scholarly journals Uji Daya Hambat Ekstrak Etanol Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) terhadap Bakteri Staphylococcus aureus

2019 ◽  
Vol 4 (2) ◽  
pp. 51-59 ◽  
Author(s):  
Susi Novaryatiin ◽  
Ahmad Ramli ◽  
Syahrida Dian Ardhany
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Traditional Medicine ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Disc Diffusion ◽  
Central Kalimantan ◽  
Onion Extract ◽  
Onion Bulbs

Dayak Onion is a typical plant of Central Kalimantan. This plant has been traditionally used by the Dayak community as traditional medicine. Empirically, Dayak Onion bulbs are known to have properties to treat boils or skin diseases. The purpose of this study was to determine the ability of inhibition of Dayak Onion ethanol extract in inhibiting Staphylococcus aureus bacteria and to determine the effectiveness of the inhibition of Dayak Onion ethanol extract and concentration that could inhibit the growth of Staphylococcus aureus bacteria. This research was carried out by testing the antibacterial activity of Dayak Onion extract at levels of 1%, 5%, 10%, and 15% using the Disc Diffusion (Kirby-Bauer) method by using disc paper. The results showed that Dayak Onion ethanol extract was able to inhibit the growth of Staphylococcus aureus and effectively inhibited the growth of Staphylococcus aureus at concentrations of 1%, 5%, 10%, and 15% with inhibitory zones of 14.3 � 2.5 mm ; 16.6 � 1.7 mm; 16.2 � 2.0 mm; and 18.0 � 1.7 mm.

scholarly journals THE ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE BULBOSA (MILL.) URB.) FROM CENTRAL KALIMANTAN AGAINST ACNE-CAUSING BACTERIA

2019 ◽  
pp. 22-25
Author(s):  
SUSI NOVARYATIIN ◽  
SYAHRIDA DIAN ARDHANY
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Disc Diffusion ◽  
Central Kalimantan ◽  
Diffusion Technique ◽  
Inhibition Zones ◽  
Phytochemical Constituents

Objective: The objective of this research was to investigate the antibacterial activity of bawang dayak from Central Kalimantan against acne-causingbacteria namely Propionibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus.Methods: The preliminary phytochemical constituents were qualitatively analyzed. Antibacterial activity of bawang dayak ethanol extract wasperformed using disc-diffusion technique, with five variations of concentration of 1.25%, 2.5%, 5%, 10%, and 20%.Results: Bawang dayak extract contained flavonoids, alkaloids, saponins, and tannins. The inhibition zones of bawang dayak ethanol extract in fivevarious concentrations (1.25%, 2.5%, 5%, 10%, and 20%) were 7.0±1.3 mm, 8.5±0.6 mm, 9.9±0.3 mm, 11.2±0.1 mm, and 11.9±0.3 mm against P. acnes;18.8±3.3 mm, 21.9±3.3 mm, 20.8±0.6 mm, 22.0±0.2 mm, and 23.1±0.6 mm against S. epidermidis; and 14.3±3.1 mm, 13.5±0.9 mm, 14.7±1.5 mm,16.1±1.0 mm, and 20.1±0.6 mm against S. aureus, respectively.Conclusion: This present study showed that bawang dayak ethanol extract was active against all the tested acne-causing bacteria. The highestantibacterial activity was produced by 20% of bawang dayak ethanol extract against S. epidermidis.

scholarly journals PENGARUH PEMBENTUK FILM PVA PADA SEDIAAN MASKER GEL PEEL-OFF EKSTRAK ETANOL BAWANG DAYAK (Eleutherina palmifolia (L.) Merr) TERHADAP AKTIVITAS ANTIBAKTERI Staphylococcus aureus PENYEBAB JERAWAT

2018 ◽  
Vol 15 (2) ◽  
pp. 24
Author(s):  
Husnani Husnani ◽  
Fitri Sri Rizki
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Polyvinyl Alcohol ◽  
Ethanol Extract ◽  
Clear Zone ◽  
Onion Extract ◽  
Zone Diameter ◽  
Extract Concentration ◽  
Inhibitory Power

ABSTRACTDayak Onion (Eleutherine palmifolia) is one of the plants that can inhibit Staphylococcus aureus bacteria that causes acne. This study aims to determine the effect of polyvinyl alcohol on peel-off gel mask preparations from onion dayak ethanol extract on the antibacterial activity of Staphylococcus aureus that causes acne. Extraction using maceration method with 96% ethanol. The concentration of dayak onion extract was made at 5%, 7.5%, and 10% and the peel-off gel mask formula from dayak onion extract with variations in extract concentration were 5%, 7.5%, and 10% based on Polyvinyl Alcohol (PVA). Antibacterial activity is carried out using the disk disc method. In testing the inhibition of dayak onion extract at a concentration of 5% resulted in a clear zone of 15.5 mm, a concentration of 7.5% and 10% producing the same clear zone diameter of 17.1 mm. The peel-off gel mask in formulation I obtained a clear zone diameter of 8.7 mm, formulation II the diameter of the clear zone was 12.2 mm, and formula III the diameter of the clear zone was 15.3 mm. The results of this study indicate that on dayak onion extract has a greater inhibitory power compared to the dayak onion extract peel-off gel mask preparation. It can be concluded that the PVA in the preparation affects the amount of inhibitory power on the bacteria forming acne, namely Staphylococcus aureus.Keywords: Dayak onion extract, Staphylococcus aureus, disk discs, antibacterial inhibitory test, acne-causing bacteria, peel-off gel mask.

scholarly journals Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

2018 ◽  
Vol 1 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Dwi Latifah Sari ◽  
Sudarmi Sudarmi ◽  
Popi Patilaya
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

scholarly journals Antibacterial Activity of Ethanolic Extract of Sangkareho (Callicarpa longifolia Lam.) against Staphylococcus epidermidis

2018 ◽  
Vol 1 (2) ◽  
pp. 85-88 ◽  
Author(s):  
Susi Novaryatiin ◽  
Asma'ul Ayuriska Sari ◽  
Evi Mulyani
Keyword(s):  
Antibacterial Activity ◽  
Traditional Medicine ◽  
Staphylococcus Epidermidis ◽  
Skin Disease ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Disc Diffusion ◽  
Central Kalimantan ◽  
Inhibition Zone Diameter ◽  
Diffusion Technique

One of the plants believed as traditional medicine by the Dayak tribes in Central Kalimantan was Sangkareho (Callicarpa longifolia Lam.). This plant was usually used as traditional medicine by society in Puruk Cahu, Murung Raya Regency, Central Kalimantan and empirically believed could cure skin disease such as wound infection. This study was aimed to determine the antibacterial activity of ethanolic extract of Sangkareho (Callicarpa longifolia Lam). Antibacterial activity of ethanolic extract of Sangkareho was performed using disc diffusion technique, with four variations of concentration of 1%, 5%, 10%, and 15%. In this present study showed that ethanolic extract of Sangkareho has antibacterial activity against Staphylococcus epidermidis, wherein the resulting inhibition zone diameter was 7.3�0.8 mm, 8.3�1.2 mm, 10.5�0.5 mm, and 12.2�0.7 mm, respectively. Further research is needed to obtain an antibacterial activity of the fraction of Sangkareho.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH MAHKOTA DEWA (Phaleria macrocarpa) TERHADAP STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 9 (02) ◽  
pp. 134-139
Author(s):  
Yunilda Rosa ◽  
Masnir Alwi ◽  
Monica Julianti
Keyword(s):  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dilution Method ◽  
Phaleria Macrocarpa

Phaleria macrocarpa is one of the medicinal plants that many people use as a traditional medicinal which has many benefits, one of which is as a medicine for skin diseases. The content of flavonoid compounds in Phaleria macrocarpa believed to have potential as an antibacterial. The purpose of this study was to know the antibacterial activity of the ethanol extract of the Phaleria macrocarpa to inhibized Staphylococcus aureus bacteria. This research is an experimental study carried out by the liquid dilution method (KHM)and disk diffusion (KBM) of the extract of the Phaleria macrocarpa is divided into 6 concentrations namely 5%, 10% and 15% (KHM) and 40%, 50% and 60% (KBM). The positive control used is clindamycin and the negative control is aquadest. Data analysis uses univariate. Based on observations of the minimum inhibitory concentration of the extract of the fruit of the Phaleria macrocarpa which is at of 15%, while the minimum killed concentration is formed at of 40% with an average of 6.36 mm, and an average of zones formed on the positive control of 9.53 mm. From the results of the study, it was concluded that the extract of the fruit of the Phaleria macrocarpa can inhibit and kill the growth of Staphylococcus aureus bacteria.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK MIKROALGA Thalassiosira sp TERHADAP BAKTERI Staphylococcus aureus, Staphylococcus epidermidis dan Propionibacterium Acne

2019 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Vina Juliana Anggraeni ◽  
Titis Setyaning Wahyu ◽  
Herni Kusriani ◽  
Dewi Kurnia
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Marine Biota ◽  
Disc Diffusion ◽  
Propionibacterium Acne ◽  
X 24

AbstrakPengembangan obat dan kosmetik dari biota laut kini tengah terjadi di dunia farmasi. Mikroalga Thalassiosira sp merupakan jenis mikroalga yang memiliki kandungan senyawa-senyawa bioaktif. Beberapa penelitian sebelumnya menunjukkan bawa mikroalga memiliki aktivitas antibakteri.  Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak mikroalga Thalassiosira sp  terhadap 3 bakteri  yaitu staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne di fasa n-heksan, etil asetat dan etanol. Ketiga bakteri ini dapat menyebabkan infeksi kulit. Mikroalga Thalassiosira sp dikultivasi menggunakan medium walne dan di panen pada hari ke-6 setelah penanaman. Pemanen mikroalga dilakukan dengan teknik sentrifuga. Ektrak dilakukan dengan cara  maserasi bertingkat selama 3 x 24 jam. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram atau metode disc diffusion menurut Kirby-Bauer. Hasil ekstrak mikroalga Thalassiosira sp diperoleh paling banyak pada ekstrak etanol sebanyak 24,24%(b/b), ektrak etil asetat sebanyak 19,75%(b/b) dan paling sedikit adalah ekstrak heksan sebanyak 8,64% (b/b). Hasil uji difusi menunjukkan ekstrak n-heksan, etil asetat dan etanol mikroalga Thalassiosira sp memiliki aktivitas terhadap bakteri staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne yang ditunjukan dengan adanya zona bening. Kata kunci: mikroalga, Thalassiosira sp, antibakteri, infeksi kulit, metode difusi Abstract Development drugs and cosmetics from marine biota is now being happened in pharmacy word. Microalgae Thalassiosira sp is a type of microalgae that has a bioactive compounds. Several previous studies have shown the existence of microalgae which have antibacterial activity. This study aimed to study the antibacterial activity of extracts of microalgae Thalassiosira sp against 3 bacteria which is staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne in the n-hexane, ethyl acetate and ethanol phases. These three bacteria can cause skin infections. Microalgae Thalassiosira sp was cultivated using walne medium and harvested on the 7th day after planting. Microalgae harvesters are carried out by centrifuge techniques. The extract is done by multilevel maceration for 3 x 24 hours. Antibacterial testing was carried out by the paper diffusion method or Kirby-Bauer's disc diffusion method. The results of the crude extract of microalgae Thalassiosira sp were obtained at most in ethanol extract as much as 24.24% (w/w), extract of ethyl acetate at 19.75% (w / w) and at least hexane extract at 8.64% (w/w). The diffusion test results for n-hexane, ethyl acetate, and ethanol microalgae Thalassiosira sp extract have activity on  staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne which are supported by clear zones. Keywords: mikroalge, Thalassiosira sp, antibacteria, skin infection, diffusion method

scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 547-553
Author(s):  
Hida Ilyana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

scholarly journals Antibacterial Activities Test of Combination of Ethanolic Extract of Betel Leaves (Piper betle L.) and Basil Leaves (Ocimum basilicum L.) Against Staphylococcus aureus

2018 ◽  
Vol 9 (2) ◽  
pp. 118-126
Author(s):  
Junie Suriawati ◽  
Patimah Patimah ◽  
Siti Rahayu Rachmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Piper Betle ◽  
Betel Leaf

Betel leaf extract and basil leaf extract contain phenol compounds, safonin, flavonoids, tannins, and essential oils that act as antibacterials. Skin is very susceptible to infection or other skin diseases one of which is caused by Staphylococcus aureus bacteria. This research needs to be done to test the antibacterial activity of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The process of extraction of betel leaf and basil leaves was done with 96% ethanol solvent using maceration method. The combinations used in the sample solution were the concentration of ethanol extract of green betel leaf (0%, 15%, 30%, 45%, 60%, 75%) and basil leaf extract (0%, 15%, 30%, 45%, 60 %, 75%). Furthermore, the antibacterial activity was tested for S. aureus with well diffusion method. The data obtained were tested statistically using Anova and Duncan's advanced test. The result of statistical analysis showed difference of drag zone (p <0,05) between various concentration of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The smallest resin zone of ethanol extract of  betel leaf and ethanol extract of basil leaves is at 0% concentration (S0 and K0) and highest at 75% concentration (S5 and K5). While the smallest zone of inhibition for the combination of ethanol extract of betel leaf and ethanol extract of basil leaves at concentration S0K0, S0K1 and S0K2 is 0 mm and highest at S5K5 concentration is 31,3 mm. The higher concentration of betel leaf extract, basil leaf extract and the combination of the two extracts, the inhibition of diameter zone will be better. It can be concluded that the combination of  betel leaf extract and basil leaf extract has the ability to inhibit the growth of S. aureus better than single extract.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 246
Author(s):  
Yayuk Bulam Sarifati ◽  
Sjarif Ismail ◽  
Khemasili Kosala
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Measurement ◽  
Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of ​​inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values ​​<0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

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