scholarly journals Evaluasi Suplementasi Indigofera zollingeriana Sebagai Sumber Green Protein concentrate Terhadap Produksi Gas Metan, Amonia dan Sintesis Protein Mikroba Rumen

2021 ◽  
Vol 21 (3) ◽  
pp. 1455
Author(s):  
Afzalani Afzalani ◽  
R.A Muthalib ◽  
Rahmi Dianita ◽  
Fachroerrozi Hoesni ◽  
Raguati Raguati ◽  
...  
Keyword(s):  
Protein Production ◽  
Basal Diet ◽  
Gas Production ◽  
Metabolizable Energy ◽  
High Protein ◽  
Protein Source ◽  
Protein Concentrate ◽  
Microbial Protein ◽  
Indigofera Zollingeriana

The use of protein with low-cost, high quality, low methane, and ammonia emissions are a prerequisite as a protein source in ruminant. However, the European Commission has prohibited protein derived from fish meals for ruminant feeds. So encouraging efforts to explore the other protein sources to be most important. Most of the high protein legumes grow in tropical areas such as Indonesia and have the potential as an alternative protein source in ruminant feed, including Indigofera zollingeriana (25-27% protein content). But many browse legumes with high protein are a heterogeneous group of plants, with variable secondary metabolic content and rumen degradable protein. The aim of this experiment was to evaluate the characteristics fermentation of IZ as green protein supplement on in vitro methane, ammonia and microbial protein production. The experiment was a completely randomized design with four different level supplementation of Indigofera zollengeriana (IZ) as green protein concentrate and five replications. The treatment diets were R0; basal diet (60% forage + 40% concentrate) + 0% IZ, R1; R0 + 10% IZ, R2; R0 + 20% IZ, and R3; R0 + 30% IZ. The experiment result showed that supplemenatation of IZ was significant effects (P<0.05) to increase total gas, ammonia (N-NH3), total volatile fatty acid (TVFA), and metabolizable energy (ME) and significant effect (P<0.05) to decrease of methane and methane percentage. Supplementation IZ at a level of 10% was significantly higher for dry matter digestibility (DMD), organic matter digestibility (OMD), and microbial protein production (PPM) than diets treatment of R0, R2, and R3. The experiment concluded that Supplementation of I. zollingeriana (IZ) was able to reduce the methane gas production. Protein characteristics of IZ have easily degradable by rumen microbe showed the ammonia production was linearly increasing by 45.66% for each increasing level of IZ supplementation. Microbial protein production was higher (184.33 mg/ml) obtained of IZ supplementation up to 10% (R1). The experiment suggests doing protected protein of IZ when be used as a protein source in ruminant diets.

scholarly journals Effect of Commercial Slow-Release Urea Product on in Vitro Rumen Fermentation and Ruminal Microbial Community Using RUSITEC Technique

2021 ◽  
Author(s):  
Yongmei Guo ◽  
Ling Xiao ◽  
Long Jin ◽  
Sumei Yan ◽  
Dongyan Niu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Dairy Cow ◽  
Protein Production ◽  
Control Diet ◽  
Gas Production ◽  
Microbial Protein ◽  
N Concentration ◽  
Slow Release Urea ◽  
Microbial N

Abstract Background The objectives of this study were to determine the effect of commercial slow-release urea (SRU) on in vitro fermentation characteristics, nutrient digestibility, gas production, microbial protein synthesis and bacterial community using rumen simulation technique (RUSITEC). The experiment was a completely randomized design with four treatments and four replications of each treatment. Treatments were: control diet (no SRU addition), control diet plus 0.28% SRU (U28), or plus 0.56% SRU (U56), and control diet that was modified for substituting with 0.35% SRU for equavelant soybean protein (MU35; dry matter [DM] basis). The experiment consisted of 8 days of adaptation and 7 days of data and sample collection. Rumen inoculum was obtained from three ruminally fistulated Angus cows fed the same diet to the substrate incubated. Results Digestibility of DM, organic matter (OM), crude protein (CP), fibre and starch was not affected, but daily production of gas (P < 0.07) and methane (P < 0.05) was quadratically changed with increasing SRU supplementation. The increase of SRU addition did not affect fermentation pH and total volatile fatty acid (VFA) production, whereas linearly (P < 0.01) decreased proportion of propionate, and linearly (P < 0.01) increased acetate to propionate ratio and ammonia nitrogen (N) concentration. The microbial N efficiency also linearly (P < 0.03) improved with increasing supplementation of SRU. In comparison with control diet, the dietary substitution of SRU for part of soybean meal increased (P < 0.05) the digestibility of DM, OM and CP and decreased (P < 0.02) the total gas production. The total VFA production and acetate to propionate ratio did not differ between control and MU35, whereas the proportion of butyrate was lower (P < 0.05) and that of branched-chain VFA was greater (P < 0.05) with MU35 than control diet. Total and liquid-associated microbial N production as well as ammonia N concentration were greater (P < 0.03) with MU35 than control diet. Observed OTUs, Shannon diversity index, and beta diversity of the microbial community did not differ among treatments. Taxonomic analysis revealed no effect of adding SRU on the relative abundance of bacteria at the phylum level, while at the genus level, the impact of SRU addition on microbial community was greater with MU35 either for liquid associate bacteria or feed particle-associated bacteria. Conclusions Supplementation of a dairy cow diet with SRU showed potential of increase in ammonia N concentration and microbial protein production, and change fermentation pattern to more acetate production. Adding SRU in dairy cow diet also showed beneficial effect on improving digestibility of OM and fibre. The results suggest that SRU can partially substitute soybean meal in dairy cow diet to increase microbial protein production without impairing rumen fermentation.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

scholarly journals Produk Fermentasi Rumen dan Produksi Protein Mikroba Sapi Lokal yang Diberi Pakan Jerami Amoniasi dan Beberapa Bahan Pakan Sumber Energi

2011 ◽  
Vol 11 (2) ◽  
pp. 29-34 ◽  
Author(s):  
Novita Hindratiningrum ◽  
Muhamad Bata ◽  
Setya Agus Santosa
Keyword(s):  
Protein Synthesis ◽  
Rice Bran ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Metabolic Energy ◽  
Energy Sources ◽  
Microbial Protein ◽  
Microbial Protein Synthesis

Products of rumen fermentation and protein microbial of dairy cattle feed with rice bran ammonization and some feedstuffs as an energy sourcesABSTRACT. This study aims to examine the energy sources of feed ingredients that can increase the production of Volatile Fatty Acids (VFA), N-NH3, microbial protein synthesis, total gas production and metabolic energy. The material used is as a source of rumen fluid inoculum from Frisian Holstein cows (FH) females, amoniasi rice straw, salt, mineral mix brand "Ultra Minerals' production Eka Farma Semarang, onggok wet and dry, corn, and rice bran. Observed variable is the concentration of (VFA), N-NH3, rumen microbial protein synthesis, and total gas production. Based on the analysis of diversity seen any significant effect (P0.05) on total VFA concentration, N-NH3 and total gas but had no effect (P0.05) on microbial protein synthesis. Conclusion of research is the provision of energy sources with rice bran treatment, onggok wet and dry corn flour can be used as fermentable carbohydrates on feed hay amoniasi in vitro.

PSI-9 Digestibility and metabolizable energy content of supplemented diets based on native shrubs and buffelgrass for small ruminants in northeastern Mexico

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 275-276
Author(s):  
Víctor Manuel Perrusquía Tejeida ◽  
Nydia Corina Vásquez Aguilar ◽  
Humberto González Rodríguez ◽  
Hugo Bernal Barragán ◽  
Fernando Sánchez Dávila ◽  
...  
Keyword(s):  
Energy Content ◽  
Artificial Saliva ◽  
Small Ruminants ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Acacia Farnesiana ◽  
Dry Matter Digestibility ◽  
Northeastern Mexico ◽  
Native Shrubs

Abstract The objective of this study was to evaluate the effect of supplementing 10% of sorghum grain (SG), soybean meal (SBM) or dried citrus pulp (DCP) upon dry matter digestibility (DMD), organic matter digestibility (OMD), and metabolizable energy content (ME) of diets based on dried leaves of two shrub species (Acacia farnesiana and Acacia rigidula) and one grass (Cenchrus ciliaris) browsed by small ruminants. Twelve experimental diets were elaborated using three forage sources: C. ciliaris, A. farnesiana and A. rigidula and evaluated either alone (0% supplementation) or supplemented with 10% of SG (9.0% CP, 22.3% NDF), SBM (42.8% CP, 16.3% NDF) or DCP (4.9% CP, 19.1% NDF). In vitro DMD (Daisy incubator, ANKOM) at 48 h, and in vitro gas production (GP, in calibrated 100 mL glass syringes) at 24 h, were determined by incubating samples in an inoculum prepared by using rumen liquor of two rumen fistulated sheep and artificial saliva in a 1:4 ratio. OMD and ME content were calculated from the gas production, crude protein, ash, and ether extract content. Data were evaluated according to a 3 x 4 factorial experiment. There were no significant (P &gt; 0.05) forage source x supplement interaction effects. Leaves of A. farnesiana had higher (P &lt; 0.05) DMD (64%), GP (26 mL), ME (1910 kcal ME/kg DM), and OMD (53%) than other forages (mean 47% DMD, 17 mL GP, 1232 kcal ME/kg DM, and 39% DMO). Supplements increased (P &lt; 0.05) DMD (average +8%), but only DCP increased (P &lt; 0.05) the GP (+23%) of evaluated forages. Leaves of A. farnesiana had higher DMD (P &lt; 0.05; 64%). There were no effects (P &gt;0.05) of the 10% added supplements in ME content or DMO. In conclusion, leaves of A. farnesiana and DCP are valuable sources of nutrients and energy for small ruminants in northeastern Mexico.

scholarly journals Chemical-bromatological composition and in vitro ruminal kinetics of sugar cane silage with maniçoba

2018 ◽  
Vol 40 (1) ◽  
pp. 42569
Author(s):  
Francisco Allan Leandro de Carvalho ◽  
Percivaldo Xavier Resende ◽  
Clístenes Amorim Benicio ◽  
Jackson De Oliveira Siqueira ◽  
Daniel Ribeiro Menezes ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Nutritive Value ◽  
Degradation Kinetics ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Inclusion Level ◽  
Kinetics Of

The objective this study was to evaluate the effect of maniçoba supplementation in sugar cane silage with respect to chemical-bromatological composition and the in vitro degradation kinetics of the silage. This experiment was conducted in a completely randomized design with four treatments (maniçoba levels: 0, 20, 30, and 40%) and six repetitions. Silage samples were analyzed for their chemical-bromatological composition, digestible energy, metabolizable energy, total digestible nutrients, in vitro gas production and degradability parameters. The silage with higher inclusion level had better bromatological composition (p < 0.05) than the silage without maniçoba for CP, NDF, ADF and MM (6.49, 56.64, 38.66 and 4.52% versus 2.21, 70.96, 49.95 and 2.78%). Higher ME content (2.35 MJ kg-1 MS versus 1.85 MJ kg-1 MS), DE (2.87 Mcal kg-1 MS versus 2.25 Mcal kg-1 MS) and TDN (65.16% versus 51.11%), respectively. The highest values for gas production were also observed in silage with added maniçoba due to higher NFC content (34.87%). With an increase in the proportion of maniçoba, there was an increase in the soluble a fraction, b fraction, and thus a higher effective degradability of dry matter (46.56%). The addition of maniçoba improves the nutritive value of sugarcane silage.  

Influence of ionophores on in vitro fermentation by rumen fluid from sheep receiving yeast culture (Yeasacc; YC)

1991 ◽  
Vol 1991 ◽  
pp. 78-78
Author(s):  
C.J. Newbold ◽  
R.J. Wallace ◽  
I.M. Nevison
Keyword(s):  
Feed Efficiency ◽  
Rumen Fluid ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
Animal Production ◽  
Yeast Culture ◽  
Microbial Protein ◽  
In Vitro Fermentation ◽  
Wide Range

A wide range of compounds has been described which have the potential to improve animal production by manipulating the rumen fermentation. Prominent among these rumen modifiers are the ionophores. Ionophores, such as monensin and tetronasin, improve feed efficiency, partly by increasing the flow of amino-N from the rumen and partly by stimulating the production of propionate in the rumen with an associated reduction in the production of methane (Russell and Strobel, 1988). Recently there has been increasing interest in the use of yeast culture (YC) and other fungal preparation to modify the rumen fermentation. These products have been shown to increase bacterial numbers within the rumen with an associated increase in the breakdown of fibre and supply of microbial protein (Williams and Newbold, 1990). YC has also been reported to increase the production of propionate in the rumen. Little appears to be known about the effect a combination YC and an ionophore would have on the rumen fermentation. This study describes the effects of the ionophores monensin and tetronasin on the fermentation of hay by rumen fluid from sheep fed a basal diet with or without YC.

Effect of Stylosanthes guianensis supplementation on intake and nitrogen metabolism of Bos indicus cattle offered a basal diet of mixed rice straw and tropical grass

2013 ◽  
Vol 53 (5) ◽  
pp. 453 ◽  
Author(s):  
Miranda Pen ◽  
Darryl B. Savage ◽  
John V. Nolan ◽  
Mom Seng
Keyword(s):  
Rice Straw ◽  
Protein Production ◽  
Oryza Sativa L ◽  
Bos Indicus ◽  
Basal Diet ◽  
Ammonia Concentration ◽  
Microbial Protein ◽  
Total N ◽  
Stylosanthes Guianensis ◽  
Tropical Grass

The effect of supplementing a mixed rice straw and tropical grass diet with legume as a nitrogen (N) source on intake, digestibility, rumen ammonia and microbial protein production was evaluated in Bos indicus cattle. Four rumen-cannulated steers were used in a crossover design with two diets and two periods. The diets were T1 = 40% rice (Oryza sativa L.) straw + 60% grass (Brachiaria spp. cv. Mulato II hybrid) and T2 = 40% rice straw + 30% grass + 30% legume (Stylosanthes guianensis cv. CIAT 184) on DM basis. Supplementation with legume doubled (P < 0.01) rice straw and total N intake, and increased total DM intake by 32%. It did not affect the DM, organic matter, neutral detergent fibre and acid detergent fibre digestibility (P > 0.05) but did increase (P < 0.05) N digestibility. Faecal N and total N outputs from T2 cattle were higher (P < 0.05) than T1 cattle, but urinary N output did not differ between diets (P > 0.05). N retention in T2 cattle was improved by 83% (P < 0.05) compared with T1 cattle. Rumen ammonia concentration, microbial protein production and efficiency of microbial protein production were improved (P < 0.05) when the legume forage was included in the straw–grass diet. We conclude that when a mixed rice straw and fresh grass diet is supplemented with ~30% legume (DM basis), significant improvements in DM and N intake can be achieved.

scholarly journals The efficiency of microbial protein synthesis in the rumen and the degradability of feed nitrogen between the mouth and abomasum in steers given different diets

1984 ◽  
Vol 51 (1) ◽  
pp. 77-83 ◽  
Author(s):  
A. B. McAllan ◽  
R. H. Smith
Keyword(s):  
Protein Synthesis ◽  
Fish Meal ◽  
Basal Diet ◽  
Metabolizable Energy ◽  
Diaminopimelic Acid ◽  
Barley Straw ◽  
Microbial Protein ◽  
Total N ◽  
Microbial Protein Synthesis ◽  
Mixed Bacteria

1. Protozoa-free steers with simple rumen and abomasal cannulas were given basal diets consisting of a concentrate mixture of flaked maize and tapioca with barley straw (BS) or alkali-treated barley straw (BSA). Other diets were supplemented with urea (BSU and BSAU respectively) or with fish meal replacing the tapioca (BSF and BSAF respectively). The diets were isoenergetic and calculated to provide sufficient metabolizable energy (ME) to support a growth rate of 0.5 kg/d. Rumen-degradable nitrogen (RDN): ME values (g/MJ) were estimated to be 0.50, 1.20 and 0.80 for the basal diet, urea- and fish-meal-supplemented diets respectively. RNA and α, ε-diaminopimelic acid (DAP) were used as microbial markers. 103Ruthenium and polyethylene glycol (PEG) were given as flow markers and flows (g/24 h) at the abomasum of organic matter (OM) and nitrogenous constituents were calculated.2. Samples of mixed bacteria separated from rumen digesta from animals receiving N-supplemented diets contained significantly more N than those from animals receiving basal diets (approximately 74 and 62 mg/g dry matter (DM) respectively) but there were no other significant differences in total-N contents between treatments. RNA-N: total-N values were similar for all diets (approximately 0.13). DAP-N: total-N values were significantly lower in bacteria from animals receiving alkali-treated (AT) rather than untreated (UT) straw (approximately 0.008 and 0.011 respectively).3. The proportion of OM intake digested in the rumen (ADOM) was significantly higher for animals receiving AT straw rather than UT straw (approximately 0.54 and 0.43 respectively). N supplementation had no effect on OM digestibility.4. When basal rather than N-supplemented diets or AT-straw- rather than UT-straw-containing diets were given, there were significantly lower flows of ammonia-N, non-ammonia-N (NAN) and microbial-N (based on RNA flow, MN(RNA)) at the abomasum. Mean daily MN(RNA) flows (g) were 21, 30, 31, 16, 27 and 28 for diets BS, BSU, BSF, BSA, BSAU and BSAF respectively. These correspond to estimated efficiencies of microbial protein synthesis, expressed as g MN(RNA) /kg truly-digested OM, at 14, 22, 22, 12, 18 and 19 respectively. Values were significantly lower for basal as compared with corresponding N-supplemented diets and for AT-straw diets as compared with corresponding UT-straw diets.5. Estimated mean proportions of total feed-N intake degraded in the rumen, based on MN(RNA) as microbial marker, of diets BS, BSU and BSF were 0.60, 0.74 and 0.47 respectively; corresponding values for diets BSA, BSAU and BSAF were 0.72, 0.73 and 0.36 respectively. Making certain assumptions, the mean proportions of fish-meal-N digested in the rumen were calculated to be 0.23 and 0.14 respectively for UT- and AT-straw diets. The values were not significantly different. Values for microbial flows based on DAP as marker were significantly lower, by about 25%, than those based on RNA.

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