scholarly journals Value-Added Products from Ethanol Fermentation—A Review

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 267
Author(s):  
Timothy J. Tse ◽  
Daniel J. Wiens ◽  
Farley Chicilo ◽  
Sarah K. Purdy ◽  
Martin J. T. Reaney
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Animal Feed ◽  
Value Added ◽  
Water Soluble ◽  
Added Value ◽  
Yeast Fermentation ◽  
Protein Concentrates ◽  
Fusel Alcohols ◽  
Valuable Compounds

Global demand for renewable and sustainable energy is increasing, and one of the most common biofuels is ethanol. Most ethanol is produced by Saccharomyces cerevisiae (yeast) fermentation of either crops rich in sucrose (e.g., sugar cane and sugar beet) or starch-rich crops (e.g., corn and starchy grains). Ethanol produced from these sources is termed a first-generation biofuel. Yeast fermentation can yield a range of additional valuable co-products that accumulate during primary fermentation (e.g., protein concentrates, water soluble metabolites, fusel alcohols, and industrial enzymes). Distillers’ solubles is a liquid co-product that can be used in animal feed or as a resource for recovery of valuable materials. In some processes it is preferred that this fraction is modified by a second fermentation with another fermentation organism (e.g., lactic acid bacteria). Such two stage fermentations can produce valuable compounds, such as 1,3-propanediol, organic acids, and bacteriocins. The use of lactic acid bacteria can also lead to the aggregation of stillage proteins and enable protein aggregation into concentrates. Once concentrated, the protein has utility as a high-protein feed ingredient. After separation of protein concentrates the remaining solution is a potential source of several known small molecules. The purpose of this review is to provide policy makers, bioethanol producers, and researchers insight into additional added-value products that can be recovered from ethanol beers. Novel products may be isolated during or after distillation. The ability to isolate and purify these compounds can provide substantial additional revenue for biofuel manufacturers through the development of marketable co-products.

scholarly journals Conversion of Whey into Value-Added Products through Fermentation and Membrane Fractionation

Water ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1623
Author(s):  
Alejandro Caballero ◽  
Pablo Caballero ◽  
Federico León ◽  
Bruno Rodríguez-Morgado ◽  
Luis Martín ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Protein Hydrolysate ◽  
Value Added ◽  
Membrane System ◽  
Added Value ◽  
Transport Costs ◽  
Ammonium Lactate ◽  
Fermented Product ◽  
Animal Feeding

The cheese whey (95% composed of water) is an effluent produced in the cheese industry, of which more than 1.5 million tons are generated in Spain, constituting a serious environmental problem. The process starts with a new fermentative/enzymatic technology that totally converts whey, mainly composed by lactose, proteins, and salts, into a fermented product with higher added value. This new product is mainly composed by lactic acid bacteria biomass, ammonium lactate, and a protein hydrolysate. To separate valuable fractions, this fermented product is processed by a two-stage membrane system, which is a very innovative process in this type of fermented product. The first stage consists of ultrafiltration to separate all suspended solids. As a result of this stage, a product mainly constituted by lactic acid bacteria that have both agronomic applications, mainly as a biocontrol and biofertilizer/bio-stimulant, and applications in animal feeding as a probiotic, is obtained. The second stage consists of reverse osmosis used to concentrate the ultrafiltered permeate obtained earlier, leading to a microbiologically stable product and reducing transport costs. The concentrate is mainly composed of ammonium lactate and a protein hydrolysate, constituted by peptides and free amino acids, which has application both in agriculture as a bio-stimulant and in animal feeding, and the permeate is water, reusable in other industrial processes. This work demonstrates the technical feasibility of this valorization process to achieve the objective of “Waste 0” from a problematic by-product, while obtaining products with commercial utility.

scholarly journals Microbial Community and Fermentation Characteristics of Native Grass Prepared Without or With Isolated Lactic Acid Bacteria on the Mongolian Plateau

2021 ◽  
Vol 12 ◽  
Author(s):  
Sihan You ◽  
Shuai Du ◽  
Gentu Ge ◽  
Tao Wan ◽  
Yushan Jia
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacterial Community ◽  
Relative Abundance ◽  
Principal Coordinate Analysis ◽  
Water Soluble ◽  
Dry Matter Content ◽  
Mongolian Plateau ◽  
Native Grass ◽  
Grass Silage

This study aimed to isolate and identify lactic acid bacteria (LAB) from the native grass and naturally fermented silage from the Mongolian Plateau. The effect of selected strains on bacterial community and quality of native grass silage was also studied. Strains XM2, 265, and 842 could grow normally at 15°C–30°C, pH 4.0–8.0, and NaCl 3 and 6.5%; they were identified as Lactiplantibacillus plantarum subsp. plantarum, Pediococcus acidilactici, and Latilactobacillus graminis, by sequencing 16S rRNA, respectively. The three strains (XM2, 265, and 842) and one commercial additive (L) were used as inoculants and singularly added to the native grass. Compared to the control, the dry matter content was significantly (p < 0.05) lower in L and XM2 groups. The water-soluble carbohydrate content was significantly (p < 0.05) higher in control than in other groups. Compared with the control, the crude protein and ammonia nitrogen contents were significantly (p < 0.05) higher and lower in the LAB-treated groups, and the acid and detergent fiber contents were significantly (p < 0.05) reduced in the L and XM2 groups than those in other groups. There was a significant (p < 0.05) difference in the pH value, lactic acid content, and lactic acid-to-acetic acid ratio in L and XM2 groups than in other groups. Compared with the control, the number of LAB was significantly (p < 0.05) higher in LAB-treated silages, whereas no significant (p > 0.05) differences were observed in yeast and aerobic bacteria in all groups. Compared to the control, the Shannon index was significantly (p < 0.05) reduced. Simpson and Chao1 were significantly (p < 0.05) increased. Principal coordinate analysis based on the unweighted UniFrac distance showed clear separation of the bacterial community in fresh materials and LAB-treated silages. Besides, compared to the control, the principal coordinate analysis of LAB-treated silages was also separate. After 30 days of fermentation, the relative abundance of Firmicutes increased and was the primary phylum in all silages. Compared with the control, the abundance of Firmicutes and Proteobacteriawas significantly (p < 0.05) higher and lower in L and XM2 groups. In contrast, no significant differences were observed among control, 265, and 842 groups. At the genus level, the relative abundance of Lactobacillus, Enterobacter, Pediococcus, and Weissella was increased and dominated the native grass fermentation. Compared with the control, the abundance of Lactobacillus was significantly (p < 0.05) higher in L, XM2, and 842 groups, while no significant (p > 0.05) differences were observed between the control and 265 groups. The abundance of Pediococcus was higher than that in other groups. Consequently, the results demonstrated that LAB significantly influenced silage fermentation by reconstructing microbiota, and Lactobacillus was the dominant genus in the native grass silages. Furthermore, the results showed that strain XM2 could effectively improve the silage quality, and it is considered a potential starter for the native grass silage.

LACTIC ACID BACTERIA IN FOOD AND HEALTH: A REVIEW WITH SPECIAL REFERENCE TO ENTEROPATHOGENIC ESCHERICHIA COLI AS WELL AS CERTAIN ENTERIC DISEASES AND THEIR TREATMENT WITH ANTIBIOTICS AND LACTOBACILLI1

1972 ◽  
Vol 35 (12) ◽  
pp. 691-702 ◽  
Author(s):  
W. E. Sannine ◽  
K. S. Muralidhara ◽  
P. R. Elliker ◽  
D. C. England
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Animal Feed ◽  
Large Population ◽  
Intestinal Bacteria ◽  
Transfer Factors ◽  
Enteric Diseases ◽  
Human Vagina ◽  
Vaginal Disease

Recent literature concerning enteropathogenesis and drug resistance transfer factors in Escherichia coli are summarized as well as related papers concerning the use of antibiotics in animal feed. E. coli infection in swine (colibacillosis) also is considered, especially citations indicating the similarity between the disease in man and animals. The role of intestinal bacteria in human health is reviewed, emphasizing the importance of (a) a maintained balance of organisms in the adult, (b) breast feeding in infants to establish a large population of bifidobacteria and (c) the presence of Lactobacillus organisms to maintain healthful conditions in the human vagina. The use of Lactobacillus organisms in intestinal and vaginal disease therapy is reviewed as well as the important ecological role that lactic acid bacteria play in the natural scheme where man is concerned.

scholarly journals Effect of Cornstalk Biochar Immobilized Bacteria on Ammonia Reduction in Laying Hen Manure Composting

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1560 ◽  
Author(s):  
Huaidan Zhang ◽  
Jeremy N. Marchant-Forde ◽  
Xinyi Zhang ◽  
Yan Wang
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Relative Abundance ◽  
Nitrogen Loss ◽  
Germination Rate ◽  
Laying Hen ◽  
Water Soluble ◽  
Aerobic Composting ◽  
Nh3 Emission ◽  
Mixed Bacteria

NH3 emission has become one of the key factors for aerobic composting of animal manure. It has been reported that adding microbial agents during aerobic composting can reduce NH3 emissions. However, environmental factors have a considerable influence on the activity and stability of the microbial agent. Therefore, this study used cornstalk biochar as carriers to find out the better biological immobilization method to examine the mitigation ability and mechanism of NH3 production from laying hen manure composting. The results from different immobilized methods showed that NH3 was reduced by 12.43%, 5.53%, 14.57%, and 22.61% in the cornstalk biochar group, free load bacteria group, mixed load bacteria group, and separate load bacteria group, respectively. Under the simulated composting condition, NH3 production was 46.52, 38.14, 39.08, and 30.81 g in the treatment of the control, mixed bacteria, cornstalk biochar, and cornstalk biochar separate load immobilized mixed bacteria, respectively. The cornstalk biochar separate load immobilized mixed bacteria treatment significantly reduced NH3 emission compared with the other treatments (p < 0.05). Compared with the control, adding cornstalk biochar immobilized mixed bacteria significantly decreased the electrical conductivity, water-soluble carbon, total nitrogen loss, and concentration of ammonium nitrogen (p < 0.05), and significantly increased the seed germination rate, total number of microorganisms, and relative abundance of lactic acid bacteria throughout the composting process (p < 0.05). Therefore, the reason for the low NH3 emission might be due not only to the adsorption of the cornstalk biochar but also because of the role of complex bacteria, which increases the relative abundance of lactic acid bacteria and promotes the acid production of lactic acid bacteria to reduce NH3 emissions. This result revealed the potential of using biological immobilization technology to reduce NH3 emissions during laying hen manure composting.

scholarly journals Additive Type Affects Fermentation, Aerobic Stability and Mycotoxin Formation during Air Exposure of Early-Cut Rye (Secale cereale L.) Silage

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1432
Author(s):  
Horst Auerbach ◽  
Peter Theobald
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Water Soluble ◽  
Potassium Sorbate ◽  
Soluble Carbohydrate ◽  
Chemical Additives ◽  
Air Exposure ◽  
Carbohydrate Concentration ◽  
Aerobic Stability ◽  
Silage Production

Whole-crop rye harvested before maturity represents a valuable forage for silage production. Due to the scarcity of data on fermentation characteristics and aerobic stability (ASTA) and the lack of information on mycotoxin formation during aeration of early-cut rye (ECR) silage after silo opening, we evaluated the effects of different additive types and compositions. Wilted forage was treated with various biological and chemical additives, ensiled in 1.5-L glass jars and stored for 64 days. Fermentation pattern, yeast and mould counts and ASTA were determined at silo opening. In total 34 mycotoxins were analysed in wilted forage and in silage before and after 240 h of air exposure. Chemical additives caused the lowest dry matter (DM) losses during fermentation accompanied with the lowest ethanol production and the highest water-soluble carbohydrate concentration. Aerobic deterioration, which started within two days after silo opening in silage left untreated and inoculated with homofermentative lactic acid bacteria, was prevented by the combined use of hetero- and homofermentative lactic acid bacteria and the chemical additive containing sodium nitrite, hexamethylene tetramine and potassium sorbate. Moreover, these two additives largely restricted the formation of the mycotoxin roquefortine C to < 0.05 mg kg−1 DM after aeration, whereas untreated silage contained 85.2 mg kg−1 DM.

scholarly journals Valorization of Mushroom By-Products as a Source of Value-Added Compounds and Potential Applications

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2672 ◽  
Author(s):  
Filipa Antunes ◽  
Sara Marçal ◽  
Oludemi Taofiq ◽  
Alcina M. M. B. Morais ◽  
Ana Cristina Freitas ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Food System ◽  
Animal Feed ◽  
Value Added ◽  
Pharmaceutical Formulations ◽  
Extraction Methods ◽  
Industrial Wastes ◽  
Added Value ◽  
Future Research ◽  
By Products

Nowadays, the food sector is highly concerned with environmental issues and foreseen to develop strategies to reduce waste and losses resulting from activities developed in the food system. An approach is to increment added value to the agro-industrial wastes, which might provide economic growth and environmental protection, contributing to a circular economy. Mushroom by-products represent a disposal problem, but they are also promising sources of important compounds, which may be used due to their functional and nutritional properties. Research has been developed in different fields to obtain value added solutions for the by-products generated during mushroom production and processing. Bioactive compounds have been obtained and applied in the development of nutraceutical and pharmaceutical formulations. Additionally, other applications have been explored and include animal feed, fertilizer, bioremediation, energy production, bio-based materials, cosmetics and cosmeceuticals. The main purpose of this review is to highlight the relevant composition of mushroom by-products and discuss their potential as a source of functional compounds and other applications. Future research needs to explore pilot and industrial scale extraction methods to understand the technological feasibility and the economic sustainability of the bioactive compounds extraction and valorization towards different applications.

scholarly journals The size and shape of three water-soluble, non-ionic polysaccharides produced by lactic acid bacteria: A comparative study

2016 ◽  
Vol 142 ◽  
pp. 91-97 ◽  
Author(s):  
Marianne Øksnes Dalheim ◽  
Nina Bjørk Arnfinnsdottir ◽  
Göran Widmalm ◽  
Bjørn E. Christensen
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Comparative Study ◽  
Water Soluble ◽  
Size And Shape

scholarly journals Torrefied biomass in biofuel production system

2020 ◽  
Vol 93 (8) ◽  
pp. 9-12
Author(s):  
D. Ciolkosz ◽  
Keyword(s):  
Supply Chain ◽  
Large Scale ◽  
Crop Residues ◽  
Production Systems ◽  
Animal Feed ◽  
Energy Content ◽  
Value Added ◽  
Power Production ◽  
Biofuel Production ◽  
Added Value

Ukraine produces large amounts of crop residues every year, much which could be utilized to produce biofuel. However, efficient supply chains and system configurations are needed to make such systems efficient and cost effective. One option is to integrate torrefaction, power production and biofuel production into a single, coordinated system. This approach allows for high value product (i.e. biofuel), greater utilization of the energy content of the feedstock, and supply chain efficiency. Initial analyses indicate that revenues can be enhanced through this approach, and further analyses and optimization efforts could identify a sustainable approach to renewable fuel and power production for Ukraine. The question of scale and layout remains of interest as well, and a thorough logistical study is needed to identify the most suitable configuration. Agricultural operations often benefit from smaller scales of operation, whereas fuel production processes tend to operate profitably only at very large scale. Thus, a balance must be struck between the needs of both ends of the supply chain. The processing center concept helps to balance those needs. A system such as this also has potential to synergize with other agricultural production systems, such as the production of animal feed, fertilizer, and other bio-based products. The complexities of the Ukrainian agricultural market will need to be reflected carefully in any model that seeks to assess the system's potential. Presents a concept for coupling thermal pretreatment (torrefaction with biofuel and power production for the transformation of wheat straw into a value added product for Ukraine. Torrefaction provides supply chain savings, while conversion provides added value to the product. This paradigm has potential to utilize a widely produced waste material into a valuable source of energy and possibly other products for the country.

scholarly journals Pengaruh Penambahan Molases dan Isolat Bakteri Asam Laktat dari Cairan Rumen Sapi PO Terhadap Kualitas Silase Rumput Gajah (Pennisetum purpureum)

2014 ◽  
Vol 14 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Ismail Jasin
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Content ◽  
Animal Feed ◽  
Rumen Fluid ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Carbohydrate Source ◽  
Grass Silage ◽  
Lactic Acid Content

(The effect of molasses and lactic acid bacteria isolated from rumen fluid of PO cattle on napier grass silage quality)ABSTRACT. The objective of this study was to evaluate the effect of molasses as carbohydrate source and inoculums of lactic acid bacteria (LAB) incubated from PO cattle’s rumen fluid on the quality of Napier grass (pennisetum purpureum) silages. The research was conducted at Gemawang village Jambu District Semarang Regency. Feed analysis was carried out in Laboratory Biochemical Nutrition, Animal Feed Science, Animal Science Faculty, Gadjah Mada University. This study was assigned into Completely Randomized Design with 4 treatments and 3 replicated. The treatments were addition of molasses and LAB level into the Napier grass; 0, 1, 3 and 5% (w/w) and incubated for 30 days Data were analyzed using analysis of variance (ANOVA) and the significant effect was tested by Duncan ,s Multiple Range Test. The results showed that the addition of 1 – 5% molases significantly affected (P0,05) lactic acid content, pH, and NH3 concentration. Increasing level of molases significantly (P0,05) increased lactic acid content, decreased pH and NH3 concentration of Napier grass silage. However, among the treatment groups of 1, 3 and 5 % of molases was not significant (P0,05) different on the lactic acid content, pH and NH3 concentration. Dry matter and organic matter content of Napier grass silage were not significantly (P0,05) affected by treatments . 

WILDIERS' BIOS AND THE LACTIC ACID BACTERIA: THE RELATION OF BIOS TO THE WATER-SOLUBLE B-VITAMINS

1938 ◽  
Vol 16b (2) ◽  
pp. 46-53 ◽  
Author(s):  
Blythe Alfred Eagles ◽  
Olga Okulitch ◽  
Arthur Stephen Kadzielawa
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Chemical Properties ◽  
Water Soluble ◽  
Vitamin B ◽  
Physical And Chemical Properties ◽  
Heat Stable ◽  
Physical And Chemical ◽  
Growth Of Animals ◽  
And Chemical Properties

The influence of three distinct activators prepared from tomatoes, yeast, or liver, on the metabolism of two species of lactic acid bacteria has been studied. One of these activators is Bios II A, and the other two have been shown to be components constituting Bios II B. On the basis of their physical and chemical properties, it is suggested that the growth stimulants required by the lactic acid bacteria are identical with certain of the heat-stable accessory food factors of the Vitamin-B complex essential for the growth of animals.

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