scholarly journals Acute Cold Water-Immersion Restraint Stress Induces Intestinal Injury and Reduces the Diversity of Gut Microbiota in Mice

2021 ◽  
Vol 11 ◽  
Author(s):  
Yuan Zhang ◽  
Shuwen Wu ◽  
Yongming Liu ◽  
Jingchang Ma ◽  
Wenpeng Li ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Cold Water ◽  
Water Immersion ◽  
Periodic Acid ◽  
Cold Water Immersion ◽  
Intestinal Injury ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Control Group ◽  
Intestinal Damage

Growing evidence has demonstrated that stress triggers gastrointestinal (GI) disorders. This study aimed to investigate how the acute cold water-immersion restraint (CWIR) stress affects intestinal injury and gut microbiota (GM) distribution. Male C57BL/6 mice were used to establish a CWIR animal model. Hematoxylin–eosin and periodic acid–Schiff staining were performed to assess intestinal histopathological changes. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis and immunofluorescence staining were used to evaluate the expression of inflammatory cytokines and immune cell infiltration in the intestinal tissues. The gut permeability and intestinal occludin protein expression were determined through fluorescein isothiocyanate-dextran detection and western blot, respectively. GM profiles were analyzed via high-throughput sequencing of the fecal bacterial 16S rRNA genes. Results showed that CWIR induced more severe intestinal mucosal injury compared to the control, leading to a significant increase in tumor necrosis factor-α expression, but no infiltration of neutrophil and T cells. CWIR also resulted in GI disruption and increased the permeability of the intestinal mucosa. GM profiles showed that CWIR reduced GM diversity of mice compared with the control group. Specifically, aerobic and gram-negative bacteria significantly increased after CWIR, which was associated with the severity of gut injury under stress. Therefore, acute CWIR leads to severe intestinal damage with inflammation and disrupts the GM homeostasis, contributing to decreased GM diversity. Our findings provide the theoretical basis for the further treatment of intestinal disorders induced by CWIR.

Tolerance of Gaspé fishermen to cold water

1960 ◽  
Vol 15 (6) ◽  
pp. 1031-1034 ◽  
Author(s):  
Jacques LeBlanc ◽  
J. A. Hildes ◽  
O. Héroux
Keyword(s):  
Cold Water ◽  
Pressor Response ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Control Group ◽  
Skin Thickness ◽  
Finger Temperature ◽  
Control Subjects ◽  
Skin Biopsies ◽  
And Control

A group of Gaspé fishermen used to cold water immersion and control subjects from the same vicinity were studied to determine if the fishermen's hands were adapted to cold. With one hand immersed in cold water, the pressor response was greater in the control subjects; the fishermen maintained a higher finger temperature and complained less of pain; heat flow from the fishermen's hands was greater than in the control group; finger numbness as measured by a modification of Mackworth's V-test was variable and not significantly different in the two groups. Skin biopsies showed no difference in skin thickness or cell size but there was a significantly greater number of mast cells in the fishermen's skin. The differences between the fishermen and the control subjects may be related to repeated cold exposure. Submitted on June 7, 1960

Cold Water Immersion Directly and Mediated by Alleviated Pain to Promote Quality of Life in Indonesian with Gout Arthritis: A Community-based Randomized Controlled Trial

2022 ◽  
pp. 109980042110635
Author(s):  
Maria Dyah Kurniasari ◽  
Karen A. Monsen ◽  
Shuen Fu Weng ◽  
Chyn Yng Yang ◽  
Hsiu Ting Tsai
Keyword(s):  
Quality Of Life ◽  
Physical Activity ◽  
Cold Water ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Control Group ◽  
Joint Mobility ◽  
Community Based ◽  
Anxiety Depression

Background: Gout arthritis is an autoinflammatory arthritis that generates chronic long-term pain. Pain impacts physical activities, joint mobility, stress, anxiety, depression, and quality of life. Cold-water immersion therapy reduces inflammation and pain associated with gout arthritis. However, cold-water immersion therapy has not been conducted among people worldwide with gout arthritis. Objective: To investigate the cold-water immersion intervention on pain, joint mobility, physical activity, stress, anxiety, depression, and quality of life among acute gout patients. Methods: A community-based randomized control trial design with two parallel-intervention groups: a cold-water immersion group (20–30°C 20 minutes/day for 4 weeks) and a control group. In total, 76 eligible participants in Tomohon City, Indonesia, were recruited using a multi-stage sampling method and were randomly assigned using block randomization. A generalized estimating equation model was used to analyze the results (coef. β) and produce 95% confidence intervals (CIs). A path analysis was used to analyze mediating effects. Results: Significant pain alleviation ( β = −2.06; −2.42), improved joint mobility ( β = 1.20, 1.44), physical activity ( β = 2.05, .59), stress ( β = −1.25; −1.35), anxiety ( β = −.62; −1.37), and quality of life ( β = 5.34; 9.93) were detected after cold-water immersion at the second-week, and were maintained to the fourth-week time point, compared to pre-intervention and the control group. Depression ( β = −1.80) had decreased by the fourth week compared to the pre-test and control group. Cold-water immersion directly mediated alleviation of pain ( β = −.46, p ≤ .001) and to promote the quality of life ( β = .16, p = .01). Conclusions: Cold-water immersion decreased pain, stress, anxiety, and depression, and increased joint mobility, physical activity, and quality of life. It mediated alleviation of pain to increase the quality of life.

scholarly journals The effects of tow protocol cold water immersion on the post match recovery and physical performance in well-trained handball players

2019 ◽  
Vol 23 (6) ◽  
pp. 288-295
Author(s):  
M. Mokhtar ◽  
B. Adel ◽  
B. Wahib ◽  
A. Hocine ◽  
B. Othman ◽  
...  
Keyword(s):  
Pain Intensity ◽  
Cold Water ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Control Group ◽  
Countermovement Jump ◽  
Intense Training ◽  
Exercise Induced ◽  
High Level ◽  
Experimental Group

Purpose: The purpose of this study is to compare two cold water immersion (CWI) protocols, continuous and fractionated, to optimize the recovery of Handball players after on recovery from exercise resulting in exercise-induced muscle damage. Material: Ten male Handball players (age: 15 ± 1.4 years, mass index: 67.2 ± 5.1 kg, height: 176.6 ± 7.30) voluntarily participated in the study. After three 90-minute training sessions (average heart rate 160 ± 15.81, 156 ± 5.53 and 156 ± 12.24 bpm) per week, participants were divided into 03 groups. The first experimental group (GE1) in continuous immersion (CWIC) of (12 minutes, 12± 0.4° C), a second experimental group (GE2) in fractional immersion (CWIF) of (4 x 2 min at 12 ± 0.4° C + 1 min out of water) and a control group (GC) in passive recovery. Body mass indices (BMI), countermovement (Countermovement jump) and muscle pain (Intensity of pain in the thighs) were measured. Results : The results concerning the percentage differences in the variation of the CMJ occurred respectively at 24h (Z = 12.62, p = 0.004) and 48h (Z = 16.22, p <0.001) compared to the control group. In addition, the results for muscle volume did not report any significant interaction (F (5.64) = 3.42, p = 0.078). The results of both protocols showed their effectiveness in reducing pain intensity by 24 and 48 hours after intense training (F (3.54) = 2.91, p = 0.016, p2 = 0.24). Conclusion: In conclusion, continuous and fractionated cold water immersion is beneficial for neuromuscular recovery 24 hours after intense exercise. The results also demonstrate a rapid recovery of handball players from their physical potential required in high level competitions.

scholarly journals Effect of cold water immersion on muscle damage indexes after simulated soccer training in young soccer players

2020 ◽  
Vol 12 (1) ◽  
pp. 236-241
Author(s):  
Saman Khakpoor Roonkiani ◽  
Mohsen Ebrahimi ◽  
Ali Shamsi Majelan
Keyword(s):  
Body Mass Index ◽  
Muscle Damage ◽  
Body Mass ◽  
Cold Water ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Soccer Players ◽  
Control Group ◽  
Significant Difference ◽  
Soccer Training

Summary Study aim: To investigate the effect of cold water immersion (CWI) on muscle damage indexes after simulated soccer activity in young soccer players. Material and methods: Eighteen professional male soccer players were randomly divided into two groups: CWI (n = 10, age 19.3 ± 0.5, body mass index 22.2 ± 1.3) and control (n = 8, age 19.4 ± 0.8, body mass index 21.7 ± 1.5). Both groups performed a simulated 90-minute soccer-specific aerobic field test (SAFT90). Then, the CWI group subjects immersed themselves for 10 minutes in 8°C water, while the control group subjects sat passively for the same time period. Blood samples were taken before, immediately after, 10 minutes, 24 hours and 48 hours after the training session in a fasted state. Blood lactate, creatine kinase (CK) and lactate dehydrogenase (LDH) enzyme levels were measured. Results: Lactate, CK and LDH levels increased significantly after training (p < 0.001). There were significant interactions between groups and subsequent measurements for CK (p = 0.0012) and LDH (p = 0.0471). There was no significant difference in lactate level between the two groups at any aforementioned time. Conclusion: It seems that CWI after simulated 90-minute soccer training can reduce the values of muscle damage indexes in soccer players.

Influencia del masaje ZNAR y la inmersión en agua fría en el proceso inflamatorio, Creatin Kinasa y percepción al dolor muscular en jugadores de voleibol (Influence of ZNAR massage and cold water immersion on the inflammatory process, Creatine Kinase and

Retos ◽  
2021 ◽  
Vol 44 ◽  
pp. 95-102
Author(s):  
Zeltzin Nereyda Alonso Ramos ◽  
Blanca Rocío Rangel Colmenero ◽  
Myriam Zarai García Dávila ◽  
Gerardo Enrique Muñoz Maldonado ◽  
José Raul Hoyos Flores ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Muscle Damage ◽  
Muscle Pain ◽  
Inflammatory Process ◽  
Cold Water ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Tnf Α

  Las estrategias que aminoren el dolor, la inflamación y el daño muscular provocados por la actividad física de alta intensidad en atletas son de interés en la recuperación deportiva, por lo que el objetivo del estudio fue conocer el efecto del masaje ZNAR y la inmersión en agua fría a 10° sobre el proceso inflamatorio a través de la interleucina 6 (IL-6), interleucina 10 (IL-10), el factor de necrosis tumoral Alpha (TNF-α), el daño muscular mediante la Creatin Kinasa (CK) y la percepción al dolor muscular a través de la escala visual análoga de dolor (EVA) en jugadores de voleibol. Participaron 19 atletas divididos en un grupo control y un grupo experimental, sometidos a dos protocolos de recuperación (masaje ZNAR e inmersión en agua fría) posterior a un test de inducción a la fatiga. Se cuantifico la IL-6, IL-10, TNF, CK y EVA. Los resultados mostraron cambios significativos (p < .05) en las tomas de recuperación en el comportamiento del proceso inflamatorio, la CK y la percepción al dolor muscular con ambos métodos de recuperación. Conclusión, el Masaje ZNAR favorece a la recuperación de la IL-6 y la IL-10 además de la disminución de la CK y la percepción al dolor muscular.  Abstract: The strategies that reduce pain, inflammation and muscle damage caused by high intensity activity in athletes are of interest in sports recovery, the objective of the study was to know the effect of ZNAR massage and cold water immersion at 10 ° on the inflammatory process through interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor Alpha (TNF-α), muscle damage through Creatine Kinase (CK) and the perception of muscle pain through the visual analoge scale (VAS) in volleyball players. 19 athletes were divided into a control group and an experimental group, submitted to two recovery protocols (ZNAR massage and cold water immersion) after a fatigue induction test. IL-6, IL-10, TNF, CK and EVA were quantified. The results showed significant changes (p < .05) in the recovery shots in the behavior of the inflammatory process, the CK and the perception of muscle pain with both recovery methods. Conclusion, the ZNAR Massage favors the recovery of IL-6 and IL-10 in addition to the decrease in CK and the perception of muscle pain

Hypothermic facilitation of retrograde V-A conduction

1960 ◽  
Vol 15 (6) ◽  
pp. 1035-1040 ◽  
Author(s):  
Carolyn K. Francis ◽  
Carol A. Campbell ◽  
H. E. Hoff
Keyword(s):  
Cold Water ◽  
Pressor Response ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Control Group ◽  
Skin Thickness ◽  
Finger Temperature ◽  
Control Subjects ◽  
Skin Biopsies ◽  
And Control

A group of Gaspé fishermen used to cold water immersion and control subjects from the same vicinity were studied to determine if the fishermen's hands were adapted to cold. With one hand immersed in cold water, the pressor response was greater in the control subjects; the fishermen maintained a higher finger temperature and complained less of pain; heat flow from the fishermen's hands was greater than in the control group; finger numbness as measured by a modification of Mackworth' V-test was variable and not significantly different in the two groups. Skin biopsies showed no difference in skin thickness or cell size but there was a significantly greater number of mast cells in the fishermen's skin. The differences between the fishermen and the control subjects may be related to repeated cold exposure. Submitted on June 7, 1960

Effects of Pre- and Post-Exercise Cold-Water Immersion Therapy on Passive Muscle Stiffness

2019 ◽  
Vol 34 (02) ◽  
pp. 72-78 ◽  
Author(s):  
Moritz Hüttel ◽  
Tobias Golditz ◽  
Isabel Mayer ◽  
Rafael Heiss ◽  
Christoph Lutter ◽  
...  
Keyword(s):  
Cold Water ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Muscle Stiffness ◽  
Control Group ◽  
Group Exercise ◽  
Post Exercise ◽  
Group A ◽  
Set Up

Abstract Background Cold-water immersion (CWI) has become a popular preventive, regenerative and performance-enhancing intervention in various sports. However, its effects on soft tissue, including changes of intramuscular stiffness, are poorly understood. The purpose of this study was to investigate the effect of CWI on muscle stiffness. Patients/Material and Methods Thirty healthy participants were included and divided into the three following groups (n = 10): 1) post-ESU group: exercise and CWI (post-exercise set-up); 2) control group: exercise without CWI (control condition); 3) pre-ESU group: CWI alone (pre-exercise set-up). Acoustic radiation force impulse (ARFI) elastography was conducted to assess tissue stiffness (shear wave velocity, SWV). Values obtained at resting conditions (baseline, t0) were compared to values post-exercise (t1, for post-ESU group and control group), post-CWI (t2, for post-ESU group and pre-ESU group; rest for control group) and to 60-min follow-up time (t3, for all groups). Data were assessed in superficial and deep muscle tissue (rectus femoris muscle, RF; vastus intermedius muscle, VI). Results For the post-ESU group (CWI post-exercise), there was no significant difference between the time points of measurements: exercise (t1: RF: 1.63 m/s; VI: 1.54 m/s), CWI (t2: RF: 1.63 m/s; VI: 1.53 m/s) and at 60-min follow-up (t3: RF: 1.72 m/s; VI: 1.61 m/s). In the control group, a significant decrease of SWV was found between baseline conditions at t0 and post-exercise (t1) at VI (VI: 1.37 m/s; p = 0.004; RF: 1.59 m/s; p = 0.084). For t2 and t3, no further significant changes were detected. Regarding the pre-exercise set-up (pre-ESU group), a significant decrease in SWV from baseline to t2 in VI (1.60 m/s to 1.49 m/s; VI: p = 0.027) was found. Conclusion This study shows varying influences of CWI on muscle stiffness. Overall, we did not detect any significant effects of CWI on muscle stiffness post-exercise. Muscle stiffness-related effects of CWI differ in the context of a pre- or post-exercise condition and have to be considered in the implementation of CWI to ensure its potential preventive and regenerative benefits.

scholarly journals Cold-Water Immersion Promotes Antioxidant Enzyme Activation in Elite Taekwondo Athletes

2021 ◽  
Vol 11 (6) ◽  
pp. 2855
Author(s):  
Eun-Hee Park ◽  
Seung-Wook Choi ◽  
Yoon-Kwon Yang
Keyword(s):  
Antioxidant Enzymes ◽  
Cold Water ◽  
Water Immersion ◽  
Lipid Peroxides ◽  
Cross Sectional Study ◽  
Cold Water Immersion ◽  
Enzyme Activation ◽  
Control Group ◽  
Cross Sectional ◽  
Significant Difference

The aim of this study was to investigate the effect of cold-water immersion (CWI) on lipid peroxides and antioxidant enzymes in adult Taekwondo athletes after a match. A cross-sectional study was performed. After a Taekwondo match, the control group remained seated passively, while the treatment group immersed their legs below the knee joint in cold water at 10 °C. Blood samples were taken at pre-match, post-match, post-treatment, and post-rest, and changes in malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) concentrations were analyzed. The results showed that there was a significant difference in MDA between the two groups, and while the CWI group had 19% lower SOD concentration compared to the control group, and the difference was not significant. However, in case of interaction for GPx concentration (p < 0.001), a statistically significant difference was found between the two groups (p < 0.05). In conclusion, CWI after a Taekwondo match elevates the concentration of antioxidant enzymes.

scholarly journals The The Effects of Cold Water Immersion on Anaerobic Power, Dynamic Balance and Muscle Activation After a karate kumite fighting in Female Karateka

2017 ◽  
Vol 6 (3) ◽  
pp. 72-79
Author(s):  
Taher Afshar Nezhad ◽  
Samane Faghihi ◽  
Amir Hazrati ◽  
Khadije Bahrami
Keyword(s):  
Muscle Activation ◽  
Cold Water ◽  
Dynamic Balance ◽  
Water Immersion ◽  
Anaerobic Power ◽  
Cold Water Immersion ◽  
Control Group ◽  
Anaerobic Performance ◽  
Post Intervention ◽  
Significant Difference

Many athletes are using specific techniques to minimize fatigue and accelerate recovery processes. Cold water immersion (CWI) is one of the most popular interventions used by athletes to potentially return to their pre-fatigue performance level. the purpose of this study was to investigate the effects of CWI on anaerobic performance, balance and muscle activation of female karateka after a simulated match. 15 young female karateka (age: 18.7±1.7 years, body mass: 55.5±6.3 kg, height:165±5.1 cm) with at least three years' experience in karate kumite fighting were included in the study. After three round 3-minute competition, participants were grouped into a CWI group (20 min at 12±1°C) and a control group (CTL). Anaerobic power (30 s Wingate test), and dynamic balance (Star-Excursion test) were measured before the competition and 24 h after intervention. Surface electromyography (EMG) was sampled from quadriceps femoris muscles. Peak normalized muscle activation levels and force were identified during maximal isometric test. A significant decrease in the anaerobic performance after the competition was observed for both groups (p<0.05). CWI were effective in enhancing the anaerobic performance after competition compared with the CTL. Dynamic balance decreased for two groups, although CWI resulted in the smallest reduction in balance. There was a significant difference in peak and mean RMS values of the EMG in Rectus Femoris but not Vastus muscles after the CWI intervention when compared to CTL (p<0.05). CWI improve recovery related to dynamic balance and anaerobic performance of karate kumite fighter. It can be concluded that CWI appears to promote muscle activation and reduce fatigue that is related to better performance in 24 hours post intervention.

scholarly journals Characteristics of gut microbiota in people with obesity

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255446
Author(s):  
Mengmeng Duan ◽  
Yuezhu Wang ◽  
Qiang Zhang ◽  
Rong Zou ◽  
Min Guo ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Illumina Miseq ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Control Group ◽  
Sequencing Data ◽  
Genome Database ◽  
Control Subjects ◽  
And Control

Background Obesity is the cause of cardiovascular diseases and other diseases, leading to increased medical costs, and causing a great burden to individuals, families and society. The prevalence of obesity is increasing and has become a global health problem. There is growing evidence that gut microbiota plays an important role in obesity. In this article, we revealed the differences in the gut microbiota between 21 people with obesity and 21 control subjects, and predicted the functional potential changes by 16S rRNA sequencing of the fecal bacteria of the subjects. Methods The raw sequencing data of 21 healthy Beijing volunteers was downloaded from Microbial Genome Database System. Microbial 16S rRNA genes of 21 adults with obesity were sequenced on an Illumina MiSeq instrument and analyzed by using bioinformatics and statistical methods. Results The diversity of gut microbiota in people with obesity decreased significantly. There were significant differences in gut microbiota between the Obesity and Control group at different levels. At the phylum level, Firmicutes, Bacteroidetes, Actinobacteria and Fusobacteria are significantly different between the Obesity and Control group. In people with obesity, the ratio of Firmicutes/Bacteroidetes decreased significantly. At the genus level, there were significant differences among the 16 major genera, of which four genera Prevotella, Megamonas, Fusobacterium and Blautia increased significantly in people with obesity, while the remaining 12 genera, Faecalibacterium, Lachnospiracea_incertae_sedis, Gemmiger and Clostridium XlVa, etc. decreased significantly. At the species level, nine species including Bacteroides uniformis and Prevotella copri had significant differences. Compared with the control group, subjects with obesity were abnormalities in 57 pathways, mainly in Carbohydrate metabolism and Lipid metabolism. Conclusions Overall, our study revealed differences in the gut microbiota between people with obesity and control subjects, providing novel target for the treatment of individuals with obesity.

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