scholarly journals Bacillus subtilis DSM29784 Alleviates Negative Effects on Growth Performance in Broilers by Improving the Intestinal Health Under Necrotic Enteritis Challenge

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuanyuan Wang ◽  
Yibin Xu ◽  
Shengliang Xu ◽  
Jinyong Yang ◽  
Kaiying Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Performance ◽  
Economic Losses ◽  
Broiler Chicks ◽  
Necrotic Enteritis ◽  
Negative Effects ◽  
Treatment Groups ◽  
Beneficial Effects ◽  
Intestinal Lesions ◽  
Colony Forming Units

Along with banning antibiotics, necrotic enteritis (NE), especially subclinical enteritis (SNE), poses a significant threat to the chicken industry; however, probiotics are a potentially promising intervention. We aimed to investigate the beneficial effects of Bacillus subtilis DSM29784 (BS) on the treatment of Clostridium perfringens (CP)-induced SNE in broilers. A total of 360 1-day-old broiler chicks were divided into three treatment groups, namely control (Ctr), SNE, and BS treatment (BST) groups, all of which were fed with a basal died for 21days, and then from day 22 onward, only the BST group had a BS supplemented diet (1×109 colony-forming units BS/kg). On day 15, all chicks, except the Ctr group, were challenged with a 20-fold dose coccidiosis vaccine and 1ml CP (2×108) on days 18–21 for SNE induction. Beneficial effects were observed on growth performance in BST compared to SNE broilers. BST treatment alleviated intestinal lesions and increased the villus height/crypt depth ratio. Further, BST broilers showed increased maltase activity in the duodenum compared with SNE chicks, and a significantly decreased caspase-3 protein expression in the jejunum mucosa. Moreover, an increased abundance of Ruminococcaceae and Bifidobacterium beneficial gut bacteria and an altered gut metabolome were observed. Taken together, we demonstrate that the manipulation of microbial gut composition using probiotics may be a promising prevention strategy for SNE by improving the composition and metabolism of the intestinal microbiota, intestinal structure, and reducing inflammation and apoptosis. Hence, BS potentially has active ingredients that may be used as antibiotic substitutes and effectively reduces the economic losses caused by SNE. The findings of this study provide a scientific foundation for BS application in broiler feed in the future.

scholarly journals Supplemental Bacillus subtilis PB6 Improves Growth Performance and Gut Health in Broilers Challenged with Clostridium perfringens

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yan Liu ◽  
Song Zhang ◽  
Zheng Luo ◽  
Dan Liu
Keyword(s):  
Bacillus Subtilis ◽  
Growth Performance ◽  
Clostridium Perfringens ◽  
Basal Diet ◽  
Economic Losses ◽  
Control Group ◽  
Necrotic Enteritis ◽  
Dosage Group ◽  
Microbial Composition ◽  
High Dosage Group

Clostridium perfringens (CP) is the principal pathogenic bacterium of chicken necrotic enteritis (NE), which causes substantial economic losses in poultry worldwide. Although probiotics are known to provide multiple benefits, little is known about the potential effects of Bacillus subtilis (B. subtilis) application in preventing CP-induced necrotic enteritis. In this study, 450 male Arbor Acres broilers were divided into 5 experimental treatments: A: basal diet (control group); B: basal diet and CP challenge (model group); C: CP challenge+10 mg/kg enramycin (positive control group); D: CP challenge+ 4 × 10 7   CFU / kg of feed B. subtilis PB6 (PB6 low-dosage group); and E: CP challenge+ 6 × 10 7   CFU / kg of feed B. subtilis PB6 (PB6 high-dosage group). There were 6 replicate pens per treatment with 15 broilers per pen. The present research examined the effect of Bacillus subtilis PB6 (B. subtilis PB6) on growth performance, mRNA expression of intestinal cytokines and tight junctions, and gut flora composition in broilers challenged with CP. The entire experiment was divided into two phases: the non-CP challenge phase (d0–18) and the CP challenge phase (d18–26). PB6 did not increase the growth performance during the first stage, but the PB6 high-dosage group was found to have larger body weight gain and ADFI during the CP challenge stage. Feed supplementation with PB6 reduced the lesion score of challenged chicks, with increased tight junction-related gene expression (occludin and ZO-1) and decreased TNF-α expression compared with CP-infected birds. A decrease in the abundance of Clostridium XI, Streptococcus, and Staphylococcus was observed after CP infection ( P < 0.05 ), while supplementation with PB6 restored the ileal microbial composition. In conclusion, administration of B. subtilis PB6 improved growth performance, enhanced intestinal barrier function, and mitigated intestinal inflammation/lesions, which might be due to its restoring effects on the ileal microbial composition in CP-challenged broilers.

scholarly journals Supplementing Garlic Nanohydrogel Optimized Growth, Gastrointestinal Integrity and Economics and Ameliorated Necrotic Enteritis in Broiler Chickens Using a Clostridium perfringens Challenge Model

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2027
Author(s):  
Doaa Ibrahim ◽  
Tamer Ahmed Ismail ◽  
Eman Khalifa ◽  
Shaimaa A. Abd El-Kader ◽  
Dalia Ibrahim Mohamed ◽  
...  
Keyword(s):  
Growth Performance ◽  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Economic Benefits ◽  
Production Costs ◽  
Broiler Chicks ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Intestinal Lesion

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.

scholarly journals Effects of cLFchimera peptide on intestinal morphology, integrity, microbiota, and immune cells in broiler chickens challenged with necrotic enteritis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ali Daneshmand ◽  
Hassan Kermanshahi ◽  
Mohammad Hadi Sekhavati ◽  
Ali Javadmanesh ◽  
Monireh Ahmadian ◽  
...  
Keyword(s):  
Immune Cells ◽  
Synthetic Peptide ◽  
Broiler Chickens ◽  
Intestinal Morphology ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Necrotic Enteritis ◽  
Gut Health ◽  
Junctional Proteins ◽  
Intestinal Lesions

Abstract Three hundred and sixty 1-day-old male broiler chicks were randomly allocated to 4 treatments of 6 replicates to evaluate the effects of cLFchimera, a recombinant antimicrobial peptide (AMP), on gut health attributes of broiler chickens under necrotic enteritis (NE) challenge. Treatments were as follows: (T1) unchallenged group fed with corn-soybean meal (CSM) without NE challenge and additives (NC); (T2) group fed with CSM and challenged with NE without any additives (PC); (T3) PC group supplemented with 20 mg cLFchimera/kg diet (AMP); (T4) PC group supplemented with 45 mg antibiotic (bacitracin methylene disalicylate)/kg diet (antibiotic). Birds were sampled for villi morphology, ileal microbiota, and jejunal gene expression of cytokines, tight junctions proteins, and mucin. Results showed that AMP ameliorated NE-related intestinal lesions, reduced mortality, and rehabilitated jejunal villi morphology in NE challenged birds. While the antibiotic non-selectively reduced the count of bacteria, AMP restored microflora balance in the ileum of challenged birds. cLFchimera regulated the expression of cytokines, junctional proteins, and mucin transcripts in the jejunum of NE challenged birds. In conclusion, cLFchimera can be a reliable candidate to substitute growth promoter antibiotics, while more research is required to unveil the exact mode of action of this synthetic peptide.

scholarly journals Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens

2019 ◽  
Vol 7 (3) ◽  
pp. 71 ◽  
Author(s):  
Cristiano Bortoluzzi ◽  
Bruno Serpa Vieira ◽  
Juliano Cesar de Paula Dorigam ◽  
Anita Menconi ◽  
Adebayo Sokale ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Clostridium Perfringens ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Dietary Supplementation ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Negative Effects ◽  
Infected Control

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 106 CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18–20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens.

scholarly journals Effect of dried Bacillus subtilis culture on growth, body composition and hepatic lipogenic enzyme activity in female broiler chicks

1995 ◽  
Vol 74 (4) ◽  
pp. 523-529 ◽  
Author(s):  
U. Santoso ◽  
K. Tanaka ◽  
S. Ohtani
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Feed Efficiency ◽  
Abdominal Fat ◽  
Control Group ◽  
Broiler Chicks ◽  
Lipogenic Enzyme ◽  
Treatment Groups

To investigate the effect of dried Bacillus subtilis culture on growth, body composition and hepatic lipogenic enzyme activity, female broiler chicks were fed on either no additive (control) or dried B. subtills-culture-supplemented commercial diets (215 g crude protein/kg, 12.85 MJ metabolizable energy/kg) at 10 or 20 g/kg diet for 28 d from 14 to 42 d of age. Body weight, and moisture, fat, protein and ash contents of the body were not influenced by the B. subtilis culture. Feed efficiency. N utilization, the ratio of abdominal fat or liver to body weight, acetyl-coenzyme A carboxylase (EC 6·4·1·2) activity, liver and serum cholesterol contents were significantly lower in treatment groups, while fatty acid synthetase activity and serum cholesterol concentration were not significantly different, compared with the control group. Liver triacylglycerol concentration was decreased in chicks given 20 g culture·kg diet, while serum and carcass triacylglycerol concentrations were significantly lower in treatment groups than in the control group. Serum phospholipid concentration was increased but carcass phospholipid concentration was decreased in chicks given 20 g B. subtilis/kg diet, while liver phospholipid concentration was not significantly influenced. The advantages of inclusion of B. subtilis to the broiler diet included improved feed efficiency, less abdominal fat, reduced triacylglycerol concentrations in the liver, serum and carcass and reduced cholesterol concentrations in the liver and carcass.

scholarly journals Probiotic feeding effect of Bacillus subtilis on broilers chicks’ microflora, TLRs and interleukin gene expression

2021 ◽  
Author(s):  
Zaigui Wang ◽  
Ru Yang ◽  
Salman Khan ◽  
Chunjie wei ◽  
Hu Qian ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Protease Activity ◽  
Broiler Chicken ◽  
Amylase Activity ◽  
Digestive Enzyme ◽  
Digestive Enzyme Activity ◽  
Control Group ◽  
Broiler Chicks ◽  
Treatment Groups

Abstract The broiler chicks provide the major portion of daily food items. The current study aimed to investigate the effects of Bacillus subtilis (BS) on broiler chicken gut microflora diversity, digestive enzyme activity and expression of Toll-like receptors (TLRs). A total of 240 crossbred broiler chickens were randomly allocated into 4 groups with 3 replicates. The groups were named as control group (basal diet, BD), group Ⅰ (BD with 300 g/d BS,1.08×107 CFU/kg), group Ⅱ (BD with 600 g/d BS, 2.16×107 CFU/kg), and group Ⅲ (BD with 900 g/d BS, 3.24×107 CFU/kg). Samples were collected at the 21st day. The difference of α diversity and β diversity between control group and treatment groups were not significant, but the abundance of some microorganisms in the treatment groups were improved, and the core microorganisms were different between groups. In comparison of control group, the protease activity in ileum of experimental groups was significantly increased (p < 0.05). Protease activity in ileum of group Ⅱ and Ⅲ was improved by 22.59% (p < 0.01) and 14.49% (p < 0.05). The amylase activity in ileum of treatment groups were also significantly increased (p < 0.05). Amylase activity of group Ⅱ was increased by 41.85% (p < 0.01) in comparison to control, group Ⅰ and Ⅲ. Expression of TLR1A and TLR7 in the jejunum and caecum of treatment groups were significantly up-regulated while their expression in ileum was decreased. Similarly, the expression of TLR1B gene was significantly improved in the ileum, and down regulated in the cecum. Transcription levels of TLR2A and MyD88 in jejunum, liver, spleen and kidney were significantly increased, but their expression was significantly lower in the ileum and cecum. The TLR2B, TLR3, TLR4, TLR15, TLR21 expression were also significantly modulated in various organ of broiler chicken. The most important inflammatory factor such as IL-1β expressions in spleen and kidney were significantly increased in the current data. Bacillus subtilis could not regulate the caecal microflora diversity, but improved amount of some gut probiotics such as lactobacillus, the digestive enzyme activity, regulate some immunogenic expressions and enhance the immune capacity of animal. We hope that the impact of this data will explore the diseases control and feeding quality of broiler chicks at industrial level.

scholarly journals Effects of Turmeric Powder on Clostridium Perfringens Load in Broiler Chickens

2020 ◽  
Vol 18 (1) ◽  
pp. 209-218
Author(s):  
MZ Ali ◽  
MM Islam ◽  
S Zaman
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Economic Problem ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Control Groups ◽  
Treatment Groups ◽  
Environment Control

Necrotic enteritis (NE) is a major economic problem in broiler industry globally and is caused by Clostridium perfringens. The aim of the study was to know the effects of turmeric on C. perfringens in broiler chickens. A total of 3000-day-old Cobb 500 broiler chicks were divided into 6 groups and reared in environment control sheds with similar management. Each group contains 500 chicks and again divided into two subgroups as control and treatment with 250 chicks. In treatment groups added 2gm/kg turmeric powder with basal feeds and reared up to 30 days and follow standard vaccine schedule. Intestinal samples were collected every week from each group to detect C. perfringens load. Bodyweight gain, feed conversion ratio (FCR) and mortality rates were also calculated. The findings were loads of C. perfringens in treatment groups decreased significantly compared to control groups. At 4th week average count of C. perfringens was 4.44±0.12 log CFU/g and 2.68±0.17 log CFU/g in control and treatment groups, respectively. Average chick’s mortality decreased significantly in treatment groups. The flock mortality was decreased significantly in treatment groups (1.40%) compared to control groups (2.17%). The FCR become significantly decreased in treatment groups (1.490) compared to control groups (1.571). Therefore, use of turmeric powder in broiler ration can reduce NE by decreasing C. perfringens loads and it could be a good source of non-antibiotic growth promoter in poultry towards reduce antibiotic resistance and consumer will get a pathogen free rich protein source. SAARC J. Agri., 18(1): 209-218 (2020)

scholarly journals Using different levels of glycerine, glucose, or sucrose in broiler starter diets to overcome negative effects of delayed feed access on growth performance

2018 ◽  
Vol 98 (2) ◽  
pp. 311-324
Author(s):  
Anhao Wang ◽  
Derek Anderson ◽  
Bruce Rathgeber
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Conversion Ratio ◽  
Crude Glycerine ◽  
Feed Conversion Ratio ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Negative Effects ◽  
Feeding Programs ◽  
Different Levels

Two trials were conducted to evaluate the inclusion of glycerine, glucose, or sucrose in broiler starter diets to overcome negative effects of delayed feed access. In trial 1, 2160 newly hatched chicks (mixed sexed) were randomly assigned to two feeding programs (immediate vs. 36 h delayed feed access). Nine starter diets prepared with crude glycerine, glucose, or sucrose fed at 0%, 4%, or 8% levels were fed during the first 14 d post hatch (PH). In trial 2, 720 male and 720 female newly hatched chicks were randomly assigned to two feeding programs (immediate vs. 48 h delayed feed access). Starter diets fed during the first 14 d of feed access, consisted of a control, 8% glycerine, or 8% glucose. In both trials, a common grower and finisher diet were fed during days 14–24 and 25–35, respectively. Body weight and feed consumed were measured on 7, 14, 24, and 35 d. In trial 1, all 8% diet-fed birds were heavier compared with the control on day 35 PH (2259 vs. 2198 g, P < 0.01). Birds fed glycerine starter diets had improved feed conversion ratio (FCR) during 22–35 d (P < 0.01). In trial 2, glycerine diet resulted in heavier birds after 35 d of feed access, compared with birds fed control and glucose diets immediately after hatch (2373 vs. 2276 g, P < 0.01). Dietary inclusion of 8% glycerine can assist newly hatched broiler chicks to overcome delayed feed access induced growth suppression.

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