scholarly journals Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties

Ceramics ◽  
2021 ◽  
Vol 4 (4) ◽  
pp. 628-651
Author(s):  
Konstantina Kazeli ◽  
Ioannis Tsamesidis ◽  
Anna Theocharidou ◽  
Lamprini Malletzidou ◽  
Jonathan Rhoades ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Treatment ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Biological Properties ◽  
Apatite Formation ◽  
Bacterial Strains ◽  
Synthesized Materials ◽  
And Oxidative Stress

Glass-ceramic nanopowder with a composition of 55SiO2-35CaO-10MgO (mol %) was synthesized by the sol–gel method and was heat treated at three temperatures (T1 = 835 °C, T2 = 1000 °C, T3 = 1100 °C) in order to obtain different materials (C1, C2, C3, respectively) varying in crystal structure. Bioactivity and oxidative stress were evaluated in simulated body fluid (SBF) for various time periods (up to 10 days). The structure of the synthesized materials and their apatite-forming ability were investigated by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy and Energy Dispersive Spectroscopy (SEM/EDS). The antibacterial properties of the synthesized materials were evaluated against three Gram-positive and four Gram-negative bacterial strains and their biocompatibility was verified on a primary cell line of human gingival fibroblasts (HGFs) by the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay. The crystallization of the materials was increased by sintering temperature. Heat treatment did not inhibit the bioactive behavior of the materials as apatite formation started after 3 days in SBF. C2, C3 showed some indications of apatite forming even from the first day. Regarding cell viability, a variety of biological behaviors, concerning both dose and time points, was observed between the positive control and the tested materials by both the MTT assay and oxidative stress analysis. In conclusion, the nanobioceramic materials of this study possess a multitude of attractive physicochemical and biological properties that make them suitable candidates for bone regeneration applications, fillers in nanocomposite scaffolds, or as grafts in bone cavities and periodontal lesions.

scholarly journals Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite

2021 ◽  
Vol 22 (8) ◽  
pp. 4246
Author(s):  
Muhammad Maqbool ◽  
Qaisar Nawaz ◽  
Muhammad Atiq Ur Atiq Ur Rehman ◽  
Mark Cresswell ◽  
Phil Jackson ◽  
...  
Keyword(s):  
Negative Impact ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Ion Concentration ◽  
Charge Composition ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Molar Ratios ◽  
Human Osteosarcoma Cells

In this study, as a measure to enhance the antimicrobial activity of biomaterials, the selenium ions have been substituted into hydroxyapatite (HA) at different concentration levels. To balance the potential cytotoxic effects of selenite ions (SeO32−) in HA, strontium (Sr2+) was co-substituted at the same concentration. Selenium and strontium-substituted hydroxyapatites (Se-Sr-HA) at equal molar ratios of x Se/(Se + P) and x Sr/(Sr + Ca) at (x = 0, 0.01, 0.03, 0.05, 0.1, and 0.2) were synthesized via the wet precipitation route and sintered at 900 °C. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and cell viability were studied. X-ray diffraction verified the phase purity and confirmed the substitution of selenium and strontium ions. Acellular in vitro bioactivity tests revealed that Se-Sr-HA was highly bioactive compared to pure HA. Se-Sr-HA samples showed excellent antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus carnosus) bacterial strains. In vitro cell–material interaction, using human osteosarcoma cells MG-63 studied by WST-8 assay, showed that Se-HA has a cytotoxic effect; however, the co-substitution of strontium in Se-HA offsets the negative impact of selenium and enhanced the biological properties of HA. Hence, the prepared samples are a suitable choice for antibacterial coatings and bone filler applications.

scholarly journals Biological Properties of a Citral-Enriched Fraction of Citrus limon Essential Oil

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1290
Author(s):  
Marzia Pucci ◽  
Stefania Raimondo ◽  
Chiara Zichittella ◽  
Vincenza Tinnirello ◽  
Valeria Corleone ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Essential Oil ◽  
Biological Activities ◽  
Protective Action ◽  
Biological Properties ◽  
Citrus Limon ◽  
Tnf Α ◽  
Pre Treatment ◽  
Flavoring Agent ◽  
And Oxidative Stress

Lemon essential oil (LEO) is a well-known flavoring agent with versatile biological activities. In the present study, we have isolated and characterized four citral-enriched fractions of winter LEO. We reported that in murine and human macrophages the pre-treatment with a mix of these fractions (Cfr-LEO) reduces the expression of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 induced by LPS. In addition, Cfr-LEO counteracts LPS-induced oxidative stress, as shown by the increase in the GSH/GSSG ratio in comparison to cells treated with LPS alone. Overall, the results reported here encourage the application of EO fractions, enriched in citral, in the nutraceutical industry, not only for its organoleptic properties but also for its protective action against inflammation and oxidative stress.

scholarly journals Preparation of Long-Term Antibacterial SiO2-Cinnamaldehyde Microcapsule via Sol-Gel Approach as a Functional Additive for PBAT Film

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 897
Author(s):  
Yangfan Xu ◽  
Chongxing Huang ◽  
Xiujie Dang ◽  
Muhammad Rafiullah Khan ◽  
Haohe Huang ◽  
...  
Keyword(s):  
Sustained Release ◽  
Composite Film ◽  
Transmission Rate ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Light Transmittance ◽  
Release Time ◽  
Order Kinetic ◽  
Release Process

The mesoporous silica wall materials can achieve controlled load and sustained-release of active agents. An antimicrobial nanoscale silica microcapsule containing cinnamaldehyde (CA) was prepared by the sol-gel method and applied in poly (butyleneadipate-co-terephthalate) (PBAT) film. The surface morphology, physical and chemical properties, and antibacterial properties of microcapsules and films were studied. The effects of different temperatures and humidities on the release behavior of microcapsules were also evaluated. Results showed that CA was successfully encapsulated in silica microcapsule which had a diameter of 450–700 nm. The antibacterial CA agent had a long-lasting release time under lower temperature and relative humidity (RH) environment. At low temperature (4 °C), the microcapsules released CA 32.35% in the first 18 h, and then slowly released to 56.08% in 216 h; however, the microcapsules released more than 70% in 18 h at 40 °C. At low humidity (50%RH), the release rates of microcapsules at the 18th h and 9th d were 43.04% and 78.01%, respectively, while it reached to equilibrium state at 72 h under 90% RH. The sustained release process of CA in SiO2-CA microcapsules follows a first-order kinetic model. Physicochemical properties of PBAT films loaded with different amounts of microcapsules were also characterized. Results showed that the tensile strength and water vapor transmission rate (WVTR) of the composite film containing 2.5% microcapsules were increased by 26.98% and 14.61%, respectively, compared to the raw film, while the light transmittance was slightly reduced. The crystallinity of the film was improved and can be kept stable up to 384.1 °C. Furthermore, microcapsules and composite film both exhibited distinctive antibacterial effect on Escherichia coli and Listeria monocytogenes. Therefore, SiO2-CA microcapsules and composite films could be a promising material for the active packaging.

scholarly journals Nephrotoxicity Evaluation of Indium Phosphide Quantum Dots with Different Surface Modifications in BALB/c Mice

2020 ◽  
Vol 21 (19) ◽  
pp. 7137
Author(s):  
Li Li ◽  
Tingting Chen ◽  
Zhiwen Yang ◽  
Yajing Chen ◽  
Dongmeng Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
Biomedical Applications ◽  
Renal Involvement ◽  
Chemical Properties ◽  
Surface Chemical ◽  
Biological Fate ◽  
And Oxidative Stress ◽  
Observation Period

InP QDs have shown a great potential as cadmium-free QDs alternatives in biomedical applications. It is essential to understand the biological fate and toxicity of InP QDs. In this study, we investigated the in vivo renal toxicity of InP/ZnS QDs terminated with different functional groups—hydroxyl (hQDs), amino (aQDs) and carboxyl (cQDs). After a single intravenous injection into BALB/c mice, blood biochemistry, QDs distribution, histopathology, inflammatory response, oxidative stress and apoptosis genes were evaluated at different predetermined times. The results showed fluorescent signals from QDs could be detected in kidneys during the observation period. No obvious changes were observed in histopathological detection or biochemistry parameters. Inflammatory response and oxidative stress were found in the renal tissues of mice exposed to the three kinds of QDs. A significant increase of KIM-1 expression was observed in hQDs and aQDs groups, suggesting hQDs and aQDs could cause renal involvement. Apoptosis-related genes (Bax, Caspase 3, 7 and 9) were up-regulated in hQDs and aQDs groups. The above results suggested InP/ZnS QDs with different surface chemical properties would cause different biological behaviors and molecular actions in vivo. The surface chemical properties of QDs should be fully considered in the design of InP/ZnS QDs for biomedical applications.

Ag/CdS Nanocomposite: An Efficient Recyclable Catalyst for the Synthesis of Novel 8-Aryl-8H-[1,3]dioxolo[4,5-g]chromene-6-carboxylic Acids under Mild Reaction Conditions

2018 ◽  
Vol 21 (5) ◽  
pp. 323-328 ◽  
Author(s):  
Shahrzad Abdolmohammadi ◽  
Seyed Reza Rasouli Nasrabadi ◽  
Ahmad Seif ◽  
Narges Elmi Fard
Keyword(s):  
Carboxylic Acids ◽  
Condensation Reaction ◽  
Sol Gel ◽  
Aqueous Media ◽  
Chemical Properties ◽  
Catalytic Amount ◽  
13C Nmr Spectroscopy ◽  
Biological Properties ◽  
Recyclable Catalyst ◽  
Reaction Conditions

Aim and Objective: Chromene derivatives are privileged heterocyclic systems that exhibit various types of biological properties such as antioxidant, anticancer, antimicrobial, hypotensive, and local anesthetic. Cadmium sulfide nanoparticles (CdS NPs) as an efficient heterogeneous catalyst is used in various organic transformations because of its certain unique and unusual physico-chemical properties. The effectiveness of catalytic activity of CdS NPs can be improved due to the combined effect of Ag particles. Results: Ag/CdS nanocomposite is a readily available, recyclable, and non-toxic catalyst used for the highly efficient synthesis of novel 8-aryl-8H-[1,3]dioxolo[4,5-g]chromrne-6-carboxylic acids. This reaction is conveniently performed under mild reaction conditions. All synthesized compounds were well characterized by their satisfactory elemental analyses, IR, 1H and 13C NMR spectroscopy. The synthesized catalyst was fully characterized by XRD, SEM, and EDX techniques. Materials and Methods: The present methodology focuses on the condensation reaction of arylmethylidenepyruvic acids with 3,4-methylenedioxyphenol, using a catalytic amount of Ag/CdS nanocomposite (15 mol%) in aqueous media at room temperature to afford 8-aryl-8H-[1,3]dioxolo [4,5-g]chromrne-6-carboxylic acids in high yields (90-97%) within short reaction times (2-4 h). The Ag/CdS nanocomposite was also prepared by an ultrasonic-assisted sol-gel method. Conclusion: In conclusion, we have successfully synthesized novel 8-aryl-8H-[1,3]dioxolo[4,5- g]chromrne-6-carboxylic acid derivatives by the Ag/CdS nanocomposite catalyzed cyclocondensation reaction of arylmethylidenepyruvic acids with 3,4-methylenedioxyphenol under mild reaction conditions. Environmentally benign procedure, high to excellent yields of products, simplicity of operation, and use of readily available and recyclable catalyst are the advantages of this new practical reaction.

Heat Treatment Effect on Biological Behavior of Polyetheretherketone Composites

2022 ◽  
Vol 54 ◽  
pp. 119-128
Author(s):  
Alaa A. Mohammed ◽  
Jawad K. Oleiwi
Keyword(s):  
Heat Treatment ◽  
Simulated Body Fluid ◽  
Mtt Assay ◽  
Body Fluid ◽  
Antibacterial Properties ◽  
Biological Behavior ◽  
Apatite Formation ◽  
Crystalline Polymers ◽  
Density Methods

Polyetheretherketone is a semi-crystalline thermoplastic polymer, that so with heat treatments, it is possible to get different properties which are very important for the material performance. Heat treatment is a broadly utilized to develop the semi-crystalline polymers properties. In the present investigation, annealing of polyetheretherketone (PEEK) was carried out at temperatures above its glass transition temperature (Tg) to study its effects upon the biological conduct of the control and PEEK ternary composites. The bioactivity of the specimens was evaluated by investigating the apatite formation after immersion for different periods in a simulated body fluid (SBF). The biocompatibility of specimens was assessed by MTT assay. Additionally, the antibacterial property of the specimens versus S. aureus was observed with the optical density methods. The results manifested that the formation of hydroxyapatite was obviously observed on specimens after immersion for (7 and 14 days) in the simulated body fluid (SBF). Otherwise, the results of MTT assay recorded the PEEK specimens that excited the activity of fibroblasts, and therefore a high cytocompatibility was noticed and the specimens revealed antibacterial properties against S. aureus. So, the results of the bioactivity, biocompatibility and antibacterial tests in vitro demonstrated that the heat treatment enhanced biological behavior.

Structure and Antibacterial Properties of Ag-Doped TiO2 Porous Materials

2007 ◽  
Vol 330-332 ◽  
pp. 995-998 ◽  
Author(s):  
Hai Rong Liu ◽  
Yi Lin ◽  
Rei Fang Ye ◽  
Li Song ◽  
Qi Chen
Keyword(s):  
Heat Treatment ◽  
Pore Structure ◽  
Antibacterial Property ◽  
Anatase Phase ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Doped Tio2 ◽  
Visible Spectra ◽  
Release Speed

Antibacterial Ag-doped TiO2 porous monolithics were firstly prepared by hybridization of polyethylene glycol, Ti(OC4H9)4 and AgNO3 via sol-gel method following by heat-treatment to remove the organic components. Thermogravimeter−differential thermal analysis, pore structure, infrared spectra, ultraviolet−visible spectra, release speed of silver ions into 30°C water and antibacterial properties of Ag-doped TiO2 samples made at different temperature were studied. The results showed that anatase phase and uniform pore structure can be formed after heated at 500°C. Ag+ ions from the samples heated at 500°C were stably released into water at 30°C up to 14 days. The material treated at 500°C has the best antibacterial property and can restrain Escherichia coli effectively.

scholarly journals Sol–Gel Synthesis of Silica-Based Materials with Different Percentages of PEG or PCL and High Chlorogenic Acid Content

Materials ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 155 ◽  
Author(s):  
Elisabetta Tranquillo ◽  
Federico Barrino ◽  
Giovanni Dal Poggetto ◽  
Ignazio Blanco
Keyword(s):  
Chlorogenic Acid ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Silica Matrix ◽  
Implant Failure ◽  
Bacterial Strains ◽  
Drug Molecules ◽  
Infection And Inflammation ◽  
Sol Gel Synthesis

Implanted biomedical devices can induce adverse responses in the human body, which can cause failure of the implant—referred to as implant failure. Early implant failure is induced numerous factors, most importantly, infection and inflammation. Natural products are, today, one of the main sources of new drug molecules due to the development of pathogenic bacterial strains that possess resistance to more antibiotics used currently in various diseases. The aim of this work is the sol–gel synthesis of antibacterial biomedical implants. In the silica matrix, different percentages (6, 12, 24, 50 wt %) of polyethylene glycol (PEG) or poly(ε-caprolactone) (PCL) were embedded. Subsequently, the ethanol solutions with high amounts of chlorogenic acid (CGA 20 wt %) were slowly added to SiO2/PEG and SiO2/PCL sol. The interactions among different organic and inorganic phases in the hybrid materials was studied by Fourier transform infrared (FTIR) spectroscopy. Furthermore, the materials were soaked in simulated body fluid (SBF) for 21 days and the formation of a hydroxyapatite layer on their surface was evaluated by FTIR and XRD analysis. Finally, Escherichia coli and Pseudomonas aeruginosa were incubated with several hybrids, and the diameter of zone of inhibition was observed to assessment the potential antibacterial properties of the hybrids.

scholarly journals Influence of the Heat Treatment on the Particles Size and on the Crystalline Phase of TiO2 Synthesized by the Sol-Gel Method

Materials ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 2364 ◽  
Author(s):  
Michelina Catauro ◽  
Elisabetta Tranquillo ◽  
Giovanni Dal Poggetto ◽  
Mauro Pasquali ◽  
Alessandro Dell’Era ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Tio2 Nanoparticles ◽  
Sol Gel ◽  
Biological Properties ◽  
Thermal Treatments ◽  
Crystalline Phases ◽  
Gel Method ◽  
Sol Gel Method ◽  
Different Content ◽  
Ideal Technique

Titanium biomaterials’ response has been recognized to be affected by particles size, crystal structure, and surface properties. Chemical and structural properties of these nanoparticle materials are important, but their size is the key aspect. The aim of this study is the synthesis of TiO2 nanoparticles by the sol-gel method, which is an ideal technique to prepare nanomaterials at low temperature. The heat treatment can affect the structure of the final product and consequently its biological properties. For this reason, the chemical structure of the TiO2 nanoparticles synthesized was investigated after each heat treatment, in order to evaluate the presence of different phases formed among the nanoparticles. FTIR spectroscopy and XRD have been used to evaluate the different structures. The results of these analyses suggest that an increase of the calcination temperature induces the formation of mixed-crystalline-phases with different content of anatase and rutile phases. The results obtained by SEM measurements suggest that an increase in the particles size accompanied by a noticeable aggregation of TiO2 nanoparticles is due to high temperatures achieved during the thermal treatments and confirmed the presence of different content of the two crystalline phases of titanium dioxide.

scholarly journals Enhancing Mechanical Properties and Biological Performances of Injectable Bioactive Glass by Gelatin and Chitosan for Bone Small Defect Repair

Biomedicines ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 616
Author(s):  
Mehri Sohrabi ◽  
Bijan Eftekhari Yekta ◽  
Hamidreza Rezaie ◽  
Mohammad Reza Naimi-Jamal ◽  
Ajay Kumar ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Antibacterial Properties ◽  
Human Mesenchymal Stem Cells ◽  
Biological Properties ◽  
Mechanical Resistance ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Small Defect ◽  
Genes Expression ◽  
Defect Repair

Bioactive glass (BG) represents a promising biomaterial for bone healing; here injectable BG pastes biological properties were improved by the addition of gelatin or chitosan, as well as mechanical resistance was enhanced by adding 10 or 20 wt% 3-Glycidyloxypropyl trimethoxysilane (GPTMS) cross-linker. Composite pastes exhibited bioactivity as apatite formation was observed by Scanning Electron Microscopy (SEM) and X-Ray Diffraction (XRD) after 14 days immersion in simulated body fluid (SBF); moreover, polymers did not enhance degradability as weight loss was >10% after 30 days in physiological conditions. BG-gelatin-20 wt% GPTMS composites demonstrated the highest compressive strength (4.8 ± 0.5 MPa) in comparison with the bulk control paste made of 100% BG in water (1.9 ± 0.1 MPa). Cytocompatibility was demonstrated towards human mesenchymal stem cells (hMSC), osteoblasts progenitors, and endothelial cells. The presence of 20 wt% GPTMS conferred antibacterial properties thus inhibiting the joint pathogens Staphylococcus aureus and Staphylococcus epidermidis infection. Finally, hMSC osteogenesis was successfully supported in a 3D model as demonstrated by alkaline phosphatase release and osteogenic genes expression.

Sign in / Sign up

Export Citation Format

Share Document