scholarly journals COMPARISON OF LEAVES AND BARK MANGKOKAN (Nothopanax scutellarium) EXTRACTS AGAINST THE DEATH OF HOUSE FLIES (Musca domestica)

Author(s):  
Darmadi ◽  
Harni Sepriyani

Flies (Musca domestica) are vectors of disease that can transmit to humans. Generally, these flies controlled by using chemical insecticides. Excessive use of insecticide causes resistance and environmental pollution. The alternative way in fly control is to use natural ingredients from the extract of mangkokan stem bark and leaves (Nothopanax scutellaria). This study aims to determine the ratio of fly mortality after administration of ethanol extract of bark and methanol extract of mangkokan leaves (N. scutellarium). The method used in this study is a post-test only control group design. The average mortality rate of house flies using ethanol extract of mangkokan stem bark (N. scutellarium) was 5 for 55 minutes while methanol extract of mangkokan leaves (N. scutellarium) was 5 for 135 minutes with a p-value of 0.374 (p>0.05). It concludes that there is no significant difference in fly mortality with the ethanol extract of stem bark and methanol extract of mangkokan leaves (N. scutellarium). Result of this study shows that the ethanol extract of stem bark and mangkokan leaf extract (N. scutellarium) are equally potential natural insecticides.

2019 ◽  
Vol 2 (3) ◽  
pp. 84
Author(s):  
Rina Septiadiningsih ◽  
Euis Rohaety ◽  
Lenny Nuraeni

Sensitive period is the right time to instill the basis of the importance of counting. In addition to counting skills that must be developed also pay attention to the application of learning used. However, in the learning process, teachers often forget the importance of playing methods. If too often with giving assignments the child will feel bored, therefore what is used is the method of playing. Play method is one method that can be used by teachers to teach the ability to count children in the beginning. Based on this background, the purpose of this study was to determine the effect of the use of playing methods on initial numeracy skills. This study used the Quasi Experiment method with the Nonequevalent Control Group Design research design. The results of the pre test showed that there were no significant differences in the experimental group and the control group with p-value> 0.05, which was 0.135> 0.05 which was tested by the two average test results. While the post-test results showed that the initial numeracy ability after the application of the playing method experienced a significant difference with the p-value <0.05, that is with the results of 0.0001 with the Mann-Whitney test. This method of playing can be used as a learning suggestion for educators to improve their ability to have an early start.


DENTA ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Dwi Andriani ◽  
Diana Soesilo ◽  
Ghora Setyawan

<p><strong>Background:</strong> <em>Corticosteroid has a strong anti-inflammatory effect, unfortunately long-term usege of corticosteroid drugs can suppressed immune system. This condition makes Candida albicans as an opportunistic phatogen caused Oral Candidiasis. Macrophages have an important role in the first defense against infection. Methanol extract of A.ilicifolius leaf  has anti-fungal, anti-inflammatory compounds to fight the growth of Candida albicans. <strong>Purpose:</strong> To determine the effect of methanol extract of A.ilicifolius leaf theraphy to the amount of macrophages in the healing pricess of oral candidiasis models with immunosuppressed conditions. <strong>Method:</strong> </em><em>This study was true experimental with post test only control group design. Thirty-six strain wistar, 3 months old, and 250 grams of weight which were given dexamethason 0.5 mg and tetracycline 0.5mg for 7days, after Candida albican(ATCC10231) was induced for 2 weeks (3 times/week). Rats were divided into 6 groups: untreated(K-), nystatin(K+), A.ilicifolius 8%(P1), A.ilicifolius 12%(P2), A.ilicifolius 16%(P3), A.ilicifolius 20%(P4 ) all groups were treated for 2 weeks. After being treated, the rats tongue biopsied to examine the macrophages with Haematoxylin-eosin staining and observed with microscope (400x magnification). Data analyzed statistically by One-way ANOVA. <strong>Results:</strong> the amount of macrophages at K+(47.60±4.16), P4(40.20±3.56), P3(36.20±1.92), P2(17.00±2.45) were greater than K-(15.60±4.56), P1(14.60±2.70). there are no significant difference between group K- with P1 and P2, P3 with P4. There are significant difference between group K- with K+, K- with P3 and P4. K+ with P1, P2, P3 and P4. Group P1 with P3 and P4. Group P2 with P3 and P4. </em></p><strong><em>Conclusion:</em></strong><em> A.ilicifolius extract can increase the amount of macrophages in oral candidiasis immunosuppression models</em>


2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Nuriah Arma ◽  
Yanwirasti Ariadi ◽  
Lisma Evareny

AbstrakBayi baru lahir memiliki 80 ml darah dari plasenta pada 1 menit setelah kelahiran dan 100 ml pada 3 menit setelah lahir, volume ini akan memasok 40-50 mg/kg ekstra besi untuk memiliki 75 mg/kg besi tubuh bayi yang cukup bulan yang dapat mencegah kekurangan zat besi pada tahun pertama kehidupan. Oleh karena itu pemotongan tali pusat yang terlalu cepat setelah persalinan akan mengurangi kandungan besi sekitar 15-30%, sedangkan bila ditunda 3 menit dapat menambah volume sel darah merah sekitar 58%. Tujuan penelitian ini adalah untuk membuktikan adanya perbedaan kadar Hb (hemoglobin) dan Ht (hematokrit) akibat perbedaan waktu penjepitan tali pusat. Penelitian ini dilakukan di tempat Bidan Praktek Mandiri Kota Padang. Jenis penelitian eksperimental dengan post-test only controll group design. Pengambilan sampel secara consecutive sampling sampai tercapai jumlah 36 bayi yang terbagi atas 18 bayi baru lahir pada tiap kelompok. Data dianalisis dengan uji t. Hasil penelitian didapatkan rerata kadar Hb dan Ht pada kelompok penjepitan 3 menit setelah lahir lebih tinggi daripada penjepitan 1 menit. Terdapat perbedaan kadar Hb yang bermakna antara kelompok penjepitan 3 menit setelah lahir daripada 1 menit dengan nilai P=0,004.  Ada terdapat perbedaan Ht yang bermakna antara kelompok penjepitan 3 menit dan 1 menit dengan nilai P=0,001. Kesimpulan penelitian ini ialah kadar Hb dan Ht lebih baik pada penjepitan tali pusat ditunda 3 menit setelah lahir dibandingkan dengan penjepitan 1 menit setelah lahir.Kata kunci: hemoglobin, hematokrit, penjepitan tali pusat AbstractThe newborns contain 80 ml of blood from the placenta in 1 minute after birth and 100 ml in 3 minute after birth. This volume supply 40 to 50 mg/kg of extra iron to have 75 mg/kg of body iron baby full-term that can prevent iron deficiency in the first year of life. Therefore, cutting the umbilical cord too soon after birth will reduce the iron of content about 15 to 30%, whereas when delayed 3 minute its can increase the volume red blood cells about 58%. The objective of this study was to prove the difference of hemoglobin and hematocrit level between 3 minutes and 1 minute cord clamping.  Experimental research was conducted with post-test only control group design of 36 newborns. Data were analyzed by t-test. The result of this study showed that the mean of hemoglobin and hematocrit level in 3 minutes of clamp are higher  than 1 minute of clamp.There was a significant difference of hemoglobin level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.004. There was also a significant difference of hematocrit level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.001. The conclusion is the hemoglobin and hematocrit level are better in cord clamping delayed in 3 minutes after birth than clamping in 1 minute after birth. Keywords: hemoglobin, hematocrit, cord clamping


2019 ◽  
Vol 2 (3) ◽  
pp. 65
Author(s):  
Elsa Nurfuady ◽  
Heris Hendriana ◽  
Ghina Wulansuci

Basically humans have had creative potential since he was created. With the potential of its natural creativity, it is expected that early on, children will need activities related to creative ideas through coaching so that children can develop their creative potential optimally. For that we need a learning method that can improve children's creativity, one of which is the experimental method. Through the experimental method children can be trained in developing creativity. The purpose of this study is to examine the achievement of children's creativity in using experimental methods. This study used a quasi-experimental method with Nonequevalent Control Group Design research design using two classes consisting of B1 experimental group and B2 control class at Al-Masruroh Kindergarten. Based on data processing and research results obtained the results of the pre-test showed that there were no significant differences between the experimental group and the control group (TK Al-Masruroh) with p-value> 0.05 which was 0.145> 0.05, while the post test results showed creativity after the application of the experimental method there is a significant difference with the value of pvalue <0.05 which is the result of 0.000 <0.05. Based on the results of this study, the researcher recommends to child educators that the experimental method can be used as one of the learning methods to improve children's creativity.


Author(s):  
Berliana Hamidah ◽  
Ashon Sa'adi ◽  
Rina Yudiwati

The aim of this study is to learn about the choice of storage temperature for human sperm cells after sperm purification using somatic cell lysis buffer (SCLB) before sperm small ribonucleic acid (RNA) isolation and analysis. This study was true laboratory experiment using the post-test only control group design. The samples were 13 fresh human semen that has been purified using SCLB. The sperm cells then diluted and divided into four aliquots with different treatment. First aliquot that served as a control group was immediately purified while the last three aliquots were stored for 7 days at different temperature: 4oC, -20o, and -80oC. The small RNA yields between each group then compared after the small RNA isolation and measured using microvolume spectrophotometer. The small RNA yields of control group was 49.8 (5.33-522.46) ng/106 sperm cells. It wasn’t show any significant difference with the other groups of storage temperature: 4oC, -20o, and -80oC [41.09 (7.03-1448.31); 65.95 (7.99-301.16); 76.42 (10.45-434.25); p value 0.314] with p value > α (α = 5%). This condition shows that human sperm cells after sperm purification using SCLB can be stored at 4oC, -20o, or -80oC temperatures depends on each laboratory facilities.


2019 ◽  
Vol 17 (2) ◽  
pp. 176-183
Author(s):  
Kuntum Ekawati Nurdin ◽  
Loisa Ratna Yuvita Olla ◽  
Stefany Febiand Feoh ◽  
Anita Dwi Putri Galla ◽  
Khusnul Dwi Istnaini ◽  
...  

One of the plants that can be used as larvicides is sweet orange (Citrus sinensis). Sweet orange rind containing saponins, tannins, flavonoids, and triterpenoids have a characteristic of aromatic odor and bitter taste which contain 96% essential oils containing limonene, glucoside, hesperidium, and resin that can function as biolarvasides. The Regency of the Middle East South is one of the areas in Indonesia which is known as the center of sweet orange. Soe sweet orange fruit has a distinctive color, aroma, and taste compared to other oranges in Indonesia. The objectives of this study are to discover the effectiveness of the orange rind extract in killing the Aedes aegypti larvae and to identify the minimum concentration of the extract of the sweet, dry rind in killing the larvae. This type of research is experimental post-test only control group design. This research was conducted at the Health Analyst laboratory of Kupang Poltekkes (Health Polytechnic) in January 2019. The third (III) instar larvae were placed in 5 vials, each containing 15 larvae. The total number of samples needed was 375 larvae. It was mixed with 15 ml of Soe sweet orange rind with a concentration of 0.075%, 0.1%, 0.25%, 0.50%, 0.75%. One-Way Anova test results obtained sig p-value=0,000 (p<0.05), meaning that there is an influence of sweet orange rind ethanol extract on the death of larvae or orange rind extract is effective as a natural insecticide. Significant values ​​were obtained for all concentrations p=0.008 (p> 0.05) meaning that there was an average difference of each concentration. The conclusion of the study shows that the ethanol extract of sweet orange rind (Citrus sinensis L.) is effective in killing Aedes aegypti mosquito larvae with a minimum concentration of ethanol extract sweet orange rind (Citrus sinensis L.) which can kill 0.075% of larvae.


Author(s):  
Vina Yuliana ◽  
Yamtana Yamtana ◽  
Abdul Hadi Kadarusno

Abstract: Spraying application leaf squeeze kamboja (plumeria acuminata) to death fly house (musca domestica). Housefly (Musca domestica) is a vector for transmission of various types of mechanically transmitted diseases such as diarrhea, dysentery, cholera, typhoid, salmonellosis and viral gastrointestinal diseases. One of the control flies using environmentally friendly plant-based insecticides.  Researchers used insecticides in the form of freshly squeezed vegetable leaf with frangipani (Plumeria acuminata). The objective was to knowing the influence of leaf squeeze spraying frangipani with a concentration of 40 percent, 50 percentand and 60 percent of the death house fly. This study uses experimental design With Post Test Only Control Group Design. The object of this study is the 480 flies were obtained from the TPS Market Tlagareja. This research was carried out by spraying the leaf squeeze frangipani at a concentration of 40%, 50% and 60%. Counting the number of deaths house fly performed after 24 hours. The results of this research are there meaningful influence of sraying the frangipani leaf squeeze againts the death house flies and the value of sig 0,000. Concentration of 40%, 50%, and 60% respectively can be deadly flies an average of 5 tails, 10 tails and 15 tails. The most effective concentration was 60% obtained from the LSD test with significance level of 0.000 and Different Mean values (IJ) is the largest 10.00000. The conclusion is an effect spraying frangipani leaf squeeze with a concentration of 40%, 50%, and 60% of the death house fly. The most effective concentration to kill flies is at 60%, with an average mortality of flies 15 tails. Keywords: frangipani leaf, house flies, insecticide plant


2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ani Yuanita ◽  
Ngadino . ◽  
Suprijandani .

Flies is one type of Arthropod insect as a vector and carrier of disease. Control of flies by using chemical insecticides continuously results in resistance to flies and can pollute the environment. Another alternative that can be used is a plant insecticide that is derived from breadfruit leaf plants. Phytochemical results of breadfruit leaves have 1.88% Flavonoids, Saponins 2.26%, and Tanins 1.05%. The purpose of this study was to determine the potential of breadfruit leaf filtrate as a bioinsecticide of house flies (Musca domestica).This type of research uses pure experiments with a post test only control group design with a concentration of 2.5%, 5%, 7.5%, control and 5 replications. The number of house flies used was 375. Data were analyzed using the Kruskal Wallis test.The results showed p 0.05 which means that there were differences in the average death of house flies by using various concentrations of each treatment. Probit test obtained Lethal Concentration (LC50) results at the first hour ie at a concentration of 5.189%, the 12th hour at a concentration of 3.930% and the 24th hour showed a concentration of 3.086%.Suggestions for other researchers can do the same research by comparing with other plants that contain active compounds that have the potential to kill house flies (Musca domestica). Other plants that have higher active compound content can be used as a recommendation for bio-degradable plant-based insecticides.


Author(s):  
Bambang Widayanto ◽  
Alex Pangkahila ◽  
Muh Irfan ◽  
Ida Bagus Ngurah ◽  
I Putu Adiartha Griadhi ◽  
...  

Stroke is a syndrome caused by circulatory disorders of the brain (CVA) with acute onset, accompanied by clinical manifestations in the form of neurological deficit and not as a result of tumor, trauma or infection of the central nervous system. Perfomance Mobility impairment is the consequences caused by stroke. The purpose of this study was to compare a Active One Leg Standing and Contactual Hand Orientating Response (CHOR) Exercise in order to improve performance mobility instroke patients. This research used experimental methods with pre-test and post-test control group design. The number of samples per group is 7 people. Group I was gaveActive One Leg Standing Exercise, group II was gaveContactual Hand Orientating Response (CHOR) Exercise. Each group is given an exercise with an hour duration, 3 times a week for 6 weeks. Mobility perfomance measured using POMA-TinettiTest. It was concluded that Active One Leg Standing and contactual Hand orientating Response (CHOR) exercise can improve perfomance mobility in post-stroke patients. There is a significant difference in perfomance mobility level between Active One Leg Standing and contactual Hand orientating Response (CHOR) exercise.Result: (1) The result of t-test related showed mean ± SB pre test (15.57 ± 2.29) and post test (21.42 ± 2.50) with p value <0.05. (2) The result of t-test related showed mean ± SB pre test (13.85 ± 3.93) and post test (18.14 ± 3.89) with p value <0.05. (3) The result of comparation test showed the mean ± SB group I (6.85 ± 1.06) and group II (4.28 ± 0.75) with p value <0.05.


Author(s):  
Gracia Satyawestri Pribadi ◽  
Marlik Marlik

Diarrhea is still a problem for public health in developing countries like Indonesia, with house flies (Musca domestica) as vectors. One way to control flies that are safe and natural is to use plant repellents including basil leaves. This study aimed to analyze the potential of basil leaf extract as a repellent, using a post test only with control group design, with 25 house flies (Musca domestica) as objects with 6 replications. Basil leaf extract was mixed into wax with a concentration of 0% (control), 17.5%, 20%, and 22.5% which was exposed to house flies within 1 hour. The results showed that basil leaf extract can be used as a repellent for house flies. Based on the percentage of aromatic waxing power, it was known that the higher the concentration of basil leaf extract, the higher the repelling power produced. Keywords: basil leaves, repellent, house flies ABSTRAK Diare masih menjadi masalah bagi kesehatan masyarakat di negara berkembang seperti di Indonesia, dengan lalat rumah (Musca domestica) sebagai vektor. Salah satu cara pengendalian lalat yang aman dan alami adalah menggunakan repellent tumbuhan antara lain daun kemangi. Penelitian ini bertujuan untuk menganalisis potensi ekstrak daun kemangi sebagai repellent, menggunakan rancangan post test only with control group, dengan lalat rumah (Musca domestica) sejumlah 25 ekor sebagai obyek dengan 6 kali replikasi. Ekstrak daun kemangi dicampurkan ke dalam lilin dengan konsentrasi 0% (kontrol), 17.5%, 20%, dan 22.5% yang dipaparkan pada lalat rumah dalam waktu 1 jam. Hasil penelitian menunjukkan bahwa ekstrak daun kemangi dapat digunakan sebagai repellent untuk lalat rumah. Berdasarkan persentase daya tolak lilin aromatik, diketahui bahwa semakin tinggi konsentrasi ekstrak daun kemangi, maka akan semakin tinggi daya tolak yang dihasilkan. Kata kunci: daun kemangi, repellent, lalat rumah


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