scholarly journals POTENTIAL EXTRACT of PARE FRUIT (Momordica charantia) AGAINST DIFFERENTIAL LEUKOCYTE IN CARP (Cyprinus carpio) WHICH INFECTED WITH Aeromonas hydrophila

2021 ◽  
Vol 1 (1) ◽  
pp. 1-5
Author(s):  
Sri Andayani ◽  
Ellana Sanoesi ◽  
Oktaviani Setya

Carp (C. carpio) in intensive production causes fish to be susceptible to disease, one of which is caused by A. hydrophila bacteria. Prevention is often done by giving synthetic chemical antibiotics that can be left in fish meat. Another alternative is to offer natural ingredients, one of which is bitter melon. The aim of the study was to determine the effect of bitter melon extract (M.charantia) on differential leukocytes (lymphocytes, monocytes and neutrophils) in carp (C. carpio) infected with A. hydrophila bacteria. This research method used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used were the administration of different extract doses, namely: treatment A (550 ppm), treatment B (650 ppm), treatment C (750 ppm), and treatment D (850 ppm). positive (K+) with bacterial infection of 107 cells/ml and administration of antibiotics chloramphenicol 0.03 mg/L and negative control (K-) with bacterial infection of 107 cells/ml. Blood collection when the fish are healthy, after offering the bitter gourd extract and after being infected. The results showed that bitter melon extract had a significantly different effect on leukocyte differential. The highest value obtained from treatment D (850 ppm) with the percentage of lymphocytes (18.67-28)%. neutrophils (2.32 = 6.0)% and monocytes (21-26.67)%. In conclusion, treatment with D dose of 850 ppm used as an immunostimulant can increase the differential leukocytes.

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Eka Kusmawati ◽  
Hari Santoso ◽  
Ahmad Syauqi

Meat elasticity is a characteristic of meat that has the power to resist pressure from the outside and return to its original shape. Tilapia fish (Oreochromis mossambicus) has perishable properties if it hasn’t been through processing. One alternative to maintain the suppleness of the meat by using a soaking mangosteen (Garcinia mangostana L.) peel. Based on the description above this study aims to knowing the effectiveness of soaking using mangosteen peel and analyzes the concentration for the best meat suppleness. The research method used was completely randomized design using 4 concentration of treatments 0%, 5%, 10%, 15% and  each with 5 replications. The results of study obtained Fcou = 72,55 ; F(0,01) = 5,29 shows that Fcou > F(0,01) so H0 is rejected, which means there is a very real difference between treatments with value concentration of 0% 5,069 N/mm2; concentration of 5% 7,574 N/mm2; concentration of 10% 10,401 N/mm2 and  concentration of 15% 13,924 N/mm2. The secondary metabolites in the mangosteen peel are effective so as to give effect to increase the tenderness of tilapia fish and the test results of the effective concentration value for the suppleness of the best fish meat according to SNI is the concentration of 15%. In this elascity test it was found thet the higher of concentration of mangosteen peel given the higher the level of elasticity of tilapia fish meat.Key Word: Elasticity, Immersion, Powder ConcentrationABSTRAKKekenyalan daging merupakan sifat daging yang mempunyai kekuatan untuk menahan tekanan dari luar dan kembali ke bentuk semula. Daging ikan mujair (Oreochromis mossambicus) memiliki sifat mudah rusak jika belum melalui suatu pengolahan. Salah satu alternatif untuk menjaga kualitas kekenyalan dagingnya dengan menggunakan rendaman kulit buah manggis (Garcinia mangostana L.). Tujuan penelitian ini adalah untuk mengetahui efektifitas perendaman menggunakan kulit buah manggis dan menganalisa konsentrasi yang tepat untuk kekenyalan daging ikan mujair. Metode penelitian yang digunakan yaitu Rancangan Acak Lengkap menggunakan 4 konsentrasi perlakuan 0%, 5%, 10%, 15% dan masing-masing 5 kali ulangan. Hasil penelitian didapat Fhit = 72,55 ; F(0,01) = 5,29 menunjukkan bahwa Fhit > F(0,01) sehingga H0 ditolak yang berarti terdapat beda sangat nyata antar perlakuan, dengan nilai konsentrasi 0% 5,069 N/mm2; konsentrasi 5% 7,574 N/mm2; konsentrasi 10% 10,401 N/mm2 dan konsentrasi 15% 13,924 N/mm2. Senyawa metabolit sekunder pada kulit manggis yang efektif sehingga memberikan pengaruh untuk meningkatkan kekenyalan daging ikan mujair dan hasil uji nilai konsentrasi yang efektif untuk kekenyalan daging ikan terbaik sesuai dengan SNI adalah konsentrasi 15%. Pada uji kekenyalan ini didapatkan bahwa semakin tinggi konsentrasi kulit manggis yang diberikan maka semakin tinggi tingkat kekenyalan daging ikan mujair.Kata Kunci: Kekenyalan, Perendaman, Konsentrasi Serbuk


2020 ◽  
Vol 20 (3) ◽  
pp. 1038
Author(s):  
Maryani Maryani ◽  
Kartika Bungas ◽  
Hasan Anwar

Saluang Belum Root (Lavanga sarmentosa) and Yellow Root ((Arcangelisia flava) Merr) plants that have antibacterial effects need to be proven, for this research this program uses Saluang Belum Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava) Merr) against bacterial inhibition. Aeromonas hydrophila by In Vitro. The method used in this study was a Completely Randomized Design (CRD) with 6 (six) settings and 3 (three) replications related to the aid of A = 25 gr / 150 ml distilled water, treatment B = 35 gr / 150 ml distilled water, C treatment = 45 gr / 150 ml distilled water, treatment D = 55 gr / 150 ml distilled water, handling E = 500 mg chloromphenicol / 150 ml distilled water, and treatment F = negative control only using the use of 150 ml distilled water. The results of this study indicate a combination of Saluang Belum Root Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava Merr) which is able to inhibit the growth of My Aeromonas hydrophila bacteria because it has an antibacterial effect, this can be seen from the results of the zone average (inhibitory power) on each The treatment is the highest clear zone according to the D 55 gr / 150 ml treatment with an average clear zone of 15 mm and the lowest clear zone yield is A 25 gr / 150 ml approval with an average clear zone value of 11 mm.  Keywords: Aeromonas hydrophila; Root of Saluang Not yet; Yellow Root; Antibacterial.


2018 ◽  
Vol 6 (2) ◽  
pp. 177-192
Author(s):  
Mery Valeta ◽  
Ade Dwi Sasanti ◽  
Yulisman .

ABSTRACTOne of problem on snakehead culture is lower value of survival and growth because of disease occurence. The aim of this research was to know the effect of different feeding time duration on survival and growth rate of snakehead fed by black cumin challenged by A.hydrophila. This research conducted from August until September 2015 in Laboratorium Budidaya Perairan, Aquaculture Study Program, Faculty of Agriculture, Sriwijaya University. The research method used Completely Randomized Design (CRD) with seven treatments and three replications : P1(fed without black cumin during 30 days no challenged), P2 (fed without black cumin during 30 days challenged by A. hydrophila), P3 (fed contain black cumin during 30 days no challenged), P4 (fed contain black cumin during 30 days challenged by A. hydrophila), P5 (fed contain black cumin during 21 days challenged by A. hydrophila), P6 (fed contain black cumin during 14 days challenged by A.hydrophila), and P7 (fed contain black cumin during 30 days challenged by A. hydrophila). The result of the research showed that snakehead fed by black cumin during 14 days (P6) challenged by A. hydrophila showed the highest value of survival rate, and growth, each sequentially were 93.33%, 5.28 g, and 1.32 cm. Keywords : snakehead, black cumin, survival rate, growth


2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen


2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.


PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus


Author(s):  
Ayi Yustiati ◽  
Syakirah Imtinan Zurwana ◽  
Achmad Rizal ◽  
Yuli Andriani

The purpose of this research is to determine the optimal dose of the addition of red spinach powder to artificial feed on the brightness of clown loach. This research was conducted at Aquaculture Laboratory Building 4 Faculty of Fisheries and Marine Sciences of Universitas Padjadjaran from April to May 2020. This research method is experimental with a Completely Randomized Design consisting of four treatments and three replications. The red spinach powder addition treatment used 0%, 2%, 4%, and 6% of the feed amount. The parameters observed are color value as primary data by using Toca Color Finder, while the growth, survival rate, and water quality as support data. Color assessment results were analyzed using the Kruskal-Wallis test, if there were significant differences, Z test would be performed. Growth data and survival rates were analyzed using Analysis of Variance (ANOVA). Because the F test results were not significantly different, the Duncan test was not carried out. The results showed that the addition of  6% red spinach powder is the best treatment with an increased color brightness value of 5,63.


2018 ◽  
Vol 12 (1) ◽  
pp. 31-51
Author(s):  
Aprilianto Bayu Arisandy ◽  
Agustina Widyasworo ◽  
Eddy Trijana Sudani

ABSTRACT The research was conducted at Mrs. Sripurwanti's farm in Rejotangan Village, Rejotangan Sub-district, Tulung Agung Regency. The research was conducted from 1 March to 1 April 2017. The aim of the research to determine the difference in the solution of Vitamin B Complex to the results of domestic chicken include hatchability, mortality and hatching weight. Research material using selected 225 eggs of chicken eggs and Vitamin B Complex as many as 30 grains. The research method used is experiments with Completely Randomized Design (RAL). The study was divided into 5 treatments and 5 replications per repeat consisting of 9 eggs. Treatment consists of the addition of Vitamin B complex as much as 0, 45 mg, 90 mg, 135 mg, and 180 mg The results are expected can provide information about alternative moisture enhancer ingredients namely Vitamin B Komplek. The results showed that vitamin B complex was not significant (P> 0.05) to hatchability, live eggs and die 2nd candling , mortality, and hatching weight. Vitamin B complex has significant effect (P <0,05) on hatchability and mortality based on candling to 2. Result of the research can be concluded that giving Vitamin B Complex as much as 45 mg deserves to be additive addition of humidity because it can increase the percentage of hatchability by 15%


2020 ◽  
Vol 8 (3) ◽  
pp. 17
Author(s):  
Kristi Aji Sugiarto ◽  
Desy MH Mantiri ◽  
Nickson J Kawung ◽  
Rizald M Rompas ◽  
Sandra O Tilaar ◽  
...  

Heavy metal is a very dangerous pollutant waste for it cannot be destroyed (non-degradable) and over time it will accumulate in the ocean. This study aims to determine the lead content in sediment, water and algal thallus as well as to determine the algae growth that has been treated with lead. The research method used was a completely randomized design (CRD) which consisted of 3 treatments, using different treatments for each sample. The highest metal content obtained in the sediment was 5,124 ppm, in the water was 0.120 ppm, and in the algae thallus was 4,816 ppm respectively. The results showed that Halimeda opuntia algae can grow even in an environment contaminated with lead heavy metal and can become a bioremediation agent by absorbing lead heavy metal. Keywords: Halimeda opuntia, Lead Heavy Metal, Algae Culture, Bioremediation             Logam berat merupakan salah satu limbah pencemar yang sangat berbahaya karena tidak dapat dihancurkan (non degradable) dan dapat terakumulasi di perairan laut. Penelitian ini bertujuan untuk mengetahui kandungan timbal pada sedimen, air dan thallus alga yang dikultur serta mengetahui pertumbuhan alga yang telah diberi perlakuan timbal. Metode penelitian mengunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan, dengan menggunakan perlakuan yang berbeda pada tiap sampel dan kontrol. Kandungan logam tertinggi yang didapatkan pada sedimen yaitu sebesar 5,124 ppm, pada air sebesar 0,120 ppm, dan pada thallus alga sebesar 4,816 ppm. Hasil yang diperoleh yaitu alga Halimeda opuntia dapat bertumbuh walaupun dalam lingkungan yang tercemar logam berat timbal serta mampu menjadi agen bioremediasi dengan menyerap logam berat timbal. Kata kunci: Halimeda opuntia, Logam BeratTimbal, Kultur Alga, Bioremediasi


2021 ◽  
Vol 888 (1) ◽  
pp. 012054
Author(s):  
A Jaelani ◽  
N Abelina ◽  
R Samudera ◽  
T Rostini ◽  
Masganti ◽  
...  

Abstract This research aims to study and analyze the effect of adding turmeric flour (Curcuma domestica Val) in the rations on the performance of 70 weeks old Isa Brown strain of laying hens. The research method used in this study was an experimental method with a completely randomized design (CRD) step with five treatments and four replications. The number of chickens used was as many as 40 laying hens. The treatments given to the sample were P0 = feed without giving turmeric flour (control), P1 = feed with 0.3% turmeric flour, P2 = feed with 0.5% turmeric flour, P3 = feed with 0.7% turmeric flour and treatment P4 = feed by giving turmeric flour 0,9%. The results showed that giving turmeric flour in the rations of the Isa Brown strain of laying hens affected chicken performance and egg quality. This research concludes that giving turmeric flour a percentage of 0.7% in Isa Brown’s 70-week old laying hens is the best for ration consumption, Hen Day Production, egg weight, feed conversion, and egg quality: Haught Unit, yolk egg color, yolk egg index, grading egg and egg mass. Based on the haugh unit value, all eggs produced are still in the AA grade category


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