Pengaruh Pemberian Ekstrak Kijing (Pilsbryoconcha exilis) Pada Pemulihan Kualitas Sperma Mencit  (Mus musculus)

ABSTRACT[Effect of Kijing Extract (Pilsbryoconcha exilis) on the Recovery of Sperm Quality in Mice (Mus musculus)]. This study aims to determine the effect of Pilsbryoconcha exilis extract on the quality of M. muscullus sperm. The approach used for the study was experiment with a complete randomized design model. In this study, 4 treatments and 5 replications were conducted, including treatment 1 which only administered aquades as control (P0), treatment 2 which only given paracetamol dose 250 mg / kg (P1), and treatment 3 given paracetamol dose 250 mg / kg and P. exilis extract dose 0,46 mg / g (P2) and treatment 4 given paracetamol 250 mg / kg and extract P. exilis 0,69 mg / g (P3). Technique of data analysis using qualitative descriptive and statistical test of One Way Annova parametry, and if obtained significant difference then continued statistic test of Smallest Real Difference. From the results of the study, it was found that P. exilis and paracetamol extract had no effect on sperm quality. Keywords: Sperm; M. muscullus; extract P. exilis; paracetamol.(Received August 14 , 2018; Accepted January 20, 2019; Published February 26, 2019) ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak Pilsbryoconcha exilis terhadap kualitas sperma M. muscullus yang diberi parasetamol. Pendekatan yang digunakan untuk penelitian adalah eksperimen dengan model rancangan acak lengkap (RAL). Pada penelitian ini menggunakan 4 perlakuan dan 5 ulangan meliputi perlakuan 1 yang hanya diberikan aquades merupakan kontrol (P0), perlakuan 2 yang hanya diberikan parasetamol dosis 250 mg/kg bb (P1), dan perlakuan 3 yang diberikan parasetamol dosis 250 mg/kg bb dan ekstrak P. exilis dosisi 0,46 mg/g bb (P2) dan perlakuan 4 yang diberikan parasetamol 250 mg/kg bb dan ekstrak P. exilis 0,69 mg/g bb (P3). Teknik analisa data menggunakan deskriptif kualitatif dan uji statistik parametri One Way Annova, dan jika didapatkan perbedaan yang bermakna maka dilanjutkan uji statistic Beda Nyata Terkecil (BNT). Dari hasil penelitian diketahui pemberian ekstrak P. exilis dan parasetamol berepengaruh tapi tidak signifikan terhadap kualitas sperma. Kata Kunci: Sperma; M. muscullus; ekstrak P. exilis; parasetamol.

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Pengaruh Pemberian Ekstrak Kijing (Pilsbryoconcha exilis) Pada Pemulihan Kualitas Sperma Mencit (Mus musculus)

PENDIPA Journal of Science Education ◽

10.33369/pendipa.3.1.40-44 ◽

2019 ◽

Vol 3 (1) ◽

pp. 40-44

Author(s):

Erna Yunita Sari ◽

Bhakti Karyadi ◽

Aceng Ruyani ◽

Choirul Muslim

Keyword(s):

Data Analysis ◽

Mus Musculus ◽

Sperm Quality ◽

Statistical Test ◽

Design Model ◽

Real Difference ◽

Randomized Design ◽

Significant Difference ◽

Qualitative Descriptive

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PENGARUH PAPARAN ASAP ROKOK KRETEK TERHADAP KUALITAS SPERMATOZOA MENCIT JANTAN (MUS MUSCULUS)

Jurnal e-Biomedik ◽

10.35790/ebm.1.1.2013.4367 ◽

2013 ◽

Vol 1 (1) ◽

Author(s):

Immanuel Van Donn Batubara ◽

Benny Wantouw ◽

Lydia Tendean

Keyword(s):

Cigarette Smoke ◽

Mus Musculus ◽

Sperm Quality ◽

Cigarette Smoke Exposure ◽

Control Group ◽

Not Given ◽

Significant Difference ◽

Key Word ◽

Randomized Experimental Design

Abstrak: Asap rokok mengandung tiga komponen toksik utama, yaitu karbonmonoksida, nikotin, dan tar yang dapat menyebabkan gangguan pada spermatogenesis. Tujuan penelitian ini adalah untuk melihat pengaruh paparan asap rokok kretek terhadap kualitas spermatozoa mencit. Penelitian ini adalah penelitian eksperimental dengan rancangan acak lengkap. Subyek penelitian sebanyak 25 ekor mencit jantan (Mus musculus) yang dibagi secara acak menjadi 5 kelompok: kelompok P0 tidak diberikan paparan asap rokok, kelompok P1 diberikan paparan asap rokok kretek 1 batang, kelompok P2 diberikan paparan asap rokok kretek 2 batang, kelompok P3 diberikan paparan asap rokok kretek 3 batang, kelompok P4 diberikan paparan asap rokok kretek 4 batang. Perlakuan diberikan selama 30 hari. Hasil penelitian menunjukkan adanya perbedaan yang signifikan pada konsentrasi dan motilitas spermatozoa kelompok yang mendapatkan paparan asap rokok kretek (p<0,05) dibandingkan dengan kelompok kontrol. Morfologi spermatozoa kelompok P2,P3,P4 didapati hasil yang berbeda signifikan dengan kelompok kontrol (p<0,05) tetapi kelompok P1 hasilnya berbeda tak signifikan dengan kelompok kontrol. Paparan asap rokok kretek memberikan pengaruh negatif terhadap kualitas spermatozoa yang disebabkan oleh adanya radikal bebas yang dihasilkan oleh asap rokok. Kata Kunci: asap rokok, kualitas spermatozoa. Abstract: Cigarette smoke contains three main toxic components, namely carbon monoxide, nicotine, and tar that can cause disturbances in spermatogenesis. The objective of this study was to investigate and observe the effect of cigarette smoke exposure on the quality of spermatozoa of mice. The study was a completely randomized experimental design. The research subject as many as 25 male mice who were randomly divided into 5 groups: group P0 not given exposure, the P1 is given exposure to smoke from 1 bar of cigarette, the P2 is given exposure to smoke from 2 bars of cigarette, the P3 is given exposure to smoke from 3 bars of cigarette and finally the P4 is given exposure to smoke from 4 bars of cigarette. The treatment is given for 30 days. At the end of experiment, results showed a significant difference in the concentration and motility of spermatozoa between the groups exposed to cigarette smoke (p<0,05) compared with the control group. The morphology of spermatozoa group P2,P3,P4, was found substantially different with the control group (p<0,05) but group P1 did not have significant difference in results with the control group. The exposure to cigarette smoke negatively affects sperm quality caused by the free radicals generated by the smoke. Key Word: cigarette smoke, sperm quality.

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Pengaruh Lama Thawing yang Berbeda pada Suhu 25 oC Terhadap Kualitas Semen Beku Sapi Ongole

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.6538 ◽

2019 ◽

Vol 6 (1) ◽

pp. 119

Author(s):

Enike Dwi Kusumawati ◽

Syam Rahadi ◽

Sugeng Santoso ◽

Dyah Lestari Yulianti

Keyword(s):

Sperm Quality ◽

Animal Husbandry ◽

Randomized Design ◽

Frozen Semen ◽

Significant Difference ◽

Completely Randomized Design ◽

Bull Sperm

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh lama thawing yang berbeda pada suhu 250C untuk mendapatkan kualitas spermatozoa semen beku sapi ongole yang optimal. Penelitian dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang. Metode Penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Setiap perlakuan thawing diberikan ulangan sebanyak 10 sampel semen beku sapi ongole. Hasil penelitian menunjukkan bahwa perlakuan dengan lama thawing 7, 15, 30 detik pada suhu 25oC memberikan perbedaan pengaruh yang sangat nyata (p<0.01) pada motilitas, viabilitas dan abnormalitas spermatozoa semen beku sapi ongole. Motilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 40,8%, viabilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 82,39%, sedangkan abnormalitas terendah diperoleh pada perlakuan P3 dengan rata-rata sebesar 11,95%. Berdasarkan penelitian ini maka dapat disimpulkan bahwa thawing pada suhu 25oC dengan lama waktu 30 detik memberikan kualitas spermatozoa yang paling baik sehingga disarankan untuk melakukan thawing pada suhu 25oC dengan lama waktu 30 detik. Kata kunci: abnormalias, mortalitas, ongole, thawing, viabilitas ABSTRACT This study aims to determine the effect of different thawing time at 250C to get the sperm quality of frozen semen ongole bull. Research conducted at the Laboratory of Animal Husbandry Faculty Kanjuruhan University of Malang. The method of study by using Completely Randomized Design (CRD) factorial. Any treatment given repeated thawing of frozen samples of 10 times. The study show that treatment with time thawing 7 (P1), 15 (P2), 30 (P3) seconds at 25oC gives a very significant difference (P<0.01) on motility, viability and abnormalities of ongole Bull sperm. The highest motility and viability were obtained at P3 (40,8%) and (82,39%), while the lowest abnormalities obtained on P3 (11,95%). Based on this research it can be concluded that thawing at 25oC with 30 seconds to give the best quality sperm that is recommended for thawing. Keywords: abnormality, motility, ongole, thawing, viabilit

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EFEK PEMBERIAN L-ARGININ TERHADAP GAMBARAN HISTOLOGI JUMLAH SPERMATOSIT PRIMER PADA MENCIT (Mus musculus) SETELAH TERPAPAR SUHU PANAS

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v8i1.2019.22-25 ◽

2020 ◽

Vol 8 (1) ◽

pp. 22

Author(s):

Dirga Januar Surya Utama ◽

Suherni Susilowati ◽

Tatik Hernawati ◽

Erma Safitri ◽

Sri Mulyati ◽

...

Keyword(s):

Body Weight ◽

Data Analysis ◽

High Temperature ◽

Analysis Of Variance ◽

Adult Male ◽

Mus Musculus ◽

Primary Spermatocyte ◽

Male Mice ◽

Randomized Design ◽

Significant Difference

The purpose of this study was to know the effect of L-arginine on the histology of primary spermatocyte count in mice (Mus musculus) after high temperature exposed. The subjects of this study were 20 adult male mice, 8 weeks old with an body weight range from 20-40 grams. This research conducted by using Complete Randomized Design (RAL) with 4 treatments and 5 replications. The treatments consisted of, P0- = treatment with 1ml of aquabidest without high temperature exposed, P0 + = treatment with 1ml of aquabidest after high temperature exposed for 1hour per-day for 35 days, P1 = 1.3mg / day L-arginine dissolved in 1ml aquabidest and given orally after high temperature exposed for 1hour per-day for 35 days and P2 = 2.6mg / day L-arginine dissolved in 1ml aquabidest given orally after high temperature exposed for 1hour per-day for 35 days. Observations done by making histologic preparations of testicular organs and then calculated the total number of primary spermatocyte per treatment. The data of primary spermatocyte calculated and analyzed by using Analysis of Variance (ANOVA), followed by Duncan test. The result from data analysis showed that there was a significant difference (p<0.05) between P1 and P2 with control,between P1 and P2 the analysis did not show any significant difference (p> 0.05).

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PENGARUH WADAH MEMASAK YANG BERBEDA TERHADAP SIFAT MUTU ORGANOLEPTIK DAGING AYAM BUMBU RINTEK WUUK (RW)

ZOOTEC ◽

10.35792/zot.40.1.2020.26776 ◽

2019 ◽

Vol 40 (1) ◽

pp. 74

Author(s):

Trifonia Kasih ◽

Meilani R. Tinangon ◽

Sofi M. Sembor ◽

Merri D. Rotinsulu

Keyword(s):

Data Analysis ◽

Chicken Meat ◽

Real Difference ◽

Randomized Design ◽

Completely Randomized Design

THE EFFECT OF DIFFERENT COOKING CONTAINER ON THE SENSORIC QUALITY OF TRADITIONAL “RINTEK WUUK” (RW) SEASONING CHICKEN. The purpose of this study was to determine the effect of different cooking containers on the organoleptic qualities of RW seasoning chicken meat. This study used a completely randomized design (CRD) with three treatments and 40 replications (untrained panelists). The treatments were arranged as follows: R1 = bamboo container, R2 = earthen pot container, R3 = pan container. The results and further testing of the doubled duncan region showed that different cooking containers gave a real difference (P˂0.05) to the taste but did not give a difference (P˃0.05) to the color, aroma, and texture, but generally accepted by the panelists. Based on data analysis and discussion for all variables it can be concluded that Rintek Wuuk (RW) seasoned chicken meat cooked using bamboo containers is preferred. Keywords: Cooking container, Chicken meat, RW seasoning, Organoleptic

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Karakteristik dan kualitas semen sapi Sumba Ongole dalam pengencer BTS yang dimodifikasi dengan susu kedelai

Jurnal Sains dan Teknologi Peternakan ◽

10.31605/jstp.v2i2.1037 ◽

2021 ◽

Vol 2 (2) ◽

pp. 64-73

Author(s):

Alphonsius Umbu Jara Lodu ◽

Alexander Kaka ◽

Iven Patu Sirappa

Keyword(s):

Sperm Quality ◽

Sperm Concentration ◽

Spermatozoa Motility ◽

Randomized Design ◽

Semen Volume ◽

Significant Difference ◽

Completely Randomized Design ◽

Live Sperm ◽

Fresh Semen

This study aimed to find out the quality of fresh semen from Sumba Ongole (SO) bulls which was diluted with Bestvile Thawing Solution (BTS) diluent modified with soybean milk (SKD). The study used a completely randomized design consisting of four treatments and 5 replications, P0 (100 % BTS), P1 (95 % BTS + 5 % SM), P2 (90 % BTS + 10 % SM), and P3 (85 % BTS + 15 % SM). The parameters observed were the colour, smell, consistency, pH, volume, motility, viability, and abnormalities of spermatozoa. The results showed that the characteristics were in the normal, creamy white semen colour, semen volume 3,6 ml, medium consistency, pH 6,3, typical SO bulls smell, sperm motility 85 %, mass activity +++, sperm concentration 1276 million/ml, live sperm 90,05 % and sperm abnormalities 6,15 % and quality there was no significant difference between the four treatments. The spermatozoa motility and viability in each treatment was able to which achieved on day 1. The characteristics of the spermatozoa of SO bulls were in the normal category. Meanwhile, BTS diluent with the addition of SKD was unable to maintain sperm quality.

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Kualitas spermatozoa semen sexing kambing peranakan etawa (pe) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda

Jurnal Sains Peternakan ◽

10.21067/jsp.v5i1.3136 ◽

2017 ◽

Vol 5 (1) ◽

pp. 39-49

Author(s):

Kornelis Tamo Ama ◽

Enike Dwi Kusumawati ◽

Aju Tjatur Nugroho Krisnaningsih

Keyword(s):

Semen Quality ◽

Sperm Quality ◽

Egg White ◽

Laboratory Research ◽

Sedimentation Method ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Fresh Semen

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui kualitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing PE. Metode percobaan yang digunakan adalah percobaan laboratorium dengan menggunakan rancangan acak lengkap (RAL) Setiap perlakuan semen sexing kambing Peranakan Etawa dengan pengencer yang berbeda yaitu (P0) CEP, (P1) CEP+kuning telur+putih telur, (P2)CEP+putih telur (P3), CEP+kuning telur dan diulang sebanyak 10 kali. Variabel yang diukur pada penelitian ini adalah motilitas, viabilitas, dan abnormalitas spermatozoa. Data yang diperoleh dianalisis dengan mengunakan analisis varian. Apabila perlakuan memberikan pengaruh maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa kualitas spermatozoa kambing Peranakan Etawa (PE) dengan berbagai macam pengencer menunjukkan pengaruh yang sangat nyata (P<0,01). Motilitas dan Viabilitas menunjukkan perbedaan yang sangat nyata (P<0,01) pada berbagai pengencer Motilitas terbaik pada beberapa lapisan atas sebesar 65% dan CEP+PT lapisan atas 60% dilanjutkan oleh CEP+KT+PT lapisan bawah 55,9% dan CEP+PT lapisan bawah 55% dilanjutkan lagi oleh CEP+KT lapisan atas 50% dan lapisan bawah 45% dan yang paling terkecil adalah CEP lapisan bawah 40%. Viabilitas spermatozoa dari yang tertinggi yaitu CEP+KT+PT lapisan atas sebesar 69,553% dan CEP+PT lapisan atas sebesar 69,519% dan dilanjutkan dengan CEP+PT lapisan bawah sebesar 65,504% dan CEP+KT+PT lapisan bawah 65,473%, dan dilanjutkan lagi dengan CEP+KT lapisan atas sebesar 60,269% dan lapisan bawah sebesar 53,476% dan yang paling terkecil yaitu CEP lapisan bawah sebesar 40,371%. Presentase Abnormalitas menunjukkan bahwa perlakuan tidak memberikan pengaruh nyata (P>0,05). Tetapi CEP+KT+PT lapisan atas menunjukkan persentase yang paling rendah yaitu sebesar 4,88% dibandingkan perlakuan lainnya. Dari hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer CEP+ kuning telur + putih telur pada lapisan atas memberikan hasil terbaik terhadap kualitas spermatozoa ditinjau dari motilitas, viabilitas dan abnormalitas. ABSTRACT The purpose of this study was to determine the sexing sperm quality of Etawa cross-bred goat (PE) with egg white sedimentation method using different diluents. The material used in this study was the fresh semen Etawa crossbreed goat (PE) of the center for Artificial Insemination (BBIB) Singosari Malang. The method is laboratory research using completely randomized design. Consist of CEP, CEP + KT, CEP + PT, CEP + KT + PT and repeated 10 times. The variables are motility, viability and abnormality of sperm. Data were analyzed by using variance analysis. If the treatment effect then continued by Least Significant Difference (LSD). The results showed that the quality of Etawa cross-bred goat sperm with various diluents showed a significant influence (P <0.01). Motility and viability showed differences (P <0.01) in various diluents. The best motility and viability on top layer of CEP+KT+PT diluent were 65% and 69%, 55%. Percentage abnormalities showed that the treatment was not significant effect (P> 0.05). but top layer CEP+KT+PT diluent shows the percentage of abnormality west 4.88% compared to the other treatments. From the results of this study concluded that the quality of sexing semen quality by using dilution CEP + yolk white egg on the top layer gives the best results on the quality of sperm in terms of motility, viability and abnormalities. Based on this study it is suggested that the use of sexing semen with egg white sedimentation method using diluent CEP + yolk + white egg.

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PENGARUH SKARIFIKASI MENGGUNAKAN URIN KAMBING PADA BIJI GAMAL (Gliricida sepium) DENGAN WAKTU PERENDAMAN BERBEDA TERHADAP KUALITAS BENIH

Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) ◽

10.23960/jrip.2021.5.1.1-7 ◽

2021 ◽

Vol 5 (1) ◽

pp. 1-7

Author(s):

Holil Pathan Nur ◽

Agung Kusuma Wijaya ◽

Liman Liman ◽

Muhtarudin Muhtarudin

Keyword(s):

Data Analysis ◽

Animal Husbandry ◽

Gliricidia Sepium ◽

Animal Nutrition ◽

Soaking Time ◽

Randomized Design ◽

Significant Difference ◽

Hard Seeds ◽

Completely Randomized Design

This study aimed to determine the effect of scarification using goat urine with different soaking time on the quality of gamal seeds (Gliricidia sepium) and determine the best scarification treatment that produces normal sprouts and the best sprouts. This research was conducted in January 2020, located in the Laboratory of Animal Nutrition and Feed, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung. This study used an experimental method using Completely Randomized Design with 5 treatments (control or without soaking, seed soaking for 4, 8, 12, and 16 hours) and 6 replications. Each trial unit received 30 seeds of Gamal. Data analysis was performed using the ANOVA continued by the least significant difference (LSD) test. The results showed that scarification of gamal seeds in goat urine at different times of soaking gave significantly effect (P <0.05) on germination, normal sprouts, dead seeds and hard seeds, but did not affect on abnormal sprouts and yield attacked by pests. The best scarification treatment for normal sprouts and sprouts rate was soaking for 16 hours, namely 60.00% and 47.73%, respectively. This results showed the longer soaking the seeds, the higher the germination and normal sprouts. Keywords: Gamal seeds, Goat urine, Scarification, Soaking time

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Motilitas dan Vabilitas Spermatozoa Ayam Kampung pada Suhu 5°C Menggunakan Pengencer dan Lama Simpan yang Berbeda

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v5i3.7242 ◽

2018 ◽

Vol 5 (2) ◽

pp. 102

Author(s):

Enike Dwi Kusumawati ◽

Selvinus Lawu Woli ◽

Aju Tjatur Nugroho Krisnaningsih ◽

Waluyo Edi Susanto ◽

Syam Rahadi

Keyword(s):

Data Analysis ◽

Storage Temperature ◽

Coconut Water ◽

Laboratory Research ◽

Ringer Lactate ◽

Randomized Design ◽

Native Chicken ◽

Lactate Solution ◽

Better Than

ABSTRAKPenelitian ini dilakukan untuk mengetahui motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5oC menggunakan pengencer dan lama simpan yang berbeda. Metode yang digunakan dalam penelitian ini adalah penelitian laboratorium menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan pengencer ringer lactat solution, air kelapa dan tanpa pengencer serta lama simpan 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, masing-masing diulang 10 kali. Variabel yang diamati yaitu motilitas dan viabilitas spermatozoa. Analisa data yang digunakan adalah analisis varian. Hasil analisis data menunjukkan bahwa motilitas dan viabilitas spermatozoa menggunakan pengencer ringer lactat solution lebih tinggi (P<0,01) serta dapat bertahan sampai lama simpan 24 jam dibandingkan air kelapa dan tanpa pengencer. Adapun nilai motilitas ringer lactat solution, air kelapa dan tanpa pengencer pada lama simpan 24 jam masing-masing sebesar 43,5±17,17%; 8±4,83%; 6,5±2,4%, sedangkan nilai viabilitasnya sebesar 83,2±7,25%; 64,6±3,20%; dan 63,1±2,33%. Kesimpulan dari hasil penelitian ini adalah ringer lactat solution lebih baik dibandingkan air kelapa dan tanpa pengencer dalam mempertahankan kualitas semen ayam kampung pada suhu simpan 5oC sampai lama simpan 24 jam.Kata Kunci : air kelapa, ayam kampung, motilitas, spermatozoa, viabilitas ABSTRACTThis study was conducted to determine the motility and viability of spermatozoa of Native chickens at 5oC using different diluents and time storage. The method used in this study was laboratory research using Factorial Completely Randomized Design with ringer lactate solution, coconut water and without diluent at 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30 hours of time storage each repeated 10 times. The variables observed were motility and viability of sperm. Data analysis used is variance analysis. The results of data analysis showed that the motility and viability of spermatozoa using ringer lactate solution diluents was higher (P <0.05) than coconut water and without diluents. The motility values of ringer lactat solution, coconut water and without diluents were 43,5±17,17%; 8±4,83%; 6,5±2,4% respectively, while the viability values were 83,2±7,25%; 64,6±3,20% and 63,1±2,33%. The conclusion of this study is that ringer lactat solution is better than coconut water an without diluents in maintaining the quality of Native chicken semen at a storage temperature of 5oC until 24 hours.Keywords: coconut water, motility, native chicken, sperm, viability

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Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v7i2.10886 ◽

2020 ◽

Vol 7 (2) ◽

pp. 119

Author(s):

Lukman Affandhy ◽

Muchamad Luthfi ◽

Dian Ratnawati ◽

Frediansyah Firdaus

Keyword(s):

Data Analysis ◽

Analysis Of Variance ◽

Research Method ◽

Moringa Oleifera ◽

The Third ◽

Randomized Design ◽

Leaf Powder ◽

Completely Randomized Design ◽

Second Period

ABSTRAK Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci: kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

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