scholarly journals Comparative Effects of L. plantarum CGMCC 1258 and L. reuteri LR1 on Growth Performance, Antioxidant Function, and Intestinal Immunity in Weaned Pigs

2021 ◽  
Vol 8 ◽  
Author(s):  
Qingsong Tang ◽  
Hongbo Yi ◽  
Weibin Hong ◽  
Qiwen Wu ◽  
Xuefen Yang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Mucosa ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Intestinal Immunity ◽  
Weaned Pigs ◽  
Tnf Α ◽  
Antioxidant Function ◽  
Ifn Γ ◽  
Diarrheal Incidence

Lactobacillus plantarum CGMCC 1258 and Lactobacillus reuteri LR1 are two important strains of probiotics. However, their different advantages in the probiotic effect of weaned pigs are still poorly understood. Therefore, the study was to investigate the comparative effects of dietary supplementation of L. plantarum CGMCC 1258 and L. reuteri LR1 on growth performance, antioxidant function, and intestinal immunity in weaned pigs. Ninety barrows [initial body weight (BW) = 6.10 ± 0.1 kg] 21 days old were randomly divided into 3 treatments with 5 replicates, each replicate containing 6 pigs. Pigs in control (CON) were fed a basal diet, and the basal diets supplemented with 5 × 1010 CFU/kg L. plantarum CGMCC 1258 (LP) or L. reuteri LR1 (LR) for 42 days, respectively. The results showed that LP increased (p < 0.05) serum superoxide dismutase (SOD), and decreased (p < 0.05) serum malondialdehyde (MDA) and the expression and secretion of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in intestinal mucosa, but has no significant effect on growth performance and diarrheal incidence. However, LR increased (p < 0.05) final BW and average daily gain (ADG), reduced (p < 0.05) 29–42-day diarrheal incidence, decreased (p < 0.05) the expression and secretion of IL-1β, IL-6, TNF-α, and IFN-γ, and increased (p < 0.05) the expression of transforming growth factor-β (TGF-β) in intestinal mucosa. In addition, the serum glutathione peroxidase (GSH-PX), mRNA relative expression of Na+-K+-2Cl– co-transporter 1 (NKCC1) and cystic fibrosis transmembrane conductance regulator (CFTR) and the content of toll-like relative (TLR2) and TLR4 in the jejunum, and secretory immunoglobulin (sIgA) content of ileal mucosa were higher (p < 0.05) than LP. Collectively, dietary L. plantarum CGMCC 1258 improved intestinal morphology, intestinal permeability, intestinal immunity, and antioxidant function in weaned pigs. Dietary L. reuteri LR1 showed better growth performance, a lower incidence of diarrhea, better intestinal morphology, and a higher extent of immune activation in weaned pigs.

scholarly journals Effects of dietary seaweed extract supplementation in sows and post-weaned pigs on performance, intestinal morphology, intestinal microflora and immune status

2011 ◽  
Vol 106 (5) ◽  
pp. 688-699 ◽  
Author(s):  
S. G. Leonard ◽  
T. Sweeney ◽  
B. Bahar ◽  
B. P. Lynch ◽  
J. V. O'Doherty
Keyword(s):  
Growth Performance ◽  
Intestinal Microflora ◽  
Immune Status ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Seaweed Extract ◽  
Gut Health ◽  
Weaned Pigs ◽  
Treatment Groups ◽  
Daily Gain

The present study investigated the effects of dietary supplementation of a seaweed extract (SWE) to sows and weaned pigs on post-weaning growth performance, intestinal morphology, intestinal microflora, volatile fatty acid concentrations and immune status of pigs at days 11 and 117 post-weaning. Gestating sows (n20) were supplemented with a SWE (0v.10·0 g/d) from day 107 of gestation until weaning (day 26). At weaning, pigs (four pigs per sow) were divided into two groups based on sow diet during lactation and supplemented with a SWE (0v.2·8 g/kg diet), resulting in four treatment groups: (1) BB (basal sows–basal pigs); (2) BS (basal sows–treated pigs); (3) SB (treated sows–basal pigs); (4) SS (treated sows–treated pigs). Pigs weaned from SWE-supplemented sows had a higher average daily gain (ADG) between days 0 and 21 (P < 0·05) post-weaning compared with pigs weaned from non-SWE-supplemented sows. Pigs offered post-weaning diets (PW) containing SWE had decreased colonicEscherichia colipopulations on day 11 (P < 0·01) and decreased colonic Enterobacteriaceae numbers on day 117 (P < 0·05). Pigs offered PW containing SWE had a greater mRNA abundance ofMUC2in the colon at day 11 post-weaning (P < 0·05) compared with pigs offered unsupplemented diets. In conclusion, these results demonstrate that SWE supplementation post-weaning provides a dietary means to improve gut health and to enhance growth performance in starter pigs. Dietary SWE supplementation increased ADG during the grower–finisher (GF) phases. However, there was no growth response to SWE inclusion in GF diets when pigs were weaned from SWE-supplemented sows.

scholarly journals Effects of Zinc Oxide and Arginine on the Intestinal Microbiota and Immune Status of Weaned Pigs Subjected to High Ambient Temperature

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1537
Author(s):  
Se Young Yoon ◽  
Soo Jin Sa ◽  
Eun Seok Cho ◽  
Han Seo Ko ◽  
Jung Woo Choi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Performance ◽  
Mrna Expression ◽  
Intestinal Microbiota ◽  
Immune Status ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Weaned Pigs ◽  
Tnf Α ◽  
Clostridium Spp

This study aimed to investigate the effect of the l-arginine (Arg) inclusion and different doses of ZnO on the growth performance, intestinal microbiota and integrity, and immune status of weaned pigs. A total of 180 pigs (28-day-old) were randomly allotted to six treatments with six replicate pens in each treatment and five pigs per pen. The dietary treatments were Con (1.1% Arg); P-Zn (1.1% Arg + 2500 mg Zn as ZnO/kg diet); ARG (1.6% Arg); ZnArg1 (500 mg of Zn as ZnO/kg diet + 1.6% Arg); ZnArg2 (1000 mg of Zn as ZnO/kg diet + 1.6% Arg); ZnArg3 (2500 mg of Zn as ZnO/kg diet + 1.6% Arg). The overall result showed that the inclusion of ZnArg3 significantly improved the average daily gain of pigs compared with the Con treatment. There was a reduction in feed intake in pigs fed the Con diet compared with pigs fed the ZnArg3 diet at phase 1 and overall. At phase 1, pigs fed the ZnArg3 diet and P-Zn diet showed a decreased population of Clostridium spp. in the ileum compared with those of the Con treatment. In addition, a lower ileal Clostridium spp. population was detected in pigs fed the ZnArg2 diet compared with pigs fed the Con diet. The pigs fed ZnArg1 and ZnArg3 diets showed a greater villus height of duodenum compared with the Con and P-Zn treatments. The pigs in the Con treatment showed increased mRNA expression of heat shock protein-27 in the liver compared with the P-Zn, ZnArg1, ZnArg2, and ZnArg3 treatments. When fed the basal diet, mRNA expressions of interleukin-6 were increased in the muscle compared with the ZnArg3 treatment. Dietary supplementation with ZnArg2 decreased the mRNA expressions of interferon-γ in the muscle compared with the Con treatment. Supplementation with P-Zn, ZnArg1, ZnArg2, and ZnArg3 decreased mRNA expressions of tumor necrosis factor-α (TNF-α) compared with the Con treatment. The mRNA gene expressions of interleukin-4 were decreased in the jejunum of pigs fed P-Zn, ARG, ZnArg1, ZnArg2, and ZnArg3 diets compared with pigs fed the Con diet. The jejunum gene expression of toll-like receptor-4 was upregulated in the Con and ARG treatments compared with the ZnArg1 and ZnArg3. The ZnArg1, ZnArg2, and ZnArg3 treatments showed lower mRNA expression of TNF-α compared with the Con treatment. In conclusion, there was no difference in growth performance, intestinal microbiota, gene expression of interleukins between ZnArg1 and ZnArg3 treatments. Therefore, the low level of ZnO (500 mg/kg) plus 1.6% dietary Arg may be recommended for pigs during the weaning stress.

scholarly journals Dietary ZnO and arginine supplementation on the dynamic change of microbiota, intestinal morphology, and immune function of weaned pigs subjected to heat stress

2020 ◽  
Author(s):  
Se Young Yoon ◽  
SooJin Sa ◽  
Eun Seok Cho ◽  
HanSeo Ko ◽  
Ga Won Kim ◽  
...  
Keyword(s):  
Heat Stress ◽  
Growth Performance ◽  
Mrna Expression ◽  
Immune Status ◽  
Phase 1 ◽  
Dietary Supplementation ◽  
Intestinal Morphology ◽  
Weaned Pigs ◽  
Weaning Stress ◽  
Tnf Α

Abstract Background. Weaning stress is an economically important problem in the pigs, and the economic loss of the growth performance reduction is even more critical if the heat stress adds to the weaning stress. The supplementation of ZnO is an effective option in reducing the adverse effects of weaning time. This study aimed to investigate the effect of the L-arginine (Arg) inclusion and different doses of ZnO to determine the best dietary supplementation ratio on growth performance, intestinal microbiota and integrity, and immune status in weaned pigs. A total of 180 weaned pigs (28 day-old) were randomly allotted to six treatments with 6 replicate pens in each treatment and 5 pigs per pen. The dietary treatments were: control diet (Con; with 1.1% Arg and without ZnO supplementation); Con + 2500 ppm Zn as ZnO (P-Zn); Con + 1.6% Arg (ARG); Con + 500 ppm of Zn as ZnO + 1.6% Arg (ZnArg1); Con + 1000 ppm of Zn as ZnO + 1.6% Arg (ZnArg2); P-Zn + 1.6% Arg (ZnArg3). Results. The overall result showed that the inclusion of ZnArg3 significantly improved the average daily gain compared with the Con treatment. There was a reduction of feed intake in the Con diet compared with the ZnArg3 diet at phase 1 and overall. At phase 1, the weaned pigs in the ZnArg3 and P-Zn groups exhibited the decreased population of Clostridium spp. in the ileum compared with those of the Con group. In addition, a lower ileal Clostridium spp. population was detected in the ZnArg2 pigs compared with the Con pigs. At phase 2, the colonization of Clostridium spp. was higher in the Con and ARG treatments compared with ZnArg3 treatment. The weaned pigs fed the ZnArg1 and ZnArg3 diets showed a greater villus height of duodenum compared with the Con and P-Zn treatments. The count of eosinophil was significantly higher in the Con and ZnArg1 compared with the ZnArg2 and ZnArg3 treatments. The weaned pigs in the Con group showed increased mRNA expression of HSP27 in the liver compared with the P-Zn, ZnArg1, ZnArg2, and ZnArg3 groups. When fed the basal diet, weaned pigs exhibited enhanced mRNA expressions of IL-6 in the muscle compared with the ZnArg3 group. Dietary supplementation with ZnArg2 decreased the mRNA expressions of IFNγ in the muscle compared with the Con group. Supplementation with P-Zn, ZnArg1, ZnArg2, and ZnArg3 exhibited decreased mRNA expressions of TNF-α compared with the Con group. The mRNA gene expressions of IL-4 were decreased in the jejunum of P-Zn, ARG, ZnArg1, ZnArg2, and ZnArg3 weaned pigs compared with the Con group. The jejunum gene expression of TLR4 was upregulated in the Con and ARG treatments compared with the ZnArg1 and ZnArg3. The ZnArg1, ZnArg2, and ZnArg3 treatments showed a lower mRNA expression of TNF-α compared with the Con group. Conclusion. The Arg supplementation did not improve the growth performance, microbial composition, or immune status of weaned pigs but showed a similar growth performance when supplemented with 500 ppm Zn as ZnO compared with 2500 ppm Zn as ZnO.

scholarly journals Dietary Glutamic Acid Modulates Immune Responses and Gut Health of Weaned Pigs

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 504
Author(s):  
Hyunjin Kyoung ◽  
Jeong Jae Lee ◽  
Jin Ho Cho ◽  
Jeehwan Choe ◽  
Joowon Kang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Immune Responses ◽  
Glutamic Acid ◽  
Growth Performance ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Intestinal Morphology ◽  
Weaned Pigs ◽  
Tnf Α

Dietary glutamic acid (GLU) is used as a feed additive because of its functional characteristics that may affect the growth performance and health of pigs. This study was carried out to determine the effects of dietary GLU on growth performance, nutrient digestibility, immune responses, and intestinal health of weaned pigs. A total of ninety-six weaned pigs (8.07 ± 1.17 kg of body weight; 28 days of age) were assigned to two dietary treatments (8 pigs/pen; 6 replicates/treatment) in a randomized complete block design (block: body weight): (1) a typical weaner diet (CON) and (2) CON supplemented with 0.5% GLU. The experimental period was for 4 weeks. All data and sample collections were performed at the specific time points during the experimental period. Pigs fed GLU had higher average daily gain and average daily feed intake for the first two weeks and nutrient digestibility than pigs fed CON. In addition, dietary GLU increased villus height to crypt depth ratio, number of goblet cells, and ileal gene expression of claudin family and occludin compared with CON, but decreased serum TNF-α and IL-6 and ileal gene expression of TNF-α. Moreover, pigs fed GLU had increased relative composition of bacterial communities of genus Prevotella and Anaerovibrio and decreased genus Clostridium and Terrisporobacter compared with those fed CON. This study suggests that dietary GLU influences growth performance and health of weaned pigs by modulating nutrient digestibility, intestinal morphology, ileal gene expression of tight junction proteins and cytokines, immune responses, and microbial community in the gut.

PSIII-16 Growth Performance of Weaned Pigs Fed Barley Differing in Fermentable Starch and Fiber Profile

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 175-176
Author(s):  
Joaquin J Sanchez Zannatta ◽  
L F Wang ◽  
Eduardo Beltranena ◽  
Aaron D Beattie ◽  
Rex N Newkirk ◽  
...  
Keyword(s):  
Energy Source ◽  
Growth Performance ◽  
Steam Explosion ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Barley Grain ◽  
Gut Health ◽  
Weaned Pigs ◽  
Cereal Grain ◽  
Hulled Barley

Abstract Barley grain containing more fermentable starch or fiber might be an attractive energy source in weaned pig diets due to benefits on gut health. Barley rapidly-fermentable carbohydrates may serve as prebiotic and slowly-fermentable fiber may decrease diarrhea in weaned pigs. Steam-explosion processing may disrupt the fiber matrix of hulls, increasing slowly-fermentable fiber of barley. To explore, 220 pigs were fed 1 of 5 diets containing 60% cereal grain: 1) low-fermentable hulled barley (LFB); 2) LFB steam-exploded (LFB-E; 1.2 MPa, 120 s); 3) high β-glucan (10% DM) hull-less barley (HFB); 4) high amylose (17% DM) hull-less barley (HFA); or 5) low-fermentable wheat (LFW). Diets were fed starting 1-week post-weaning and formulated to provide 2.4 and 2.3 Mcal net energy (NE)/kg, 5.5 and 5.1 g standardized ileal digestible lysine/Mcal NE for phase 1 (day 1–14) and phase 2 (day 15–35), respectively. For the entire trial (day 1–35), average daily feed intake (ADFI) and average daily gain (ADG) of pigs did not differ among diets. Gain:feed (G:F) did not differ between LFB and LFW diets, but steam-explosion of hulled barley reduced (P &lt; 0.05) G:F. Feces consistency did not differ between LFB and LFW diets, but was better (P &lt; 0.05) for LFB than HFB, HFA and LFB-E diets. For phase 1, G:F of pigs was lower (P &lt; 0.05) for LFB-E diet than LFW diet. For days 22–28, LFB-E diet had greater (P &lt; 0.01) ADFI than HFA diet and tended (P = 0.09) to have a greater ADG than HFB diet. In conclusion, hulled or hull-less barley grain replaced wheat grain without affecting growth performance in weaned pigs. Hulled barley increased feces consistency. Steam-explosion of hulled barley did not increase growth performance of weaned pigs. Barley grain is an attractive energy source for weaned pigs for managing growth and feces consistency.

scholarly journals Effects of Dietary Taurine Supplementation to Gilts during Late Gestation and Lactation on Offspring Growth and Oxidative Stress

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 220 ◽  
Author(s):  
Mengmeng Xu ◽  
Long Che ◽  
Kaiguo Gao ◽  
Li Wang ◽  
Xuefen Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Expression ◽  
Growth Performance ◽  
Barrier Function ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Late Gestation ◽  
Villus Height ◽  
And Oxidative Stress

Birth is one of the most important events of animal production agriculture, as newborns are abruptly forced to adapt to environmental and nutritional disruptions that can lead to oxidative damage and delay in growth. Taurine (Tau) is an important regulator of oxidative stress and possesses growth-enhancing properties. In the present study, we investigated the effects of dietary Tau supplementation in gilts during late gestation and lactation on the growth performance of piglets by assessing intestinal morphology and barrier function, and oxidative stress status. Sixteen gilts were randomly allocated to the Con (basal diet) and Tau (basal diet with 1% Tau) groups from 75 d of gestation to weaning. Maternal dietary Tau supplementation significantly increased weaning weight and average daily gain weight in piglets. Piglets in the Tau group had higher villus height and villus height-to-crypt depth ratio (VCR), ZO-1 protein expression, and secretory immunoglobulin A (sIgA) content in the jejunum. Meanwhile, Tau bebeficial affected the milk quality of gilts, as indicated by decreased malondialdehyde (MDA) concentration and increased total superoxide dismutase (T-SOD), total antioxidative capability (T-AOC), glutathione peroxidase (GPx), and catalase (CAT) activity. Furthermore, Tau supplementation increased T-SOD activity in plasma and SOD2 protein expression in the jejunum in the piglets. In conclusion, this study provides evidence that dietary Tau supplementation to gilts improves growth performance in piglets, owing to improved intestinal morphology and barrier function, as well as inhibition of oxidative stress.

scholarly journals 366 Efficacy of mycotoxin deactivators on health and growth of newly weaned pigs under chronic dietary challenges of deoxynivalenol

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 130-132
Author(s):  
D M Holanda ◽  
S W Kim
Keyword(s):  
Cell Wall ◽  
Liver Function ◽  
Growth Performance ◽  
Intestinal Mucosa ◽  
Plant Extracts ◽  
Jejunal Mucosa ◽  
Yeast Culture ◽  
Intestinal Health ◽  
Weaned Pigs ◽  
Lower Serum

Abstract The efficacy of mycotoxin deactivators on health and growth performance of newly weaned pigs (27 d-old) fed diets naturally contaminated with deoxynivalenol was investigated. Sixty pigs were housed individually and assigned to 5 treatments for 34 d subdivided into 3 phases: NC (no added deoxynivalenol); PC (deoxynivalenol at 2 mg/kg); CYC (PC + clay/yeast culture based product, 0.2%); CYE (PC + clay/yeast cell wall/plant extracts/antioxidants based product, 0.2%); and CYB (PC + clay/inactivated yeast/botanicals/antioxidants based product, 0.2%). Blood was taken at d 14 and 34. Intestinal mucosa was taken at d 34. Data were analyzed using Proc Mixed of SAS with pre-planned contrasts. Deoxynivalenol reduced (P &lt; 0.05) ADG in P3. Pigs fed CYC had greater (P &lt; 0.05) ADG during overall period, ADFI during P3, and gain/feed during P2 than PC. At d 14, deoxynivalenol reduced (P &lt; 0.05) BUN/creatinine and tended to reduce (P = 0.088) BUN. Pigs fed CYB tended to have greater (P = 0.059) AST than PC. At d 34, pigs fed CYC (P = 0.083) and CYB (P = 0.068) tended to have lower serum CPK than PC. Pigs fed CYE had lower (P &lt; 0.05) BUN/creatinine than PC. Deoxynivalenol tended to increase (P = 0.068) malondialdehydes and decrease (P = 0.072) glutathione in jejunal mucosa. Pigs fed CYE and CYB had lower (P &lt; 0.05) malondialdehydes, whereas pigs fed CYB had greater (P &lt; 0.05) glutathione and tended to have lower (P = 0.079) jejunal IgA than PC. Pigs fed CYC (P = 0.066) and CYE (P = 0.099) tended to have lower jejunal IL8 than PC. In conclusion, deoxynivalenol compromised growth performance and intestinal health. The mycotoxin deactivators could enhance intestinal health of pigs fed diets with deoxynivalenol without affecting liver function.

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