scholarly journals Effects of Different Temperatures on the Antibacterial, Immune and Growth Performance of Crucian Carp Epidermal Mucus

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 66
Author(s):  
Bin Wang ◽  
Guoxin Ma ◽  
Yong Liu ◽  
Yafei Wang ◽  
Xiaoxue Du ◽  
...  

Fish is one of the important sources of energy and protein, and proper water temperature is key to successful fish breeding. The authors of this study evaluated crucian carp growth, mucus antibacterial properties, and immune indicators at 17, 21, 24, 27, and 31 °C. The results indicated that in the range of 17–31 °C, the resistance of epidermal mucus to Vibrio harveyi decreased with temperature rising. At 24 and 27 °C, the activities of lysozyme and catalase significantly increased; alkaline phosphatase activity, superoxide dismutase activity, and total protein concentration first increased and then decreased with rising temperature; the highest values were observed at 24 °C, with increases of 56.55%, 26.64%, and 44.52%, respectively, compared to those under the 17 °C treatment. When the treatment reached 27 °C, the temperature had an effect on the growth and antibacterial properties of crucian carp, and the activities of alkaline phosphatase and superoxide dismutase were significantly reduced. At temperatures of 17–24 °C, the survival rate of crucian carp could reach more than 93%, and at the temperature of 24 °C, the specific growth rate reached the highest value of 43.29%. Therefore, the most favorable temperature for the long-term breeding of crucian carp was found to be 24 °C. This study provides a favorable experimental basis for the establishment of intelligent aquaculture systems and the setting of water environment parameters.

1977 ◽  
Vol 16 (01) ◽  
pp. 30-35 ◽  
Author(s):  
N. Agha ◽  
R. B. R. Persson

SummaryGelchromatography column scanning has been used to study the fractions of 99mTc-pertechnetate, 99mTcchelate and reduced hydrolyzed 99mTc in preparations of 99mTc-EDTA(Sn) and 99mTc-DTPA(Sn). The labelling yield of 99mTc-EDTA(Sn) chelate was as high as 90—95% when 100 μmol EDTA · H4 and 0.5 (Amol SnCl2 was incubated with 10 ml 99mTceluate for 30—60 min at room temperature. The study of the influence of the pH-value on the fraction of 99mTc-EDTA shows that pH 2.8—2.9 gave the best labelling yield. In a comparative study of the labelling kinetics of 99mTc-EDTA(Sn) and 99mTc- DTPA(Sn) at different temperatures (7, 22 and 37°C), no significant influence on the reduction step was found. The rate constant for complex formation, however, increased more rapidly with increased temperature for 99mTc-DTPA(Sn). At room temperature only a few minutes was required to achieve a high labelling yield with 99mTc-DTPA(Sn) whereas about 60 min was required for 99mTc-EDTA(Sn). Comparative biokinetic studies in rabbits showed that the maximum activity in kidneys is achieved after 12 min with 99mTc-EDTA(Sn) but already after 6 min with 99mTc-DTPA(Sn). The long-term disappearance of 99mTc-DTPA(Sn) from the kidneys is about five times faster than that for 99mTc-EDTA(Sn).


2015 ◽  
Vol 66 (3) ◽  
pp. 265-274 ◽  
Author(s):  
Y Cao ◽  
ÉM Neif ◽  
W Li ◽  
J Coppens ◽  
N Filiz ◽  
...  

Author(s):  
A. V. Sukhova ◽  
E. N. Kryuchkova

The influence of general and local vibration on bone remodeling processes is investigated. The interrelations between the long - term exposure of industrial vibration and indicators of bone mineral density (T-and Z-criteria), biochemical markers of bone formation (osteocalcin, alkaline phosphatase) and bone resorption (ionized calcium, calcium/creatinine) were established.


Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1885
Author(s):  
Xinyu Wu ◽  
Feng Yang ◽  
Jian Gan ◽  
Zhangqian Kong ◽  
Yan Wu

The silver particles were grown in situ on the surface of wood by the silver mirror method and modified with stearic acid to acquire a surface with superhydrophobic and antibacterial properties. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and X-ray energy spectroscopy (XPS) were used to analyze the reaction mechanism of the modification process. Scanning electron microscopy (SEM) and contact angle tests were used to characterize the wettability and surface morphology. A coating with a micro rough structure was successfully constructed by the modification of stearic acid, which imparted superhydrophobicity and antibacterial activity to poplar wood. The stability tests were performed to discuss the stability of its hydrophobic performance. The results showed that it has good mechanical properties, acid and alkali resistance, and UV stability. The durability tests demonstrated that the coating has the function of water resistance and fouling resistance and can maintain the stability of its hydrophobic properties under different temperatures of heat treatment.


Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2154
Author(s):  
Amir Hussain Idrisi ◽  
Abdel-Hamid I. Mourad ◽  
Muhammad M. Sherif

This paper presents a long-term experimental investigation of E-glass/epoxy composites’ durability exposed to seawater at different temperatures. The thermoset composite samples were exposed to 23 °C, 45 °C and 65 °C seawater for a prolonged exposure time of 11 years. The mechanical performance as a function of exposure time was evaluated and a strength-based technique was used to assess the durability of the composites. The experimental results revealed that the tensile strength of E-glass/epoxy composite was reduced by 8.2%, 29.7%, and 54.4% after immersion in seawater for 11 years at 23 °C, 45 °C, and 65 °C, respectively. The prolonged immersion in seawater resulted in the plasticization and swelling in the composite. This accelerated the rate of debonding between the fibers and matrix. The failure analysis was conducted to investigate the failure mode of the samples. SEM micrographs illustrated a correlation between the fiber/matrix debonding, potholing, fiber pull-out, river line marks and matrix cracking with deterioration in the tensile characteristics of the thermoset composite.


Zygote ◽  
2019 ◽  
Vol 27 (6) ◽  
pp. 432-435
Author(s):  
Thais Rose dos Santos Hamilton ◽  
Gabriela Esteves Duarte ◽  
José Antonio Visintin ◽  
Mayra Elena Ortiz D’Ávila Assumpção

SummaryLong-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.


Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 26
Author(s):  
Sara Metwally ◽  
Daniel P. Ura ◽  
Zuzanna J. Krysiak ◽  
Łukasz Kaniuk ◽  
Piotr K. Szewczyk ◽  
...  

Atopic dermatitis (AD) is a chronic, inflammatory skin condition, caused by wide genetic, environmental, or immunologic factors. AD is very common in children but can occur at any age. The lack of long-term treatments forces the development of new strategies for skin regeneration. Polycaprolactone (PCL) is a well-developed, tissue-compatible biomaterial showing also good mechanical properties. In our study, we designed the electrospun PCL patches with controlled architecture and topography for long-term release in time. Hemp oil shows anti-inflammatory and antibacterial properties, increasing also the skin moisture without clogging the pores. It can be used as an alternative cure for patients that do not respond to traditional treatments. In the study, we tested the mechanical properties of PCL fibers, and the hemp oil spreading together with the release in time measured on skin model and human skin. The PCL membranes are suitable material as patches or bandages, characterized by good mechanical properties and high permeability. Importantly, PCL patches showed release of hemp oil up to 55% within 6 h, increasing also the skin moisture up to 25%. Our results confirmed that electrospun PCL patches are great material as oil carriers indicating a high potential to be used as skin patches for AD skin treatment.


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