scholarly journals Exogenous Foliar Application of Glycine Betaine to Alleviate Water Deficit Tolerance in Two Indica Rice Genotypes under Greenhouse Conditions

Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 138 ◽  
Author(s):  
Rujira Tisarum ◽  
Cattarin Theerawitaya ◽  
Thapanee Samphumphung ◽  
Teruhiro Takabe ◽  
Suriyan Cha-um

The aim of this investigation was to enhance overall growth, yield attributes as well as physio-biochemical adaptive strategies by exogenous foliar application of glycine betaine (GlyBet) in two rice varieties against water deficit stress under greenhouse conditions. Rice crop cvs. RD43 (low amylose content) and SPR1 (high amylose content) grown in clay pots containing garden soil until booting stage were chosen as the test plant material, sprayed by 0 (control) or 100 mM GlyBet and subsequently subjected to: MWD (mild water deficit by 8 d water withholding; 24.80% SWC; Soil water content) or SWD (severe water deficit by 14 d water withholding; 13.63% SWC) or WW (well-watered conditions or control). Free proline content in cv. RD43 was rapidly increased in relation to the degree of water deficit and suppressed by exogenous GlyBet, while free proline in cv. SPR1 was lower than cv. RD43. Overall growth performances and yield traits in both cultivars under MWD were maintained by exogenous application of GlyBet; however, these parameters declined under SWD even after the GlyBet application. Degradation of photosynthetic pigments and chlorophyll fluorescence in pretreated GlyBet plants under SWD were prevented, resulting in elevated net photosynthetic rate (Pn). Interestingly, Pn was very sensitive parameter that sharply declined under SWD in both RD43 and SPR1 genotypes. Positive relationships between physio-morphological and biochemical changes in rice genotypes were demonstrated with high correlation co-efficiency. Based on the key results, it is concluded that foliar GlyBet application may play an important role in drought-tolerant enhancement in rice crops.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. A. Gomaa ◽  
Essam E. Kandil ◽  
Atef A. M. Zen El-Dein ◽  
Mamdouh E. M. Abou-Donia ◽  
Hayssam M. Ali ◽  
...  

AbstractIn Egypt, water shortage has become a key limiting factor for agriculture. Water-deficit stress causes different morphological, physiological, and biochemical impacts on plants. Two field experiments were carried out at Etay El-Baroud Station, El-Beheira Governorate, Agriculture Research Center (ARC), Egypt, to evaluate the effect of potassium silicate (K-silicate) of maize productivity and water use efficiency (WUE). A split-plot system in the four replications was used under three irrigation intervals during the 2017 and 2018 seasons. Whereas 10, 15, and 20 days irrigation intervals were allocated in main plots, while the three foliar application treatments of K-silicate (one spray at 40 days after sowing; two sprays at 40 and 60 days; and three sprays at 40, 60, and 80 days, and a control (water spray) were distributed in the subplots. All the treatments were distributed in 4 replicates. The results indicated that irrigation every 15 days gave the highest yield in both components and quality. The highly significant of (WUE) under irrigation every 20 days. Foliar spraying of K-silicate three times resulted in the highest yield. Even under water-deficit stress, irrigation every fifteen days combined with foliar application of K-silicate three times achieved the highest values of grain yield and its components. These results show that K-silicate treatment can increase WUE and produce high grain yield requiring less irrigation.


2017 ◽  
Vol 9 (12) ◽  
pp. 259
Author(s):  
Amin Namdari ◽  
Abolfazl Baghbani

Due to low rainfall at early autumn, smooth vetch seedling growth in rain-fed lands often is limited by water deficit stress yet the data regarding the reactions of smooth vetch to water deficit at early growth stages are pretty rare. The objective of current study was to examine possibility of using priming treatments (hydro priming and priming salicylic acid) to alleviate the inhibitory effect of water deficiency during early growth of Smooth Vetch. In this respect, seeds were soaked in distilled water (hydro priming) or 0.5 mM solution of SA for 36 h at 10 °C then dried back to original moisture content. Pots were irrigated for 25 days at four levels of available water containing field capacity (FC), 75% FC, 50% FC and 25% FC. In general, seedling emergence and early growth were markedly limited by increasing water deficiency. However, priming treatments particularly with SA caused considerable improvement in either emergence or growth of seedlings (dry weight, length). The obtained results showed that primed samples exhibited higher accumulation of proline, glycine betaine (GB) under all levels of available water except 100% FC and also higher total soluble sugars (TSS) and trehalose under severe water deficit (25% FC). SA primed samples had higher relative water content especially under higher levels of water deficiency. The more balanced water status within SA primed samples also was accompanied with higher accumulation of proline and glycine betaine. There were significant differences between two priming treatments in terms of proline and GB content within seedlings and SA priming considerably increased proline and GB accumulation. In contrast to proline and GB, TSS and trehalose content wasn’t influenced by SA treatment and both hydro and SA primed samples showed statistically similar quantities.


2012 ◽  
Vol 4 (1) ◽  
pp. 112-115 ◽  
Author(s):  
Hossein MARDANI ◽  
Hassan BAYAT ◽  
Amir Hossein SAEIDNEJAD ◽  
Ehsan Eyshi REZAIE

Impacts of various concentrations of salicylic acid (SA) on cucumber (Cucumis sativus L.) seedling characteristic were evaluated under different water stress levels by using a factorial arrangement based on completely randomized design with three replications at experimental greenhouse of Ferdowsi University of Mashhad, Iran. The studied factors included three water deficit levels (100% FC, 80% FC, and 60% FC) considered as first factor and five levels of SA concentrations (0, 0.25, 0.5, 0.75, and 1 mM) as second factor. Results showed that foliar application of SA at the highest concentration enhanced leaf area, leaf and dry weight while decreased stomatal conductance under high level of water deficit stress. Though, severe water deficit stress sharply raised the SPAD reading values. In general, exogenous SA application could develop cucumber seedling characteristic and improve water stress tolerance.


Agriculture ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 392
Author(s):  
Hafiz Ghulam Muhu-Din Ahmed ◽  
Muhammad Sajjad ◽  
Yawen Zeng ◽  
Muhammad Iqbal ◽  
Sultan Habibullah Khan ◽  
...  

The decrease in water resources is a serious threat to food security world-wide. In this regard, a genome-wide association study (GWAS) was conducted to identify grain yield and quality-related genes/loci under normal and water-deficit conditions. Highly significant differences were exhibited among genotypes under both conditions for all studied traits. Water-deficit stress caused a reduction in grains yield and an increase in grains protein contents (GPC) and gluten contents (GLC). Population structure divided the 96 genotypes into four sub-populations. Out of 72 significant marker-trait associations (MTAs), 28 and 44 were observed under normal and water-deficit stress conditions, respectively. Pleiotropic loci (RAC875_s117925_244, BobWhite_c23828_341 and wsnp_CAP8_c334_304253) for yield and quality traits were identified on chromosomes 5A, 6B and 7B, respectively, under normal conditions. Under a water-deficit condition, the pleiotropic loci (Excalibur_c48047_90, Tdurum_contig100702_265 and BobWhite_c19429_95) for grain yield per plant (GYP), GPC and GLC were identified on chromosomes 3A, 4A and 7B, respectively. The pleiotropic loci (BS00063551_51 and RAC875_c28721_290) for GPC and GLC on chromosome 1B and 3A, respectively, were found under both conditions. Besides the validation of previously reported MTAs, some new MTAs were identified for flag leaf area (FLA), thousand grain weight (TGW), GYP, GPC and GLC under normal and water-deficit conditions. Twenty SNPs associated with the traits were mapped in the coding DNA sequence (CDS) of the respective candidate genes. The protein functions of the identified candidate genes were predicted and discussed. Isolation and characterization of the candidate genes, wherein, SNPs were mapped in CDS will result in discovering novel genes underpinning water-deficit tolerance in bread wheat.


2019 ◽  
Vol 89 (1) ◽  
pp. 109-118 ◽  
Author(s):  
Nanbo Wang ◽  
Fangbin Cao ◽  
Marvin Eusi Ambrose Richmond ◽  
Chengwei Qiu ◽  
Feibo Wu

2019 ◽  
Vol 56 (Special Issue) ◽  
pp. 92-105
Author(s):  
Rabi N Sahoo ◽  
C Viswanathan ◽  
Gopal Krishna ◽  
Bappa Das ◽  
Swati Goel ◽  
...  

Present paper deals with different components of next generation phenomics for characterizing rice genotypes for water deficit stress. Major sensors used in the study were non-imaging hyperspectal remote sensing, thermal imaging at ground platform and RGB and multispectral imaging sensors from drone platform. Different spectral indices were evaluated along with new proposed index and different multivariate models were studied for non-invasive estimation of relative water content (RWC) and sugar content in rice plant using spectral reflectance data collected in spectral range 350 to 2500 nm. Spectral data were further used for spectral discrimination of rice genotypes. Crop water stress index derived from thermal images acquired for rice genotypes could well characterize the drought resistant and sensitive genotypes. Initial study on field phenotyping through drone remote sensing using multispectral and RGB sensor was also explored to capture differential response of genotypes, trait and heat map mapping. All developed protocols as reliable alternative to conventional methods are fast, economic and non-invasive and in use in plant phenomics centre for high throughput plant phenotyhping for water deficit stress studies.


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