The Potential Antimicrobial Action of Human Mucin 7 15-Mer Peptide and Its Metal Complexes

Mucin 7 (encoded byMUC7) is a human salivary protein that has a role in the natural immune system. Fragments of mucin 7 exhibit antimicrobial activity against bacteria and yeast. Although the antimicrobial properties of peptides have been known and studied for decades, the exact mechanism of action of antimicrobial peptides (AMPs) is still unclear. It is known that some AMPs require divalent metal ions to activate their activity. Herein, we investigated three 15-mer MUC7 peptides, one of which (mother peptide, sequence, L3) is a synthetic analog of a fragment naturally excised from MUC7 (with His3, His8, and His 14) and its two structural analogs, containing only two histidine residues, His3, His13 and His8, His13 (L2 and L1, respectively). Since there is a correlation between lipophilicity, the presence of metal ions (such as Cu(II) and Zn(II)) and antimicrobial activity of AMP, antimicrobial properties of the studied peptides, as well as their complexes with Cu(II) and Zn(II) ions, were tested for activity against Gram-positive (Enterococcus faecalis, Staphylococcus epidermidis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria and fungi (Candida albicans). The results were correlated with their lipophilicity. Coordination and thermodynamic studies (potentiometry, UV-Vis, CD) revealed the formation of mainly mononuclear complexes in solution for all studied systems with different stability in the physiological pH range.

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Antimicrobial Activity of Snake β-Defensins and Derived Peptides

Toxins ◽

10.3390/toxins14010001 ◽

2021 ◽

Vol 14 (1) ◽

pp. 1

Author(s):

Nancy Oguiura ◽

Poliana Garcia Corrêa ◽

Isabella Lemos Rosmino ◽

Ana Olívia de Souza ◽

Kerly Fernanda Mesquita Pasqualoto

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Micrococcus Luteus ◽

Peptide Sequence ◽

Tryptophan Residue ◽

High Antimicrobial Activity ◽

Bacteria And Fungi ◽

Microbroth Dilution ◽

And Staphylococcus Aureus

β-defensins are antimicrobial peptides presenting in vertebrate animals. They participate in innate immunity, but little is known about them in reptiles, including snakes. Although several β-defensin genes were described in Brazilian snakes, their function is still unknown. The peptide sequence from these genes was deduced, and synthetic peptides (with approximately 40 amino acids and derived peptides) were tested against pathogenic bacteria and fungi using microbroth dilution assays. The linear peptides, derived from β-defensins, were designed applying the bioisosterism strategy. The linear β-defensins were more active against Escherichia coli, Micrococcus luteus, Citrobacter freundii, and Staphylococcus aureus. The derived peptides (7–14 mer) showed antibacterial activity against those bacteria and on Klebsiella pneumoniae. Nonetheless, they did not present activity against Candida albicans, Cryptococcus neoformans, Trychophyton rubrum, and Aspergillus fumigatus showing that the cysteine substitution to serine is deleterious to antifungal properties. Tryptophan residue showed to be necessary to improve antibacterial activity. Even though the studied snake β-defensins do not have high antimicrobial activity, they proved to be attractive as template molecules for the development of antibiotics.

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Antimicrobial Activity of Selected Mosses on Obafemi Awolowo University Campus, Ile-Ife, Nigeria

Notulae Scientia Biologicae ◽

10.15835/nsb11310439 ◽

2019 ◽

Vol 11 (3) ◽

pp. 462-466

Author(s):

Kehinde O. OLASOJI ◽

Amos M. MAKINDE ◽

Bolajoko A. AKINPELU ◽

Oluwatoyin A. IGBENEGHU ◽

Musibau O. ISA

Keyword(s):

Antimicrobial Activity ◽

Oil Palm ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Significant Activity ◽

Moss Species ◽

Palm Wine ◽

Bacteria And Fungi ◽

Pharmacologically Active

The present study aimed to evaluate antimicrobial activity of ethanol, methanol, schnapp (40% alcohol), oil palm wine and Raffia palm wine extracts of moss species Archidium ohioense, Pelekium gratum and Hyophila involuta against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida pseudotropicalis. The antimicrobial activities of the alcoholic extracts were tested against selected microorganisms using agar well diffusion method. Minimum inhibitory concentrations (MIC) of the extracts were determined using standard methods. The antimicrobial test of the extracts on selected organisms revealed that the methanolic and ethanolic extracts of the mosses studied were inactive against all the bacteria and fungi screened, while the schnapp, Oil palm wine and Raffia palm wine extracts showed significant activity against the selected organisms. The minimum inhibitory concentration (MIC) value of the extracts on the test organisms ranged from 1.25 to 40 mg/ml. The study concluded that the extracts of the mosses studied contain pharmacologically active constituents which may be responsible for their antimicrobial properties.

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Light-Stimulated Generation of Free Radicals by Quinones-Chelators

Zeitschrift für Physikalische Chemie ◽

10.1515/zpch-2016-0831 ◽

2017 ◽

Vol 231 (2) ◽

Cited By ~ 2

Author(s):

Irina D. Markova ◽

Nikolay E. Polyakov ◽

Olga Yu. Selyutina ◽

Lidia G. Fedenok ◽

Kirill Yu. Fedotov ◽

...

Keyword(s):

Metal Ions ◽

Nuclear Polarization ◽

Anticancer Agent ◽

Reduction Potential ◽

Redox Activity ◽

Optical Absorption Spectroscopy ◽

Synthetic Analog ◽

Divalent Metal Ions ◽

Chemically Induced

AbstractThe role of metal ions in the mechanism of light-stimulated redox activity of potential anticancer agent 2-phenyl-4-(butylamino)naphtha[2,3-h]quinoline-7,12-dione (Qc) has been studied by CIDNP (chemically induced dynamic nuclear polarization) and EPR methods. The photo-induced oxidation of NADH and its synthetic analog – substituted dihydropyridine (DHP) – by quinone Qc was used as a model. The Qc capability of producing chelating complexes with divalent metal ions of Fe, Zn and Ca was studied quantitatively by optical absorption spectroscopy. A significant decrease of electrochemical reduction potential of Qc (Δ

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Development and Characterization of Weft-Knitted Fabrics of Naturally Occurring Polymer Fibers for Sustainable and Functional Textiles

Polymers ◽

10.3390/polym13040665 ◽

2021 ◽

Vol 13 (4) ◽

pp. 665

Author(s):

Marcela Ferrándiz ◽

Eduardo Fages ◽

Sandra Rojas-Lema ◽

Juan Ivorra-Martinez ◽

Jaume Gomez-Caturla ◽

...

Keyword(s):

Antimicrobial Activity ◽

Textile Industry ◽

Mechanical Performance ◽

Natural Fibers ◽

Antimicrobial Properties ◽

Fiber Formation ◽

Knitted Fabrics ◽

Functional Textiles ◽

Naturally Occurring ◽

Bacteria And Fungi

This study focuses on the potential uses in textiles of fibers of soy protein (SP) and chitin, which are naturally occurring polymers that can be obtained from agricultural and food processing by-products and wastes. The as-received natural fibers were first subjected to a three-step manufacturing process to develop yarns that were, thereafter, converted into fabrics by weft knitting. Different characterizations in terms of physical properties and comfort parameters were carried out on the natural fibers and compared to waste derived fibers of coir and also conventional cotton and cotton-based fibers, which are widely used in the textile industry. The evaluation of the geometry and mechanical properties revealed that both SP and chitin fibers showed similar fineness and tenacity values than cotton, whereas coir did not achieve the expected properties to develop fabrics. In relation to the moisture content, it was found that the SP fibers outperformed the other natural fibers, which could successfully avoid variations in the mechanical performance of their fabrics as well as impair the growth of microorganisms. In addition, the antimicrobial activity of the natural fibers was assessed against different bacteria and fungi that are typically found on the skin. The obtained results indicated that the fibers of chitin and also SP, being the latter functionalized with biocides during the fiber-formation process, showed a high antimicrobial activity. In particular, reductions of up to 100% and 60% were attained for the bacteria and fungi strains, respectively. Finally, textile comfort was evaluated on the weft-knitted fabrics of the chitin and SP fibers by means of thermal and tactile tests. The comfort analysis indicated that the thermal resistance of both fabrics was similar to that of cotton, whereas their air permeability was higher, particularly for chitin due to its higher fineness, which makes these natural fibers very promising for summer clothes. Both the SP and chitin fabrics also presented relatively similar values of fullness and softness than the pure cotton fabric in terms of body feeling and richness. However, the cotton/polyester fabric was the only one that achieved a good range for uses in winter-autumn cloths. Therefore, the results of this work demonstrate that non-conventional chitin and SP fibers can be considered as potential candidates to replace cotton fibers in fabrics for the textile industry due to their high comfort and improved sustainability. Furthermore, these natural fibers can also serve to develop novel functional textiles with antimicrobial properties.

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Synthesis and antibacterial activity of 3-arylaminomethyl-1-(2-oxo-2-arylethyl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a] azepin-1-ium bromides and aryl-(4-R1-phenyl-5,6,7,8-tetrahydro-2,2a,8a-triazacyclopenta[cd]azulen-1-ylmethyl)-amines

ScienceRise Pharmaceutical Science ◽

10.15587/2519-4852.2021.249480 ◽

2021 ◽

pp. 51-57

Author(s):

Nataliya Demchenko ◽

Zinaida Suvorova ◽

Yuliia Fedchenkova ◽

Tamara Shpychak ◽

Oleh Shpychak ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Antimicrobial Drugs ◽

Reference Drug ◽

Gram Negative ◽

In Vivo Experiments ◽

Yeast Fungi ◽

Methods Of Synthesis ◽

Bacteria And Fungi

The aim of this work is to develop methods of synthesis of 3-arylaminomethyl-1-(2-oxo-2-arylethyl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepin-1-ium bromides and aryl-(4-R1-phenyl-5,6,7,8-tetrahydro-2,2a,8a-triazacyclopenta[cd]azulen-1-ylmethyl)-amines and to study their antimicrobial activity against strains of gram-positive and gram-negative bacteria as well as yeast fungi. Materials and methods. 1Н NMR spectra were recorded on Bruker 400 spectrometer operating at frequency of 400 MHz. Antimicrobial activity of the compounds synthesized was evaluated by their minimum inhibitory concentration (MIC) values. Results and discussion. The interaction of 3-arylaminomethyl-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepines with substituted phenacyl bromides produced novel 3-arylaminomethyl-1-(2-oxo-2-arylethyl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepin-1-ium bromides. The latter when refluxed in 10 % solution of NaOH gave aryl-(4-R1-phenyl-5,6,7,8-tetrahydro-2,2a,8a-triazacyclopenta[cd]azulen-1-ylmethyl)-amines. The study of antimicrobial activity of the compounds obtained allowed to find derivatives which are active against С. albicans and S. aureus strains. Among the compounds tested 3-[(41-bromophenylamino)-methyl]-1-[2-(4-methoxyphenyl)-2-oxoethyl]-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepin-1-ium bromide 5cd appeared to be more active than the reference drug Cefixime and displayed close antimicrobial activity as the antibiotic Linezolid. Conclusions. It was found out that derivatives of 3-arylaminomethyl-1-(2-oxo-2-arylethyl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepin-1-ium bromides display broad spectrum of antimicrobial activity and are able to inhibit growth of both bacteria and fungi. S. aureus and C. albicans turned out to be the most sensitive strains to the compounds tested, MIC was in the range of 6.2-25.0 mg/mL. Gram-negative strains of microorganisms were less sensitive to the compounds evaluated and 5fа was the most active derivative displaying antimicrobial activity at the concentration of 50.0 mg/mL. Antimicrobial activity of triazoloazepinium bromide derivatives was similar to that one of Linezolid and Fluconazole reference drugs and more pronounced than the activity of Cefixime. Hence, the data gathered evidence the feasibility of further study of the antimicrobial properties of the most active compounds in in vivo experiments aiming at assessment of the prospects for the creation of new effective and safe antimicrobial drugs based on them

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In VitroAntimicrobial Screening of Alkaloid Fractions fromStrychnos potatorum

E-Journal of Chemistry ◽

10.1155/2009/535643 ◽

2009 ◽

Vol 6 (4) ◽

pp. 1200-1204 ◽

Cited By ~ 2

Author(s):

P. B. Mallikharjuna ◽

Y. N. Seetharam

Keyword(s):

Antimicrobial Activity ◽

Mycobacterium Tuberculosis ◽

Candida Albicans ◽

Folk Medicine ◽

Antimicrobial Properties ◽

Proteus Vulgaris ◽

Gram Negative ◽

Microbial Infections ◽

Bacteria And Fungi ◽

Strychnos Potatorum

Alkaloid fractions isolated fromStrychnos potatorumL.f. (Loganiaceae) seed were tested for their antimicrobial properties against some pathogenic gram positive, gram negative and acid-fast bacteria and fungi. These fractions have shown considerable antimicrobial activity against both bacteria and fungi at the tested concentrations (100 & 200 μg/mL). Further, the growth ofProteus vulgaris, taphylococcus aureus, Salmonella typhimurium, Vibrio cholerae, Mycobacterium tuberculosis, Aspergillus nigerandCandida albicanswere significantly inhibited. These findings have confirmed the use of this plant in treating of several microbial infections both in traditional and folk medicine in India.

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Improving the Antimicrobial and Mechanical Properties of Epoxy Resins via Nanomodification: An Overview

Molecules ◽

10.3390/molecules26175426 ◽

2021 ◽

Vol 26 (17) ◽

pp. 5426

Author(s):

Roberta Bertani ◽

Alessandra Bartolozzi ◽

Alessandro Pontefisso ◽

Marino Quaresimin ◽

Michele Zappalorto

Keyword(s):

Mechanical Properties ◽

Antimicrobial Activity ◽

Composite Laminates ◽

Epoxy Composite ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Comprehensive Overview ◽

Anticorrosion Coatings ◽

Bacteria And Fungi ◽

Dental Applications

The main purpose of this work is to provide a comprehensive overview on the preparation of multifunctional epoxies, with improved antimicrobial activity and enhanced mechanical properties through nanomodification. In the first section, we focus on the approaches to achieve antimicrobial activity, as well as on the methods used to evaluate their efficacy against bacteria and fungi. Relevant application examples are also discussed, with particular reference to antifouling and anticorrosion coatings for marine environments, dental applications, antimicrobial fibers and fabrics, and others. Subsequently, we discuss the mechanical behaviors of nanomodified epoxies with improved antimicrobial properties, analyzing the typical damage mechanisms leading to the significant toughening effect of nanomodification. Some examples of mechanical properties of nanomodified polymers are provided. Eventually, the possibility of achieving, at the same time, antimicrobial and mechanical improvement capabilities by nanomodification with nanoclay is discussed, with reference to both nanomodified epoxies and glass/epoxy composite laminates. According to the literature, a nanomodified epoxy can successfully exhibit antibacterial properties, while increasing its fracture toughness, even though its tensile strength may decrease. As for laminates—obtaining antibacterial properties is not followed by improved interlaminar properties.

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Interaction of divalent metal ions with the NADP+-malic enzyme from maize leaves

Physiologia Plantarum ◽

10.1034/j.1399-3054.1991.810403.x ◽

1991 ◽

Vol 81 (4) ◽

pp. 462-466 ◽

Cited By ~ 2

Author(s):

Maria Fabiana Drincovich ◽

Alberto A. Iglesias ◽

Carlos S. Andreo

Keyword(s):

Metal Ions ◽

Malic Enzyme ◽

Divalent Metal ◽

Divalent Metal Ions ◽

Maize Leaves

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Disinfection of Fruits with Activated Water

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v10i0.8462 ◽

2019 ◽

Vol 10 ◽

pp. 1864-1872

Author(s):

Prof. Teodora P. Popova

Keyword(s):

Antimicrobial Activity ◽

Aqueous Solutions ◽

Antimicrobial Properties ◽

The Other ◽

Gram Negative Bacteria ◽

High Antimicrobial Activity ◽

Gram Negative ◽

E Coli ◽

Number Of Microorganisms ◽

Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

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A Highly Crystalline Anthracene-Based MOF-74 Series Featuring Electrical Conductivity and Luminescence

10.26434/chemrxiv.8222492 ◽

2019 ◽

Author(s):

Patricia Scheurle ◽

Andre Mähringer ◽

Andreas Jakowetz ◽

Pouya Hosseini ◽

Alexander Richter ◽

...

Keyword(s):

Electrical Conductivity ◽

Metal Ions ◽

Light Emission ◽

Block Design ◽

High Surface ◽

Visible Spectrum ◽

Building Blocks ◽

Divalent Metal Ions ◽

Conductivity Enhancement ◽

Surface Areas

Recently, a small group of metal-organic frameworks (MOFs) has been discovered featuring substantial charge transport properties and electrical conductivity, hence promising to broaden the scope of potential MOF applications in fields such as batteries, fuel cells and supercapacitors. In combination with light emission, electroactive MOFs are intriguing candidates for chemical sensing and optoelectronic applications. Here, we incorporated anthracene-based building blocks into the MOF-74 topology with five different divalent metal ions, that is, Zn2+, Mg2+, Ni2+, Co2+ and Mn2+, resulting in a series of highly crystalline MOFs, coined ANMOF-74(M). This series of MOFs features substantial photoluminescence, with ANMOF-74(Zn) emitting across the whole visible spectrum. The materials moreover combine this photoluminescence with high surface areas and electrical conductivity. Compared to the original MOF-74 materials constructed from 2,5-dihydroxy terephthalic acid and the same metal ions Zn2+, Mg2+, Ni2+, Co2+ and Mn2+, we observed a conductivity enhancement of up to six orders of magnitude. Our results point towards the importance of building block design and the careful choice of the embedded MOF topology for obtaining materials with desired properties such as photoluminescence and electrical conductivity.

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