scholarly journals EVALUATION OF TRAIT VARIABILITY IN VARIOUS COLLECTION SAMPLES OF ALFALFA

2021 ◽  
Vol 2021 (1) ◽  
pp. 15-23
Author(s):  
Mikhail Makarenkov ◽  
Nikolay Kozlov ◽  
Tamara Komkova

The research was carried out in the field at the Central Experimental Base of the Federal Williams Research Center of Forage Production and Agroecology. The object of research was 41 samples of alfalfa. The samples were sown according to the scheme of a standard collection nursery. All studies and observations were carried out on the herbage of the second and third years of life. Statistical processing of the research results showed a generally high level of variation in the characteristics. We identified the 4 best samples for a set of features. Several significant positive and negative correlations have been identified, which can be used as indirect signs in the evaluation and selection of promising forms. This method allowed us to identify 3 genetically different groups (clusters) of samples that differ in the nature of the formation of traits. The first cluster includes samples 49, 54, 66, ... and 193 belonging to various studied species. But mostly they represent the species Medicago sativa L. The samples differ little in the content of crude fiber and crude protein. The second cluster includes samples 454, 605, 583, ... and 598, which had average values for most indicators. The third group of samples includes 192, 646 and 355 (Medicago lupulina L. and Medicago glutinosa M.B.) with a maximum content of crude protein. The analysis of the distance and kinship between the samples revealed the presence of three clusters that can be used in breeding for combinational ability and consolidation of breeding-valuable traits.

Author(s):  
Nikolay Malyuzhenets ◽  
Nikolay Kozlov ◽  
Evgenia Malyuzhenets ◽  
Tatyana Kozlova

The research was carried out in the field at the Central experimental base of the «Federal Williams Research Center» The object of research were 32 samples of bluegrass. Samples are sown according to the scheme of a standard collection nursery. All studies and observations were carried out on the herbage of the 4th year of life. Statistical processing of the research results revealed the average level of variation of signs. Several reliable correlation dependences have been revealed, which can be used as indirect signs in the evaluation and selection of promising breeding material. Multivariate analysis showed that in the bluegrass sample, there are 4 clusters (genetically heterogeneous groups), which can be used for selection on the combinational ability and consolidation of selection — valuable traits and properties. Samples of Kentucky (USA) and Balin (Germany) had the maximum superiority (distance), when paired comparison of Euclidean distances. The use of these samples with a high probability promises to obtain perspective breeding material.


2002 ◽  
Vol 53 (6) ◽  
pp. 629 ◽  
Author(s):  
J. M. Musial ◽  
K. E. Basford ◽  
J. A. G. Irwin

Lucerne (Medicago sativa L.) is autotetraploid, and predominantly allogamous. This complex breeding structure maximises the genetic diversity within lucerne populations making it difficult to genetically discriminate between populations. The objective of this study was to evaluate the level of random genetic diversity within and between a selection of Australian-grown lucerne cultivars, with tetraploid M. falcata included as a possible divergent control source. This diversity was evaluated using random amplified polymorphic DNA (RAPDs). Nineteen plants from each of 10 cultivars were analysed. Using 11 RAPD primers, 96 polymorphic bands were scored as present or absent across the 190 individuals. Genetic similarity estimates (GSEs) of all pair-wise comparisons were calculated from these data. Mean GSEs within cultivars ranged from 0.43 to 0.51. Cultivar Venus (0.43) had the highest level of intra-population genetic diversity and cultivar Sequel HR (0.51) had the lowest level of intra-population genetic diversity. Mean GSEs between cultivars ranged from 0.31 to 0.49, which overlapped with values obtained for within-cultivar GSE, thus not allowing separation of the cultivars. The high level of intra- and inter-population diversity that was detected is most likely due to the breeding of synthetic cultivars using parents derived from a number of diverse sources. Cultivar-specific polymorphisms were only identified in the M. falcata source, which like M. sativa, is outcrossing and autotetraploid. From a cluster analysis and a principal components analysis, it was clear that M. falcata was distinct from the other cultivars. The results indicate that the M. falcata accession tested has not been widely used in Australian lucerne breeding programs, and offers a means of introducing new genetic diversity into the lucerne gene pool. This provides a means of maximising heterozygosity, which is essential to maximising productivity in lucerne.


Author(s):  
Valentina Korovina ◽  
Nikolay Kozlov ◽  
Tamara Komkova

The research was carried out in the field at the Central experimental base of the Federal Williams Research Center. The object of research was 13 samples of timothy-grass. Samples were sown according to the scheme of a standard collection nursery. All studies and observations were carried out on the herbage of the 2nd and 3rd year of life. Statistical processing of the research results indicates a low level of variation. The sample № 414 stood out high content of raw protein and raw fat. Two significant correlations were found (between the content of phosphorus and potassium, as well as between crude fiber and crude ash). Analysis of remoteness and kinship between samples showed the presence of 5 clusters (genetically different quality groups), which can be used for breeding for combinational ability and fixing of breeding-valuable features and properties.


2020 ◽  
Vol 2020 (4) ◽  
pp. 39-45
Author(s):  
Valentina Korovina ◽  
Nikolay Kozlov ◽  
Tamara Komkova

The research was carried out in the field at the Central experimental base of the Federal Williams Re¬search Center. The object of research was 12 samples of wild-growing meadow timothy grass. Samples were sown according to the scheme of a standard collection nursery. All studies and observations were carried out on the herbage of the 2nd and 3rd year of life. Statistical processing of results of researches of meadow timothy indicates a low level of variation in the main morphological and physiological characte¬ristics. Only by the number of vegetative shoots have the coefficient of variation reach 35.8%. Samples 416 and 419 were distinguished by precocity, samples 406 and 419 by the height of plants during flower¬ing, sample 412 by the number of generative shoots, and sample 412 by the yield of air-dry mass. Sam¬ples 409 and 414 were distinguished by the length of the sultan, and the weight of seeds — 414 and 415. Nine reliable correlations were found at the significance level 05 and 01. However, all of them are related to the yield of the number of generative and vegetative shoots. Using the binomial equation, the experi¬mentally obtained variability of dry mass yield from the number of generative and vegetative shoots by 93.1% is described. The optimal productivity of meadow timothy grass stand for generative shoots in the area of 550–600 shoots, and vegetative ones — in the area of 320–350 shoots per square meter. Analysis of remoteness and kinship between samples showed the presence of 2 clusters (genetically different quality groups), which can be used for combinational ability selection and fixing of selection-valuable features and properties.


1987 ◽  
Vol 67 (3) ◽  
pp. 735-746 ◽  
Author(s):  
P. GERVAIS ◽  
J. M. GIRARD

A 3-yr study on height and frequency of cutting showed that alfalfa (Medicago sativa L.) cut at 5 cm from the soil produced significantly more dry matter per hectare than that harvested at 10 cm, but the differences between the two heights decreased with the years and the frequency of cutting. Forages cut at either height had similar mineral composition at any one cutting. However, the percentages of cell walls and of lignocellulose were, in general, highest in alfalfa cut at 5 cm. Height of cutting did not affect the stand nor the accumulation of food reserves in the roots in the fall. The two-cut system gave a uniform production from year to year and maintained the stand at a high level. The three-cut system, the last cut taken in October, yielded the highest yields of dry matter and of crude protein while the four-cut system produced the least and had the thinnest stand at the end of the experiment. Frequency of cutting gave rise to significant variations in the chemical composition of the forages, variations resulting from the difference in the stages of growth at harvest. In general, alfalfa cut at a younger stage exhibited a more valuable composition than that harvested at a later stage. Food storage in the roots varied with frequencies, being highest with the two-cut system and lowest with the four-cut system.Key words: Alfalfa, height and frequency of cutting, yield, chemical composition, food reserves


Author(s):  
Sri G. Thrumurthy ◽  
Tania Samantha De Silva ◽  
Zia Moinuddin ◽  
Stuart Enoch

Specifically designed to help candidates revise for the MRCS exam, this book features 350 Single Best Answer multiple choice questions, covering the whole syllabus. Containing everything candidates need to pass the MRCS Part A SBA section of the exam, it focuses intensively on the application of basic sciences (applied surgical anatomy, physiology, and pathology) to the management of surgical patients. The high level of detail included within the questions and their explanations allows effective self-assessment of knowledge and quick identification of key areas requiring further attention. Varying approaches to Single Best Answer multiple choice questions are used, giving effective exam practice and guidance through revision and exam technique. This includes clinical case questions, 'positively-worded' questions, requiring selection of the most appropriate of relatively correct answers; 'two-step' or 'double-jump' questions, requiring several cognitive steps to arrive at the correct answer; as well as 'factual recall' questions, prompting basic recall of facts.


2021 ◽  
Vol 13 (15) ◽  
pp. 8247
Author(s):  
Dimitrios N. Vlachostergios ◽  
Christos Noulas ◽  
Anastasia Kargiotidou ◽  
Dimitrios Baxevanos ◽  
Evangelia Tigka ◽  
...  

Lentil is a versatile and profitable pulse crop with high nutritional food and feed values. The objectives of the study were to determine suitable locations for high yield and quality in terms of production and/or breeding, and to identify promising genotypes. For this reason, five lentil genotypes were evaluated in a multi-location network consisting of ten diverse sites for two consecutive growing seasons, for seed yield (SY), other agronomic traits, crude protein (CP), cooking time (CT) and crude protein yield (CPY). A significant diversification and specialization of the locations was identified with regards to SY, CP, CT and CPY. Different locations showed optimal values for each trait. Locations E4 and E3, followed by E10, were “ideal” for SY; locations E1, E3 and E7 were ideal for high CP; and the “ideal” locations for CT were E3 and E5, followed by E2. Therefore, the scope of the cultivation determined the optimum locations for lentil cultivation. The GGE-biplot analysis revealed different discriminating abilities and representativeness among the locations for the identification of the most productive and stable genotypes. Location E3 (Orestiada, Region of Thrace) was recognized as being optimal for lentil breeding, as it was the “ideal” or close to “ideal” for the selection of superior genotypes for SY, CP, CT and CPY. Adaptable genotypes (cv. Dimitra, Samos) showed a high SY along with excellent values for CP, CT and CPY, and are suggested either for cultivation in many regions or to be exploited in breeding programs.


2021 ◽  
Vol 14 (7) ◽  
pp. 603
Author(s):  
Vanesa Pérez-Laguna ◽  
Isabel García-Luque ◽  
Sofía Ballesta ◽  
Antonio Rezusta ◽  
Yolanda Gilaberte

The present review covers combination approaches of antimicrobial photodynamic therapy (aPDT) plus antibiotics or antifungals to attack bacteria and fungi in vitro (both planktonic and biofilm forms) focused on those microorganisms that cause infections in skin and soft tissues. The combination can prevent failure in the fight against these microorganisms: antimicrobial drugs can increase the susceptibility of microorganisms to aPDT and prevent the possibility of regrowth of those that were not inactivated during the irradiation; meanwhile, aPDT is effective regardless of the resistance pattern of the strain and their use does not contribute to the selection of antimicrobial resistance. Additive or synergistic antimicrobial effects in vitro are evaluated and the best combinations are presented. The use of combined treatment of aPDT with antimicrobials could help overcome the difficulty of fighting high level of resistance microorganisms and, as it is a multi-target approach, it could make the selection of resistant microorganisms more difficult.


Horticulturae ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 38
Author(s):  
Naji K. Al-Mefleh ◽  
Yahia A. Othman ◽  
Maher J. Tadros ◽  
Amani Al-Assaf ◽  
Samer Talozi

This study investigated the influence of treated greywater on growth and protein content of multipurpose (forage and ornamental) transplants, Prosopis juliflora L., Prosopis tamarugo L., and Albizia lebbeck L. Transplants of tested species were irrigated with treated greywater, diluted greywater (grey + distilled water, 1:1/by volume), and distilled water (control) for seven months. Water quality analysis showed that the concentrations of nutrients and heavy metals found in the greywater were within the acceptable range compared with Jordan Institution for Standard and Metrology (JISM) and the World Health Organization (WHO) thresholds for safe use of greywater. Escherichia coli found in the greywater were lower compared to JISM and WHO guidelines for the safe use of greywater. Irrigation with treated greywater increased shoot fresh weight by 24–39% and dry weight by 34–40% compared to diluted greywater and control. No significant difference in crude protein was noticed between water treatments. Prosopis species (P. juliflora Albizia lebbeck L. and P. tamarugo Albizia lebbeck L.) had higher shoot fresh (35%) and dry weight the same species had lower crude protein (44%) when compared to Albizia lebbeck Albizia lebbeck L. The reuse of treated greywater for landscaping or forage production alleviates the demand for water resources and reduces the pressure on wastewater treatment plants. However, considering the controversial findings of previous studies on greywater quality (especially, long-term reuse), the reuse of treated greywater needs to be considered with caution and periodic quality analyses and economic assessments are required.


Author(s):  
В. Волошин ◽  
Н. Морозков

По результатам научных исследований, выполненных за ряд лет в растениеводстве и животноводстве, показана целесообразность интродукции в кормопроизводство Пермского края новой многолетней бобовой культуры эспарцета песчаного. По четырёхлетним наблюдениям (2012 2015 годы) в полевых опытах культура не уступала по урожайности кормовой массы традиционному для региона клеверу луговому, а по концентрации обменной энергии и сырого протеина в сухом веществе была на уровне клевера лугового и люцерны изменчивой, но в разы превосходила их по содержанию сахара. Сенаж это единственный вид зимнего корма, максимально сохраняющий обменную энергию, протеин, сахар, каротин, достаточно концентрированный, чтобы обеспечивать потребности высокопродуктивных животных. В 2018 году на Лобановском молочном комплексе ООО Русь в Пермском районе Пермского края были проведены научнохозяйственный и физиологический опыты по скармливанию сенажа из эспарцета песчаного голштинизированным коровам чёрнопёстрой породы. При использовании сенажа из эспарцета песчаного животные в сутки потребляли сухого вещества на 2,3 3,9 кг на голову больше по сравнению с группой, где в рационе было сено (контроль). В результате использования сенажа из эспарцета песчаного переваримость сухого вещества рациона у опытных групп животных была выше на 2,55 5,80 по сравнению с контрольной органического вещества на 2,54 4,14 . За учётный период научнохозяйственного опыта (120 дней) от коров опытных групп получено по 399327,2 и 414431,9 кг молока, что со средней степенью достоверности выше продуктивности животных контрольной группы (391045,4). По содержанию молочного жира и молочного белка преимущество достоверно также было за опытными группами. Затраты корма на 1 кг молока составили в контроле 0,74 энергетических корм. ед. (ЭКЕ), в опытных группах 0,71 и 0,69 ЭКЕ. Hungarian sainfoin was shown to be an effective perennial legume crop in the forage production of the Perm Territory. For 4 years (2012 2015) this crop produced forage mass, exchange energy and crude protein comparable to the ones of red clover and bastard alfalfa but exceeded them significantly in carbohydrate content. Haylage is the only type of winter forage that has sufficient concentrations of exchange energy, protein, carbohydrates and carotene, satisfying needs of highproductive livestock in nutrients. Haylage from Hungarian sainfoin was fed to Holstein BlackandWhite cows in the frame of experiments conducted in 2018. Cows consumed 2.3 3.9 kg more of dry matter (DM) with the haylage, compared to hay (control). Digestibilities of DM and organic matter were 2.55 5.80 and 2.54 4.14 higher than in the control group, respectively. For 120 days the experimental groups produced 3993 27.2 and 4144 31.9 kg of milk versus 3910 45.4 kg of the control one. The former also exceeded the latter in milk fat and protein contents. Fodder costs per 1 kg of milk amounted to 0.74 energy feed units in the control and 0.71 and 0.69 energy feed units in the experimental groups.


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