scholarly journals Environmentally Relevant Concentrations of Triclosan Induce Cyto-Genotoxicity and Biochemical Alterations in the Hatchlings of Labeo rohita

2021 ◽  
Vol 11 (21) ◽  
pp. 10478
Author(s):  
Sunil Sharma ◽  
Owias Iqbal Dar ◽  
Megha Andotra ◽  
Simran Sharma ◽  
Arvinder Kaur ◽  
...  
Keyword(s):  
Total Bilirubin ◽  
Labeo Rohita ◽  
Tap Water ◽  
Recovery Period ◽  
Tail Length ◽  
Tail Moment ◽  
Biochemical Alterations ◽  
Solvent Control ◽  
Cellular Abnormalities ◽  
Significant Concentration

Xenobiotic Triclosan (TCS) is of great concern because of its existence in a variety of personal, household and healthcare products and continuous discharge in water worldwide. Excessive use of TCS-containing sanitizers and antiseptic products during the COVID-19 pandemic further increased its content in aquatic ecosystems. The present study deals with the cyto-genotoxic effects and biochemical alterations in the hatchlings of Labeo rohita on exposure to environmentally relevant concentrations of TCS. Three-days-old hatchlings were exposed to tap water, acetone (solvent control) and 4 environmentally relevant concentrations (6.3, 12.6, 25.2 and 60 µg/L) of TCS for 14 days and kept for a recovery period of 10 days. The significant concentration-dependent decline in cell viability but increase in micronucleated cells, nucleo-cellular abnormalities (NCAs) and DNA damage parameters like tail length, tail moment, olive tail moment and percent of tail DNA after exposure persisted till the end of recovery period. Glucose, triglycerides, cholesterol, total protein, albumin, total bilirubin, uric acid and urea (except for an increase at 60 µg/L) showed significant (p ≤ 0.05) concentration-dependent decrease after 14 days of exposure. The same trend (except for triglycerides, albumin and total bilirubin) continued till 10 days post exposure. In comparison to control, transaminases (alanine and aspartate aminotransferases) increased (p ≤ 0.05) after exposure as well as the recovery period, while a decline in alkaline phosphatase after exposure was followed by a significant increase during the recovery period. The results show that the environmentally relevant concentrations of TCS cause deleterious effects on the hatchlings of L. rohita.

scholarly journals Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

2021 ◽  
Author(s):  
Sunil Sharma ◽  
Owias Iqbal Dar ◽  
Kirpal Singh ◽  
Sharad Thakur ◽  
Anup Kumar Kesavan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Labeo Rohita ◽  
Recovery Period ◽  
Principal Component ◽  
Tail Length ◽  
Tail Moment ◽  
Risk Quotient ◽  
Biological Responses ◽  
Highly Sensitive ◽  
Genomic Markers

Abstract Triclosan (TCS) used commonly in pharmaceuticals and personal care products has become the most common pollutant in water. Three days old hatchlings of an indegenous fish, Labeo rohita were given 96h exposure to an environmentally relevant (0.06mg/L) and two moderately lethal concentrations (0.067 and 0.097 mg/L) of TCS and kept for 10 days of recovery for recording transcriptomic alterations in antioxidant/detoxification (SOD, GST, CAT, GPx, GR, CYP1a and CYP3a), metabolic (LDH, ALT and AST) and neurological (AchE) genes and DNA damage. The data were subjected to Principal Component Analysis (PCA) for obtaining biomarkers for the toxicity of TCS. Hatchlings were highly sensitive to TCS (96h LC 50 = 0.126mg/L and risk quotient = 40.95), 96h exposure caused significant induction of CYP3a, AChE and ALT but suppression of all other genes. However, expression of all the genes increased significantly (except for a significant decline in ALT) after recovery. Concentration dependent increase was also observed in DNA damage [Tail Length (TL), Tail Moment (TM), Olive Tail Moment (OTM) and Percent Tail DNA (TDNA)] after 96h. The damage declined significantly over 96h values at 0.06 and 0.067 mg/L after recovery, but was still several times more than control. TCS elicited genomic alterations resulted in 5-11% mortality of exposed hatchlings during the recovery period. It is evident that hatchlings of L. rohita are a potential model and PCA shows that OTM, TL, TM, TDNA, SOD and GR (association with PC1 during exposure and recovery) are the biomarkers for the toxicity of TCS.

In vitro toxicological assessment of flumethrin’s effects on MCF-7 breast cancer cells

2021 ◽  
pp. 096032712110227
Author(s):  
S Kara-Ertekin ◽  
S Yazar ◽  
M Erkan
Keyword(s):  
Breast Cancer ◽  
Cancer Cell Line ◽  
Tail Length ◽  
Tail Moment ◽  
High Concentration ◽  
Toxicological Assessment ◽  
Estrogenic Effects ◽  
Pyrethroid Pesticides ◽  
Mcf 7

Pyrethroid pesticides are frequently used for household insect control of insects and in agriculture and livestock. Flumethrin is a pyrethroid that is used against ectoparasites in many animals. The goal of this study was to evaluate the cytotoxic, apoptotic, genotoxic, and estrogenic effects of flumethrin on the mammalian breast cancer cell line (MCF-7). Compared with control groups, a dose-dependent decrease was observed in cell viability at concentrations of 100 µM and higher. The cytotoxic and apoptotic effects detected by LDH assay and AO/EtBr staining increased significantly at a concentration of 1000 µM. The expression of BCL2, which is an anti-apoptotic gene, significantly decreased, whereas BAX, TP53, and P21 expression significantly increased. The results of a comet assay indicated that flumethrin significantly changed tail length, tail % DNA, tail moment, and Olive tail moment in concentrations above 1 and 10 µM. In addition, a 0.1 µM concentration of flumethrin affected ERα receptor mediated cell proliferation and increased transcription of estrogen-responsive pS2 (TFF1) and progesterone receptor (PGR) genes. As a result, flumethrin-induced apoptosis and cytotoxicity at a high concentration, while induced genotoxicity even at lower concentrations. Flumethrin is an endocrine disrupting insecticide with estrogenic effects at very low concentrations.

scholarly journals THE BIOCHEMICAL ALTERATION AND DNA DAMAGE IN RATS (RATTUS RATTUS) AFTER CHRONIC INTRAPERITONEALLY INJECTION TO PURIFIED MICROCYSTIN-LR FROM ANABAENA CIRCINALIS

2017 ◽  
Vol 10 (11) ◽  
pp. 277
Author(s):  
Maher M Khadairi ◽  
Moayed Jy Al-amari ◽  
Ayad Mj Al-mamoori
Keyword(s):  
Dna Damage ◽  
Gel Electrophoresis ◽  
Biochemical Markers ◽  
Chronic Exposure ◽  
High Performance ◽  
Rattus Rattus ◽  
Tail Length ◽  
Oxygen Species ◽  
Tail Moment ◽  
Single Cell Gel Electrophoresis

  Objective: This study determined the effect of purified microcystin-leucine arginine (MC-LR) on biochemical and DNA damage parameters in rats.Methods: Utilization of preparative high-performance liquid chromatography in analysis, purification and collection of MC-LR, then intraperitoneally injection of purified MC-LR to rats. At the end of exposure, animals were sacrificed, and liver cell was isolated to measure the biochemical markers such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) as well as measured malondialdehyde (MDA), reactive oxygen species (ROS) and cytochrome P450 (Cyt P450), and DNA damage markers such as comet length, tail length, and tail moment were measured with the single cell gel electrophoresis also called comet assay.Results: The present results showed significantly increased activities of SOD as well as concentration of MDA, ROS with increasing concentration of MC-LR but the activities of CAT and GSH, as well as Cyt P450, were significantly decreased with increasing MC-LR dose while makers of DNA damage such as comet length, tail length, and tail moment also significantly increased with increasing MC-LR dose.Conclusion: This study demonstrated that chronic exposure to MC-LR toxin can induce alteration of biochemical and DNA damage markers.

scholarly journals Fertilization-Induced Changes in Growth Parameters and Antioxidant Activity of Medicinal Plants Used in Traditional Arab Medicine

2005 ◽  
Vol 2 (4) ◽  
pp. 549-556 ◽  
Author(s):  
Hassan Azaizeh ◽  
Predrag Ljubuncic ◽  
Irina Portnaya ◽  
Omar Said ◽  
Uri Cogan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Therapeutic Potential ◽  
Growth Parameters ◽  
Tap Water ◽  
Water Soluble ◽  
Wild Plants ◽  
Hoagland Solution ◽  
Β Carotene ◽  
Significant Concentration

In response to increased popularity and greater demand for medicinal plants, a number of conservation groups are recommending that wild medicinal plants be brought into cultivation systems. We collected four medicinal herbsCichorium pumilum, Eryngium creticum,Pistacia palaestinaandTeucrium poliumused in traditional Arab medicine for greenhouse cultivation to assess the effects of different fertilization regimes on their growth and antioxidant activity. Wild seedlings were collected and fertilized with either 100% Hoagland solution, 50% Hoagland solution, 20% Hoagland solution or irrigated with tap water. Plant height was measured and the number of green leaves and branches counted weekly. Thereafter, the aboveground parts of plants were harvested for preparing a water-soluble powder extracts of which antioxidant activity was measured by their ability to suppress the oxidation of β-carotene. Of the fertilization regimes, we found either 20 or 50% Hoagland solution produced the most consistent response of the plant growth parameters. All powders prepared from the four wild growing plants inhibited oxidation of β-carotene. Increasing the amount of fertilizer caused a significant concentration-dependent increase in antioxidant activity of the cultivatedT. poliumcompared with the wild type. In contrast, increasing the amount of fertilizer caused a significant concentration-dependent reduction in the antioxidant activity of powders prepared from the cultivatedE. creticumwhen compared with wild plants. Our results showed that cultivation success should not rely solely on parameters of growth but should incorporate assessment related to indices of therapeutic potential.

Thermal stress causes nuclear and cellular abnormalities of peripheral erythrocytes in Indian major carp, rohu Labeo rohita

2019 ◽  
Vol 86 ◽  
pp. 102450 ◽  
Author(s):  
Mohammad Ashaf-Ud-Doulah ◽  
Md. Shahjahan ◽  
S M Majharul Islam ◽  
Md. Al-Emran ◽  
Mohammad Shadiqur Rahman ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Labeo Rohita ◽  
Indian Major Carp ◽  
Cellular Abnormalities

Sesamol Supplementation Mitigates Isoproterenol-Induced Cardiac Toxicity in Rats by Stabilizing Cardiac Mitochondrial and Lysosomal Enzymes

2021 ◽  
Vol 16 (12) ◽  
pp. 1934578X2110579
Author(s):  
Lakshmanan Vennila ◽  
Kodukkur Viswanathan Pugalendi ◽  
Thangaiyan Radhiga
Keyword(s):  
Lysosomal Enzymes ◽  
Nadh Dehydrogenase ◽  
Cardiac Toxicity ◽  
Tail Length ◽  
Tca Cycle ◽  
Tricarboxylic Acid ◽  
Tail Moment ◽  
Tca Cycle Enzymes ◽  
Wistar Strain ◽  
Heart Homogenate

The current investigation was intended to evaluate the antimyocardial ischemic effects of sesamol on lactate dehydrogenase (LDH) isoenzymes, DNA damage, and mitochondrial and lysosomal enzyme activities in isoproterenol (ISO)-induced myocardial infarction (MI) in male albino Wistar strain rats. Rats that received ISO (85 mg/kg body weight (B.W) subcutaneously) for the first 2 consecutive days showed significant reduction in the activities of tricarboxylic acid (TCA) cycle enzymes (isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, malate dehydrogenase, and succinate dehydrogenase) and respiratory chain enzymes (cytochrome c oxidase and nicotinamide adenine dinucleotide hydrogen (NADH) dehydrogenase) in the heart mitochondria. The activities of the lysosomal enzymes (α-and β-glucosidases, α and β-galactosidases, β-glucuronidase and β-N-acetyl glucosaminidase and cathepsin-B and cathepsin-D) were increased significantly in the heart homogenate of ISO-induced MI rats. ISO injection also increased the % of tail DNA, tail length, and tail moment and decreased the % of head DNA. Pretreatment with sesamol (50 mg/kg B.W) every day for a period of 9 days prevented the above abnormalities induced by ISO. In conclusion, it can be inferred that administration of sesamol has a potent beneficial role against ISO-induced damage to the mitochondria, lysosomes, and DNA, thereby preventing MI.

scholarly journals High sodium intake during postnatal phases induces an increase in arterial blood pressure in adult rats

2014 ◽  
Vol 112 (12) ◽  
pp. 1923-1932 ◽  
Author(s):  
M. C. S. Moreira ◽  
E. F. da Silva ◽  
L. L. Silveira ◽  
Y. B. de Paiva ◽  
C. H. de Castro ◽  
...  
Keyword(s):  
Sodium Intake ◽  
Tap Water ◽  
Recovery Period ◽  
Control Group ◽  
Adult Rats ◽  
High Sodium ◽  
Arterial Blood ◽  
Hypertension Development ◽  
Lower Magnitude ◽  
Experimental Group

Epigenetic studies suggest that diseases that develop in adulthood are related to certain conditions to which the individual is exposed during the initial stages of life. Experimental evidence has demonstrated that offspring born to mothers maintained on high-Na diets during pregnancy have higher mean arterial pressure (MAP) in adulthood. Although these studies have demonstrated the importance of prenatal phases to hypertension development, no evidence regarding the role of high Na intake during postnatal phases in the development of this pathology has been reported. Therefore, in the present study, the effects of Na overload during childhood on induced water and Na intakes and on cardiovascular parameters in adulthood were evaluated. Experiments were carried out in two groups of 21-d-old rats: experimental group, maintained on hypertonic saline (0·3 m-NaCl) solution and food for 60 d, and control group, maintained on tap water and food. Later, both groups were given water and food for 15 d (recovery period). After the recovery period, chronic cannulation of the right femoral artery was performed in unanaesthetised rats to record baseline MAP and heart rate (HR). The experimental group was found to have increased basal MAP (98·6 (sem 2·6) v. 118·3 (sem 2·7) mmHg, P< 0·05) and HR (365·4 (sem 12·2) v. 398·2 (sem 7·5) beats per min, P< 0·05). There was a decrease in the baroreflex index in the experimental group when compared with that in the control group. A water and Na intake test was performed using furosemide. Na depletion was found to induce an increase in Na intake in both the control and experimental groups (12·1 (sem 0·6) ml and 7·8 (sem 1·1), respectively, P< 0·05); however, this increase was of lower magnitude in the experimental group. These results demonstrate that postnatal Na overload alters behavioural and cardiovascular regulation in adulthood.

Genetic Toxicity of Berberine on Mouse Heart

2014 ◽  
Vol 884-885 ◽  
pp. 634-637
Author(s):  
Cheng Bin Xu ◽  
Lu Wang ◽  
Xiu Juan Hui ◽  
Xi Ping Ma ◽  
Xin Bi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Clinical Medicine ◽  
Tail Length ◽  
Dose Effect ◽  
Mouse Heart ◽  
Heart Cells ◽  
Tail Moment ◽  
Biochemical Responses ◽  
Common Drug ◽  
Change Tendency

Berberine, a common drug, which is widely used in clinical medicine as inflammatory medicines, is rarely studied about its affection on non-target organisms in recent researches. To evaluate the eco-toxicity of berberine in mouse heart cells, biochemical responses including oxidative damage and changes in the activities of antioxidative enzymes catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) induced by berberine were investigated in mouse heart cells. 7.5, 15, 30, 60 and 120 mgkg-1 berberine was added to mouse heart cells respectively. Compared to the controls, the damage, tail DNA content (%) , tail length and tail moment went up respectively in dose-effect manner following the rising berberine; As the content of berberine increased, the activities of SOD, CAT and POD were gradually decreased and the concentration of MDA was gradually increased. Compared to the controls, the change tendency showed significant differences (p<0.05 or p<0.01). In conclusion, berberine induces oxidative stress and DNA damage to heart cells, which may be the significant mechanisms for its toxicity to mice.

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