUCTIONS (DOC)

2018 ◽  
pp. 92-98
Author(s):  
Yu. S. Shishkova ◽  
M. S. Babikova ◽  
A. S. Emelina ◽  
O. I. Filimonova ◽  
D. A. Tezikov ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Oral Fluid ◽  
Infectious Complications ◽  
Blue Staining ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Dependent Potential ◽  
Pcr Method ◽  
The Stability

Aim. To study the qualitative composition, the quantitative content of microorganisms, to assess the viability and functional status of neutrophils in the oral fluid of patients using removable and non-removable DOC. Materials and methods. The present study used a free oral fluid of 100 patients aged 21 to 79 years. The qualitative and quantitative composition of the microflora was determined using the PCR method by means of the Femoflor-16 system (NPO DNA-technology, Russia). Determination of the total number of leukocytes was carried out in Goryaev's chamber, analysis of their viability was carried out with Trypan blue staining. Acridine orange was used to study the indices of lysosomal activity of neutrophils. Oxygendependent metabolism was studied using the Nitro Blue-Tetrazolium (NBT) test, and the phagocytic function was determined by the absorption of latex particles. Results. In persons using dentures, the qualitative and quantitative composition of the microflora of the oral cavity changes. The method of hygienic care of the DOC significantly does not affect the composition of the microflora of the oral fluid of patients. In persons using DOC, a decrease in the total number of leukocytes and their viable forms in the free oral fluid was established. However we registered the stability of functioning of viable neutrophils in patients with removable DOC and a significant decrease in the phagocytic ability and microbicidal oxygen-dependent potential of neutrophils when using non-removable DOC. Conclusion. Foreign body in the form of a removable or non-removable DOC changes the functioning of the factors of congenital anti-infective protection of the oral mucosa, which can lead to the development of infectious complications during dental prosthetics.

scholarly journals Influence of pressure and time on extraction process using supercritical CO2

2008 ◽  
Vol 44 (1) ◽  
pp. 125-131 ◽  
Author(s):  
V. Micic ◽  
Z. Lepojevic ◽  
B. Mandic ◽  
M. Jotanovic ◽  
G. Tadic ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Co2 ◽  
Particle Diameter ◽  
Extraction Process ◽  
Salvia Officinalis ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Salvia Officinalis L ◽  
Carbon Dioxide Co2

The supercritical fluid extraction (SFE) by carbon dioxide (CO2) of Salvia officinalis L. was investigated. SFE by CO2 was performed at different pressure (80, 100, 150, 200 and 300 bar) and constant temperature of 40?C (all other extraction conditions, such are flow rate, particle diameter of Salvia officinalis, extraction time were kept constant. The GC-MS method was used for determination of qualitative and quantitative composition of obtained extracts and essential oils.

scholarly journals THE COMPLEX METHOD FOR DETERMINATION OF INTESTINAL MICROFLORA COMPOSITION OF LABORATORY ANIMALS

2015 ◽  
Vol 22 ◽  
pp. 60-65
Author(s):  
M. V. Kaminska
Keyword(s):  
Intestinal Microflora ◽  
Laboratory Animals ◽  
Albino Rats ◽  
Quantitative Composition ◽  
Complex Method ◽  
Qualitative And Quantitative ◽  
Method Of Determination ◽  
Full Picture

The method of determination of qualitative and quantitative composition of intestinal microflora of laboratory animals, namely albino rats, was described. The method allows to simultaneously identify different groups of microorganisms in one sample of intestinal composition and to get a full picture of microbocoenosis within 4 days that allows to consider animal’s health, dysbiotic infringement in intestinal microflora composition and its correction with probiotic preparations.

scholarly journals Essential oil and extract of coriander (Coriandrum sativum L.)

2011 ◽  
pp. 281-288 ◽  
Author(s):  
Zoran Zekovic ◽  
Dusan Adamovic ◽  
Gordana Cetkovic ◽  
Marija Radojkovic ◽  
Senka Vidovic
Keyword(s):  
Essential Oil ◽  
Steam Distillation ◽  
Methylene Chloride ◽  
Soxhlet Extraction ◽  
Coriandrum Sativum ◽  
Geranyl Acetate ◽  
Quantitative Composition ◽  
Dry Extract ◽  
Qualitative And Quantitative

Two different methods of coriander (Coriandrum sativum L.) essential oil isolation, steam distillation and extraction by methylene chloride (Soxhlet extraction) were investigated. After the determination of essential oil content in the investigated drug and in dry extract (using steam distillation), qualitative and quantitative composition of obtained essential oils, determined by TLC and GC-MS methods, were compared. The content of linalool was higher (52.4%) in essential oil obtained by coriander steam distillation than that in essential oil separeted from dry extract (42.8%), and, on the other hand, content of geranyl-acetate was lower (4.6% and 11.7%, respectively).

scholarly journals Different Isolation Methods for Determination of Composition of Volatiles from Nigella damascena L. Seeds

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Anna Wajs ◽  
Radoslaw Bonikowski ◽  
Danuta Kalemba
Keyword(s):  
Volatile Oil ◽  
Quantitative Composition ◽  
Methyl Anthranilate ◽  
Qualitative And Quantitative ◽  
Lower Fatty Acid ◽  
Sesquiterpene Hydrocarbons ◽  
Volatile Organic ◽  
Conventional Solvent Extraction ◽  
Nigella Damascena

Volatile organic compounds (VOCs) from seeds of Nigella damascena L. were isolated using different techniques. The yield, as well as the qualitative and quantitative composition of the oils, was strongly influenced by the isolation method. In the hydrodistilled essential oil, the major components were the sesquiterpene hydrocarbons β-elemene (59.1%), β-selinene (12.8%) and α-selinene (12.6%). Conventional solvent extraction, followed by hydrodistillation, resulted in a volatile oil with a different composition than that of the hydrodistilled oil. The extracted oils predominantly contained sesquiterpene hydrocarbons, but also methyl anthranilate derivatives. By means of HS-SPME, it was possible to analyze not only sesquiterpenes and anthranilates, but also monoterpenes and the most volatile compounds, for example, the lower fatty acid (butyric, capronic) esters, which determine the characteristic sweet scent of N. damascena seeds. Using all testing methods, 55 compounds were identified, 40 of which are new for N. damascena seed VOCs.

scholarly journals CLINICAL AND LABORATORY ASSESSMENT THE LEVELS OF ORAL HYGIENE, TOTAL PROTEIN, HYDROGEN SULFIDE AND NITROGEN METABOLITES IN ORAL FLUID IN THE DEVELOPMENT OF INFLAMMATORY COMPLICATIONS DURING ORTHODONTIC TREATMENT OF CHILDREN

2019 ◽  
Vol 72 (5) ◽  
pp. 744-747
Author(s):  
Alisa V. Pachevska ◽  
Yurii V. Filimonov ◽  
Valerij Yu. Filimonov ◽  
Olena P. Dudik ◽  
Olena I. Popova ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Oral Cavity ◽  
Oral Hygiene ◽  
Total Protein ◽  
Orthodontic Treatment ◽  
Oral Fluid ◽  
Inflammatory Diseases ◽  
Healthy Children ◽  
Orthodontic Devices

Introduction: Orthodontic treatment often causes inflammatory diseases in the oral cavity. The aim: То іncrease the effectiveness of prevention of inflammatory complications in the provision of orthodontic care to children with dentomaxillary anomalies using nonremovable orthodontic devices on the basis of study of clinical and biochemical parameters. Materials and methods: The study was conducted among 100 patients divided into two groups: control and experimental group 2 (50 patients in each). The control group included healthy children without dento-maxillary anomalies. The second group were children aged 9-15 years with dento-maxillary anomalies which used non-removable (bracket systems) orthodontic devices. The oral fluid was collected at the beginning of the medical application of orthodontic devices (at the first day of treatment in the clinic) and after 3 and 6 months of treatment. The study included the definition of the oral hygiene degree by using the Green-Vermillion index (OHI-S), the modified Fedorov-Volodkin’s and Siness-Loe index, the determination of hydrogen sulfide and total protein levels, the content of nitrogen metabolites in the oral liquid. Results: The use of non-removable orthodontic devices led to signs of inflammation in the oral cavity. This was accompanied by a deterioration of the oral hygiene, increase the total protein, hydrogen sulfide and nitrogen metabolites levels in the oral fluid. Conclusions: Complex accounting is required of keeping oral hygiene in good condition, level determination of total protein, hydrogen sulfide and nitrogen metabolites for the prevention of the development of inflammatory diseases in the provision of orthodontic care.

Sensitive Determination of Sertraline in Commercial Drugs and Its Stability Check in Simulated Gastric Juice

2016 ◽  
Vol 99 (6) ◽  
pp. 1527-1532 ◽  
Author(s):  
Elif Seda Koçoğlu ◽  
Sezgin Bakırdere ◽  
Seyfullah Keyf
Keyword(s):  
Gastric Juice ◽  
Quantitative Measurement ◽  
Calibration Plot ◽  
Optimum Conditions ◽  
Selected Ion Monitoring ◽  
Qualitative And Quantitative ◽  
Lower Detection ◽  
Sensitive Determination ◽  
The Stability

Abstract A sensitive analytical method was developed for the determination of sertraline in commercial drug samples by using GC–MS. The selected-ion monitoring mode was used at the most sensitive m/z 274 to obtain a lower detection limit. LOD/LOQ values were obtained as 1.6/5.4 ng/mL for sertraline under the optimum conditions. The calibration plot was linear between 5.0 and 2000 ng/mL with the correlation coefficient of 0.9999. The validated method was successfully applied to three different brands of drug samples for both qualitative and quantitative measurement of sertraline. In this experiment, four replicate extractions were performed for each brand, and the results were compared to the values written on the labels of the drug brands. Spiking experiments were also performed to check the effect of the matrixes on the determination, and it was observed that there was no shift in the retention time of the analyte. In addition, simulated gastric juice experiments were performed to check the stability of sertraline in the stomach for 240 min, and it was observed that there was no change in the structure of the analyte.

scholarly journals The effect of contamination of soil by heavy metals on qualitative and quantitative composition of fungi in the rhizosphere of some forest trees

2014 ◽  
Vol 33 (1) ◽  
pp. 3-23
Author(s):  
Stefan Kowalski ◽  
Hanna Stępniewska ◽  
Zbigniew Krzan ◽  
Kazimierz Januszek
Keyword(s):  
Heavy Metals ◽  
Tree Species ◽  
Heavy Metal Contamination ◽  
Root Zone ◽  
Great Influence ◽  
Forest Tree ◽  
Quantitative Composition ◽  
Individual Tree ◽  
Qualitative And Quantitative

The effect of heavy metals in soils on the formation of fungal communities in the rhizosphere of different forest tree species was studied. Soil samples for heavy metal contamination tests and root samples for the determination of rhizospheric fungi were taken from the same root zone. The reduction of the quantitative as well as qualitative composition of fungi in the rhizosphere of individual tree species clearly correlated with the increase of soil contamination by Pb, Zn, end Cd. The following groups of fungi were distinguished: tolerant, very susceptible and relatively resistant to contamination of soil by heavy metals. Moreover a great influence of tree species on the qualitative and quantitative composition of fungi in the rhizosphere was demonstrated.

scholarly journals MODERN DIAGNOSTICS METHODS OF NON-SPECIFIC PROTECTION OF PERIODONTAL TISSUES IN RESIDENTS OF THE INDUSTRIAL REGION

2020 ◽  
pp. 18-23
Author(s):  
A.V. Samoilenko ◽  
S.V. Pavlov ◽  
I.V. Vozna
Keyword(s):  
Oral Cavity ◽  
Oral Fluid ◽  
Pathological Process ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Study Group ◽  
Periodontal Tissue ◽  
Oral Liquid ◽  
Healthy Control

The aim of the article is to study the peculiarities of antimicrobial immunity of the oral cavity of the industrial region’s inhabitants in order to optimize special preventive programs of major dental diseases. The object and research methods. 178 patients was examined from 21 to 50 years old, who turned to the University Dental Center of Zaporizhzhia State Medical University. The study group consisted of 126 patients with generalized periodontitis of the initial (8), I (32), II (68) and III (18) degree of chronic course, complicated by harmful factors of manufacturing. The comparison group consisted of 32 patients with periodontitis (5), I (10), II (11) and III (6) degrees who did not work in adverse conditions. The control group consisted of 20 relatively healthy individuals aged from 19 to 25 years without signs of generalized periodontitis. To study the factors of local immunity of the oral cavity, oral fluid was collected from each examined person, which was obtained without stimulation, spitting into sterile tubes. Then the oral fluid was centrifuged for 15 minutes at 8,000 rpm. The supernatant part of the oral liquid was poured into plastic tubes and stored at 30°C. The quantitative determination of markers in the oral fluid was performed by standard enzyme immunoassay kits according to the instructions of the "Lactoferrin-strip" ("VectorBest") manufacturers. The result was expressed in mµg / ml. The content of cathelicidin LL-37 was determined by enzyme-linked immunosorbent assay using NycultBiotechhuman LL-37 ELISA (Netherlands) firm reagent kit-manufacturers. The result was expressed in µg / ml. Statistica 13.0 licensed number JPZ804I382130ARCN10-J was used to process the results. The results of the study and their discussion. The hygienic condition in the examined patients of the study group was assessed as unsatisfactory: from 2.24 ± 0.5 to 2.99 ± 0.47 points depending on the age and length of service at the enterprise, but the PI and SBI values were increased. It was found, that the lactoferrin level in the oral fluid was higher in the all patients with periodontitis, than the same index in comparison with healthy control group and consistently increased with increasing severity of the process. In the patients’ study group, working in hazardous conditions of production, the lactoferrin content has increased in the oral fluid relative to the level of the healthy patients was more pronounced in comparison with the patients, suffering of periodontal tissue disease, but do not work in harmful manufacturing conditions. Increased lactoferrin in the oral fluid in the steelmaking workers can be considered as a means of compensation, that provides protection of the oral mucosa from colonization of microorganisms. In the steel industry workers, a statistically significant cathelicidin content decrease in the oral fluid was observed compared to healthy control patients’ group. In the clinical group of the patients without adverse factors of production, the cathelicidin’s concentration in the oral fluid was also reduced. The increasing level of lactoferrin in the oral fluid in parallel with the cathelicidin’s decrease in saliva are markers of the inflammatory phase, as well as the destructive phase of connective tissue. Conclusions. Thus, our observations have established a direct relationship between the lactoferrin’s concentration in the oral fluid and the severity of periodontal tissue diseases and the feedback between the cathelicidin’s content in the oral liquid and the activity of the pathological process. The determination of lactoferrin and cathelicidin in the oral fluid of the patients with harmful manufacturing factors allow us to identify the signs of pathological process in the oral cavity.

scholarly journals Methods for the determination of flavonoids in plant raw materials and products of their processing

2020 ◽  
Author(s):  
N.V. Alpatova ◽  
◽  
I.A. Dubrovskaya ◽  
M.V. Slobodyanik ◽  
◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Comparative Analysis ◽  
Literature Review ◽  
Raw Materials ◽  
Biologically Active ◽  
Quantitative Composition ◽  
Plant Materials ◽  
Qualitative And Quantitative ◽  
Plant Raw Materials

Flavonoids are an important component of plant materials; they have medicinal and biologically active properties. To use them as medicines, it is necessary to determine their qualitative and quantitative composition. The aim of this work is a comparative analysis of the identification and metrological characteristics of methods for the determination of flavonoids in plant raw materials and products of their processing. The paper presents a brief literature review of the main methods for the study of flavonoids. Certified and advanced techniques, such as NMR and mass spectrometry, have been discussed.

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