scholarly journals Human Sperm Cells After Purification Using SCLB Can Be Stored at 4o, -20o, or -80oC Before Small RNA Isolation

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Berliana Hamidah ◽  
Ashon Sa'adi ◽  
Rina Yudiwati
Keyword(s):  
Small Rna ◽  
Storage Temperature ◽  
Rna Isolation ◽  
Human Sperm ◽  
Control Group ◽  
P Value ◽  
Sperm Cells ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test

The aim of this study is to learn about the choice of storage temperature for human sperm cells after sperm purification using somatic cell lysis buffer (SCLB) before sperm small ribonucleic acid (RNA) isolation and analysis. This study was true laboratory experiment using the post-test only control group design. The samples were 13 fresh human semen that has been purified using SCLB. The sperm cells then diluted and divided into four aliquots with different treatment. First aliquot that served as a control group was immediately purified while the last three aliquots were stored for 7 days at different temperature: 4oC, -20o, and -80oC. The small RNA yields between each group then compared after the small RNA isolation and measured using microvolume spectrophotometer. The small RNA yields of control group was 49.8 (5.33-522.46) ng/106 sperm cells. It wasn’t show any significant difference with the other groups of storage temperature: 4oC, -20o, and -80oC [41.09 (7.03-1448.31); 65.95 (7.99-301.16); 76.42 (10.45-434.25); p value 0.314] with p value > α (α = 5%). This condition shows that human sperm cells after sperm purification using SCLB can be stored at 4oC, -20o, or -80oC temperatures depends on each laboratory facilities.

scholarly journals PENERAPAN METODEBERMAIN ANAK USIA DINI PADA KELOMPOK B UNTUK MENINGKATKAN KEMAMPUAN BERHITUNG PERMULAAN DI TK AL-FALAH

2019 ◽  
Vol 2 (3) ◽  
pp. 84
Author(s):  
Rina Septiadiningsih ◽  
Euis Rohaety ◽  
Lenny Nuraeni
Keyword(s):  
Design Research ◽  
Sensitive Period ◽  
Control Group ◽  
P Value ◽  
Test Results ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
The Right ◽  
In The Beginning

Sensitive period is the right time to instill the basis of the importance of counting. In addition to counting skills that must be developed also pay attention to the application of learning used. However, in the learning process, teachers often forget the importance of playing methods. If too often with giving assignments the child will feel bored, therefore what is used is the method of playing. Play method is one method that can be used by teachers to teach the ability to count children in the beginning. Based on this background, the purpose of this study was to determine the effect of the use of playing methods on initial numeracy skills. This study used the Quasi Experiment method with the Nonequevalent Control Group Design research design. The results of the pre test showed that there were no significant differences in the experimental group and the control group with p-value> 0.05, which was 0.135> 0.05 which was tested by the two average test results. While the post-test results showed that the initial numeracy ability after the application of the playing method experienced a significant difference with the p-value <0.05, that is with the results of 0.0001 with the Mann-Whitney test. This method of playing can be used as a learning suggestion for educators to improve their ability to have an early start.

scholarly journals Perbedaan Kadar Hemoglobin dan Hematokrit Bayi Baru Lahir Akibat Perbedaan Waktu Penjepitan Tali Pusat

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Nuriah Arma ◽  
Yanwirasti Ariadi ◽  
Lisma Evareny
Keyword(s):  
Control Group ◽  
P Value ◽  
Hematocrit Level ◽  
Control Group Design ◽  
Group Design ◽  
Cord Clamping ◽  
Significant Difference ◽  
Post Test ◽  
The Difference ◽  
First Year Of Life

AbstrakBayi baru lahir memiliki 80 ml darah dari plasenta pada 1 menit setelah kelahiran dan 100 ml pada 3 menit setelah lahir, volume ini akan memasok 40-50 mg/kg ekstra besi untuk memiliki 75 mg/kg besi tubuh bayi yang cukup bulan yang dapat mencegah kekurangan zat besi pada tahun pertama kehidupan. Oleh karena itu pemotongan tali pusat yang terlalu cepat setelah persalinan akan mengurangi kandungan besi sekitar 15-30%, sedangkan bila ditunda 3 menit dapat menambah volume sel darah merah sekitar 58%. Tujuan penelitian ini adalah untuk membuktikan adanya perbedaan kadar Hb (hemoglobin) dan Ht (hematokrit) akibat perbedaan waktu penjepitan tali pusat. Penelitian ini dilakukan di tempat Bidan Praktek Mandiri Kota Padang. Jenis penelitian eksperimental dengan post-test only controll group design. Pengambilan sampel secara consecutive sampling sampai tercapai jumlah 36 bayi yang terbagi atas 18 bayi baru lahir pada tiap kelompok. Data dianalisis dengan uji t. Hasil penelitian didapatkan rerata kadar Hb dan Ht pada kelompok penjepitan 3 menit setelah lahir lebih tinggi daripada penjepitan 1 menit. Terdapat perbedaan kadar Hb yang bermakna antara kelompok penjepitan 3 menit setelah lahir daripada 1 menit dengan nilai P=0,004.  Ada terdapat perbedaan Ht yang bermakna antara kelompok penjepitan 3 menit dan 1 menit dengan nilai P=0,001. Kesimpulan penelitian ini ialah kadar Hb dan Ht lebih baik pada penjepitan tali pusat ditunda 3 menit setelah lahir dibandingkan dengan penjepitan 1 menit setelah lahir.Kata kunci: hemoglobin, hematokrit, penjepitan tali pusat AbstractThe newborns contain 80 ml of blood from the placenta in 1 minute after birth and 100 ml in 3 minute after birth. This volume supply 40 to 50 mg/kg of extra iron to have 75 mg/kg of body iron baby full-term that can prevent iron deficiency in the first year of life. Therefore, cutting the umbilical cord too soon after birth will reduce the iron of content about 15 to 30%, whereas when delayed 3 minute its can increase the volume red blood cells about 58%. The objective of this study was to prove the difference of hemoglobin and hematocrit level between 3 minutes and 1 minute cord clamping.  Experimental research was conducted with post-test only control group design of 36 newborns. Data were analyzed by t-test. The result of this study showed that the mean of hemoglobin and hematocrit level in 3 minutes of clamp are higher  than 1 minute of clamp.There was a significant difference of hemoglobin level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.004. There was also a significant difference of hematocrit level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.001. The conclusion is the hemoglobin and hematocrit level are better in cord clamping delayed in 3 minutes after birth than clamping in 1 minute after birth. Keywords: hemoglobin, hematocrit, cord clamping

scholarly journals PENERAPAN METODE EKSPERIMEN UNTUK MENINGKATKAN KREATIVITAS PADA ANAK USIA DINI

2019 ◽  
Vol 2 (3) ◽  
pp. 65
Author(s):  
Elsa Nurfuady ◽  
Heris Hendriana ◽  
Ghina Wulansuci
Keyword(s):  
Experimental Method ◽  
Design Research ◽  
Control Group ◽  
P Value ◽  
Creative Potential ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Quasi Experimental ◽  
Experimental Group

Basically humans have had creative potential since he was created. With the potential of its natural creativity, it is expected that early on, children will need activities related to creative ideas through coaching so that children can develop their creative potential optimally. For that we need a learning method that can improve children's creativity, one of which is the experimental method. Through the experimental method children can be trained in developing creativity. The purpose of this study is to examine the achievement of children's creativity in using experimental methods. This study used a quasi-experimental method with Nonequevalent Control Group Design research design using two classes consisting of B1 experimental group and B2 control class at Al-Masruroh Kindergarten. Based on data processing and research results obtained the results of the pre-test showed that there were no significant differences between the experimental group and the control group (TK Al-Masruroh) with p-value> 0.05 which was 0.145> 0.05, while the post test results showed creativity after the application of the experimental method there is a significant difference with the value of pvalue <0.05 which is the result of 0.000 <0.05. Based on the results of this study, the researcher recommends to child educators that the experimental method can be used as one of the learning methods to improve children's creativity.

scholarly journals ACTIVE ONE LEG STANDING EXERCISE LEBIH EFEKTIF DARIPADA CONTACTUAL HAND ORIENTATING RESPONSE (CHOR)EXERCISE UNTUK MENINGKATKAN KEMAMPUAN MOBILITAS PASIEN PASCA STROKE

2017 ◽  
Author(s):  
Bambang Widayanto ◽  
Alex Pangkahila ◽  
Muh Irfan ◽  
Ida Bagus Ngurah ◽  
I Putu Adiartha Griadhi ◽  
...  
Keyword(s):  
Clinical Manifestations ◽  
T Test ◽  
Control Group ◽  
P Value ◽  
Mobility Impairment ◽  
Control Group Design ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Stroke is a syndrome caused by circulatory disorders of the brain (CVA) with acute onset, accompanied by clinical manifestations in the form of neurological deficit and not as a result of tumor, trauma or infection of the central nervous system. Perfomance Mobility impairment is the consequences caused by stroke. The purpose of this study was to compare a Active One Leg Standing and Contactual Hand Orientating Response (CHOR) Exercise in order to improve performance mobility instroke patients. This research used experimental methods with pre-test and post-test control group design. The number of samples per group is 7 people. Group I was gaveActive One Leg Standing Exercise, group II was gaveContactual Hand Orientating Response (CHOR) Exercise. Each group is given an exercise with an hour duration, 3 times a week for 6 weeks. Mobility perfomance measured using POMA-TinettiTest. It was concluded that Active One Leg Standing and contactual Hand orientating Response (CHOR) exercise can improve perfomance mobility in post-stroke patients. There is a significant difference in perfomance mobility level between Active One Leg Standing and contactual Hand orientating Response (CHOR) exercise.Result: (1) The result of t-test related showed mean ± SB pre test (15.57 ± 2.29) and post test (21.42 ± 2.50) with p value <0.05. (2) The result of t-test related showed mean ± SB pre test (13.85 ± 3.93) and post test (18.14 ± 3.89) with p value <0.05. (3) The result of comparation test showed the mean ± SB group I (6.85 ± 1.06) and group II (4.28 ± 0.75) with p value <0.05.

scholarly journals Pemberian Ekstrak Cacao (Theobroma Cacao L) Pada Model Huvecs Preeklampsia Terhadap Kadar Interleukin 8

2019 ◽  
Vol 13 (2) ◽  
pp. 93
Author(s):  
Budi Rahayu ◽  
Liberty Barokah
Keyword(s):  
Multiple Comparisons ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Design ◽  
Tnf Α ◽  
Significant Difference ◽  
Post Test ◽  
Theobroma Cacao L

Preeklampsia dan eklampsia adalah penyakit pada kehamilan yang menjadi salah satu penyumbang angka kematian Ibu khususnya di Indonesia. Tahapan pertama dari preeklampsia ini adalah terjadi abnormalitas dalam remodelling vaskular trofoblastik mengakibatkan disfungsi endotel, tahap kedua pelepasan faktor plasenta kedalam sirkulasi darah seperti soluble fms-like tyrosine kinase (sFlt-1), TNF-α, IL-6, IL-8, angiotensin II tipe 1 reseptor antibodies (AT1-AA), dan thromboxan (TX) menyebabkan respon inflamasi dan peningkatan aktivasi sel endotel. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak kakao terhadap kadal IL-8 pada model HUVECs Preeklampsia. Penelitian true experimental dengan pendekatan yang digunakan  post test only control group design yang dikerjakan dilaboratorium dengan invitro. Kultur HUVECs dibagi menjadi 5 kelompok masing-masing dilakukan replikasi 5x. Pengukuran kadar IL-8 menggunakan ELISA (Enzyme-Linked Immunosorbent Assay). Analisis data yang digunakan pada penelitian ini adalah dengan  uji Anova one way pada kadar IL-8, selanjutnya dilakukan uji perbandingan berganda (Multiple Comparisons) dengan uji Beda Nyata Terkecil/BNT (Least Significant Difference/LSD) Hasil penelitian pemberian ekstrak kakao pada kelompok kontrol positif dan 3 dosis kelompok perlakuan ternyata dapat menurunkan secara bermakna (p-value 0.000< α 0.05, Anova One way) kadar IL-8.

scholarly journals Pengaruh Teh Hijau Terhadap Kadar Gula Darah dan MDA Serum Mencit Diabetes

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Rizka Karima Husfa
Keyword(s):  
Blood Glucose ◽  
Green Tea ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
The Mean ◽  
Test Control ◽  
Diabetes Melitus

Abstrak Diabetes melitus merupakan penyakit metabolik kronik yang ditandai dengan hiperglikemia yang jika terjadi secara terus-menerus akan menghasilkan radikal bebas berlebihan yang berperan dalam komplikasi diabetes. Teh hijau memiliki banyak kandungan katekin yang berperan sebagai antihiperglikemik dan antioksidan untuk mencegah komplikasi diabetes. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian teh hijau terhadap kadar gula darah dan MDA serum mencit yang diinduksi aloksan. Penelitian ini merupakan true experimental dengan randomized post-test control group design. Sampel terdiri dari 35 ekor mencit yang dibagi menjadi lima kelompok, yaitu kelompok kontrol negatif (K-), kontrol positif (K+), perlakuan 1 (P1), perlakuan 2 (P2), dan perlakuan 3 (P3). Kelompok K- adalah kelompok normal yang hanya diberikan diet standar, kelompok K+ diinduksi aloksan saja, kelompok P1, P2, dan P3 diinduksi aloksan dan diberi infusa teh hijau 1%, 2%, dan 4% selama 15 hari. Rata-rata kadar gula darah kelompok K-, K+, P1, P2, dan P3 adalah 73,14 mg/dl, 210 mg/dl, 164,57 mg/dl, 152,57 mg/dl, dan 135,83 mg/dl. Terdapat perbedaan signifikan antara kelompok P1, P2, dan P3 dengan kelompok K+ dengan nilai p=0,001. Rata-rata kadar MDA serum kelompok K-, K+, P1, P2, dan P3 adalah 2,54 nmol/mg, 4,04 nmol/mg, 3,05 nmol/mg, 2,87 nmol/mg, dan 2,47 nmol/mg. Terdapat perbedaan signifikan antara kelompok P1, P2, dan P3 dengan kelompok K+ dengan nilai p=0,001. Kesimpulan hasil penelitian adalah pemberian infusa teh hijau berpengaruh terhadap penurunan kadar gula darah dan MDA serum mencit yang diinduksi aloksan. Kata kunci: teh hijau, Camellia sinensis, diabetes melitus, gula darah, MDA serum, aloksan.   Abstract Diabetes mellitus is a chronic metabolic disease, characterized by hyperglycemia which if occurs continously will produce excessive free radical that have role in diabetes complication. Green tea has a lot of cathecin which has role as antihyperglycemia and antioxidant to prevent diabetes complication. The aim of this research is to determine the effect of green tea on blood glucose level and serum MDA level in alloxan-induced mice. This research was a true experiment with randomized post-test control group design. The sample consisted of 35 mice divided into five groups, negative control (K-), positive control (K+), treatment 1 (P1), treatment 2 (P2), and treatment 3 (P3). The K- group was the normal group, given standard diet only, the K+ group was induced alloxan only, the P1, P2, and P3 groups were induced alloxan and given 1%, 2%, and 4% green tea infusion for 15 days. The mean of blood glucose level on K-, K+, P1, P2, and P3 group were 73,14 mg/dl, 210 mg/dl, 164,57 mg/dl, 152,57 mg/dl, and 135,83 mg/dl. There were significant difference between P1, P2, and P3 group with K+ group with p value=0,001. The mean of serum MDA level on K-, K+, P1, P2, and P3 group were 2,54 nmol/mg, 4,04 nmol/mg, 3,05 nmol/mg, 2,87 nmol/mg, and 2,47 nmol/mg. There were significant difference between P1, P2, and P3 group with K+ group with p value=0,001.The conclusion is green tea infusion can reduce the level of blood glucose and serum MDA in alloxan-induced mice. Keywords: green tea, Camellia sinensis, diabetes mellitus, blood glucose, serum MDA, alloxan

scholarly journals COMPARISON OF LEAVES AND BARK MANGKOKAN (Nothopanax scutellarium) EXTRACTS AGAINST THE DEATH OF HOUSE FLIES (Musca domestica)

2020 ◽  
Vol 2 (2) ◽  
pp. 68-75
Author(s):  
Darmadi ◽  
Harni Sepriyani
Keyword(s):  
Musca Domestica ◽  
Methanol Extract ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
House Flies ◽  
Significant Difference ◽  
Post Test

Flies (Musca domestica) are vectors of disease that can transmit to humans. Generally, these flies controlled by using chemical insecticides. Excessive use of insecticide causes resistance and environmental pollution. The alternative way in fly control is to use natural ingredients from the extract of mangkokan stem bark and leaves (Nothopanax scutellaria). This study aims to determine the ratio of fly mortality after administration of ethanol extract of bark and methanol extract of mangkokan leaves (N. scutellarium). The method used in this study is a post-test only control group design. The average mortality rate of house flies using ethanol extract of mangkokan stem bark (N. scutellarium) was 5 for 55 minutes while methanol extract of mangkokan leaves (N. scutellarium) was 5 for 135 minutes with a p-value of 0.374 (p>0.05). It concludes that there is no significant difference in fly mortality with the ethanol extract of stem bark and methanol extract of mangkokan leaves (N. scutellarium). Result of this study shows that the ethanol extract of stem bark and mangkokan leaf extract (N. scutellarium) are equally potential natural insecticides.

PENINGKATAN KEMAMPUAN PEMAHAMAN DAN KREATIVITAS MATEMATIS SISWA DENGAN MENGGUNAKAN MODEL PENCAPAIAN KONSEP

2013 ◽  
Vol 19 (1) ◽  
pp. 52
Author(s):  
Adi Suarman Situmorang
Keyword(s):  
Random Sampling ◽  
Control Group ◽  
P Value ◽  
Design Data ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Test Control

Abstract Tujuan penelitian bertujuan untuk mengetahui peningkatan kemampuan kreativitas matematika siswa yang diajar dengan model pencapaian konsep lebih tinggi dari siswa yang diajar dengan pembelajaran konvensional. Mengetahui interaksi antara model pembelajaran pencapaian konsep dengan tingkat kemampuan matematika siswa terhadap peningkatan kemampuan kreativitas matematika siswa. Penelitian ini dilaksanakan di SMA Negeri 5 Medan dengan jumlah sampel sebanyak 80 siswa dari 364 siswa SMA kelas X melalui teknik random sampling, Penelitian ini merupakan suatu studi eksperimen dengan desain penelitian pre-test-post-test control group design. Data diperoleh melalui nilai semester untuk kemampuan awal matematis (KAM), tes kemampuan pemahaman matematis, tes kemampuan kreativitas matematis. Data dianalisis dengan uji ANAVA dua jalur. Hasil analisis data menunjukkan bahwa rata-rata tes kemampuan kreativitas eksperimen dan kontrol adalah 13,3 dan 7,58 dengan p-value (2-tailed) adalah 0, dengan 0 < α = 0,05 maka terdapat perbedaan kemampuan kreativitas matematik siswa yang diajarkan dengan Model Pencapaian Konsep (MPK) dan Pendekatan Pembelajaran Konvensional, nilai signifikan sebesar 0,732, karena 0,732 > 0,05 maka tidak ada interaksi antara pendekatan pembelajaran dengan kemampuan awal siswa terhadap perbedaan kemampuan kreativitas matematik siswa. .

PERBANDINGAN KEMAMPUAN KOMUNIKASI MATEMATIS SISWA YANG MENGGUNAKAN METODE SILIH TANYA BERBANTUAN KARTU MODEL DAN METODE MAKE A MATCH

2020 ◽  
Vol 2 (1) ◽  
pp. 32
Author(s):  
Ahmad Farham Majid ◽  
Ismail Ismail ◽  
Mardhiah Mardhiah ◽  
Fitriani Nur
Keyword(s):  
Total Sample ◽  
Control Group ◽  
Match Method ◽  
Mathematical Communication ◽  
Control Group Design ◽  
Purposive Sampling ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Quasi Experimental

AbstrakPenelitian ini bertujuan untuk mengetahui kemampuan komunikasi matematis siswa yang menggunakan metode silih tanya berbantuan kartu model dan metode make a match. Jenis penelitian ini adalah quasi eksperimen dengan desain penelitian non equivalent pretest-posttest control group design. Populasi dalam penelitian ini adalah seluruh siswa kelas VII SMPN 4 Sungguminasa Kab. Gowa berjumlah 351 siswa dan total sampel berjumlah 64 siswa dengan teknik pengambilan sampel purposive sampling. Instrumen yang digunakan dalam penelitian ini adalah tes yang terdiri dari pretest dan posttest dan non tes berupa lembar observasi. Berdasarkan hasil analisis data nilai siswa menggunakan statistik deskriptif rata-rata nilai kemampuan komunikasi matematis siswa yang diajar menggunakan metode silih tanya berbantuan kartu model adalah 86,84 dengan kategori sedang dan yang diajar menggunakan metode make a match adalah 77,78 dengan kategori sedang. Berdasarkan analisis statistik inferensial bahwa terdapat perbedaan yang signifikan antara kemampuan komunikasi matematis siswa yang menggunakan metode silih tanya berbantuan kartu model dengan yang menggunakan metode make a match pada kelas VII SMPN 4 Sungguminasa Kab. Gowa. AbstractThis study aims to determine the mathematical communication ability of students who use card-assisted questions method and make a match method. This type of research is a quasi-experimental research with nonequivalent pretest-posttest control group design. The population in this study were all VII grade students of SMPN 4 Sungguminasa, Gowa Regency with 351 students and a total sample of 64 students with purposive sampling techniques. The instrument used in this study was a test consisting of pre-test and post-test and non-test that is observation sheet. Based on the data analysis results that average the value of students’ mathematical communication skills who were taught using the card-assisted questions was 86.84 in the medium category and those who taught using make a match method were 77.78 in the medium category. The results of inferential statistical analysis that there is a significant difference between the mathematical communication abilities of students who use card-assisted questions and using the make a match method in class VII of SMPN 4 Sungguminasa, Gowa Regency.

The effect of patience training on university students’ patience and life satisfaction levels

2021 ◽  
Vol 9 (3) ◽  
pp. 712-719
Author(s):  
Ayşe Eliüşük Bülbül ◽  
Keyword(s):  
Life Satisfaction ◽  
University Students ◽  
Wilcoxon Test ◽  
Control Group ◽  
Control Group Design ◽  
Satisfaction Scale ◽  
Significant Difference ◽  
Post Test ◽  
And Control ◽  
Experimental Group

The purpose of this study is to determine the effect of the "patience training program" on the patience and life satisfaction levels of university students. The study was organized as a pre-test, post-test experiment and control group design. For a total of 30 students from which were 17 in the control group and 17 in the experimental group, patience training was given for 5 weeks. The "Patient Scale" developed by Schnitker (2010) and adapted to Turkish by Eliüşük and Arslan (2016) and the "Life Satisfaction Scale" developed by Diener, Emmons, Larsen and Griffin (1985) and adapted to Turkish by Dağlı and Baysal (2016) were used as a data collection tool in the study. The "Wilcoxon" test was used for the comparison of in-group differences in the analysis of obtained data and the "Mann-Whitney U" test was used in examining the differences between the two groups. As a result of the study, it was observed that the patience and life satisfaction average scores of students in the experimental group receiving "patience training" increased significantly, while there was no significant difference in the patience and life satisfaction averages scores of the control group students.

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