scholarly journals Identifikasi Kandungan Metabolit Sekunder dan Uji Aktivitas Antioksidan Ekstrak n-Heksana Kulit Jeruk Bali (Citrus maxima Merr.).

2021 ◽  
Vol 6 (2) ◽  
pp. 185-200
Author(s):  
Elisabeth Oriana Jawa La ◽  
Repining Tiyas Sawiji ◽  
Ni Made Rai Yuliani
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Cell Damage ◽  
Chemical Compounds ◽  
The Body ◽  
Activity Test ◽  
Grapefruit Peel ◽  
Citrus Maxima ◽  
Capture Method ◽  
Layer Chromatography

Degenerative diseases triggered by antioxidants in the body are unable to neutralize the increased concentration of free radicals that can cause cell damage, so to destroy free radicals it is necessary to have antioxidants from outside the body. Grapefruit peel (Citrus Maxima Merr) is one plant that is known to have a lot of secondary metabolite content however it is not fully utilized. To analyze the content of chemical compounds and the antioxidant effect of n-hexane extract of Grapefruit peel (Citrus Maxima Merr.). Identification of chemical compounds was carried out by phytochemical screening and confirmed by the analysis of the extract thin layer chromatography profile, whereas the antioxidant activity test was carried out using the radical capture method of 2,2-diphenyl-1-picrylhydrazyl (DPPH) with Trolox as a comparison. The results of the identification of Grapefruit peel (Citrus Maxima Merr) extracts is containing secondary metabolites in the form of flavonoids, saponins, alkaloids, triterpenoids/steroids, and tannins. The results of antioxidant activity test extracts of grapefruit peel(Citrus Maxima Merr) and Trolox each showed values that: IC50 111.69 ppm and 12.143 ppm. From the results of this test, the extract of grapefruit peel (Citrus Maxima Merr) has moderate antioxidant activity when compared to Trolox.

scholarly journals Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method

2020 ◽  
Vol 3 (1) ◽  
pp. 15-21
Author(s):  
Rahmi Muthia ◽  
Muhammad Hidayatullah ◽  
Rahmi Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography ◽  
Unstable Molecule

The free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 �g/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity.

scholarly journals Uji Aktivitas Antioksidan Body Butter Ekstrak Etanol Kulit Buah Naga Merah dengan Metode DPPH

2021 ◽  
Vol 6 (2) ◽  
pp. 178-184
Author(s):  
Repining Tiyas Sawiji ◽  
Elisabeth Oriana Jawa La
Keyword(s):  
Antioxidant Activity ◽  
Cell Damage ◽  
Pharmaceutical Preparation ◽  
Ethanol Extract ◽  
The Body ◽  
Fruit Peel ◽  
Activity Test ◽  
Dragon Fruit ◽  
Ic50 Values ◽  
Hylocereus Polyrhizus

Red dragon fruit peels (Hylocereus polyrhizus) are considered important by the community. While the red dragon fruit peel contains flavonoids, betacyanin, anthocyanin, and vitamin C which have antioxidant activity so that it can ward off free radiation and prevent cell damage. Antioxidants are molecules that can prevent the oxidation of other molecules. Antioxidants can protect the skin from various free radical cell damage. The body butter is a pharmaceutical preparation in the cosmetic field that can be used for skin health care. This study aims to determine the percentage of antioxidant activity and IC50 values in the body butter ethanol extract of red dragon fruit peels. Red dragon fruit peels were extracted first using the maceration method with 96% ethanol solvent. The antioxidant activity test was measured using UV-Vis spectrophotometry. The results showed that the antioxidant activity value of formula I obtained IC50 values (56.17 μg / mL), formula II IC50 values (88.26 μg / mL), and formula III IC50 values (146.05 μg / mL). From the results obtained, the body butter formula which has the greatest activity is the formula I (AAI = 0.7). Antioxidant activity in body butter preparations is included in the group of moderate antioxidants.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT DENGAN METODE DPPH

2020 ◽  
Vol 3 (2) ◽  
pp. 405-412
Author(s):  
Weni Puspita ◽  
◽  
Dina Yuspita Sari ◽  
Ika Ristia Rahman ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Radical Oxidation ◽  
Ic50 Values ◽  
Ethanol Extracts

Buas (Premna serratifolia L.) contains secondary metabolites of flavonoids, saponins, tannins and triterpenoids/steroids, where flavonoids are known to have antioxidant properties and counteract free radicals. Antioxidants are compounds that can fight toxic hazards and reduce cell damage to the body caused by free radical oxidation. The purpose of this study was to determine the antioxidant activity of ethanol extracts of buas-buas leaves was carried out by DPPH (2,2-diphenyl-1-picrylhydrazyl), using UV-Vis spectrophotometry at wavelength 518.40 nm with various concentrations of 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. The results showed that Buas Buas leaves (Premna serratifolia L.) had an antioxidant activity with IC50 values ??of 20.66 ug / mL.

scholarly journals Uji Efek Antioksidan Formulasi Sediaan Sirup Ekstrak Etanol Kulit Buah Jeruk Bali (Citrus Maxima Merr.) Terhadap Malondialdehid Tikus Putih (Rattus Novergicus) Yang Diinduksi Karbon Tetraklorida

2021 ◽  
Vol 16 (1) ◽  
pp. 151
Author(s):  
Suryanita Suryanita ◽  
Hasma Hasma
Keyword(s):  
Free Radicals ◽  
Carbon Tetrachloride ◽  
Blood Plasma ◽  
Negative Control ◽  
The Body ◽  
Skin Extract ◽  
White Rats ◽  
Grapefruit Peel ◽  
Citrus Maxima

Free radicals are any molecule or atom that is unstable because it has one or more unpaired electrons. Free radicals that are formed in the body will produce new free radicals through a chain reaction which eventually increases in number and attacks the body's cells so that tissue damage will occur, therefore antioxidants are needed from outside to neutralize free radicals, the salute is grapefruit peel (Citrus maxima Merr.) This study aims to determine the antioxidant activity of the ethanol extract syrup of grapefruit peel against levels of Malondialdehyde (MDA) blood plasma of male white rats induced by carbon tetrachloride (CCl4). The stages of this study were the first to make a skin extract syrup. grapefruit peel containing 1% extract (F1), 2% extract (F2), 3% extract (F3), and without grapefruit peel extract (F4) as negative control; Furthermore, testing the stability of the syrup preparations before and after storage was accelerated for 12 cycles, and finally the syrup with the best stability was tested for its lipid peroxidation inhibition activity in vivo with blood plasma MDA levels in rats induced by Carbon Tetrachloride. The results showed the results of the syrup stability test. indicates that F3 has the best stability. As for the in vivo test results, F3 showed a decrease in the MDA level of blood plasma in rats which was significantly different from the negative control. The grapefruit peel significantly decreased the MDA level of the rat blood plasma with a  value p<0.05. Keywords : Free radicals, Grapefruit Peel, Antioxidant, Syrup Preparation, Malondialdehyde (MDA)

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Mundar (Garcinia forbesii King.) Menggunakan Metode DPPH (2,2-Diphenyl-1-Picrylhydrazil)

2019 ◽  
Vol 6 (1) ◽  
pp. 74 ◽  
Author(s):  
Rahmi Muthia ◽  
Revita Saputri ◽  
Sulastri Azistina Verawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Extraction Method ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Positive Control ◽  
Endemic Plants ◽  
Activity Test ◽  
Layer Chromatography

ABSTRAK             Kulit buah Mundar (G. forbesii King.) merupakan tanaman endemik yang berasal dari Kalimantan Selatan yang berpotensi memiliki aktivitas antioksidan. Penelitian ini dilakukan untuk menguji aktivitas antioksidan ekstrak etanol 30% dan 70% kulit buah Mundar (G. forbesii King.) yang diperoleh dengan metode ekstraksi sokhletasi. Pengujian aktivitas antioksidan dilakukan dengan metode DPPH (2,2-Diphenyl-1-Picrylhydrazil) secara kualititatif dan kuantitatif dengan kuersetin sebagai kontrol positif. Hasil uji aktivitas antioksidan ekstrak etanol 30% dan 70% kulit buah Mundar (G. forbesii King.) secara kualitatif menunjukkan adanya aktivitas antioksidan yang ditandai dengan noda kuning pada plat kromatografi lapis tipis (KLT) setelah disemprot dengan larutan DPPH 0,1mM. Hasil uji aktivitas antioksidan secara kuantitatif menghasilkan nilai IC50 (Inhibitory Concentration) secara berturut-turut dari ekstrak etanol 30% kulit buah Mundar (G. forbesii King.); ekstrak etanol 70% kulit buah Mundar (G. forbesii King.); dan kuersetin adalah 717,01 ppm; 534,69 ppm; dan 2,04 ppm.  Ekstrak etanol 70% kulit buah Mundar (G. forbesii King.) memiliki aktivitas antioksidan yang lebih baik dibandingkan ekstrak etanol 30% kulit buah Mundar (G. forbesii King.).  Kata kunci: Kulit buah Mundar (G. forbesii King.), antioksidan, DPPH, kromatografi lapis tipis (KLT), IC50  ABSTRACT Antioxidants are compounds that can counteract free radicals. Mundar rind (Garcinia forbesii King.) is endemic plants from South Kalimantan that has the potential antioxidant activity. This research was conducted antioxidant activity test of ethanolic extract 30% and 70% of Mundar rind (G. forbesii King.) obtained by soxhlet extraction method. Antioxidant activity test of Mundar rind (G. forbesii King.) used the DPPH (2,2-Diphenyl-1-Picrylhydrazil) method qualitatively and quantitatively with quercetin as a positive control. The results of the antioxidant activity test qualitatively of ethanolic extract 30% and 70% of Mundar rind (G. forbesii King.) showed antioxidant activity marked with a yellow spot with purple background on kromatogram after sprayed with DPPH solution 0,1mM. The results of the antioxidant activity test quantitatively obtained IC50(Inhibitory Concentration)  value respectively of ethanolic extract 30%; ethanolic extract 70%; and quercetin were 717,01 ppm; 534,69 ppm; and 2,04 ppm. The ethanolic extract 70% of Mundar rind (G. forbesii King.) had better antioxidant activity than the ethanolic extract 30% of Mundar rind (G. forbesii King.) Keywords: Mundar rind (G. Forbesii King.), antioxidant, DPPH, thin layer chromatography (TLC), IC50

scholarly journals Effects of Solo Garlic Extract in Decreasing Malondialdehyde (MDA) Levels due to E-Cigarette Exposure

2018 ◽  
Vol 7 (2) ◽  
pp. 149
Author(s):  
Aliefia Meta Duwairoh
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Normal State ◽  
Cell Damage ◽  
The Body ◽  
Garlic Extract ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats

The popularity of E-Cigarette has increased significantly over the last decade, and has potential to replace tobacco cigarettes. Aerosol which is produced by E-Cigarette, contains reactive carbonyl and free radicals, inducing oxidative stress and causing inflammation, proliferation, and cell damage. This oxidative stress can be seen through the Malondialdehyde (MDA) levels in the body, and reduced by antioxidant activity in Solo Garlic extract. The aim of this study is analyzing the differences of MDA levels in four treatment groups. 28 white rats were divided into four groups and three of them were exposured by E-Cigarette. This study showed that the highest MDA levels was found in the group which was exposured by E-Cigarette without solo garlic extract (7,179 nmol/ml). MDA levels can be reduced by antioxidant activity shown in the group which was exposured by E-Cigarette and solo garlic extract dose 0,05 gram/day (the smallest dose), amount 3,715 nmol/ml. The average of MDA levels in group which was given by the smallest dose of solo garlic extract, is lower than the control group. So, it can be concluded that using solo garlic extract less than 0,05 gram/day can restore MDA levels to a normal state (4,210 nmol/ml).

PROSPECTS FOR USING ASTRAGALUS ONOBRYCHIS AS A SOURCE OF NATURAL ANTIOXIDANTS

2021 ◽  
pp. 47-53
Author(s):  
Pozdnyakovа Т.А. ◽  
Kuleshovа Е.S. ◽  
Bubenchikov R.A.
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Chemical Interaction ◽  
Raw Materials ◽  
Natural Antioxidants ◽  
The Body ◽  
Plant Raw Materials ◽  
Total Antioxidant ◽  
Promising Source ◽  
The Relationship

The predominance of synthetic and genetically modified foods in the diet of a modern person often leads to disruption of natural metabolic processes and the accumulation of toxic free radicals in the body, which provokes the development of various diseases. Natural antioxidants from medicinal plants can help maintain health and stop the processes of peroxidation. In our opinion, Аstragalus onobrychis can become a promising source of natural antioxidants. The aim of this study was to establish the antioxidant activity in extracts from the herb Astragalus onobrychis. In order to obtain objective and reliable results during our research, we used two methods: the method of titrimetry and spectrophotometry of free radicals. The titrimetric method is based on the chemical interaction between an oxidizing agent (potassium permanganate) and natural antioxidants present in extracts from plant raw materials, since they exhibit the properties of reducing agents. The spectrophotometric method makes it possible to assess the value of antioxidant activity and the duration of the reaction by assessing the interaction of 2,2-diphenyl-1-picrylhydrazil with plant antioxidants. To establish the relationship between the antioxidant activity and the presence of phenolic compounds in the plant, the content of flavonoids and hydroxycinnamic acids in the herb Astragalus onobrychis was determined. As a result of the studies, the total antioxidant activity of extracts from the herb Astragalus onobrychis was established and it was revealed that its value depends on the type of extractant used, since this factor is associated with the content of various groups of natural antioxidants in the extraction. The data obtained indicate the prospects of using the herb Astragalus onobrychis as a source of natural antioxidants.

scholarly journals Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

2021 ◽  
Vol 332 ◽  
pp. 08005
Author(s):  
Aminah Dalimunthe ◽  
Dewi Pertiwi ◽  
Mahatir Muhammad ◽  
Denny Satria
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Litsea Cubeba ◽  
Ferric Reducing Antioxidant Power ◽  
Phenolic And Flavonoid

Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

scholarly journals A Preliminary Up-To-Date Review on Pakistani Medicinal Plants with Potential Antioxidant Activity

2020 ◽  
Vol 11 (1) ◽  
pp. 1-27
Author(s):  
Adil Hussain
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plants ◽  
Natural Antioxidants ◽  
The Body ◽  
New Drugs ◽  
Lung Damage ◽  
Present Review ◽  
Experimental Investigations ◽  
Health Related

Background: There exist natural antioxidants in plants that scavenge harmful free radicals from the body. Free radicals are species of chemical origin with an unpaired electron and play a pivotal role in combating against health-related problems like lung damage, inflammation, and cardiovascular ailments etc. Antioxidants halt the development of these free radicals called the reactive oxygen species either by chelating the trace elements or by enzymes inhibition. Objectives: The aim of present review was to collect information about Pakistani medicinally important plants with the exploration of their antioxidant potential. Methodology: Total 206 papers were looked over, which were obtained from numerous sources like; Google Scholar, Medline, PubMed, Research Gate, Science Direct, Scopus and Web of Science. Results: Overall, 93 plants representing 44 families with potential antioxidant activity reported from Pakistan have been presented in this review. Maximum number of species from Asteracea, Poaceae and Rutaceae familes were scrutinized for their potential antioxidant activity from Pakistan. Conclusion: The present review clearly designates that the presence of phytochemicals and antioxidant activity of medicinal plants from Pakistan vary with the species of the plants and material/extracts used. From this review, it is recommended to perform comprehensive experimental investigations based on toxicology and ethnopharmacology on these precious plants from Pakistan. It will be advantageous in the provision of trustworthy information to patients and determine further innovative compounds for safer and new drugs development with fewer side effects.

scholarly journals Comparative study of some bioactive compounds and their antioxidant activity of some berry types

10.5219/1132 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 515-523 ◽  
Author(s):  
Amina Aly ◽  
Rabab Maraei ◽  
Omneya Abou El-Leel
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Environmental Conditions ◽  
The Body ◽  
Harvest Time ◽  
Berry Fruits ◽  
Red Currant

Berries are wealthy in bioactive compounds like phenolic compounds and flavonoids that are deemed antioxidants and are great important to health. This research was performed to examine, recognize and compare bioactive compounds in certain types of berries and their antioxidant activity. The data show that blue berry, black berry and Egyptian black mulberry contain the highest content of most bioactive compounds such as phenolic compounds, flavonoids and tannins, while long mulberry and red currant berry have the lowest content for most of these compounds. They therefore, contain the highest value of antioxidant activity. The chemical composition of the berries varies depending on cultivar, variety, location of growth, environmental conditions and harvest time, as well as post-harvest treatments therefore the composition differed from berry fruit to another. Thus, berry fruits are very useful in nutrition to protect the body from many diseases because of its containment of these compounds, which act as free radicals scavenger that harm the body and thus rid the body of many harmful toxins.

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