scholarly journals The Content of Biologically Active Substances in Crocosmia × crocosmiiflora ‘Lucifer’ Tubers after Treatment with GA3

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 553
Author(s):  
Beata Janowska ◽  
Roman Andrzejak ◽  
Lidia Szwajkowska-Michałek ◽  
Kinga Stuper-Szablewska
Keyword(s):  
Antioxidant Activity ◽  
Gibberellic Acid ◽  
Caffeic Acid ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Coumaric Acid ◽  
Medicagenic Acid ◽  
Promising Source ◽  
Active Substances ◽  
Β Carotene

This study was conducted to assess the influence of gibberellic acid (GA3) on the content of biologically active substances in Crocosmia × crocosmiiflora ‘Lucifer’ tubers. These tubers are a promising source of potential antioxidants, and their extracts can be used in pharmaceutical industry, as well as in cosmetics products and antifungal and antibacterial substances. Four groups of biologically active substances were determined from tubers: saponins, phenolic acid, flavonoids and carotenoids. The antioxidant activity of the extracts from tubers increased proportionally to the GA3 concentrations. GA3 at concentrations of 200, 400 and 600 mg dm−3 increased the content of medicagenic acid by 42.9–57.1% and the content of polygalic acid by 50% without affecting the content of medicagenic acid 3-O-triglucoside. The GA3 concentrations used in the experiment positively influenced the accumulation of caffeic acid, p-coumaric acid and gallic acid. The highest content of caffeic acid was noted in the tubers soaked in GA3 concentrated at 400 and 600 mg dm−3. GA3 at a concentration used in the study stimulated the accumulation of kaempferol by 15%, quercetin by 7–8.2%, quercetin 3-O-glucoside by 1.8% (when GA3 was applied at a concentration of 200 mg·dm−3) and by 4.1% and 3.6% (when GA3 was applied at concentrations of 400 and 600 mg·dm−3) and kaempferol 3-O-rhamnosylglucoside by 1.5–3.4%. The soaking of the tubers in GA3 increased the content of β-carotene by 7.9%, 5.2% and 7.9%, respectively, without affecting the content of crocin. For soaking of Crocosmia tubers, it is recommended to use GA3 at a concentration of 400–600 mg·dm−3.

scholarly journals COMPOSITION OF BIOLOGICALLY ACTIVE SUBSTANCES OF THE HORSE CHESTNUT (AECULUS HIPPOCASTANUM L.)

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 33-39
Author(s):  
V.P. Kurchenko ◽  
◽  
N.V. Sushinskaya ◽  
K.I. Maiorava ◽  
E.I. Tarun ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Horse Chestnut ◽  
Hazard Class ◽  
Active Substances

The study of the composition of biologically active substances, alcoholic extracts from flowers of Aeculus hippocastanum L. According to the results of HPLC-MS and GC-MS analyzes, the extract contains the main amounts of phenolic compounds: quercetin, epicatechin, kaempferol. In addition, the extract contains fatty acids and their esters, alcohols, 3-deoxy-d-manno lactone, 1,2,3,5-cyclohexantethrol, α-methyl-mannofuranoside, γ sitosterol. Antioxidant activity of an extract from flowers of A. hippocastanum is associated with the peculiarities of the compositionof biologically active substances. The toxological-hygienic assessment of flowers of this species in acute and subacute experiments showed that it belongs to the 4th hazard class (low hazard).

scholarly journals Microalgae: A Promising Source of Valuable Bioproducts

Biomolecules ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1153 ◽  
Author(s):  
Vyacheslav Dolganyuk ◽  
Daria Belova ◽  
Olga Babich ◽  
Alexander Prosekov ◽  
Svetlana Ivanova ◽  
...  
Keyword(s):  
New Technologies ◽  
Value Added ◽  
Component Composition ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
High Productivity ◽  
Advantages And Disadvantages ◽  
Valuable Compounds ◽  
Promising Source ◽  
Active Substances

Microalgae are a group of autotrophic microorganisms that live in marine, freshwater and soil ecosystems and produce organic substances in the process of photosynthesis. Due to their high metabolic flexibility, adaptation to various cultivation conditions as well as the possibility of rapid growth, the number of studies on their use as a source of biologically valuable products is growing rapidly. Currently, integrated technologies for the cultivation of microalgae aiming to isolate various biologically active substances from biomass to increase the profitability of algae production are being sought. To implement this kind of development, the high productivity of industrial cultivation systems must be accompanied by the ability to control the biosynthesis of biologically valuable compounds in conditions of intensive culture growth. The review considers the main factors (temperature, pH, component composition, etc.) that affect the biomass growth process and the biologically active substance synthesis in microalgae. The advantages and disadvantages of existing cultivation methods are outlined. An analysis of various methods for the isolation and overproduction of the main biologically active substances of microalgae (proteins, lipids, polysaccharides, pigments and vitamins) is presented and new technologies and approaches aimed at using microalgae as promising ingredients in value-added products are considered.

scholarly journals Spelt (Triticum aestivum ssp. Spelta)– from Field to Cosmetics

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Georgieva-Krasteva L. ◽  
Hristova I. ◽  
Mihaylova D. ◽  
Dobreva K.
Keyword(s):  
Antioxidant Activity ◽  
Triticum Aestivum ◽  
Skin Penetration ◽  
Biologically Active ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Physicochemical Stability ◽  
Pleasant Odor ◽  
Promising Source ◽  
Active Substances

Plants have been intensively used as sources of biologically active compounds. Recently, the attention was directed to spelt (Triticum aestivum ssp. spelta) as a promising source of antioxidant compounds. In the present study, the antioxidant activity and total phenolic content of Triticum spelta glycerin extracts were determined. Three different extraction techniques were performed – conventional, microwave- and ultrasound- assisted extractions. Best polyphenol content and antioxidant activity were resulted when heat reflux extraction was applied. Moreover, nourishing and regenerating cream (NRC) formulation was prepared by incorporating 1 % glycerin spelt extract. The physicochemical stability of this formulation was assessed as well as sensory and skin penetration caracteristics.The cosmetic preparation exhibited good emulsion and colour stability at all temperature tested (-10 °C; 25 °C and 40 °C) during 90 days. 85 % of volunteers assessed the skin penetration of NRC as good. The same percentage claimed pleasant odor caracteristic after application on skin. Based on these results it can be concluded that spelt is a promissing source of biologically active substances with various applications.

scholarly journals Valorization of Lavender Waste – Obtaining and Characteristics of Polyphenol Rich Extracts

2018 ◽  
Vol 1 (1) ◽  
pp. 11 ◽  
Author(s):  
Kostadinka B. Karneva ◽  
Ivelina N. Vasileva ◽  
Petko N. Denev ◽  
Rositsa S. Denkova ◽  
Vasil T. Shikov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Dry Matter ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Linalyl Acetate ◽  
Lower Quantity ◽  
Promising Source ◽  
First Time ◽  
Active Substances

Bulgaria became the leading producer of lavender oil in the last years. Due to the lower quantity of essential oil large amounts of waste were generated which distilleries usually discard, although the residues are rich source of biologically active substances. The objective of this study was to obtain polyphenol-rich extracts from lavender waste and to investigate their chemical composition and antioxidant capacity. Two wastes (Mirkovo, Bulgaria, 2016) were investigated – steam distilled (SD-L) and CO2-extracted lavender (CO2-L). The major aroma constituents found were linalool and linalyl acetate – 30.68% and 25.82%, respectively, and the highest concentrations were found in the SD-L. The total flavonoids in CO2-L and SD-L were 2.91±0.11 and 3.72±0.20 mg/g dry matter residue, respectively. The higher amount of phenolic acids was observed in the SD-L waste – 2.62±0.19 mg/g, compared to 1.39±0.14 mg/g dry matter residue for CO2 extracted lavender. The antioxidant activity of the extracts was investigated by DPPH (SD-L – 355.48±23.12 µmol TE/g DW waste; CO2-L – 283.21±17.04 µmol TE/g DW waste) and FRAP (SD-L – 427.36±26.54 µmol TE/g DW waste; CO2-L – 311.29±18.17 µmol TE/g DW waste). Both methods suggested that SD-L residue had higher antioxidant capacity and polyphenol content.The lavender wastes (SD-L and CO2-L) showed strong antioxidant capacity with potential beneficial effect on addition in foodstuffs. For the first time lavender residues from CO2-extraction was investigated for its antioxidant activity, polyphenol composition and aroma metabolites, and comparison with SD-L was performed. In general, the results suggested that the lavender waste were promising source of antioxidants.

scholarly journals Biotechnological aspects of obtaining biologically active substances of Adonis vernalis

2021 ◽  
Vol 4 (1) ◽  
pp. 126-130
Author(s):  
V. R. Hamada ◽  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Raw Materials ◽  
Callus Cultures ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
In Vitro Cultivation ◽  
Plant Raw Materials ◽  
Active Substances

This article shows the possibility of using a biotechnological method to obtain biologically active compounds based on in vitro cultivation of callus cultures. The callus biomass of Adonis vernalis was obtained in vitro by using the biotechnological method. The extracts based on callus biomass of Adonis vernalis were obtained. The content of flavonoids and phenolic compounds was determined. The antioxidant activity of callus biomass of Adonis vernalis has also been studied. The research results show that the content of biologically active substances and biological activity in callus biomass does not differ from plant raw materials.

ECDYSTEROIDS AND FLAVONOIDS OF SILENE GRAEFFERI

2021 ◽  
pp. 175-182
Author(s):  
Elena Sergeyevna Filonenko ◽  
Larisa Nikolayevna Zibareva
Keyword(s):  
Aerial Part ◽  
Western Siberia ◽  
Botanical Garden ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
General Tendency ◽  
Vegetative Development ◽  
Promising Source ◽  
First Time ◽  
Active Substances

In this paper Silene graefferi Guss. is recommended for the first time as a promising source of flavonoids and is a well-known producer of ecdysteroids. This perennial species is adapted to the conditions of Western Siberia and has been successfully cultivated in the Siberian Botanical garden of TSU since 2015. It was interesting to study the composition and features of accumulation of biologically active substances during the growth and development of S. graefferi. It was shown that 20-hydroxyecdysone and polypodine B are present in the aerial and underground parts of introduced in culture S. graefferi plants during the entire vegetative development. An unidentified ecdysteroid was detected in the aerial part at the beginning of the vegetation and in the roots during the flowering phase. During vegetative development, common flavonoids and characteristic ones at different periods of plant development were found. The phase of maximum accumulation of ecdysteroids and flavonoids of S. graefferi is the beginning of vegetation, and the minimum is flowering. In the age dynamics the highest levels of biologically active substances in plants were noted at 2 and 3 years of life, then there is a general tendency to decrease with the age of the plant. From the aerial part of S. graefferi, 20-hydroxyecdysone, polypodine B and vitexin were isolated and identified by HPLC.

scholarly journals The use of ultrasonic extraction in the technology of functional drinks based on plant raw materials

2021 ◽  
Vol 845 (1) ◽  
pp. 012114
Author(s):  
M V Manukovskaya ◽  
I P Shchetilina ◽  
Y N Pisklyukova ◽  
E A Klimova ◽  
M I Korystin
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Human Body ◽  
Raw Materials ◽  
Ultrasonic Extraction ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Plant Raw Materials ◽  
Organoleptic Characteristics ◽  
Active Substances

Abstract The development of targeted drinks is a priority in the field of healthy eating. For their production, plant raw materials are actively used, containing a wide variety of biologically active substances. The presence of these ingredients helps to improve many physiological processes in the body, as well as to increase its immune status. Numerous studies have confirmed the high efficiency of ultrasonic effects on various technological processes. Ultrasonic extraction was carried out at t = 20 °C and an ultrasonic wave frequency of 22 kHz. As a result of research, it was found that with ultrasound exposure, an equilibrium state is achieved within 15 minutes. In order to develop new recipes for drinks, natural raw materials, their chemical composition and the effects on the human body have been studied. The selected ingredients in a certain quantitative ratio made it possible to obtain drinks with improved bio-corrective properties, which allows satisfying the daily requirement of the human body for biologically active substances. Ultrasonic exposure has a positive effect on the organoleptic characteristics of the drink and the aftertaste, as evidenced by the comparative organoleptic evaluation of the samples. The functional properties of the developed drinks have been confirmed experimentally. In the drinks, the following were determined: the content of macronutrients, antioxidant activity, the content of vitamin C, the duration of the shelf life. The use of the method of ultrasonic extraction in the technology of preparing drinks allows one: to reduce the preparation time by half, to reduce the amount of raw materials by 15 %, to increase the content of macronutrients by 45 %, to increase the content of vitamin C by 35 %, to increase the antioxidant activity by 45-50 %, increase stability during storage by 50 – 60 %, increase organoleptic characteristics. The results obtained prove the intensifying effect of ultrasonic exposure in the production of functional drinks.

scholarly journals Panax ginseng callus, suspension, and root cultures: extraction and qualitative analysis

2020 ◽  
Vol 8 (2) ◽  
pp. 369-376
Author(s):  
Lyubov Dyshlyuk ◽  
Anastasia Dmitrieva ◽  
Svetlana Ivanova ◽  
Yuliya Golubtsova ◽  
Lev Ostroumov
Keyword(s):  
Qualitative Analysis ◽  
Organic Compounds ◽  
Panax Ginseng ◽  
Callus Cultures ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Root Cultures ◽  
Optimal Parameters ◽  
Promising Source ◽  
Active Substances

Introduction. In recent years, scientists have been actively searching for medicinal plants containing biologically active substances with geroprotective properties to treat diseases of old age, in particular cancer, diabetes, cardiovascular diseases, and others. Ginseng (Panax ginseng L.) is a promising source of geroprotective compounds. We aimed to select optimal parameters for extracting organic compounds from ginseng callus, suspension, and root cultures and analyze their qualitative composition. Study objects and methods. We studied ginseng callus, suspension, and root cultures, as well as their extracts. Biologically active substances were extracted with 30 to 70% ethanol. Organic compounds were determined by thin-layer chromatography. The results for each plant were archived and analyzed for the presence of quercetin, mangiferin, luteolin, rutin, quercetin-2-D-glucoside, malvidin, as well as caffeic, cinnamic, ferulic, and sinapinic acids. Results and discussion. We developed a procedure for screening solvents and performed a fractional qualitative analysis of biologically active substances extracted from ginseng. As a result, we established the optimal parameters for extracting biologically active substances from the dried biomass of ginseng cultures. In all cases, temperature and the ratio of solvent to biomass were the same (50°C, 1:5). However, the extraction time and ethanol concentration differed, amounting to 60 min and 50% for callus cultures, 30 min and 60% for suspension cultures, and 60 min and 70% for root cultures. The qualitative analysis of organic compounds showed the presence of rutin (0.25), quercetin (0.75), and mangiferin (0.57), as well as caffeic and sinapinic acids in the extracts. Conclusion. Our set of experiments to isolate biologically active substances from ginseng callus, suspension, and root cultures resulted in selecting the optimal extraction parameters and analyzing the extracts for the presence of organic compounds.

scholarly journals Optimization of Extraction of Polyphenolic Compounds from Medicinal Lungwort (Pulmonaria officinalis L.)

2020 ◽  
pp. 36-45
Author(s):  
Lyubov S. Dyshlyuk ◽  
Anastasia M. Fedorova ◽  
Vyacheslav F. Dolganyuk ◽  
Alexander Yu. Prosekov
Keyword(s):  
Antioxidant Activity ◽  
Alcohol Concentration ◽  
Polyphenolic Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
High Yield ◽  
Operating Parameters ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Active Substances

Medicinal plants are potential natural sources of biologically active substances that have geroprotective properties, slowing down the aging process. Vegetable raw materials are used as antioxidants in the food industry. Pulmonaria officinalis L. has a high content of biologically active substances. The purpose of this work is to determine the operating parameters for extracting the maximum amount of biologically active substances from Pulmonaria officinalis L. by extracting ethanol and finding sources of natural antioxidants.The antioxidant activity of several ethanol extracts of Pulmonaria officinalis L. – 30-70% with a step of 10 ethanol obtained from the dried root culture of the medicinal plant of biologically active substances was determined. The total content of polyphenols, flavonoids and proanthocyanidins was estimated. According to the data obtained, the relationship between the values determined for the biologically active substances presented and the values of antioxidant activity was revealed. Extracts of Pulmonaria officinalis L. showed a high yield of polyphenolic compounds-889.39±4.29 mcg of Gallic acid/ml, flavonoids-728.90±6.98 mcg of rutin/ml, proanthocyanidins-211.65±5.31 mcg of catechin/ml at an ethyl alcohol concentration of 60% and an extraction temperature of 50°C in 4 hours. The antioxidant activity using 2 methods: A spectrophotometric method DPPH (2,2-diphenyl-1-picrylhydrazyl) and the FRAP method (iron reducing/antioxidant power) was investigated. During DPPH spectrophotometric analysis, the antioxidant activity is equal to 86.96% for polyphenols, 75.47% for flavonoids, and 51.25% for proanthocyanidins. FRAP analysis showed that extracts with operating parameters had a pronounced antioxidant activity (tэ= 4 ч; Tэ= 50°C; Сэ= 60%)и (tэ= 5 ч; Tэ= 60°C; Сэ, = 70%).

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