scholarly journals Enhancing the Membranolytic Activity of Chenopodium quinoa Saponins by Fast Microwave Hydrolysis

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1731 ◽  
Author(s):  
Emmanuel Colson ◽  
Philippe Savarino ◽  
Emily J.S. Claereboudt ◽  
Gustavo Cabrera-Barjas ◽  
Magali Deleu ◽  
...  
Keyword(s):  
Hemolytic Activity ◽  
Plant Biomass ◽  
Biomedical Application ◽  
Chenopodium Quinoa ◽  
Plant Secondary Metabolites ◽  
Phospholipid Bilayer ◽  
Added Value ◽  
Microwave Assisted ◽  
Saponin Content ◽  
Microwave Hydrolysis

Saponins are plant secondary metabolites. There are associated with defensive roles due to their cytotoxicity and are active against microorganisms. Saponins are frequently targeted to develop efficient drugs. Plant biomass containing saponins deserves sustained interest to develop high-added value applications. A key issue when considering the use of saponins for human healthcare is their toxicity that must be modulated before envisaging any biomedical application. This can only go through understanding the saponin-membrane interactions. Quinoa is abundantly consumed worldwide, but the quinoa husk is discarded due to its astringent taste associated with its saponin content. Here, we focus on the saponins of the quinoa husk extract (QE). We qualitatively and quantitively characterized the QE saponins using mass spectrometry. They are bidesmosidic molecules, with two oligosaccharidic chains appended on the aglycone with two different linkages; a glycosidic bond and an ester function. The latter can be hydrolyzed to prepare monodesmosidic molecules. The microwave-assisted hydrolysis reaction was optimized to produce monodesmosidic saponins. The membranolytic activity of the saponins was assayed based on their hemolytic activity that was shown to be drastically increased upon hydrolysis. In silico investigations confirmed that the monodesmosidic saponins interact preferentially with a model phospholipid bilayer, explaining the measured increased hemolytic activity.

scholarly journals PENGELOLAAN BIOMASSA TANAMAN DALAM BIOINDUSTRI PERKEBUNAN MENDUKUNG PENGEMBANGAN BIOENERGI Plant Biomass Management in Plantations Bioindustry Supporting Bioenergy Development

Perspektif ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 135
Author(s):  
Suci Wulandari ◽  
Sumanto Sumanto ◽  
Saefudin Saefudin
Keyword(s):  
Food Systems ◽  
Production Systems ◽  
Plant Biomass ◽  
System Development ◽  
Sustainable Use ◽  
Cropping System ◽  
Added Value ◽  
Plant Parts ◽  
Use Of Resources ◽  
Plantation Crops

<p>Biomassa tanaman perkebunan dapat dimanfaatkan untuk pangan, pakan, dan bioenergi. Hasil penelitian dan perkembangan teknologi telah mendorong pemanfaatan biomassa bagian-bagian tanaman tersebut. Tanaman perkebunan memiliki potensi besar untuk menghasilkan biomassa yang dapat dimanfaatkan dalam pengembangan energi terbarukan. Pemetaan potensi biomassa telah banyak dilakukan pada tanaman perkebunan, seperti pada: tebu, kakao, kelapa sawit, kemiri sunan, jarak pagar, kopi, kelapa dalam, karet dan teh. Pengembangan sistem produksi pangan dan biomassa untuk pembangkit energi melalui sistem multi tanam berbasis komoditas perkebunan telah dikembangkan.  Di Kabupaten Aceh Timur telah dilakukan pengembangan sistem agroindustri juga memanfaatkan semua produk samping, mendorong daur ulang dan pemanfaatan residu. Pemanfaatan potensi bioenergi masih dihadapkan pada berbagai kendala distribusi, kontinuitas pasokan bahan dan aspek ekonomi. Menyikapi hal tersebut langkah strategis dapat dilakukan melalui: analisis neraca karbon, alokasi lahan, pemanfaatan lahan, pemanfaatan sumber daya secara berkelanjutan, dukungan teknologi, fokus pada nilai tambah yang tinggi dan perbaikan tata kelola. Selanjutnya perbaikan pada pengembangan sistem pangan energi terpadu dapat ditempuh melalui: (1) sosialisasi dari inovasi teknologi, (2) membentuk kawasan-kawasan pertanian terpadu di daerah sentra pengembangan dan (3) memperkuat kelembagaan petani untuk mengembangkan agroindustri.</p><p> </p><p><strong> </strong><strong> </strong>ABSTRACT</p><p align="center">Biomass from estate crops can be used for food, feed, and bioenergy. The results of research and technological developments have encouraged the utilization of biomass of these plant parts. Plantation crops have great potential to produce biomass that can be utilized in the development of renewable energy. Mapping of biomass potential has been carried out in plantation crops, such as: sugar cane, cocoa, oil palm, candlenut, jatropha, coffee, deep coconut, rubber, and tea. The development of food and biomass production systems for energy generation through a commodity-based multi-cropping system has been developed. In East Aceh District an agro-industrial system development has also been carried out utilizing all byproducts, encouraging recycling and utilizing residues. The utilization of bioenergy is still faced with various distribution constraints, continuity of material supply and economic aspects. In response to this, strategic steps can be taken through carbon balance analysis, land allocation, land use, sustainable use of resources, technology support, focus on high added value and improved governance. Furthermore, improvements to the development of integrated energy food systems can be pursued through (1) socialization of technological innovations, (2) establishing integrated agricultural areas in plant centers and (3) strengthening farmer institutions to develop agro-industries.</p><p> </p>

scholarly journals Preserving the Cellular Tissue Structure of Maize Pith Though Dry Fractionation Processes: A Key Point to Use as Insulating Agro-Materials

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5350
Author(s):  
Claire Mayer-Laigle ◽  
Laia Haurie Ibarra ◽  
Amélie Breysse ◽  
Marina Palumbo ◽  
Frédéric Mabille ◽  
...  
Keyword(s):  
Particle Size ◽  
Composite Materials ◽  
Plant Biomass ◽  
Cellular Tissue ◽  
Added Value ◽  
Vascular Bundles ◽  
Dry Fractionation ◽  
Promising Alternative ◽  
Structural Support ◽  
Electrostatic Separator

Plant biomass has various compositions and structures at different scales (from the component organs to their constitutive tissues) to support its functional properties. Recovering each part of the plant without damaging its structure poses a challenge to preserving its original properties for differential dedicated end uses, and considerably increases its added value. In this work, an original combination of grinding based on shearing stress and separation based on particle size and density was successfully used to sort rind (65% w/w) and pith (35% w/w) from maize stem internodes. More than 97% of the rind was isolated. The pith alveolar structure was well preserved in coarse particles, making them suitable for insulation bio-based composite materials, a promising alternative to conventional nonbiodegradable insulation panels. Boards produced from the dry fractionated pith exhibited thermal conductivities like those produced from hand dissected pith, with values equal to 0.037 W·mK−1 and 0.039 W·mK−1, respectively. In the finest fraction (particle size <1 mm), the pith vascular bundles (around 300–400 µm in diameter) were dissociated from parenchyma cells and successfully isolated using a cutting-edge electrostatic separator. Their structures, which provide the plant structural support, make them potentially valuable for reinforcement in composite materials.

scholarly journals Seed yield of two new quinoa (Chenopodium quinoa Willd.) breeding lines as affected by sowing date in Central Italy

2019 ◽  
Vol 113 (1) ◽  
pp. 51 ◽  
Author(s):  
Paolo CASINI
Keyword(s):  
Seed Yield ◽  
Central Italy ◽  
Chenopodium Quinoa ◽  
Sowing Date ◽  
Breeding Lines ◽  
Saponin Content ◽  
Sowing Dates ◽  
Stage Development ◽  
Sowing Period ◽  
Phenological Phases

<p>Research on the introduction of quinoa in Italy is currently lacking. The present research was aimed at identifying the correct sowing period. Field experiment was consucted in Cesa, Tuscany, in 2017. Two new breeding lines coded as DISPAA-Q42 and DISPAA-Q47-CB were utilized. Three sowing dates (SD) were implemented: February 23; March 17 and April 27. Results showed that the most successful SD was February 23. A significant decrease in both seed yield and a delay in phenological phases, relating to plant maturation and flowering was associated with the sequential delay in SD in both lines. Results also showed a significant effect of lines on yield, true-leaf stage development, flower development and maturity. Only DISPAA-Q42 was considered suitable for cultivation in the Tuscan environment. DISPAA-Q47-CB was the more susceptible line, due to the sequential delay in SD and delayed plant maturation. No effect between lines was evident for protein and saponin content. The present study clearly shows the potential for the successful cultivation of quinoa in Central Italy, and highlights the necessity of taking into consideration both breeding lines and SD in order to accomplish this goal.</p>

scholarly journals Seed and Saponin Production of Organic Quinoa (Chenopodium quinoa Willd.) for different Tillage and Fertilization

2012 ◽  
Vol 40 (1) ◽  
pp. 42 ◽  
Author(s):  
Dimitrios BILALIS ◽  
Ioanna KAKABOUKI ◽  
Anestis KARKANIS ◽  
Ilias Travlos ◽  
Vassilis TRIANTAFYLLIDIS ◽  
...  
Keyword(s):  
Chenopodium Quinoa ◽  
Dry Weight ◽  
Root Density ◽  
Soil Porosity ◽  
Cow Manure ◽  
Organic Fertilization ◽  
Area Index ◽  
Total N ◽  
Saponin Content ◽  
Tillage System

Field experiment was conducted to determine the effects of tillage systems and fertilization on growth, yield and quality of quinoa crop (Chenopodium quinoa Willd.). The experiment was laid out in a split-plot design with four replicates, two main plots [conventional tillage (CT) and minimum tillage (MT)] and three sub-plots (fertilization treatments: control, cow manure and compost). The soil porosity (45.5-49.75%) and total nitrogen (0.144-0.173%) were higher in soils subjected to MT system than under CT. In soil porosity, an interaction between fertilization and tillage system was found. The highest leaf area index (4.47-5.03), dry weight (8650-9290 kg ha-1) and root density (1.03-1.21 cm cm-3) were also found in MT. Moreover, there were significant differences between the organic fertilization treatments concerning the LAI, dry weight and root density. The highest seed yield (2485-2643 kg ha-1) and saponin content (0.42-0.45%) were found in cow manure and compost treatments. Also, the highest saponin yield (7.70-12.05 kg ha-1) was found in the MT system. Saponin yield had positive and significant correlation with total N (r=0.866). In quinoa measurements, an interaction between fertilization and tillage system was not found. The present results indicated that MT and organic fertilization increase saponin yield of quinoa.

scholarly journals Determination and Metabolite Profiling of Mixtures of Triterpenoid Saponins from Seeds of Chilean Quinoa (Chenopodium quinoa) Germplasm

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1867
Author(s):  
Archis Pandya ◽  
Björn Thiele ◽  
Andres Zurita-Silva ◽  
Björn Usadel ◽  
Fabio Fiorani
Keyword(s):  
Chenopodium Quinoa ◽  
Dry Weight ◽  
Organoleptic Properties ◽  
Breeding Programs ◽  
Triterpenoid Saponins ◽  
Mass Estimation ◽  
Fticr Ms ◽  
Genotype Effect ◽  
Saponin Content

The seed pericarp of Chenopodium quinoa Willd. (quinoa) contains a mixture of triterpenoid saponins conferring undesired organoleptic properties. In this study, we evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions. Relative saponin content ranged from 0.22 to 15.04 mg/g of seed dry weight among the genotypes studied and the genotype effect was significant (p < 0.001). About 75% of the genotypes could be classified as low-saponin content lines which is promising in view of ongoing plant breeding efforts. In addition to the quantitative determination of saponins, metabolic profiling was performed by LC-FTICR-MS and LC-MS/MS. We obtained highly accurate mass estimation from ion spectra allowing the identification of twelve saponins of the oleanane type. These differ in their aglycons and in the number and type of glycoside units. Interestingly, we identified a saponin compound that, to our knowledge, had not been reported previously. Our study highlights that there is considerable variability concerning saponin content in quinoa, which contributes to the valorization of genetic resources towards the identification of genotypes that could be utilized in current and future quinoa breeding programs.

scholarly journals Increased Tea Saponin Content Influences The Diversity and Function of Plantation Soil Microbiomes

2021 ◽  
Author(s):  
Shouke Zhang ◽  
Junqia Kong ◽  
Zikun Li ◽  
Feng Song ◽  
Xinhua He ◽  
...  
Keyword(s):  
Microbial Community ◽  
Physicochemical Properties ◽  
Cellulose Degradation ◽  
Plant Secondary Metabolites ◽  
Soil Microbiome ◽  
Soil Physicochemical Properties ◽  
Bacterial Populations ◽  
The Core ◽  
Tea Saponin ◽  
Saponin Content

Abstract Background Plant secondary metabolites (PSMs) can affect the structures and functions of soil microbiomes. However, the core bacteria associated with PSMs, and their corresponding functions have not been explored extensively. In this study, soil physicochemical properties, tea saponin contents, microbial community compositions, and microbial community functions of different-age Camellia oleifera plantation soils from representative regions were analyzed. We evaluated the effects of plantation age increase on PSM accumulation, and the subsequent consequences on the structures and functions of soil microbiomes. Results Plantation ages increase positively corresponded with accumulated tea saponin contents, with negative effects on soil physicochemical properties, and soil microbiome structures and functions. Older plantation soil microbiomes exhibited simpler structures, lower diversity, and relatively looser putative interactions based on network analysis. Clearly, the core functions of soil microbiomes transitioned to those associated with PSM metabolisms, while microbial pathways involved in cellulose degradation were inhibited. Degradation experiments further confirmed that older plantation soils exhibited the higher capacity on tea saponin degradation but poorer on furfural. Conclusions This study systematically explored the influences of PSMs on soil microbiomes via the investigation of key bacterial populations and their functional pathways. With the increase of planting years, increased tea saponin content simplified the soil microbiomes diversity, inhibited the degradation of organic matter, and enriched the genes related to the degradation of tea saponin. These findings significantly advance our understanding on PSMs-microbiome interactions and could provide fundamental and important data for sustainable management of Camellia plantations.

Predominant secretion of cellobiohydrolases and endo-β-1,4-glucanases in nutrient-limited medium by Aspergillus spp. isolated from subtropical field

2020 ◽  
Vol 168 (3) ◽  
pp. 243-256 ◽  
Author(s):  
May Thin Kyu ◽  
Shunsuke Nishio ◽  
Koki Noda ◽  
Bay Dar ◽  
San San Aye ◽  
...  
Keyword(s):  
Rice Straw ◽  
Plant Biomass ◽  
Cellulose Degradation ◽  
Industrial Wastes ◽  
Added Value ◽  
Crystalline Cellulose ◽  
Cellulolytic Activity ◽  
Biological Degradation ◽  
Industry Waste ◽  
Degradation Intermediates

Abstract Biological degradation of cellulose from dead plants in nature and plant biomass from agricultural and food-industry waste is important for sustainable carbon recirculation. This study aimed at searching diverse cellulose-degrading systems of wild filamentous fungi and obtaining fungal lines useful for cellooligosaccharide production from agro-industrial wastes. Fungal lines with cellulolytic activity were screened and isolated from stacked rice straw and soil in subtropical fields. Among 13 isolated lines, in liquid culture with a nutrition-limited cellulose-containing medium, four lines of Aspergillus spp. secreted 50–60 kDa proteins as markedly dominant components and gave clear activity bands of possible endo-β-1,4-glucanase in zymography. Mass spectroscopy (MS) analysis of the dominant components identified three endo-β-1,4-glucanases (GH5, GH7 and GH12) and two cellobiohydrolases (GH6 and GH7). Cellulose degradation by the secreted proteins was analysed by LC-MS-based measurement of derivatized reducing sugars. The enzymes from the four Aspergillus spp. produced cellobiose from crystalline cellulose and cellotriose at a low level compared with cellobiose. Moreover, though smaller than that from crystalline cellulose, the enzymes of two representative lines degraded powdered rice straw and produced cellobiose. These fungal lines and enzymes would be effective for production of cellooligosaccharides as cellulose degradation-intermediates with added value other than glucose.

Polymer Aerogels: Preparation and Potential for Biomedical Application

2021 ◽  
pp. 1-22
Keyword(s):  
Functional Properties ◽  
Biomedical Application ◽  
Added Value ◽  
Ecological Value ◽  
Chemical Modifications ◽  
Drying Techniques ◽  
Low Toxicity ◽  
Physicochemical And Functional Properties

Polymeric aerogels have high added value and application. The potential to use natural polysaccharides, especially those from waste, has contributed to adding economic, social and ecological value. This chapter seeks to put forth the latest findings on the development of polymeric aerogels, drying techniques, properties, and pharmacological applications. The functional properties of polymeric aerogels, such as biodegradability, low toxicity and biocompatibility with cellular media are addressed. In the last decade, several works have reported the production of polymeric aerogels from natural polysaccharides. Chemical modifications and filling of new molecules were studied, improving the physicochemical and functional properties of the aerogels, as well as the drying techniques, were reported, and discarded. The production of polymeric aerogels is considered strategic for the development of sustainable, biodegradable and economically viable products.

scholarly journals Plant Secondary Metabolites: An Opportunity for Circular Economy

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 495
Author(s):  
Ilaria Chiocchio ◽  
Manuela Mandrone ◽  
Paola Tomasi ◽  
Lorenzo Marincich ◽  
Ferruccio Poli
Keyword(s):  
Secondary Metabolites ◽  
Circular Economy ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Food Additives ◽  
Plant Secondary Metabolites ◽  
Industrial Sector ◽  
Industrial Wastes ◽  
Added Value ◽  
Chemical Structures

Moving toward a more sustainable development, a pivotal role is played by circular economy and a smarter waste management. Industrial wastes from plants offer a wide spectrum of possibilities for their valorization, still being enriched in high added-value molecules, such as secondary metabolites (SMs). The current review provides an overview of the most common SM classes (chemical structures, classification, biological activities) present in different plant waste/by-products and their potential use in various fields. A bibliographic survey was carried out, taking into account 99 research articles (from 2006 to 2020), summarizing all the information about waste type, its plant source, industrial sector of provenience, contained SMs, reported bioactivities, and proposals for its valorization. This survey highlighted that a great deal of the current publications are focused on the exploitation of plant wastes in human healthcare and food (including cosmetic, pharmaceutical, nutraceutical and food additives). However, as summarized in this review, plant SMs also possess an enormous potential for further uses. Accordingly, an increasing number of investigations on neglected plant matrices and their use in areas such as veterinary science or agriculture are expected, considering also the need to implement “greener” practices in the latter sector.

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