scholarly journals Enhancement of Biomass Production in Colony-Forming Green Algae, Botryosphaerella sudetica, Under Mixotrophic Cultivation

2021 ◽  
Vol 12 ◽  
Author(s):  
Hyun-Sik Yun ◽  
Young-Saeng Kim ◽  
Ho-Sung Yoon
Keyword(s):  
Fatty Acids ◽  
Green Algae ◽  
Biomass Production ◽  
Colony Size ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
Biomass Productivity ◽  
Mixotrophic Cultivation ◽  
Bisphosphate Carboxylase ◽  
Lipid Components

In this study, we characterized the potential of colony-forming green algae, Botryosphaerella sudetica KNUA107, isolated from Ulleung Island, South Korea, as a bioresource and analyzed the effects of mixotrophic cultivation on its bioresource production efficiency. Internal transcribed spacer (ITS) (ITS1, 5.8S, and ITS2), ribulose bisphosphate carboxylase large subunit (rbcL), and elongation factor Tu (tufa) regions were used for molecular identification and phylogenetic analysis. B. sudetica KNUA107 had a strong relationship with the green algae of Botryococcus and Botryosphaerella genera, which are colony-forming species, and was also associated with members of the Neochloris genus. To improve biomass productivity, we tested mixotrophic cultivation conditions using several organic carbon sources. Glucose supplementation stimulated B. sudetica KNUA107 growth and reduced the time needed to reach the stationary phase. In addition, the colony size was 1.5–2.0 times larger with glucose than in photoautotrophic cultures, and settleability improved in proportion to colony size. The total lipid content and biomass productivity were also higher in cultures supplemented with glucose. Among the lipid components, saturated fatty acids and monounsaturated fatty acids had the highest proportion. Our study suggests that B. sudetica KNUA107, which has enhanced efficiency in biomass production and lipid components under mixotrophic cultivation, has high potential as a bioresource.

scholarly journals Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1366
Author(s):  
Cristina Soares ◽  
Sara Sousa ◽  
Susana Machado ◽  
Elsa Vieira ◽  
Ana P. Carvalho ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Total Lipid Content ◽  
Monounsaturated Fatty Acids ◽  
North Coast ◽  
Bioactive Lipids ◽  
The North ◽  
Atherogenicity Index ◽  
Polycyclic Aromatic

The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.

scholarly journals Effects of Different Levels of Urea as Nitrogen Source on Chemical Composi- tion of Marine Microalgae Nannochloropsis oculata

2021 ◽  
Vol 36 (1) ◽  
pp. 1-11
Author(s):  
Ali Mahmoud Abougrara
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Chemical Constituents ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
High Growth ◽  
Cost Effective ◽  
Essential Amino Acids ◽  
Nannochloropsis Oculata ◽  
Different Levels

Microalgae breeding media must be cost-effective, enable high growth, meet exact re- quirements and be readily available. The effect of different levels of urea [25, 50, 75, and 100%] in the growth medium on the biochemical constituents (protein, carbohydrates, lipids, fatty acids, and amino acids) of the Nannochloropsis oculata, was assessed compared to the F/2 Guillard standard medium. The obtained results revealed that the chemical constituents of N. oculata were influenced by the different levels of urea. The highest total protein was obtained by A4 medium (100% urea) (26.44%) and A3 medium (75% urea) (25.84%). The maximum percentage of essential amino acids (EAA) (51.54%) was obtained by using the A4 medium (100% urea) as compared to the control (100% F/2). The highest total lipid content was achieved by using the A1 medium (25% urea) pro- ducing (17.33 %) and A4 medium (100% urea) (16.98%). Accordingly, the highest total saturated fatty acids percentage (TSFA) of N. oculata was recorded by the A3 medium. In conclusion, the addition of urea is an excellent policy to increase chemical composition and lipid accumulation. The present study recommended taming results for aquaculture feeding through using the proposed A1 medium as a lipid promoter or A4 medium as a protein promoter.

Introduction of macroalgae as a source of biodiesel in Iran: analysis of total lipid content, fatty acid and biodiesel indices

2021 ◽  
pp. 1-8
Author(s):  
Zahra Zarei Jeliani ◽  
Nasrin Fazelian ◽  
Morteza Yousefzadi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Green Algae ◽  
Total Lipid ◽  
Lipid Content ◽  
Unsaturated Fatty Acids ◽  
Total Lipid Content ◽  
Marine Macroalgae ◽  
Biodiesel Production ◽  
Brown Seaweeds

Abstract The aim of this work was to describe and compare the main fatty acids and biodiesel indices of some green and brown macroalgae (seaweeds) collected from the Persian Gulf, as an alternative raw material for renewable biodiesel production. The macroalgae showed low lipid content (< 10% DW) but marine macroalgae with total lipid content > 5% DW are a good source for biodiesel production. The total lipid content and saturated fatty acids (SFAs) of green algae were higher than that of brown algae, while higher accumulation of unsaturated fatty acids (USFAs) was observed in brown seaweeds. Further, the main fatty acid in all studied seaweeds was palmitic acid (C16:0), which was followed by oleic acid (C18:1). The results of this work showed that three of the green algae, especially C. sertularioides, could be a potential source of fatty acids for biodiesel production owing to their high total lipid content, high cold flow indices (long chain saturated factor, cold filter plugging point and cloud point) and a fatty acid profile rich in SFAs with a high amount of C18:1, which is suitable for oil-based bio products. In contrast, the brown seaweeds Sargassum boveanum and Sirophysalis trinodis lipid content had a high proportion of polyunsaturated fatty acids (PUFAs), which makes them suitable for replacing fish oil.

scholarly journals Influence of Light Conditions on Microalgae Growth and Content of Lipids, Carotenoids, and Fatty Acid Composition

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1060
Author(s):  
Yevhen Maltsev ◽  
Kateryna Maltseva ◽  
Maxim Kulikovskiy ◽  
Svetlana Maltseva
Keyword(s):  
Fatty Acids ◽  
Light Intensity ◽  
Saturated Fatty Acids ◽  
Spectral Composition ◽  
Value Added ◽  
Biomass Productivity ◽  
Light Conditions ◽  
Strong Light ◽  
The Impact ◽  
Microalgae Growth

Microalgae are a valuable natural resource for a variety of value-added products. The growth of microalgae is determined by the impact of many factors, but, from the point of view of the implementation of autotrophic growth, light is of primary importance. This work presents an overview of the influence of light conditions on the growth of microalgae, the content of lipids, carotenoids, and the composition of fatty acids in their biomass, taking into account parameters such as the intensity, duration of lighting, and use of rays of different spectral composition. The optimal light intensity for the growth of microalgae lies in the following range: 26−400 µmol photons m−2 s−1. An increase in light intensity leads to an activation of lipid synthesis. For maximum lipid productivity, various microalgae species and strains need lighting of different intensities: from 60 to 700 µmol photons m−2 s−1. Strong light preferentially increases the triacylglyceride content. The intensity of lighting has a regulating effect on the synthesis of fatty acids, carotenoids, including β-carotene, lutein and astaxanthin. In intense lighting conditions, saturated fatty acids usually accumulate, as well as monounsaturated ones, and the number of polyunsaturated fatty acids decreases. Red as well as blue LED lighting improves the biomass productivity of microalgae of various taxonomic groups. Changing the duration of the photoperiod, the use of pulsed light can stimulate microalgae growth, the production of lipids, and carotenoids. The simultaneous use of light and other stresses contributes to a stronger effect on the productivity of algae.

scholarly journals Effect of season on the fatty acid profile of total lipids, phospholipids and triacylglycerols in Mastacembelus mastacembelus (Atatürk Dam Lake, Turkey)

2018 ◽  
Vol 69 (1) ◽  
pp. 242 ◽  
Author(s):  
S. Kaçar ◽  
M. Başhan ◽  
S. A. Oymak
Keyword(s):  
Fatty Acids ◽  
Total Lipid ◽  
Lipid Content ◽  
Palmitoleic Acid ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
Docosapentaenoic Acid ◽  
Monounsaturated Fatty Acids ◽  
Total Lipids ◽  
Atatürk Dam Lake

The present study investigated the lipid content and FA profiles of total lipids, phospholipids (PL) and triacylglycerols (TAG) of Mastacembelus mastacembelus from the Atatürk Dam Lake (Turkey). The results showed that the total lipid content of the dorsal muscle varied seasonally from 0.50% to 3.59%. Wide ranges of saturated fatty acids (SFAs) (36.21 to 50.52%) and polyunsaturated fatty acids (PUFAs) (25.22 to 42.02%) were found in the PL fraction. However, higher monounsaturated fatty acids (MUFAs) (37.63 to 45.07%) and SFA (34.71 to 38.82%) were found in TAG (triacylglycerol). The ratios of ω-3 to ω-6 PUFA ranged from 0.65 to 1.32 and 1.07 to 3.48 in PL (phospholipid) and TAG fraction, respectively. The results also showed that the major components were palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1ω-9), palmitoleic acid (C16:1ω-7), docosahexaenoic acid (DHA, C22:6ω-3), arachidonic acid (AA, C20:4ω-6) and docosapentaenoic acid (C22:5ω-3) in total lipid, C16:0, C18:1ω-9, AA and DHA in PL, C16:0, C16:1ω-7, C18:1ω-9, linoleic acid (LA, C18:2ω-6), and DHA in TAG extracted from the muscle of M. mastacembelus in all seasons.

scholarly journals Comparative Lipid Composition Study in Farmed and Wild Blackspot Seabream (Pagellus bogaraveo)

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S274-S276 ◽  
Author(s):  
V. Álvarez ◽  
M. Trigo ◽  
S. Lois ◽  
D. Fernández ◽  
I. Medina ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Lipid Composition ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
Alpha Tocopherol ◽  
Farmed Fish ◽  
Composition Study ◽  
Pagellus Bogaraveo

During the latest decades, the fish trade has accorded an increasing attention to aquaculture development as a source of marine food products. In this sense, blackspot seabream (<I>Pagellus bogaraveo</I>) has recently attracted a great commercial interest as a farmed product. This work provides a comparative lipid composition study between wild and farmed individuals. For it, three different edible zones (ventral, dorsal and tail white muscles) were considered. From a nutritional point of view, lipid composition of farmed and wild blackspot seabream showed valuable lipid parameters (total polyunsaturated fatty acids, total ω3/total ω6 fatty acid ratio and &alpha;-tocopherol values). Comparison between farmed and wild fishes led to a wide number of differences. Thus, farmed fish muscle showed a higher (<I>P</I> < 0.05) total lipid content than its counterpart wild one. In addition, farmed fish showed higher triglyceride contents but lower (<I>P</I> < 0.05) values in the remaining lipid classes and groups studied (phospholipids, sterols and α-tocopherol). Concerning the fatty acid analysis, farmed fish showed a higher (<I>P</I> < 0.05) monounsaturated fatty acids (ventral and tail zones) content but lower for polyunsaturated fatty acids (ventral and tail zones) and total ω3/ total ω6 ratio. No differences (<I>P</I> > 0.05) were detected between both kinds of fish for the saturated fatty acids proportion. For all parameters studied, both farmed and wild fishes provided very little differences (<I>P</I> < 0.05) among the different muscle zones considered, so that an inhomogeneous distribution in the actual species could not be concluded.

scholarly journals Production of metabolites from Scenedesmus sp. and a microalgal consortium cultured in unconventional media

2021 ◽  
Vol 47 (2) ◽  
Author(s):  
Kevin A González-Falfán ◽  
Claudia Guerrero-Barajas ◽  
Jesús A Badillo-Corona ◽  
Luis C Fernández-Linares
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Tap Water ◽  
Low Cost ◽  
Saturated Fatty Acids ◽  
Biomass Productivity ◽  
Treated Wastewater ◽  
Piggery Wastewater ◽  
Nutrient Contents ◽  
The Media

Growth comparisons were made between a microalgal consortium and Scenedesmus sp. cultivated in treated wastewater (TWw) enriched with 1 mL·L–1 Bayfolan Forte fertilizer (BM), TWw enriched with (NH4)2HPO4 (PAM), TWw enriched with NH4HCO3 (BCAM), tap water with piggery wastewater (PEM), tap water with piggery wastewater digestate (PDM), and raw wastewater (Ww). Nitrogen (N) content in the media, except for TWw, was adjusted to 80 mg·L–1 N (NH4+-N and NO3–-N). Unconventional low-cost media with lower nutrient contents (BM and TWw) showed adequate productions of biomass and lipids. PEM was the most advantageous medium, showing the highest biomass productivity with the consortium (191.25 ± 6.25 g·L–1·d–1) and a lipid productivity of 36.75 ± 9.90 mg·L–1·d–1. The fatty acid profile was composed mainly of C16 and C18. PAM, PEM, and PDM showed a higher proportion of saturated fatty acids (60%–69%), whereas the composition of unsaturated fatty acids was in the range of 31% to 38%. In PEM and PDM the removal of NH4+ was 100%; however, there were NH4+ losses (as NH3) due to volatilization (46%). Unconventional media, especially Ww, are an option for growing microalgae.

scholarly journals Lipid and fatty acid profile of liver in relation to reproduction in the small-scaled terapon Terapon puta (Cuvier, 1829) from Pondicherry Coast, India

2018 ◽  
Vol 65 ◽  
Author(s):  
R. Nandikeswari ◽  
M. Sambasivam
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Polyunsaturated Fatty Acid ◽  
Total Lipid ◽  
Lipid Content ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
Dry Weight ◽  
Monounsaturated Fatty Acids

<p>The total lipid content as percentage of dry weight of liver of <em>Terapon puta</em> (Cuvier, 1829) varied at four different stages <em>viz.,</em> immature, maturing, mature and spent stages of reproduction. Highest levels of saturated fatty acids (SFA) was recorded in the liver of mature fishes (50.71%) followed by maturing (48.03%) and the lowest in immature fishes (38.91%). Highest levels of monounsaturated fatty acids (MUFA) were observed in the liver of maturing fishes (37.07%) followed by mature (34.09%) and the lowest levels in immature fishes (30.48%). Maturing fishes had higher liver polyunsaturated fatty acid  levels (PUFA) (13.54%), compared to immature (10.55%) and mature fishes (10.17%).</p>

Dietary Changes in Fasting Levels of Factor VII Coagulant Activity (FVII: C) Are Accompanied by Changes in Factor VII Protein and other Vitamin K-dependent Proteins

1995 ◽  
Vol 73 (02) ◽  
pp. 239-242 ◽  
Author(s):  
E M Bladbjerg ◽  
T Tholstrup ◽  
P Marckmann ◽  
B Sandström ◽  
J Jespersen
Keyword(s):  
Fatty Acids ◽  
Vitamin K ◽  
Protein C ◽  
Saturated Fatty Acids ◽  
Factor Vii ◽  
Dietary Regimen ◽  
Blood Samples ◽  
Dietary Changes ◽  
Coagulant Activity ◽  
Before And After

SummaryThe mechanisms behind dietary effects on fasting coagulant activity of factor VII (FVII: C) are not clarified. In the present study of 15 young volunteers, two experimental diets differing in composition of saturated fatty acids (C18:0 [diet S] or C12:0 + C14:0 [diet ML]) were served for 3 weeks each. Fasting blood samples were collected before and after the dietary regimen and analysed for triglycerides, FVII:C, and protein concentrations of FVII, FII, FX, protein C, CRP, albumin, fibrinogen, and F1+2. FVII:C was significantly reduced on diet S compared with diet ML. This was accompanied by a decrease in FVII protein, F1+2 and the vitamin K-dependent proteins FII, FX, and protein C. In contrast, no changes were observed in triglycerides, FVII:C/FVII: Ag, albumin and CRP. Fibrinogen was increased on diet S compared with diet ML. Our findings suggest that the change in fasting FVII:C was part of a general change in concentrations of vitamin K-dependent proteins.

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