Supplement Based on Fermented Milk Permeate for Feeding Newborn Calves: Influence on Blood, Growth Performance, and Faecal Parameters, including Microbiota, Volatile Compounds, and Fatty and Organic Acid Profiles

The aim of this study was to assess the effect of a feed supplement, namely milk permeate (MP) fermented with Lactobacillus uvarum LUHS245, on the newborn calves’ growth performance and blood and faecal parameters, including microbiota and volatile compound and fatty acid profiles. Ten female Holstein calves in the control group (CON group) were fed with a standard milk replacer diet and colostrum only, from day 2 to 14 of life, while 10 calves of the treated group (MP group) were fed with the same diet supplemented with 50 mL of the fermented MP. After 14 days, there were no significant differences between the groups in blood parameters, growth performance, or faecal pH. There was a significantly higher percentage of live lactic acid bacteria (by 17.02%), a lower percentage of enterobacteria (by 10.38%), a higher overall number of probiotic bacteria, a 1.7-fold higher species variety, and a higher content of dry matter in the faeces of the MP group (p < 0.05). The fatty acid and volatile compound profiles differed significantly between the groups. The results suggest that supplementing calves’ feed with fermented milk permeate has a positive effect on certain health parameters but not on blood parameters or growth performance.

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Supplementing Genistein for Breeder Hens Alters the Fatty Acid Metabolism and Growth Performance of Offsprings by Epigenetic Modification

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/9214209 ◽

2019 ◽

Vol 2019 ◽

pp. 1-15 ◽

Cited By ~ 5

Author(s):

Zengpeng Lv ◽

Hao Fan ◽

Bochen Song ◽

Guang Li ◽

Dan Liu ◽

...

Keyword(s):

Fatty Acid ◽

Lipid Metabolism ◽

Growth Performance ◽

Fatty Acid Biosynthesis ◽

Cholesterol Metabolism ◽

Epigenetic Modification ◽

Treated Group ◽

Control Group ◽

Histone H4 ◽

Acyl Coenzyme A

The experiment was designed to clarify the effect and molecular mechanism of maternal genistein (GEN) on the lipid metabolism and developmental growth of offspring chicks. Laying broiler breeder (LBB) hens were supplemented with 40 mg/kg genistein (GEN), while the control group was fed with the low-soybean meal diet. The offspring chicks were grouped according to the mother generation with 8 replicates each. Hepatic transcriptome data revealed 3915 differentially expressed genes (DEGs, P adjusted < 0.05, fold change>1.5 or fold change<0.67) between chicks in the two groups. Maternal GEN activated the GH-IGF1-PI3K/Akt signaling pathway, which promoted the developmental processes and cellular amino acid metabolic processes, as well as inhibited the apoptotic process. GEN treatment significantly increased the weight gain, breast muscle percentage, and liver index in chicks. PANTHER clustering analysis suggested that maternal GEN enhanced the antioxidant activity of chicks by the upregulation of gene (SOD3, MT1, and MT4) expression. Accordingly, the activities of T-AOC and T-SOD in the liver were increased after GEN treatment. The overrepresentation tests revealed that maternal GEN influenced the glycolysis, unsaturated fatty acid biosynthesis, acyl-coenzyme A metabolism, lipid transport, and cholesterol metabolism in the chick livers. Hepatic cholesterol and long-chain fatty acid were significantly decreased after GEN treatment. However, the level of arachidonic acid was higher in the livers of the GEN-treated group compared with the CON group. Moreover, GEN treatment enhanced fatty acid β-oxidation and upregulated PPARδ expression in the chick liver. ChIP-qPCR analysis indicated that maternal GEN might induce histone H3-K36 trimethylation in the promoter region of PPARδ gene (PPARD) through Iws1, methyltransferases. It also induced histone H4-K12 acetylation at the PPARD promoter through MYST2, which activated the PPAR signaling pathways in the chick livers. In summary, supplementing LBB hens with GEN can alter lipid metabolism in the offspring chicks through epigenetic modification and improve the antioxidative capability as well as growth performance.

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Effects of Replacing Yellow Corn with Olive Cake Meal on Growth Performance, Plasma Lipid Profile, and Muscle Fatty Acid Content in Broilers

Animals ◽

10.3390/ani11082240 ◽

2021 ◽

Vol 11 (8) ◽

pp. 2240

Author(s):

Ahmed Saleh ◽

Mohammed Alzawqari

Keyword(s):

Fatty Acid ◽

Lipid Profile ◽

Growth Performance ◽

Fatty Acid Content ◽

Plasma Lipid ◽

Abdominal Fat ◽

Control Group ◽

Plasma Lipid Profile ◽

Olive Cake ◽

Arachidonic Acids

The current study focused exclusively on evaluating the effects of replacing corn with olive cake meal (OCM) in the diet of broilers on their growth performance, abdominal fat, selected plasma parameters, and muscle fatty acid (FA) content. A total of 480 one-day-old male broiler chickens (Ross 308) were divided into four treatment groups with 12 replicates/treatment. The control group was fed the base diet, whereas the second to fourth groups were fed diets of corn with 5%, 10%, and 20% contents of OCM, respectively. Broilers fed with the 5% and 10% OCM diets showed better body weight (p = 0.04) and feed conversion ratio than the 20% OCM group (p < 0.048). Both nitrogen retention and ether extract digestibility were not improved by replaced corn with OCM. Replacing corn with OCM led to a decreased abdominal fat percentage (p = 0.023) compared with the control group. Birds in the OCM groups showed the lowest total cholesterol values (p = 0.038). The breast muscle (musculus pectoralis superficialis) content of oleic and linoleic, linolenic, and arachidonic acids was significantly high in birds fed with OCM diets. However, their palmitic acid level was significantly decreased. Vitamin E was increased by increasing the OCM level. Thus, we concluded that replacing corn with OCM, especially at a 10% level, is more effective than other replacement levels in improving growth performance, plasma lipid profile, and muscle FA content, as well as in causing a reduction in abdominal fat in broilers.

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Effects of Rocket Seed Oil, Wheat Germ Oil, and Their Mixture on Growth Performance, Feed Utilization, Digestibility, Redox Status, and Meat Fatty Acid Profile of Growing Rabbits

Agriculture ◽

10.3390/agriculture11070662 ◽

2021 ◽

Vol 11 (7) ◽

pp. 662

Author(s):

Sabrin Abdelrahman Morshedy ◽

Ahmed M. Abdelmodather ◽

Mohamed M. Basyony ◽

Soliman A. Zahran ◽

Mohamed A. Hassan

Keyword(s):

Immune Response ◽

Fatty Acid ◽

Growth Performance ◽

Wheat Germ ◽

Seed Oil ◽

Average Daily Gain ◽

Feed Utilization ◽

Nutrient Digestibility ◽

Control Group ◽

Wheat Germ Oil

Vegetable oils are a source of natural antioxidants, including tocopherols, sterols, phenolic compounds, coenzymes, and polyunsaturated fatty acids that provide nutritional value, organoleptic properties, and significantly delay or prevent lipid oxidation. Eighty-four V-line rabbits at 5 weeks of age with an initial body weight (BW) of 535.60 ± 13.48 g were assigned randomly to four experimental groups (seven replicates in each group with three rabbits each). The first group served as a control and received 0.3 mL/kg BW of distilled water (CON), while the second and third groups received 0.3 mL/kg BW of rocket seed oil (RSO) and wheat germ oil (WGO), respectively. The fourth group received a mixture of oils consisting of 0.15 mL of RSO and 0.15 mL of WGO/kg BW (MOs). The experiment lasted 7 weeks. The study investigated the effects of RSO, WGO, and their mixture on growth performance, feed utilization, antioxidant status, and immune response of growing rabbits. The results indicated that the rabbits that were administered orally with RSO and WGO or their mixture had higher (p ≤ 0.05) final BW, weight gain, and average daily gain when compared to the control group. In addition, the feed conversion ratio improved significantly with RSO, WGO, and MOs treatments. Different oil treatments improved nutrient digestibility, nutritive value, and nitrogen balance. Moreover, the rabbits that received RSO, WGO, and their mixture had an improvement the meat fatty acid composition compared to the control rabbits. Oral administration of RSO, WGO, and their mixture significantly improved serum protein fractions, decreased blood urea nitrogen, and had a positive effect on serum total lipids, HDL-c, and LDL-c. Furthermore, the treatments of RSO, WGO, and MOs had a significant improvement in the antioxidative status and immune response.

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Effect of a Rumen-Protected Microencapsulated Supplement from Linseed Oil on the Growth Performance, Meat Quality, and Fatty Acid Composition in Korean Native Steers

Animals ◽

10.3390/ani11051253 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1253

Author(s):

Chae-Hyung Sun ◽

Jae-Sung Lee ◽

Jalil Ghassemi Nejad ◽

Won-Seob Kim ◽

Hong-Gu Lee

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Growth Performance ◽

Meat Quality ◽

Acid Composition ◽

Linseed Oil ◽

Control Group ◽

Ruminal Fluid

We evaluated the effects of a rumen-protected microencapsulated supplement from linseed oil (MO) on ruminal fluid, growth performance, meat quality, and fatty acid composition in Korean native steers. In an in vitro experiment, ruminal fluid was taken from two fistulated Holstein dairy cows. Different levels of MO (0%, 1%, 2%, 3%, and 4%) were added to the diet. In an in vivo experiment, eight steers (average body weight = 597.1 ± 50.26 kg; average age = 23.8 ± 0.12 months) were assigned to two dietary groups, no MO (control) and MO (3% MO supplementation on a DM basis), for 186 days. The in vitro study revealed that 3% MO is an optimal dose, as there were decreases in the neutral detergent fiber and acid detergent fiber digestibility at 48 h (p < 0.05). The in vivo study showed increases in the feed efficiency and average daily gain in the 3% MO group compared to the control group on days 1 to 90 (p < 0.05). Regarding meat quality, the shear force produced by the longissimus thoracis muscle in steers from the 3% MO group was lower than that produced by the control group (p < 0.05). Interestingly, in terms of the fatty acid profile, higher concentrations of C22:6n3 were demonstrated in the subcutaneous fat and higher concentrations of C18:3n3, C20:3n3, and C20:5n3 were found in the intramuscular fat from steers fed with 3% MO (p < 0.05). Our results indicate that supplementation with 3% MO supplements improves the growth performance and meat quality modulated by the omega-3 fatty acid content of meat in Korean native steers.

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Short communication: Growth performance, nutrient digestibility and blood parameters of fattening lambs fed diet replacing corn with orange pulp

Spanish Journal of Agricultural Research ◽

10.5424/sjar/2017151-9078 ◽

2017 ◽

Vol 15 (1) ◽

pp. e06SC01 ◽

Cited By ~ 1

Author(s):

Saman Lashkari ◽

Akbar Taghizadeh ◽

Hamid Paya ◽

Søren K. Jensen

Keyword(s):

Growth Performance ◽

Average Daily Gain ◽

Blood Parameters ◽

Rumen Fermentation ◽

Linear Increase ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Control Group ◽

Quadratic Effect ◽

Orange Pulp

The objective of the present study was to evaluate the effect of replacing corn with orange pulp (OP) on growth performance, rumen fermentation, nutrient digestibility and blood parameters of fattening lambs. Twenty male lambs were placed in individual pens and fed with four levels of replacement of corn by OP (0, 33, 66, 100%) during 60 days. Average daily gain (ADG) showed a quadratic effect (p<0.007) with the increasing levels of replacement. Inclusion of 33 and 66% of OP in the diet significantly increased dry matter intake (DMI) compared to control group (p<0.01). Ruminal ammonia-N concentration showed a linear decrease (p<0.002). Ruminal fluid pH increased linearly with the increasing replacement of corn by OP (p<0.001). Acetate concentration showed a linear increase (p<0.001). Plasma total protein showed a linear increase (p<0.002). Organic matter, crude protein and neutral detergent fiber showed a quadratic effect with the level of replacement. The results of the present study showed that replacement of corn by OP improves DMI of fattening lambs, leading to an enhancement in ADG at the replacement level of 40.3%. Also, total replacement of corn by OP did not have any adverse effect on growth performance, rumen fermentation, nutrient digestibility and blood parameters.

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85 REGULATION OF STEAROYL-CoENZYME A DESATURASE BY FATTY ACIDS IS ESSENTIAL TO PORCINE EARLY EMBRYO DEVELOPMENT

Reproduction Fertility and Development ◽

10.1071/rdv29n1ab85 ◽

2017 ◽

Vol 29 (1) ◽

pp. 150

Author(s):

D.-K. Lee ◽

J. Y. Hwang ◽

K.-H. Choi ◽

S.-H. Kim ◽

J.-N. Oh ◽

...

Keyword(s):

Fatty Acids ◽

Oleic Acid ◽

Fatty Acid ◽

Stearic Acid ◽

Transcriptome Analysis ◽

Coenzyme A ◽

Formation Rate ◽

Treated Group ◽

Control Group ◽

Blastocyst Formation

Various fatty acids are found in large amounts in follicular fluid and the uterus. These fatty acids are known to regulate lipid metabolism in a mammal’s embryo. Lipid metabolism provides a source of energy, and its importance during embryogenesis is being increasingly recognised. Especially, the pig has larger amounts of intercellular lipid bilayers in the embryo than do other species, which indicates the porcine embryo is more dependent on fatty acid on their metabolic pathway. This study investigated the transcriptome analysis data of in vivo embryos and the effect of oleic acid (C18:1n-9) and stearic acid (C18:0) on in vitro-produced porcine embryos. In transcriptome analysis of in vivo embryos, we found several genes were increasing before and after maternal zygotic transition stage, and interactions were mapped and given a significance score. Among these genes, stearoyl-coenzyme A desaturase (SCD) gene was significantly increased during 8-cell to blastocyst stage. Stearoyl-coenzyme A desaturase is responsible for converting saturated fatty acid (stearic acid) to monounsaturated fatty acid (oleic acid). Furthermore, we treated with oleic acid (50, 100, 250, and 500 μM) and stearic acid (50, 75, and 100 μM) on 2 day and 4 day after parthenogenetic embryos. Both oleic acid and stearic acid concentration over 100 μM had a negative effect on blastocyst formation rate and cell numbers because of exogenous fatty acid toxicity. In addition, there was no significant difference among all stearic acid treated groups and total cell number of oleic acid treated blastocyst. However, the 2-day oleic acid treated group (45.92 ± 4.01, n = 8, 2 day, 100 μM) had a significant increase of blastocyst formation rate (P < 0.05) compared with the control group (34.88 ± 2.93, n = 8). These data could support that porcine embryos can use exogenous oleic acid as a metabolic energy source. The data also demonstrate the important role of SCD in porcine early embryo development. To confirm the transcriptome analysis, we are investigating mRNA level of SCD on in vitro-produced embryos at 1-cell to morula and blastocyst stage on the control group, oleic acid treated group, fetal bovine serum treated group, and nontreated group. Furthermore, we are investigating SCD inhibition assay by A939572 (SCD inhibitor) on parthenogenetic embryos in a fetal bovine serum treated group and nontreated group for identifying blastocyst formation rate and total cell numbers. This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2014R1A1A2055199) and Next BioGreen 21 program (PJ0113002016), Rural Development Administration, Republic of Korea.

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Effects of dietary chitosan on growth, lipid metabolism, immune response and antioxidant-related gene expression in Misgurnus anguillicaudatus

Beneficial Microbes ◽

10.3920/bm2016.0177 ◽

2017 ◽

Vol 8 (3) ◽

pp. 439-449 ◽

Cited By ~ 33

Author(s):

J. Yan ◽

C. Guo ◽

M.A.O. Dawood ◽

J. Gao

Keyword(s):

Fatty Acid ◽

Lipid Metabolism ◽

Growth Performance ◽

Antioxidant Status ◽

Body Weight Gain ◽

Control Group ◽

Dependent Manner ◽

Misgurnus Anguillicaudatus ◽

Fatty Acid Binding ◽

Expression Levels

This study was performed to evaluate the effects of dietary chitosan supplementation on growth performance, lipid metabolism, gut microbial, antioxidant status and immune responses of juvenile loach (Misgurnus anguillicaudatus). Five experimental diets were formulated to contain graded levels of chitosan (0 (control), 0.5, 1, 2 and 5% CHI) for 50 days. Results of the present study showed that body weight gain was significantly higher in fish fed chitosan supplemented diets in dose dependent manner than control group. Increasing dietary chitosan levels reduced gut lipid content. Meanwhile the mRNA expression levels of intestine lipoprotein lipase and fatty acid binding protein 2 were significantly reduced with incremental dietary chitosan level. The percentages of total monounsaturated fatty acid decreased, while polyunsaturated fatty acid increased with dietary chitosan. The fish fed 0.5% CHI had higher mucus lysozyme activity (LZM) than those fed 0% CHI, but the LZM activity was significantly decreased with advancing chitosan supplement. The expression levels of superoxide dismutase, catalase and glutathione peroxidase revealed a similar trend, where the highest expressions were found in fish fed 5% CHI diet. In the term of intestine microbiota between 0 and 1% CHI groups, the proportion of bacteria in the phylum Bacteroidetes increased, whereas the proportion of bacteria in the phylum Firmicutes decreased as the fish supplemented chitosan. In conclusion, supplementation of chitosan improved growth performance, antioxidant status and immunological responses in loach.

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Combination of Xylan Depolymerizing and Debranching Enzymes Improves Digestion, Growth Performance and Intestinal Volatile Fatty Acid Profile of Piglets

10.20944/preprints202108.0198.v1 ◽

2021 ◽

Author(s):

Weiwei Wang ◽

Dawen Zheng ◽

Zhenzhen Zhang ◽

Qingyun Cao ◽

Hui Ye ◽

...

Keyword(s):

Fatty Acid ◽

Growth Performance ◽

Fatty Acid Profile ◽

Volatile Fatty Acid ◽

Feruloyl Esterase ◽

Control Group ◽

In Vitro Digestibility ◽

Final Body Weight ◽

Debranching Enzymes

This study was aimed to investigate the effect of xylan depolymerizing enzyme namely endo-xylanase (Xyn) combined with debranching enzymes namely arabinofuranosidase (Afd) and feruloyl esterase (FE) on digestion, growth performance and intestinal volatile fatty acid profile of piglets. The in vitro experiments were firstly conducted to examine the enzymological properties of Xyn, Afd and FE, the synergy among these enzymes, together with the effect of combination of these enzymes on digestion of piglet diet. The in vivo experiment was then implemented by allocating 270 35-d-old postweaning piglets into 3 treatment groups: control group, Xyn group and (Xyn+Afd+FE) group. Each group had 6 replicates (15 piglets/replicate). The results revealed a satisfying thermostability and pH stability of Xyn, Afd and FE. Combination of Xyn, Afd and FE had a superiority (P < 0.05) over Xyn alone and its combination with Afd or FE in promoting degradation of different bran fibers rich in arabinoxylan (Abx). Treatment with combination of Xyn, Afd and FE had advantages over Xyn alone to induce increasing trends (P < 0.10) of in vitro digestibility of dietary nutrients (dry matter, crude protein, crude ash and gross energy) and piglet growth performance (average daily gain, final body weight and feed efficiency), concurrent with a reduction (P < 0.05) of diarrhea rate and increases (P < 0.05) in cecal acetic acid, butyric acid and total volatile fatty acids concentrations as well as pH value of piglets. Collectively, combination of Xyn, Afd and FE was efficient in benefiting degradation of Abx in brans, as well as improving digestion, growth performance and intestinal volatile fatty acid profile of piglets.

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Impact of Siberian larch dihydroquercetin or dry distilled rose petals as feed supplements on 8 lamb's growth performance, carcass characteristics and blood count parameters.

10.31220/agrirxiv.2020.00006 ◽

2020 ◽

Author(s):

N. Zh. Stancheva ◽

J. L. Nakev ◽

D. B. Vlahova-Vangelova ◽

D. K. Balev ◽

S. G. Dragoev

Keyword(s):

Growth Performance ◽

Blood Count ◽

Siberian Larch ◽

Live Weight ◽

Blood Parameters ◽

Control Group ◽

Rose Petals ◽

The Impact ◽

Group D ◽

Experimental Group

Abstract The objective of this study was to determine the impact of dihydroquercetin from Siberian larch and dry distilled rose petals (DDRP) on growth performance, carcasses characteristics and blood parameters of lambs from the Bulgarian Dairy Synthetic population sheep. For the purpose of the study there were used 30 clinically healthy male lambs aged 65 days, levelled by live weight. They were housed in a totally indoor barn and were divided into one control and two experimental groups, each consisting of 10 animals that were fed for 50 days. The control group (C) was fed ground alfalfa + granulated compound feed. The experimental groups (D) and (R) were fed on the same diet supplemented either with 7.5 mg dihydroquercetin/kg/day or with 545 mg DDRP/kg/day respectively. The carcass yield of lambs having consumed dihydroquercetin or DDRP compared to lambs from control group C do not have significant differences (p > 0.05). The carcass conformation of C or R groups lambs do not differ (70% - class P, 30% - class O). The 90% of lamb's carcasses from the experimental group D were classified in class P. The dihydroquercetin feeding increases the relative fat content (p ≤ 0.01) of lamb carcasses but adversely affects their conformation. No significant differences (p > 0.05) were found between 1st h and 24th h post-mortem pH of control group C and experimental group D. Compared to them the pH values of the experimental group R were by 0.14-0.15 pH units lower (p ≤ 0.05). No significant differences (p > 0.05) were found in the blood count of the three studied groups of lambs. Exceptions were made for haemoglobin (HGL) in the experimental group D which were with 6-7 g/l higher (p ≤ 0.05) than these in control group C and experimental group R and the blood glucose (GLU) in the experimental group R which is with 0.25-0.28 mmol/l lower than determined in control group C and experimental group D.

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The carcass and meat quality characteristics of Japanese quail fed a diet supplemented with powdered Lactuca seriola leaves

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1534 ◽

2021 ◽

Vol 15 ◽

pp. 521-527

Author(s):

Azad Sabow ◽

Nazim Abdulla ◽

Bestoon Ahmad

Keyword(s):

Fatty Acid ◽

Growth Performance ◽

Meat Quality ◽

Polyunsaturated Fatty Acid ◽

Japanese Quail ◽

Dietary Supplementation ◽

Control Group ◽

Omega 3 ◽

Breast Meat ◽

Lactuca Serriola

The present study was aimed to evaluate the effect of dietary supplementation with Lactuca serriola leaves on growth performance, carcass characteristics, meat quality, and its antioxidant stability of Japanese quails. A total of eighty growing quails (1-week old) were distributed into 2 equal groups consisting of 40 birds (5 replicates of 8 birds each). The first group was fed a basal diet without Lactuca serriola leaves (0 g.kg-1 diet) and the second group received diets containing 20 g.kg-1 Lactuca serriola leaves. At age of 6 weeks, quails were slaughtered for meat and carcass examinations. The growth performance for the quails fed with Lactuca serriola leaves diet was similar to that of a control group. Carcass measurements, physical properties, and chemical composition of quail breast meat did not differ between the control and experimental group. However, the inclusion of Lactuca serriola leaves significantly increased the omega-3 polyunsaturated fatty acid content and improved breast meat lipid stability during postmortem refrigerated storage compared to the control diet. In conclusion, dietary supplementation of Lactuca serriola leaves can improve the performance of Japanese quail enrich its meat with an omega-3 polyunsaturated fatty acid, and reduce lipid oxidation during storage.

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