scholarly journals Quinolone Resistant Enterobacteriaceae and Pseudomonas in Environ-mental Water in Dhaka, Bangladesh

2017 ◽  
Vol 42 (1) ◽  
pp. 28-32
Author(s):  
K M Anwarul Haque ◽  
Kazuhiro Tateda ◽  
Yoshikazu Ishii ◽  
Qumrul Huda ◽  
Ruhul Amin Miah
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Surface Water ◽  
Potential Role ◽  
Environmental Influence ◽  
Dhaka City ◽  
Bacterial Strains ◽  
Resistant Genes ◽  
Surface Water Samples

Pathogens that carry antibacterial resistant genes represent a threat for failure of antibiotic therapy and are associated with high mortality, morbidity and expenses. In Bangladesh, although quinolone resistance in clinical infections has been reported, environmental influence to this resistance is poorly known. Thus, to examine the existence of quinolone resistant bacterial strains in surface water in Dhaka, the study was conducted during June 2012 to January 2014. Surface water samples from Dhaka city were screened and isolated quinolone resistant Pseudomonas putida, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Molecular and genetic analysis identified the resistant determinants in these isolates. The study suggests that potential role of water for the dissemination and transmission of resistant genes among microorganisms causing human and animal infections in Bangladesh.

scholarly journals MexAB-OprM Efflux Pump Interaction with the Peptidoglycan of Escherichia coli and Pseudomonas aeruginosa

2021 ◽  
Vol 22 (10) ◽  
pp. 5328
Author(s):  
Miao Ma ◽  
Margaux Lustig ◽  
Michèle Salem ◽  
Dominique Mengin-Lecreulx ◽  
Gilles Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Cell Division ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Efflux Pump ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Active Efflux

One of the major families of membrane proteins found in prokaryote genome corresponds to the transporters. Among them, the resistance-nodulation-cell division (RND) transporters are highly studied, as being responsible for one of the most problematic mechanisms used by bacteria to resist to antibiotics, i.e., the active efflux of drugs. In Gram-negative bacteria, these proteins are inserted in the inner membrane and form a tripartite assembly with an outer membrane factor and a periplasmic linker in order to cross the two membranes to expulse molecules outside of the cell. A lot of information has been collected to understand the functional mechanism of these pumps, especially with AcrAB-TolC from Escherichia coli, but one missing piece from all the suggested models is the role of peptidoglycan in the assembly. Here, by pull-down experiments with purified peptidoglycans, we precise the MexAB-OprM interaction with the peptidoglycan from Escherichia coli and Pseudomonas aeruginosa, highlighting a role of the peptidoglycan in stabilizing the MexA-OprM complex and also differences between the two Gram-negative bacteria peptidoglycans.

scholarly journals Analysis of Ground and Surface Water Samples from some Area of Dhaka City for Polycyclic Aromatic Hydrocarbons (PAHs)

2015 ◽  
Vol 63 (1) ◽  
pp. 59-60 ◽  
Author(s):  
S Mandal ◽  
N Khuda ◽  
MR Mian ◽  
M Moniruzzaman ◽  
N Nahar ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Surface Water ◽  
Aromatic Hydrocarbons ◽  
Water Samples ◽  
Dhaka City ◽  
Polycyclic Aromatic ◽  
Surface Water Samples

Abstract not available DOI: http://dx.doi.org/10.3329/dujs.v63i1.21770 Dhaka Univ. J. Sci. 63(1): 59-60, 2015 (January)

scholarly journals FLORAL EXTRACT OF Tibouchina granulosa (DESR.) COGN. PHYTOCHEMISTRY PROSPECTION, AND ANTIBACTERIAL ACTIVITY

2021 ◽  
Vol 5 (1) ◽  
pp. 1-9
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Matheus Vinícius Abadia Ventura ◽  
Carlos Frederico de Souza Castro
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Floral Organ ◽  
Salt Formation ◽  
Bacterial Strains ◽  
Positive Results

Tibouchina granulosa is a species that blooms annually in several regions of Brazil. This species is still little explored in terms of phytocompounds in all organs of this plant, especially the floral organ. Flowers of T. granulosa were collected in the municipality of Rio Verde, Goiás, Brazil, in 2021. The hydroethanolic floral extract was prepared by maceration and qualitative phytochemical (colorimetric reactions and salt formation) and antibacterial analyzes performed and the results expressed in millimeters of inhibition at different concentrations in mg mL-1. Several phytochemical classes were observed with positive results, especially for alkaloids, phenolics, oxylates, saponins, carbohydrates and tannins. As for the bacterial assay, potential antibacterial activity was observed for all bacterial strains tested, except for Salmonella serovar Thyphymurium and serovar Enteritidis. Expressive inhibitions were observed for Enterococcus faecalis > Pseudomonas aeruginosa > Staphylococcus aureus and Escherichia coli at the highest concentrations between 100-50 mg mL-1. The floral extract of Tibouchina granulosa showed phytotherapeutic potential with the presence of several phytochemical groups and expressive antibacterial activity.

scholarly journals ANTIFUNGAL AND ANTIBACTERIAL ACTIVITY OF ALOE VERA PLANT EXTRACT

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
P Danish ◽  
Q Ali ◽  
MM Hafeez ◽  
A Malik
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Aspergillus Niger ◽  
Bacillus Megaterium ◽  
Aloe Vera ◽  
Water Soluble ◽  
Root Extract ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Fungal Strains

Aloe vera is a well-known medicinal plant used in many therapeutic purposes. Naturally it is composed of many useful compounds that have ability to use for treatment of many diseases. The active compounds reported in this plant are saponins, sugar, enzymes, vitamins, aloesin, aloeemodin, aloin, acemannan aloemannan, aloeride, methylchromones, flavonoids, naftoquinones, sterols, minerals, anthraquinones, amino acids, lignin and salicylic acid and other different compounds including fat-soluble and water-soluble vitamins, enzymes, minerals, simple/complex sugars, organic acid and phenolic compounds. In this study aloe vera is used for antibacterial and antifulgal activity against different strains of bacteria and pathogenic fungal strains. Ethanol extract of Aloe vera leaves and roots is applied on these bacterial and fungal strains in different concentrations (15, 20, 25, 30µl). Bacillus cereus, Bacillus subtitis, Bacillus megaterium, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and some other bacterial strains are used for this study. Escherichia coli and Agrobacterium tumefacins shows zone of inhibition around 18mm which consider as good result. Bacillus subtitis and Bacillus megaterium also shows good result around 16mm. Proteus mirabilis and Pseudomonas aeruginosa shows minimum zone of inhibition which is around 11mm. among all used fungal strains (fuserium oxysporum, Candida albicans, Aspergillus fumigatus, Aspergillus niger) fuserium oxysporum and Aspergillus niger shows excellent results around 19mm both against root extract and leaves extract.

scholarly journals Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Daniyar Sadyrbekov ◽  
Timur Saliev ◽  
Yuri Gatilov ◽  
Ivan Kulakov ◽  
Roza Seidakhmetova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Spatial Structure ◽  
Bacterial Strains ◽  
Gram Negative ◽  
X Ray ◽  
Cyclopropane Derivative

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.

scholarly journals Functional Analysis of the Acinetobacter baumannii XerC and XerD Site-Specific Recombinases: Potential Role in Dissemination of Resistance Genes

Antibiotics ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 405
Author(s):  
David L. Lin ◽  
German M. Traglia ◽  
Rachel Baker ◽  
David J. Sherratt ◽  
Maria Soledad Ramirez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Acinetobacter Baumannii ◽  
Potential Role ◽  
Covalent Attachment ◽  
Recombination Reaction ◽  
Site Specific ◽  
Site Specific Recombination ◽  
E Coli ◽  
Resistant Genes

Modules composed of a resistance gene flanked by Xer site-specific recombination sites, the vast majority of which were found in Acinetobacter baumannii, are thought to behave as elements that facilitate horizontal dissemination. The A. baumannii xerC and xerD genes were cloned, and the recombinant clones used to complement the cognate Escherichia coli mutants. The complemented strains supported the resolution of plasmid dimers, and, as is the case with E. coli and Klebsiella pneumoniae plasmids, the activity was enhanced when the cells were grown in a low osmolarity growth medium. Binding experiments showed that the partially purified A. baumannii XerC and XerD proteins (XerCAb and XerDAb) bound synthetic Xer site-specific recombination sites, some of them with a nucleotide sequence deduced from existing A. baumannii plasmids. Incubation with suicide substrates resulted in the covalent attachment of DNA to a recombinase, probably XerCAb, indicating that the first step in the recombination reaction took place. The results described show that XerCAb and XerDAb are functional proteins and support the hypothesis that they participate in horizontal dissemination of resistant genes among bacteria.

scholarly journals The effect of human activities on the pollution of water in southwest Giza area, Egypt

2017 ◽  
Vol 17 (5) ◽  
pp. 1368-1376 ◽  
Author(s):  
Elsayed M. Abu El Ella ◽  
Ahmed A. Elnazer ◽  
Salman A. Salman
Keyword(s):  
Heavy Metals ◽  
Surface Water ◽  
Water Resources ◽  
Major Ions ◽  
Drinking And Irrigation ◽  
Groundwater Samples ◽  
Industrial Complexes ◽  
And Migration ◽  
Surface Water Samples

Southwest Giza area is one of the most complicated regions in Egypt because of the combination of agricultural, industrial and urbanization activities with few studies about water resources contamination with heavy metals. In this study, ten surface water samples and eight groundwater samples were collected and analyzed for pollution with Fe, Mn, As, Cr, Cd, Pb and Cu. The samples were collected randomly according to the topographic locations and accessibility. The surface water is suitable for both drinking and irrigation use according to its salinity (total dissolved solids, TDS < 500 mg/l) and content of major ions. Unfortunately, some samples contain concentrations of As, Cd, Cu and Pb higher than the WHO drinking water guidelines. The groundwater samples have TDS ranging from 204 to 2,100 mg/l. Also, the groundwater contains higher concentrations of Fe, Mn and As than surface water. The highest concentrations of heavy metals As, Cd and Pb were recorded in the desert fringes and close to the industrial complexes indicating the role of geological sediments in the transportation and migration of pollutants. The unconfined part of the Quaternary aquifer in the desert fringes is more vulnerable to contamination. The results of this study reflect the role of human and industrial activates in polluting water resources with heavy metals, which puts the aquatic environment in the study area under stress.

scholarly journals FpvA-Mediated Ferric Pyoverdine Uptake in Pseudomonas aeruginosa: Identification of Aromatic Residues in FpvA Implicated in Ferric Pyoverdine Binding and Transport

2005 ◽  
Vol 187 (24) ◽  
pp. 8511-8515 ◽  
Author(s):  
Jiang-Sheng Shen ◽  
Valérie Geoffroy ◽  
Shadi Neshat ◽  
Zongchao Jia ◽  
Allison Meldrum ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Three Dimensional ◽  
Binding Pocket ◽  
Dimensional Structure ◽  
Aromatic Residues ◽  
Three Dimensional Structure

ABSTRACT A number of aromatic residues were seen to cluster in the upper portion of the three-dimensional structure of the FpvA ferric pyoverdine receptor of Pseudomonas aeruginosa, reminiscent of the aromatic binding pocket for ferrichrome in the FhuA receptor of Escherichia coli. Alanine substitutions in three of these, W362, W391, and F795, markedly compromised ferric pyoverdine binding and transport, consistent with a role of FpvA in ferric pyoverdine recognition.

scholarly journals Patterns of Extended-Spectrum β-Lactamase Producing Uropathogens Detection in Tertiary Care Hospital of Bangladesh

2021 ◽  
pp. 29-34
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Human Life ◽  
Tertiary Care ◽  
Public Health Problem ◽  
Care Hospital ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Extended Spectrum ◽  
Resistance Patterns

It is a great concern that extended-spectrum β-lactamase (ESBL) and non-ESBL uropathogenic micro-organisms have been worldwide illustrated to increase multidrug resistance. To study the prevalence and patterns of uropathogens, and antimicrobial susceptibilities profiles of ESBL and non-ESBL producing bacterial infection in a tertiary level health service center of Bangladesh. The prevalence of ESBL producing uropathogens and their antimicrobial resistance patterns were identified in 176 isolates from patients with UTI. ESBL and non-ESBL producing bacterial isolates and their antibiotic sensitivity and resistance patterns were distinguished from the 176 patients of suspected urinary tract infection. The Double-disc diffusion test was done to determine the presence of ESBL-producing bacterial strains. The most widely ESBL positive uropathogen was Escherichia coli (87%), followed by Pseudomonas aeruginosa, (6.8%), Enterococcus spp. (3.4%), Acinetobacter spp. (2.5%) and non-ESBL positive E. coli (41.4), Staphylococcus saprophyticus (25.9%), Pseudomonas aeruginosa (17.2%), Staphylococcus aureus (10.3%), and Klebsiella pneumoniae (5.2%). The current investigation found most frequently Escherichia coli in both ESBL and non-ESBL uropathogenic groups as 87% and 41.4% respectively. Generally, a large number of antibiotic resistance patterns and ESBL producing common bacterial isolates were found in this study which increases the public health problem. Therefore, for safe human life, we ought to be taking appropriate action against the threat.

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