scholarly journals Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period

Antioxidants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 21 ◽  
Author(s):  
Gianfranco Gabai ◽  
Elisabetta De Luca ◽  
Giovanni Miotto ◽  
Gianni Zin ◽  
Annalisa Stefani ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Protein Oxidation ◽  
Protein Concentration ◽  
Low Fertility ◽  
Polymorphonuclear Neutrophil ◽  
Pharmacological Treatments ◽  
Inflammatory Status ◽  
Uterine Health ◽  
Subclinical Endometritis ◽  
The Relationship

High neutrophil (PMN, Polymorphonuclear neutrophil) counts in the endometrium of cows affected by endometritis, suggests the involvement of oxidative stress (OS) among the causes of impaired fertility. Protein oxidation, in particular, advanced oxidation protein products (AOPP), are OS biomarkers linked to PMN activity. To test this hypothesis, the relationship between protein oxidation and uterus health was studied in thirty-eight dairy cows during the puerperium. The animals were found to be cycling, without any signs of disease and pharmacological treatments. PMN count was performed either through a cytobrush or a uterine horn lavage (UHL). Cows were classified into four groups, based on the uterine ultrasonographic characteristics and the PMN percentage in the uterine horns with a higher percentage of high neutrophil horn (HNH). They were classified as: Healthy (H); Subclinical Endometritis (SCE); Grade 1 Endometritis (EM1); and Grade 2 Endometritis (EM2). AOPP and carbonyls were measured in plasma and UHL. UHL samples underwent Western blot analysis to visualize the carbonyl and dityrosine formation. Plasma AOPP were higher (p < 0.05) in EM2. AOPP and carbonyl group concentrations were higher in the HNH samples (p < 0.05). Protein concentration in the UHL was higher in the EM2 (p < 0.05). Carbonyl and dityrosine formation was more intense in EM1 and EM2. Protein oxidation observed in the EM2 suggests the presence of an inflammatory status in the uterus which, if not adequately hindered, could result in low fertility.

scholarly journals Adipokines as biomarkers of postpartum subclinical endometritis in dairy cows

Reproduction ◽  
2020 ◽  
Vol 160 (3) ◽  
pp. 417-430
Author(s):  
Gonçalo Pereira ◽  
Ricardo Bexiga ◽  
João Chagas e Silva ◽  
Elisabete Silva ◽  
Christelle Ramé ◽  
...  
Keyword(s):  
At Risk ◽  
Protein Expression ◽  
Dairy Cows ◽  
Gene Transcription ◽  
Plasma Concentrations ◽  
Inflammatory Status ◽  
Uterine Fluid ◽  
Subclinical Endometritis ◽  
The Relationship ◽  
Cellular Pellet

Adipokines emerged as regulators of metabolism and inflammation in several scenarios. This study evaluated the relationship between adipokines (adiponectin, chemerin and visfatin) and cytological (subclinical) endometritis, by comparing healthy (without), transient (recovered by 45 days postpartum (DPP)) and persistent (until 45 DPP) endometritis cows (n = 49). Cows with persistent endometritis had higher adiponectin concentrations in plasma (at 21 DPP, P < 0.05 and at 45 DPP, P < 0.01) and in uterine fluid (at 45 DPP, P < 0.001), and higher chemerin concentrations in plasma (P < 0.05) and uterine fluid (P < 0.01) at 45 DPP than healthy cows. Cows with persistent endometritis had higher gene transcription in the cellular pellet of uterine fluid and protein expression in the endometrium of these adipokines and their receptors than healthy cows. Adiponectin plasma concentrations allowed to discriminate healthy from persistent endometritis cows, in 87% (21 DPP) and 98% (45 DPP) of cases, and adiponectin and chemerin uterine fluid concentrations at 45 DPP allowed for this discrimination in 100% of cases. Cows with concentrations above the cutoff were a minimum of 3.5 (plasma 21 DPP), 20.4 (plasma 45 DPP), and 33.3 (uterine fluid 45 DPP) times more at risk of evidencing persistent endometritis at 45 DPP than cows with concentrations below the cutoff. Overall, results indicate a relationship between adipokine signalling and the inflammatory status of the postpartum uterus of dairy cows, evidencing that adipokines represent suitable biomarkers of subclinical endometritis, able to predict the risk of persistence of inflammation.

scholarly journals Addition of Clinoptilolite In The Diet Reduces Uterine PMN Leukocytes And Open Days In Multiparous Lactating Dairy Cows Managed In A Mountain Tropical Pasture-Based System

2021 ◽  
Author(s):  
Franklin I Sinchi ◽  
Jenny F. Zuin ◽  
Juan Pablo Garzón ◽  
Gonzalo E. López ◽  
Guido Calle ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Reproductive Performance ◽  
General Linear Model ◽  
Tropical Pasture ◽  
Chi Square ◽  
Lactating Dairy Cows ◽  
Uterine Health ◽  
Pmn Leukocytes ◽  
Chi Square Test ◽  
Subclinical Endometritis

Abstract This study was aimed to assess the effect of adding clinoptilolite in the diet on uterine health and reproductive performance in multiparous lactating dairy cows managed in a tropical pasture-based system above 2500 meters of altitude. Seventy-seven multiparous Holstein crossbred cows were allocated randomly into two groups: clinoptilolite supplemented cows (CLG, n = 42) and non-supplemented cows as control (CG, n = 35). Cows from CLG were supplemented with clinoptilolite from 30 days (50 g/cow/day) before to 60 days after calving (200 g/cow/day). Data were analyzed by general linear model, least squares means and chi-square test of SAS. In CLG cows, percentages of uterine PMN leukocytes (P<0.0001) and proportion of subclinical endometritis (P=0.0187) were lower than in CG. The interval calving to first corpus luteum was shorter (P=0.0759) in CLG than CG, and calving to first service interval was similar between treatments. Cows from CLG became pregnant 35 days earlier than CG cows (P=0.0224). In conclusion, daily addition of clinoptilolite in the diet decreased the proportion of cows with subclinical endometritis and shortened the interval from calving to conception in lactating dairy cows.

scholarly journals Relationship between inflammatory biomarkers and oxidative stress with uterine health in dairy cows with different dry period lengths1

2019 ◽  
Vol 3 (2) ◽  
pp. 607-619
Author(s):  
Novi Mayasari ◽  
Erminio Trevisi ◽  
Annarita Ferrari ◽  
Bas Kemp ◽  
Henk K Parmentier ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dairy Cows ◽  
Negative Energy ◽  
Inflammatory Biomarkers ◽  
Early Lactation ◽  
Dry Period ◽  
Inflammatory Status ◽  
Uterine Health ◽  
Uterine Environment ◽  
And Oxidative Stress

Abstract Earlier studies indicated that the inflammatory status of dairy cows in early lactation could not be fully explained by the negative energy balance (NEB) at that moment. The objective of the present study was to determine relationships between inflammatory biomarkers and oxidative stress with uterine health in dairy cows after different dry period lengths. Holstein–Friesian dairy cows were assigned to one of three dry period lengths (0-, 30-, or 60-d) and one of two early lactation rations (glucogenic or lipogenic ration). Cows were fed either a glucogenic or lipogenic ration from 10-d before the expected calving date. Part of the cows which were planned for a 0-d dry period dried themselves off and were attributed to a new group (0 → 30-d dry period), which resulted in total in four dry period groups. Blood was collected (N = 110 cows) in weeks −3, −2, −1, 1, 2, and 4 relative to calving to determine biomarkers for inflammation, liver function, and oxidative stress. Uterine health status (UHS) was monitored by scoring vaginal discharge (VD) based on a 4-point scoring system (0, 1, 2, or 3) in weeks 2 and 3 after calving. Cows were classified as having a healthy uterine environment (HU, VD score = 0 or 1 in both weeks 2 and 3), nonrecovering uterine environment (NRU, VD score = 2 or 3 in week 3), or a recovering uterine environment (RU, VD score = 2 or 3 in week 2 and VD score= 0 or 1 in week 3). Independent of dry period length, cows with NRU had higher plasma haptoglobin (P = 0.05) and lower paraoxonase levels (P &lt; 0.01) in the first 4 weeks after calving and lower liver functionality index (P &lt; 0.01) compared with cows with HU. Cows with NRU had lower plasma albumin (P = 0.02) and creatinine (P = 0.02) compared with cows with a RU, but not compared with cows with HU. Independent of UHS, cows with a 0 → 30-d dry period had higher bilirubin levels compared with cows with 0-, 30-, or 60-d dry period (P &lt; 0.01). Cows with RU and fed a lipogenic ration had higher levels of albumin in plasma compared with cows with NRU and fed a lipogenic ration (P &lt; 0.01). In conclusion, uterine health was related to biomarkers for inflammation (haptoglobin and albumin) and paraoxonase in dairy cows in early lactation. Cows which were planned for a 0-d dry period, but dried themselves off (0 → 30-d dry period group) had higher bilirubin levels, which was possibly related to a more severe NEB in these cows. Inflammatory biomarkers in dairy cows in early lactation were related to uterine health in this period.

scholarly journals The relationship between endometrial cytology during estrous cycle and cutoff points for the diagnosis of subclinical endometritis in grazing dairy cows

2013 ◽  
Vol 96 (7) ◽  
pp. 4333-4339 ◽  
Author(s):  
L.V. Madoz ◽  
M.J. Giuliodori ◽  
M. Jaureguiberry ◽  
J. Plöntzke ◽  
M. Drillich ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Estrous Cycle ◽  
Cutoff Points ◽  
Grazing Dairy Cows ◽  
Subclinical Endometritis ◽  
The Relationship ◽  
Endometrial Cytology

scholarly journals The relationship between polymorphism of the CXCR1 gene and the risk of endometritis in Holstein dairy cows

2020 ◽  
Vol 90 (6) ◽  
pp. 557-563
Author(s):  
Reza Asadpour ◽  
◽  
Logman Feyzi ◽  
Razi Jafari-Joozani ◽  
Hossain Hamali ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Control Group ◽  
Nucleotide Position ◽  
Single Nucleotide ◽  
Uterine Health ◽  
Pcr Rflp ◽  
History Of ◽  
The Relationship ◽  
Resistant Genotypes ◽  
Holstein Dairy Cows

Endometritis is one of the reproductive diseases that can cause disturbances of postpartum uterine health in cattle. Therefore, identification of resistant genotypes to endometritis is essential. The objective of this study was to evaluate the association between the single nucleotide polymorphism in the interleukin-8 receptor-α (CXCR1) gene and the possibility of endometritis in Holstein dairy cows. For this purpose, blood samples were collected from multiparous dairy cows with a history of clinical endometritis (n = 30), and normal, healthy cows as the control group (n = 10). Deoxyribonucleic acid (DNA) was isolated from a blood sample. To determine genotype, the polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP) was used. The results indicated the presence of a different proportion of polymorphisms (G > C) in the CXCR1 gene in cows with clinical endometritis, compared to the control group. Statistical analyses showed that there is a significant correlation between the incidence of the disease and the CXCR1 genotype in nucleotide position 956. The incidence rate of clinical endometritis was associated with the CXCR1.956 genotype; cows with genotype GC had a higher incidence of clinical endometritis compared with cows with the GG genotype. Overall, the results showed that CXCR1 polymorphism could be a useful marker for identification of resistant genotypes to endometritis in Holstein dairy cows.

The relationship between milk composition and volatile fatty acids in the rumen in cattle

2001 ◽  
Vol 2001 ◽  
pp. 194-194
Author(s):  
T. Yan ◽  
R. E. Agnew
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Fat ◽  
Protein Concentration ◽  
Volatile Fatty Acids ◽  
Milk Composition ◽  
Empirical Relationships ◽  
The Relationship

It is well recognised that the fat and protein concentration in the milk of dairy cows is influenced by volatile fatty acids (VFAs) produced in the rumen. There has however been little information available on models to predict milk composition from rumen VFAs in the literature. The objective of the present study was to develop empirical relationships to predict milk fat and protein concentration using rumen VFA data.

256 Effects of Transition Period Nutrition on Uterine Health

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 136-137
Author(s):  
Felipe Cardoso
Keyword(s):  
Immune Function ◽  
Dairy Cows ◽  
Transition Period ◽  
Neutrophil Infiltration ◽  
High Energy ◽  
Polymorphonuclear Neutrophil ◽  
Blood Concentrations ◽  
Nonesterified Fatty Acids ◽  
Junction Protein ◽  
Uterine Health

Abstract The transition from gestation to lactation, also known as the transition period, is a critical time for dairy cows. This phase is typically defined as 3 wk before parturition through 3 wk after parturition. Peak disease incidence (shortly after parturition) corresponds with the time of greatest negative energy balance (NEB), the peak in blood concentrations of nonesterified fatty acids, and the greatest acceleration of milk yield. Decreased fertility in the face of increasing milk production may be attributable to greater severity of postpartal NEB resulting from inadequate transition management or increased rates of disease. The depth and duration of NEB is highly related to dry matter intake. Formulation and delivery of appropriate diets that limit total energy intake to requirements but also provide proper intakes of all other nutrients (including the most limiting amino acids Met and Lys) before calving can help lessen the extent of NEB after calving. Cows that received controlled-energy diets during the last 3 wk prepartum had shorter days to pregnancy than cows that consumed high-energy diets in this time period. Additionally, a fully acidified prepartum diet formulated with a higher Ca concentration improved reproductive performance and uterine immune function in the postpartum period through the decrease of days to first ovulation, a tendency to decrease service per conception rate, improved glandular morphology, a tendency to increase polymorphonuclear neutrophil infiltration, and a tendency to increase the tight-junction protein occludin. Supplementation of rumen-protected methionine during the transition period resulted in improved uterine immune function through improved glandular morphology, increased neutrophil infiltration number after calving, and discovery of neutrophil extracellular trap formation in bovine endometrial tissue. In conclusion, nutritional strategies during the transition impact uterine health and fertility in dairy cows.

scholarly journals Do Spousal Intimate Relationships Affect Fertility Intentions and Preferences?

2021 ◽  
pp. 0192513X2110160
Author(s):  
Amir Erfani ◽  
Roya Jahanbakhsh
Keyword(s):  
Intimate Relationships ◽  
Mate Selection ◽  
Risk Profile ◽  
Fertility Intentions ◽  
Low Fertility ◽  
Social Forces ◽  
Number Of Children ◽  
Ideal Number ◽  
Using Data ◽  
The Relationship

The fertility influence of spousal intimate relationships is unknown. Drawing on the Giddens’s theory of transformation of intimacy, this study proposed a hypothesis that couples supporting egalitarian intimate relationships, with a greater risk profile attached to the relationship, and having less attachments to the external normative pressures shaping marital relations, are more likely to have low-fertility intentions and preferences. Using data from a self-administered pilot survey ( n = 375 prospective grooms and brides) designed by the authors, and employing multivariate regression models, we found that the lower attachment to external social forces in mate selection was associated with the lower ideal number of children, and those with a greater spousal relational egalitarianism and a higher risk profile attached to their relationships preferred lower number of children and were less likely to intend to have children after marriage. The study sheds new light on the determinants of low fertility.

scholarly journals Reproductive Tract Infections in Dairy Cows: Can Probiotics Curb Down the Incidence Rate?

Dairy ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 40-64
Author(s):  
Eduardo Barahona Rosales ◽  
Burim N. Ametaj
Keyword(s):  
Dairy Cows ◽  
Reproductive Tract ◽  
Negative Impact ◽  
Systemic Circulation ◽  
Contributing Factors ◽  
Reproductive Tract Infections ◽  
Uterine Health ◽  
Lower Fertility ◽  
Tract Infections ◽  
Uterine Diseases

Postpartum uterine diseases are common in dairy cows and are a great concern for the dairy industry as they are associated with various consequences, including lower fertility, lower milk yield, and an overall negative impact on the host health. An infected uterus is a source of bacterial compounds and cytokines that spill into the systemic circulation, spreading inflammation to other organs. In this review article, we discuss a short overview of the anatomy of the reproductive tract of dairy cows and several infectious diseases of the uterus including metritis, endometritis, and pyometra. Additionally, we discuss the microbiome of the reproductive tract in health and during uterine diseases. As well, diagnostic criteria for metritis and endometritis and contributing factors for increased susceptibility to metritis infection are important topics of this review. To better understand how the uterus and reproductive tract respond to bacterial pathogens, a section of this review is dedicated to immunity of the reproductive tract. Both the innate and adaptive immunity systems are also discussed. We conclude the review with a factual discussion about the current treatments of uterine diseases and the new developments in the area of application of probiotics for uterine health. Mechanisms of actions of probiotics are discussed in detail and also some applications to prevent uterine infections in dairy cows are discussed.

scholarly journals Effects of abomasal infusion of essential fatty acids and conjugated linoleic acid on performance and fatty acid, antioxidative, and inflammatory status in dairy cows

2020 ◽  
Vol 103 (1) ◽  
pp. 972-991 ◽  
Author(s):  
S. Haubold ◽  
C. Kröger-Koch ◽  
A. Starke ◽  
A. Tuchscherer ◽  
A. Tröscher ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Essential Fatty Acids ◽  
Inflammatory Status ◽  
Abomasal Infusion
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