The Differential Phosphorylation-Dependent Signaling and Glucose Immunometabolic Responses Induced during Infection by Salmonella Enteritidis and Salmonella Heidelberg in Chicken Macrophage-like cells

Salmonella is a burden to the poultry, health, and food safety industries, resulting in illnesses, food contamination, and recalls. Salmonella enterica subspecies enterica Enteritidis (S. Enteritidis) is one of the most prevalent serotypes isolated from poultry. Salmonella enterica subspecies enterica Heidelberg (S. Heidelberg), which is becoming as prevalent as S. Enteritidis, is one of the five most isolated serotypes. Although S. Enteritidis and S. Heidelberg are almost genetically identical, they both are capable of inducing different immune and metabolic responses in host cells to successfully establish an infection. Therefore, using the kinome peptide array, we demonstrated that S. Enteritidis and S. Heidelberg infections induced differential phosphorylation of peptides on Rho proteins, caspases, toll-like receptors, and other proteins involved in metabolic- and immune-related signaling of HD11 chicken macrophages. Metabolic flux assays measuring extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) demonstrated that S. Enteritidis at 30 min postinfection (p.i.) increased glucose metabolism, while S. Heidelberg at 30 min p.i. decreased glucose metabolism. S. Enteritidis is more invasive than S. Heidelberg. These results show different immunometabolic responses of HD11 macrophages to S. Enteritidis and S. Heidelberg infections.

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First Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Enteritidis Isolated from the Chicken Meat in Russia

Proceedings ◽

10.3390/iecge-07154 ◽

2020 ◽

Vol 76 (1) ◽

pp. 2

Author(s):

Alexey V. Rakov ◽

Anatoly A. Yakovlev ◽

Viacheslav V. Sinkov

Keyword(s):

Genome Size ◽

Genome Sequence ◽

Salmonella Enterica ◽

Salmonella Enteritidis ◽

Reference Strain ◽

Draft Genome ◽

Noncoding Rnas ◽

Gallus Domesticus ◽

Gallus Gallus Domesticus ◽

Zoonotic Pathogens

Salmonella enterica subsp. enterica serovar Enteritidis is one of the most common zoonotic pathogens. We report here the genome sequence of Salmonella enterica subsp. enterica serovar Enteritidis S-25048 isolated from chicken (Gallus gallus domesticus) meat in Artyom, Russia. The assembled genome size was 4,695,145 bp. A total of 4565 coding genes, four rRNAs, 62 tRNAs, and 14 noncoding RNAs were predicted. To our knowledge, this is the first publically deposited annotated genome of this serovar isolated in Russia. The Salmonella Enteritidis S-25048 genome is suitable for use as a reference strain of Salmonella Enteritidis isolated in Russia.

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Rhythmic glucose metabolism regulates the redox circadian clockwork in human red blood cells

Nature Communications ◽

10.1038/s41467-020-20479-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Ratnasekhar Ch ◽

Guillaume Rey ◽

Sandipan Ray ◽

Pawan K. Jha ◽

Paul C. Driscoll ◽

...

Keyword(s):

Glucose Metabolism ◽

Red Blood Cells ◽

Blood Cells ◽

Mammalian Cells ◽

Metabolic Flux ◽

Pentose Phosphate ◽

Human Red Blood Cells ◽

Integral Process ◽

Metabolic Oscillations ◽

Human Rbcs

AbstractCircadian clocks coordinate mammalian behavior and physiology enabling organisms to anticipate 24-hour cycles. Transcription-translation feedback loops are thought to drive these clocks in most of mammalian cells. However, red blood cells (RBCs), which do not contain a nucleus, and cannot perform transcription or translation, nonetheless exhibit circadian redox rhythms. Here we show human RBCs display circadian regulation of glucose metabolism, which is required to sustain daily redox oscillations. We found daily rhythms of metabolite levels and flux through glycolysis and the pentose phosphate pathway (PPP). We show that inhibition of critical enzymes in either pathway abolished 24-hour rhythms in metabolic flux and redox oscillations, and determined that metabolic oscillations are necessary for redox rhythmicity. Furthermore, metabolic flux rhythms also occur in nucleated cells, and persist when the core transcriptional circadian clockwork is absent in Bmal1 knockouts. Thus, we propose that rhythmic glucose metabolism is an integral process in circadian rhythms.

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Multiplication in Egg Yolk and Survival in Egg Albumen of Salmonella enterica Serotype Enteritidis Strains of Phage Types 4, 8, 13a, and 14b

Journal of Food Protection ◽

10.4315/0362-028x-64.6.865 ◽

2001 ◽

Vol 64 (6) ◽

pp. 865-868 ◽

Cited By ~ 29

Author(s):

RICHARD K. GAST ◽

PETER S. HOLT

Keyword(s):

Salmonella Enterica ◽

Salmonella Enteritidis ◽

Egg Yolk ◽

Phage Types ◽

Egg Albumen ◽

Range Of Values ◽

Salmonella Enterica Serotype Enteritidis

Refrigeration of eggs is vital for restricting the multiplication of Salmonella enterica serotype Enteritidis contaminants, but differences between Salmonella Enteritidis strains or phage types in their survival and multiplication patterns in egg contents might influence the effectiveness of refrigeration standards. The present study compared the abilities of 12 Salmonella Enteritidis isolates of four phage types (4, 8, 13a, and 14b) to multiply rapidly in egg yolk and to survive for several days in egg albumen. The multiplication of very small numbers of Salmonella Enteritidis inoculated into yolk (approximately 101 CFU/ml) was monitored during 24 h of incubation at 25°C, and the survival of much larger numbers of Salmonella Enteritidis inoculated into albumen (approximately 105 CFU/ml) was similarly evaluated during the first 3 days of incubation at the same temperature. In yolk, the inoculated Salmonella Enteritidis strains multiplied to mean levels of approximately 103 CFU/ml after 6 h of incubation and 108 CFU/ml after 24 h. In albumen, mean levels of approximately 104 CFU/ml or more of Salmonella Enteritidis were maintained through 72 h. Although a few differences in multiplication and survival were observed between individual isolates, the overall range of values was relatively narrow, and no significant differences (P < 0.05) were evident among phage types.

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Inactivation of Escherichia coli O157:H7, Salmonella enterica Serotype Enteritidis, and Listeria monocytogenes on Lettuce by Hydrogen Peroxide and Lactic Acid and by Hydrogen Peroxide with Mild Heat

Journal of Food Protection ◽

10.4315/0362-028x-65.8.1215 ◽

2002 ◽

Vol 65 (8) ◽

pp. 1215-1220 ◽

Cited By ~ 107

Author(s):

CHIA-MIN LIN ◽

SARAH S. MOON ◽

MICHAEL P. DOYLE ◽

KAY H. McWATTERS

Keyword(s):

Escherichia Coli ◽

Hydrogen Peroxide ◽

Lactic Acid ◽

Listeria Monocytogenes ◽

Salmonella Enterica ◽

Salmonella Enteritidis ◽

Sensory Characteristics ◽

Escherichia Coli O157 ◽

E Coli ◽

Log Reduction

Iceberg lettuce is a major component in vegetable salad and has been associated with many outbreaks of foodborne illnesses. In this study, several combinations of lactic acid and hydrogen peroxide were tested to obtain effective antibacterial activity without adverse effects on sensory characteristics. A five-strain mixture of Escherichia coli O157:H7, Salmonella enterica serotype Enteritidis, and Listeria monocytogenes was inoculated separately onto fresh-cut lettuce leaves, which were later treated with 1.5% lactic acid plus 1.5% hydrogen peroxide (H2O2) at 40°C for 15 min, 1.5% lactic acid plus 2% H2O2 at 22°C for 5 min, and 2% H2O2 at 50°C for 60 or 90 s. Control lettuce leaves were treated with deionized water under the same conditions. A 4-log reduction was obtained for lettuce treated with the combinations of lactic acid and H2O2 for E. coli O157:H7 and Salmonella Enteritidis, and a 3-log reduction was obtained for L. monocytogenes. However, the sensory characteristics of lettuce were compromised by these treatments. The treatment of lettuce leaves with 2% H2O2 at 50°C was effective not only in reducing pathogenic bacteria but also in maintaining good sensory quality for up to 15 days. A ≤4-log reduction of E. coli O157:H7 and Salmonella Enteritidis was achieved with the 2% H2O2 treatment, whereas a 3-log reduction of L. monocytogenes was obtained. There was no significant difference (P > 0.05) between pathogen population reductions obtained with 2% H2O2 with 60- and 90-s exposure times. Hydrogen peroxide residue was undetectable (the minimum level of sensitivity was 2 ppm) on lettuce surfaces after the treated lettuce was rinsed with cold water and centrifuged with a salad spinner. Hence, the treatment of lettuce with 2% H2O2 at 50°C for 60 s is effective in initially reducing substantial populations of foodborne pathogens and maintaining high product quality.

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Multiplication and Motility of Salmonella enterica Serovars Enteritidis, Infantis, and Montevideo in In Vitro Contamination Models of Eggs

Journal of Food Protection ◽

10.4315/0362-028x-69.5.1012 ◽

2006 ◽

Vol 69 (5) ◽

pp. 1012-1016 ◽

Cited By ~ 15

Author(s):

TOSHIYUKI MURASE ◽

KAZUHIKO FUJIMOTO ◽

RUI NAKAYAMA ◽

KOICHI OTSUKI

Keyword(s):

Salmonella Enterica ◽

Salmonella Enteritidis ◽

Egg Yolk ◽

Vitelline Membrane ◽

Human Patient ◽

Polycarbonate Membrane ◽

Specific Pathogen ◽

Egg Albumen ◽

Petri Plate

The invasive ability of Salmonella enterica serovars Enteritidis, Infantis, and Montevideo in eggs was examined. Strains of these serovars originating from egg contents, laying chicken houses, and human patients were experimentally inoculated (0.1-ml dose containing 78 to 178 cells) onto the vitelline membrane of eggs collected from specific-pathogen-free chickens and incubated at 25°C. The test strains were detected in 25 of 138 yolk contents by day 6, indicating the penetration of Salmonella organisms through the vitelline membrane. There were no significant differences in overall rates of penetration between serovars. The organisms were also detected in the albumen from 125 of 138 eggs tested by day 6. Growth to more than 106 CFU/ml was observed in 48 of the 125 albumen samples. An inoculum of 1,000 Salmonella cells was added to 15 ml of albumen at the edge of a petri plate. A 10-mm-diameter cylindrical well, the bottom of which was sealed with a polycarbonate membrane with 3.0-μm pores, was filled with egg yolk and placed into the albumen at the center of the dish, which was maintained at 25°C. Experiments were performed in triplicate with each strain. Salmonella organisms in all the albumen samples were detected by day 11. However, motility of the organisms toward the yolk was observed in only two dishes inoculated with the Salmonella Enteritidis strain from a human patient and in one dish inoculated with the Salmonella Infantis strain from liquid egg. The albumen samples obtained from the dishes inoculated with the Salmonella Enteritidis strain had high numbers of bacteria (>108 CFU/ml). The present study suggests that Salmonella organisms in egg albumen are unlikely to actively move toward the yolk, although deposition on or near the vitelline membrane can be advantageous for proliferation.

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Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in Salmonella Enteritidis 13076 and Salmonella Typhimurium 14028

Antibiotics ◽

10.3390/antibiotics10101191 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1191

Author(s):

Yuliany Guillín ◽

Marlon Cáceres ◽

Rodrigo Torres ◽

Elena Stashenko ◽

Claudia Ortiz

Keyword(s):

Quorum Sensing ◽

Essential Oils ◽

Salmonella Enterica ◽

Salmonella Enteritidis ◽

Antimicrobial Agents ◽

Multidrug Resistant ◽

Transcriptional Analysis ◽

Bacterial Cells ◽

Sem Images ◽

Biofilm Inhibition

The emergence of multidrug-resistant microorganisms represents a global challenge that has led to a search for new antimicrobial compounds. Essential oils (EOs) from medicinal aromatic plants are a potential alternative for conventional antibiotics. In this study, the antimicrobial and anti-biofilm potential of 15 EOs was evaluated on planktonic and biofilm-associated cells of Salmonella enterica serovar Enteritidis ATCC 13076 (S. enteritidis) and Salmonella enterica serovar Typhimurium ATCC 14028 (S. typhimurium). In total, 4 out of 15 EOs showed antimicrobial activity and 6 EOs showed anti-biofilm activity against both strains. The EO from the Lippia origanoides chemotype thymol-carvacrol II (LTC II) presented the lowest minimum inhibitory concentration (MIC50 = 0.37 mg mL−1) and minimum bactericidal concentration (MBC = 0.75 mg mL−1) values. This EO also presented the highest percentage of biofilm inhibition (>65%) on both microorganisms, which could be confirmed by scanning electron microscopy (SEM) images. Transcriptional analysis showed significant changes in the expression of the genes related to quorum sensing and the formation of the biofilm. EOs could inhibit the expression of genes involved in the quorum sensing mechanism (luxR, luxS, qseB, sdiA) and biofilm formation (csgA, csgB, csgD, flhD, fliZ, and motB), indicating their potential use as anti-biofilm antimicrobial agents. However, further studies are needed to elucidate the action mechanisms of essential oils on the bacterial cells under study.

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Genotypic characterization of antibiotic-resistant Salmonella Enteritidis isolates in Dakar, Senegal

The Journal of Infection in Developing Countries ◽

10.3855/jidc.365 ◽

2007 ◽

Vol 1 (03) ◽

pp. 284-288 ◽

Cited By ~ 9

Author(s):

Amy Gassama Sow ◽

Abdoul Aziz Wane ◽

Mamadou Hadi Diallo ◽

Cheikh Saad-Bouh Boye ◽

Awa Aïdara-Kane

Keyword(s):

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Salmonella Enterica ◽

Salmonella Enteritidis ◽

Data Bank ◽

Human Infection ◽

Molecular Techniques ◽

Conjugative Plasmid ◽

Bacterial Strains ◽

Salmonella Enterica Serovar Enteritidis

Background: It is well established that Salmonella enterica is a major cause of food-borne disease worldwide. In Africa, according to the Who Global Salm-Surv country data bank from 2000 to 2002 Salmonella enterica serovar Enteritidis was the most common serotype involved in human salmonellosis. In Dakar this serotype of Salmonella has been reported as a frequent and an increasing cause of human infection. Methodology: The genetic determinants of the antimicrobial resistance of 25 selected multiresistant strains of Salmonella enterica serovar Enteritidis referred to the National Reference Center for Enterobacteria (NRCE) in Dakar were investigated using molecular techniques. Results: All strains carried blaTEM 1 genes. Five harboured three types of class 1 integrons with gene cassettes dfrA15, dfrA1-aadA1 and dfrA7. Multiresistance was due to a 23 Kb conjugative plasmid. DNA fingerprinting by macrorestriction of genomic DNA revealed a single related group suggesting that strains might be clonal. Conclusions: The spread of resistance genes through plasmid transfer plays an important role in the dissemination of antibiotic resistance in enteric pathogens such as Salmonella Enteritidis; the risk of transmissibility of antibiotic resistance between different bacterial strains highlights the urgent need to develop strategies to limit the spread of antimicrobial resistance among bacterial enteropathogens.

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Multiple Factors Independently RegulatehilA and Invasion Gene Expression in Salmonella enterica Serovar Typhimurium

Journal of Bacteriology ◽

10.1128/jb.182.7.1872-1882.2000 ◽

2000 ◽

Vol 182 (7) ◽

pp. 1872-1882 ◽

Cited By ~ 167

Author(s):

Robin L. Lucas ◽

C. Phoebe Lostroh ◽

Concetta C. DiRusso ◽

Michael P. Spector ◽

Barry L. Wanner ◽

...

Keyword(s):

Gene Expression ◽

Salmonella Enterica ◽

Inorganic Phosphate ◽

Response Regulator ◽

Regulatory System ◽

Negative Control ◽

Host Cells ◽

Uptake System ◽

Intracellular Signals ◽

Serovar Typhimurium

HilA activates the expression of Salmonella entericaserovar Typhimurium invasion genes. To learn more about regulation ofhilA, we isolated Tn5 mutants exhibiting reduced hilA and/or invasion gene expression. In addition to expected mutations, we identified Tn5 insertions inpstS, fadD, flhD, flhC, and fliA. Analysis of the pstS mutant indicates that hilA and invasion genes are repressed by the response regulator PhoB in the absence of the Pst high-affinity inorganic phosphate uptake system. This system is required for negative control of the PhoR-PhoB two-component regulatory system, suggesting thathilA expression may be repressed by PhoR-PhoB under low extracellular inorganic phosphate conditions. FadD is required for uptake and degradation of long-chain fatty acids, and our analysis of the fadD mutant indicates that hilA is regulated by a FadD-dependent, FadR-independent mechanism. Thus, fatty acid derivatives may act as intracellular signals to regulatehilA expression. flhDC and fliAencode transcription factors required for flagellum production, motility, and chemotaxis. Complementation studies with flhCand fliA mutants indicate that FliZ, which is encoded in an operon with fliA, activates expression of hilA, linking regulation of hilA with motility. Finally, epistasis tests showed that PhoB, FadD, FliZ, SirA, and EnvZ act independently to regulate hilA expression and invasion. In summary, our screen has identified several distinct pathways that can modulate S. enterica serovar Typhimurium's ability to express hilA and invade host cells. Integration of signals from these different pathways may help restrict invasion gene expression during infection.

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Characteristics of a Series of Three Bacteriophages Infecting Salmonella enterica Strains

International Journal of Molecular Sciences ◽

10.3390/ijms21176152 ◽

2020 ◽

Vol 21 (17) ◽

pp. 6152 ◽

Cited By ~ 1

Author(s):

Katarzyna Kosznik-Kwaśnicka ◽

Karolina Ciemińska ◽

Michał Grabski ◽

Łukasz Grabowski ◽

Marcin Górniak ◽

...

Keyword(s):

Salmonella Enterica ◽

Phage Therapy ◽

Functional Characterization ◽

Bacterial Biofilm ◽

Host Cells ◽

Detailed Characterization ◽

Food Protection ◽

Adsorption Rates ◽

Potential Use

Molecular and functional characterization of a series of three bacteriophages, vB_SenM-1, vB_SenM-2, and vB_SenS-3, infecting various Salmonella enterica serovars and strains is presented. All these phages were able to develop lytically while not forming prophages. Moreover, they were able to survive at pH 3. The phages revealed different host ranges within serovars and strains of S. enterica, different adsorption rates on host cells, and different lytic growth kinetics at various temperatures (in the range of 25 to 42 °C). They efficiently reduced the number of cells in the bacterial biofilm and decreased the biofilm mass. Whole genome sequences of these phages have been determined and analyzed, including their phylogenetic relationships. In conclusion, we have demonstrated detailed characterization of a series of three bacteriophages, vB_SenM-1, vB_SenM-2, and vB_SenS-3, which reveal favorable features in light of their potential use in phage therapy of humans and animals, as well as for food protection purposes.

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Nature and consequences of interactions between Salmonella enterica serovar Dublin and host cells in cattle

Veterinary Research ◽

10.1186/s13567-019-0720-5 ◽

2019 ◽

Vol 50 (1) ◽

Cited By ~ 1

Author(s):

Prerna Vohra ◽

Christina Vrettou ◽

Jayne C. Hope ◽

John Hopkins ◽

Mark P. Stevens

Keyword(s):

Lymph Nodes ◽

Salmonella Enterica ◽

Fluorescent Protein ◽

Host Cells ◽

Large Animal ◽

Ileal Mucosa ◽

Zoonotic Infections ◽

Infected Cells ◽

Green Fluorescent ◽

Salmonella Enterica Serovar Dublin

AbstractSalmonella enterica is a veterinary and zoonotic pathogen of global importance. While murine and cell-based models of infection have provided considerable knowledge about the molecular basis of virulence of Salmonella, relatively little is known about salmonellosis in naturally-affected large animal hosts such as cattle, which are a reservoir of human salmonellosis. As in humans, Salmonella causes bovine disease ranging from self-limiting enteritis to systemic typhoid-like disease and exerts significant economic and welfare costs. Understanding the nature and consequences of Salmonella interactions with bovine cells will inform the design of effective vaccines and interventions to control animal and zoonotic infections. In calves challenged orally with S. Dublin expressing green fluorescent protein (GFP) we observed that the bacteria were predominantly extracellular in the distal ileal mucosa and within gut-associated lymph nodes 48 h post-infection. Intracellular bacteria, identified by flow cytometry using the GFP signal, were predominantly within MHCII+ macrophage-like cells. In contrast to observations from murine models, these S. Dublin-infected cells had elevated levels of MHCII and CD40 compared to both uninfected cells from the same tissue and cells from the cognate tissue of uninfected animals. Moreover, no gross changes of the architecture of infected lymph nodes were observed as was described previously in a mouse model. In order to further investigate Salmonella-macrophage interactions, net replication of S. enterica serovars that differ in virulence in cattle was measured in bovine blood-derived macrophages by enumeration of gentamicin-protected bacteria and fluorescence dilution, but did not correlate with host-specificity.

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