scholarly journals A Snapshot Picture of the Fungal Composition of Bee Bread in Four Locations in Bulgaria, Differing in Anthropogenic Influence

2021 ◽  
Vol 7 (10) ◽  
pp. 845
Author(s):  
Svetoslav G. Dimov ◽  
Lyuben Zagorchev ◽  
Mihail Iliev ◽  
Tereza Dekova ◽  
Ralitza Ilieva ◽  
...  

Information about the fungal composition of bee bread, and the fermentation processes to which the fungi contribute significantly, is rather scarce or fragmentary. In this study, we performed an NGS-based metagenomics snapshot picture study of the fungal composition of bee bread in four locations in Bulgaria during the most active honeybee foraging period at the end of June 2020. The sampling locations were chosen to differ significantly in climatic conditions, landscape, and anthropogenic pressure, and the Illumina 2 × 250 paired-end reads platform was used for amplicon metagenomics study of the ITS2 region. We found that some of the already reported canonical beneficial core fungal species were present within the studied samples. However, some fungal genera such as Monilinia, Sclerotinia, Golovinomyces, Toxicocladosporium, Pseudopithomyces, Podosphaera and Septoriella were reported for the first time among the dominant genera for a honeybee related product. Anthropogenic pressure negatively influences the fungal composition of the bee bread in two different ways—urban/industrial pressure affects the presence of pathogenic species, while agricultural pressure is reflected in a decrease of the ratio of the beneficial fungi.

2019 ◽  
pp. 42-44
Author(s):  
I.V. GUSAROV ◽  
V.A. OSTAPENKO ◽  
T.V. NOVIKOVА

Впервые в мире создана популяция зубров на территории 60 градусов северной широты. В новых климатических условиях разведения и сохранения зубров определены и проанализированы факторы существования вида на севере Европейской части РФ. Выявлены признаки, динамика численности, которые являются составной частью системы, предназначенной для управления биоразнообразием. Интродукция, являясь процессом введения в экосистему нехарактерных для нее видов, может усиливать изменения биоценозов как положительно, так и отрицательно. Насколько быстро и успешно проходит процесс адаптации заселенного вида, и усматривается его влияние на окружающую среду зависит дальнейшее существование зубров и в целом биоразнообразия. В статье обсуждаются вопросы взаимоотношения зубров с другими видами копытных и хозяйственной деятельностью человека, а также дальнейшим использованием зубров в сельскохозяйственном производстве. Пластичность зубров, выявление изменений и их анализ при вселении видов в новые условия обитания необходимы не только для определения развития или деградации биоценозов и в целом экосистемы, но и прогноза социально-экономических последствий интродукции как одного из методов сохранения редких и исчезающих видов фауны.For the first time in the world, a bison population has been created in an area of 60 degrees north latitude. In the new climatic conditions of breeding and preservation of bison, the factors of the species existence in the north of the European part of the Russian Federation are identified and analyzed. The signs, dynamics of abundance, which are an integral part of the system designed to manage biodiversity are identified, since the preservation of biological diversity on the planet is one of the main problems of our time. Introduction, being the process of introducing non-typical species into an ecosystem, can enhance changes in biocenoses, both positively and negatively. The question posing sounds especially when it comes to such a large hoofed animal as the European bison. How quickly and successfully the process of adaptation of the universe takes place and its environmental impact is seen depends on the continued existence of bison and biodiversity in general. The article discusses the relationship of bison with other types of ungulates and human activities, as well as the further use of bison in agricultural production. How these issues will be resolved positively depends on the future of these animals. Thus, the plasticity of bison, the identification of changes and their analysis, with the introduction of species into new habitat conditions is necessary not only to determine the development or degradation of biocenoses and the ecosystem as a whole, but also to predict the socio-economic consequences due to the introduction as one of the methods of preserving rare and endangered species of fauna.


Diversity ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 9
Author(s):  
Alexandra Grossi ◽  
Heather Proctor

When a species colonizes a new area, it has the potential to bring with it an array of smaller-bodied symbionts. Rock Pigeons (Columba livia Gmelin) have colonized most of Canada and are found in almost every urban center. In its native range, C. livia hosts more than a dozen species of ectosymbiotic arthropods, and some of these lice and mites have been reported from Rock Pigeons in the United States. Despite being so abundant and widely distributed, there are only scattered host-symbiont records for rock pigeons in Canada. Here we sample Rock Pigeons from seven locations across Canada from the west to east (a distance of > 4000 km) to increase our knowledge of the distribution of their ectosymbionts. Additionally, because ectosymbiont abundance can be affected by temperature and humidity, we looked at meteorological variables for each location to assess whether they were correlated with ectosymbiont assemblage structure. We found eight species of mites associated with different parts of the host’s integument: the feather dwelling mites Falculifer rostratus (Buchholz), Pterophagus columbae (Sugimoto) and Diplaegidia columbae (Buchholz); the skin mites: Harpyrhynchoides gallowayi Bochkov, OConnor and Klompen, H. columbae (Fain), and Ornithocheyletia hallae Smiley; and the nasal mites Tinaminyssus melloi (Castro) and T. columbae (Crossley). We also found five species of lice: Columbicola columbae (Linnaeus), Campanulotes compar (Burmeister), Coloceras tovornikae Tendeiro, Hohorstiella lata Piaget, and Bonomiella columbae Emerson. All 13 ectosymbiont species were found in the two coastal locations of Vancouver (British Columbia) and Halifax (Nova Scotia). The symbiont species found in all sampling locations were the mites O. hallae, H. gallowayi, T. melloi and T. columbae, and the lice Colu. columbae and Camp. compar. Three local meteorological variables were significantly correlated with mite assemblage structure: annual minimum and maximum temperatures and maximum humidity in the month the pigeon was collected. Two local meteorological variables, annual maximum and average temperatures, were significantly correlated with louse assemblages. Our results suggest that milder climatic conditions may affect richness and assemblage structure of ectosymbiont assemblages associated with Rock Pigeons in Canada.


Geosciences ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 323
Author(s):  
Galina Vorobieva ◽  
Nadezhda Vashukevich ◽  
Natalia Berdnikova ◽  
Ivan Berdnikov ◽  
Dmitry Zolotarev ◽  
...  

The time of Sartan glaciation in the Baikal–Yenisei Siberia, is comparable with that of MIS 2 and the deglaciation phase MIS 1. Loess loams, aeolian–colluvial sands and sandy loams represent subaerial sediments. There are four subhorizons (sr1, sr2, sr3 and sr4) in the Sartan horizon (sr). Sedimentary and soil-forming processes at different stratigraphic levels are considered. Differing soil formation types of cold periods are distinguished. Soils of the interstadial type with the A-C profile are represented only in the Early Sartan section of this paper. The soils of the pleniglacial type are discussed throughout the section. Their initial profile is O-C, TJ-C and W-C. Plant detritus remnants or poor thin humus horizons are preserved in places from the upper horizons. We propose for the first time for the interphasial soil formation type of cold stages to be distinguished. This is represented in the sections by the preserved BCm, BCg, Cm and Cg horizons of 15–20 cm thick. The upper horizons are absent in most sections. According to the surviving fragments, these were organogenous (O, TJ and T) and organomineral (AO and W) horizons. The sedimentation and soil formation features are considered from the perspective reconstruction of the Sartan natural and climatic conditions. Buried Sartan soils often contain cultural layers. Soil formation shows a well-defined periodicity of natural condition stabilization, which allowed ancient populations to adapt actively to various situations. Archaeologists’ interest in fossil soils is based on the ability of soils to “record” information about the natural and climatic conditions of human habitation.


Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2949
Author(s):  
Juan I. Burneo ◽  
Ángel Benítez ◽  
James Calva ◽  
Pablo Velastegui ◽  
Vladimir Morocho

Chemical compositions of plants are affected by the initial nutrient contents in the soil and climatic conditions; thus, we analyzed for the first time the effects of soil and leaf nutrients on the compositions of the essential oils (EOs) of Siparuna muricata in four different localities in Ecuador. EOs were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) and a gas chromatography/flame ionization detector (GC/FID). Enantiomeric distribution by GC/MS was determined, modifying the enantiomeric separation of β-pinene, limonene, δ-elemene, β-bourbonene, cis-cadina-1 (6), 4-diene and atractylone. A total of 44 compounds were identified. The most representative for L1 were guaiol, atractylone and 4-diene; for L2, cis-cadina-1(6),4-diene and myrcene; for L3, atractylone, myrcene and germacrene B; and finally, L4 germacrene B, myrcene and cis-cadina-1(6),4-diene. Correlations between soil- leaf chemical elements such as Al, Ca, Fe, Mg, Mn, N and Si in the different localities were significant with chemical composition of the essential oil of Siparuna muricata; however, correlations between soil and leaf K, P, and Na were not significant. Cluster and NMDS analysis showed high dissimilarity values of secondary metabolites between four localities related with changes in soil- leaf nutrients. Thus, the SIMPER routine revealed that not all secondary metabolites contribute equally to establishing the differences in the four localities, and the largest contributions are due to differences in guaiol, cis-cadina-1(6),4-diene, atractylone and germacrene. Our investigation showed for the first time the influences of altitude and soil- leaf chemical elements in the chemical composition of the EOs of S. muricata.


2017 ◽  
Vol 83 (17) ◽  
Author(s):  
Francesca De Filippis ◽  
Manolo Laiola ◽  
Giuseppe Blaiotta ◽  
Danilo Ercolini

ABSTRACT Target-gene amplicon sequencing is the most exploited high-throughput sequencing application in microbial ecology. The targets are taxonomically relevant genes, with 16S rRNA being the gold standard for bacteria. As for fungi, the most commonly used target is the internal transcribed spacer (ITS). However, the uneven ITS length among species may promote preferential amplification and sequencing and incorrect estimation of their abundance. Therefore, the use of different targets is desirable. We evaluated the use of three different target amplicons for the characterization of fungal diversity. After an in silico primer evaluation, we compared three amplicons (the ITS1-ITS2 region [ITS1-2], 18S ribosomal small subunit RNA, and the D1/D2 domain of the 26S ribosomal large subunit RNA), using biological samples and a mock community of common fungal species. All three targets allowed for accurate identification of the species present. Nevertheless, high heterogeneity in ITS1-2 length was found, and this caused an overestimation of the abundance of species with a shorter ITS, while both 18S and 26S amplicons allowed for more reliable quantification. We demonstrated that ITS1-2 amplicon sequencing, although widely used, may lead to an incorrect evaluation of fungal communities, and efforts should be made to promote the use of different targets in sequencing-based microbial ecology studies. IMPORTANCE Amplicon-sequencing approaches for fungi may rely on different targets affecting the diversity and abundance of the fungal species. An increasing number of studies will address fungal diversity by high-throughput amplicon sequencing. The description of the communities must be accurate and reliable in order to draw useful insights and to address both ecological and biological questions. By analyzing a mock community and several biological samples, we demonstrate that using different amplicon targets may change the results of fungal microbiota analysis, and we highlight how a careful choice of the target is fundamental for a thorough description of the fungal communities.


2018 ◽  
Vol 10 (2) ◽  
pp. 11312
Author(s):  
R. R. Rachana ◽  
R. Varatharajan

Caliothrips punctipennis (Hood) and the male of Western Flower Thrips Frankliniella occidentalis (Pergande) have been recorded for the first time from India.  F. occidentalis was collected on the leaves of Erythrina indica from Ooty in the Nilgiris, the Western Ghats, southern India, whereas C. punctipennis was collected from yellow pan traps laid at Great Nicobar, India.  Considering the quarantine importance of the pest F. occidentalis, the report of the male for the first time in India needs attention and concern.  Males are also known to be more effective vectors of tospoviruses than females.  Xerochrysum bracteatum, the Common Golden Everlasting Daisy, is often taken out of Ooty by tourists to other parts of the country.  This along with other planting materials carried by tourists and farmers could aid in the dispersal of F. occidentalis to the temperate regions of northern and southern India, where it is likely to thrive in the congenial climatic conditions prevalent there.  Under these circumstances, it is imperative that quarantine mechanisms within the country are activated and strengthened, to prevent the spread of this notorious pest to the rest of India from the pockets of its occurrence in southern India - particularly the Nilgiris.  The diagnostic characters of both species are discussed. 


Author(s):  

Cooling reservoirs of thermal power plants represent the special class of natural/ engineering systems with, on the one hand, natural geo/systems as one subsystem and, on the other hand, production/technological units as the other subsystem. Studying of these ecosystems’ functioning regularities seems to be helpful for water quality management and rational organization of water use. Besides, it is topical due to the low level of the relevant knowledge in the North of the Western Siberia. The authors for the first time in the Middle Ob Region have carried out a two-year session of monitoring of hydro/chemical indicators and temperature regime of the thermal power plants cooling reservoirs in the riparian stripe of water area. All previous investigations were done only by industrial water users within the frameworks of production monitoring in terms of three indicators in three points of the cooling reservoir water area downstream the power plant dam. Spatial distribution of concentrations of biogenic ions, salt composition, pH, organic matter, and metals in specific natural/technological system of the Surgut hthermal power plants’ reservoirs has been analyzed for the first time for the conditions of the Western Siberian taiga zone. Sources of anthropogenic pressure upon a water body have been identified. According the results of the indicators’ spatial distribution analysis three sections of the Surgut reservoir water area have been separated: a background section of the Chernaya River; a section upstream the dam with predominantly recreational character of the anthropogenic pressure; and a sectio0n downstream the dam with anthropogenic pressure of technological origin. According the outcomes of the correlation analysis indicators of the anthropogenic pressure character have been reveald.


Algologia ◽  
2020 ◽  
Vol 30 (4) ◽  
pp. 440-451
Author(s):  
M. Shamina ◽  

Cyanobacteria are organisms which play a vital role in various molecular and biotechnological aspects in food industry, agriculture, pharmaceuticals, neutraceuticals, biofuel production, etc., it is necessary to understand its adaptability to various environmental conditions. Furthermore it is equally important to discover new cyanobacterial taxa and with it occasional changes in taxonomic classification, thus the author set out to study cyanobacteria in extreme climatic conditions of desert, where temperatures are mostly above 45 oC. The taxonomic composition of cyanobacteria of Masirah Island, Sultanate of Oman, was studied for the first time. The studied samples were collected during the period of 2017–2019. The ten samples belonged to two orders: Oscillatoriales Schaffner and Synechococcales L.Hoffmann, Komárek & J.Kastovsky. All of them were filamentous non-heterocyst forms. Three species belonged to the genus Leptolyngbya Anagn. & Komárek, the genera Oscillatoria Vaucher ex Gomont and Lyngbya C.Agardh ex Gomont were represented by two species each, while the genera Pseudanabena Lauterborn, Planktolyngbya Anagn. & Komárek and Geitlerinema (Anagn. & Komárek) Anagn. were one species.


Mycotaxon ◽  
2021 ◽  
Vol 136 (2) ◽  
pp. 523-528
Author(s):  
Mustafa Kocakaya
Keyword(s):  

Three lichenicolous fungal species, Didymocyrtis epiphyscia, Lichenochora weillii, and Lichenoconium xanthoriae, are reported for the first time from Turkey. Comments on the taxonomy, ecology, and hosts and photographic illustrations are provided for each species.


2021 ◽  
Vol 157 ◽  
pp. 16204-16212

Objectif : La présente étude a pour objectif d’évaluer les paramètres de production des animaux Nélores afin d’améliorer la conduite de ces derniers élevés pour la première fois au Congo. Méthodologie et résultats : Des données portant sur le poids des veaux de la naissance à 7 mois, le gain moyen quotidien des veaux, les poids post sevrages de 12 à 36 mois, les poids moyens et les rendements à l’abattage, ont été collectées sur 40 veaux, 40 vêlles, 80 taurillons, 42 génisses et 78 taureaux. Les animaux ont été nourries d’herbes telles que Bulbostylis laniceps, Trachypogon spicatus, Ctenium newtonii, Hyparrhenia wombaliensis sur pâturages naturel et Panicum massaye, Brachiara brizenta, Brachiara omidicola, Bracharia Laneiro sur pâturages artificiel. Leur alimentation a été supplémentée par le complément minérale azoté vitaminé et complément minéral vitaminé. Les veaux naissent avec un poids 27,97±0,58 kg contre 26,88±0,61 kg pour les vêlles. Les mâles naissent plus lourds que les femelles. Cet effet d’écart de poids s’amplifie jusqu’au 7è mois où l’on enregistre 140,63±1,20 kg et 131,45±3,41 kg respectivement pour les mâles et femelles, le dimorphisme sexuel est bien prononcé. Les gains moyens quotidiens des mâles et femelles de la naissance au sevrage sont respectivement de 536,48g/j et 497,95g/j. Le suivi post sevrage montre que les Nélores mâles et femelles atteignent 171,37±4,78 kg et 158,44±13,11kg à 12 mois. Ce poids est de 349,96±8,82 kg et 289,23±29,16 kg et de 418,97± 25,84 kg et 333,67±33,12 kg respectivement pour les mâles et femelles à 24 et 36 mois. Les animaux sont abattus à l’âge de 60 ± 6,3 mois avec un poids moyen de 484,64±23,4 kg. Le rendement net à l’abattage est de 55,5±18,7%. Conclusion et application des résultats : l’adaptation productive des Nélores aux espèces fourragères du ranch Kila prouve qu’ils s’adaptent bien aux conditions climatiques du Congo et constitue un atout considérable pour le développement de l’élevage bovin au Congo. Mots clés : Adaptation, Productive, Condition climatique, Nélore, Congo ABSTRACT Objective: The objective of this study is to evaluate the production parameters of Nélores animals in order to improve the behavior of the latter reared for the first time in Congo. Methodology and results: Data on the weight of calves from birth to 7 months, average daily gain of calves, post-weaning weights from 12 to 36 months, average weights and slaughter yields were collected. on 40 Dimi Ngatse et al., J. Appl. Biosci. 2021 Adaptation productive de la race Nélore au ranch Kila République du Congo 16205 calves, 40 calves, 80 bulls, 42 heifers and 78 bulls. The animals were fed grasses such as Bulbostylis laniceps, Trachypogon spicatus, Ctenium newtonii, Hyparrhenia wombaliensis on natural pastures and Panicum massaye, Brachiara brizenta, Brachiara omidicola, Bracharia Laneiro on artificial pastures. Their diet was supplemented by the vitamin nitrogen mineral supplement and vitamin mineral supplement. Calves are born with a weight of 27.97 ± 0.58 kg against 26.88 ± 0.61 kg for calves. Males are born heavier than females. This weight difference effect is amplified until the 7th month when 140.63 ± 1.20 kg and 131.45 ± 3.41 kg are recorded respectively for males and females, the sexual dimorphism is quite pronounced. . The average daily gains of males and females from birth to weaning are respectively 536.48g / d and 497.95g / d. Post-weaning monitoring shows that male and female Nelores reach 171.37 ± 4.78 kg and 158.44 ± 13.11 kg at 12 months. This weight is 349.96 ± 8.82 kg and 289.23 ± 29.16 kg and 418.97 ± 25.84 kg and 333.67 ± 33.12 kg respectively for males and females at 24 and 36 month. The animals are slaughtered at the age of 60 ± 6.3 months with an average weight of 484.64 ± 23.4 kg. The net slaughter yield is 55.5 ± 18.7%. Conclusion and application of the results: The productive adaptation of the Nelores to the forage species of the Kila ranch proves that they adapt well to the climatic conditions of Congo and constitutes a considerable asset for the development of cattle breeding in Congo. Keywords : Adaptation, Productive, Climatic condition, Nélore, Congo


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