scholarly journals Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3168 ◽  
Author(s):  
Alexander Tamashevski ◽  
Yuliya Harmaza ◽  
Ekaterina Slobozhanina ◽  
Roman Viter ◽  
Igor Iatsunskyi

The precise detection of cancer cells currently remains a global challenge. One-dimensional (1D) semiconductor nanostructures (e.g., ZnO nanorods) have attracted attention due to their potential use in cancer biosensors. In the current study, it was demonstrated that the possibility of a photoluminescent detection of human leukemic T-cells by using a zinc oxide nanorods (ZnO NRs) platform. Monoclonal antibodies (MABs) anti-CD5 against a cluster of differentiation (CD) proteins on the pathologic cell surface have been used as a bioselective layer on the ZnO surface. The optimal concentration of the protein anti-CD5 to form an effective bioselective layer on the ZnO NRs surface was selected. The novel biosensing platforms based on glass/ZnO NRs/anti-CD5 were tested towards the human T-lymphoblast cell line MOLT-4 derived from patients with acute lymphoblastic leukemia. The control tests towards MOLT-4 cells were performed by using the glass/ZnO NRs/anti-IgG2a system as a negative control. It was shown that the photoluminescence signal of the glass/ZnO NRs/anti-CD5 system increased after adsorption of T-lymphoblast MOLT-4 cells on the biosensor surface. The increase in the ZnO NRs photoluminescence intensity correlated with the number of CD5-positive MOLT-4 cells in the investigated population (controlled by using flow cytometry). Perspectives of the developed ZnO platforms as an efficient cancer cell biosensor were discussed.

2008 ◽  
Vol 8 (11) ◽  
pp. 5854-5857 ◽  
Author(s):  
Guangping Zhu ◽  
Chunxiang Xu ◽  
Jing Zhu ◽  
Changgui Lu ◽  
Yiping Cui ◽  
...  

High density zinc oxide nanorods with uniform size were synthesized on (100) silicon substrate by vapor-phase transport method. The scanning electron microscopy images reveal that the nanorods have an average diameter of about 400 nm. The X-ray diffraction pattern demonstrates the wurtzite crystalline structure of the ZnO nanorods growing along [0001] direction. The single-photon excited photoluminescence presents a strong ultraviolet emission band at 394 nm and a weak visible emission band at 600 nm. When the ZnO nanorods were respectively pumped by various wavelength lasers from 520 nm to 700 nm, two-photon excited ultraviolet photoluminescence was observed. The dependence of the two-photon excited photoluminescence intensity on the excitation wavelength and power was investigated in detail.


2011 ◽  
Vol 10 (04n05) ◽  
pp. 845-849
Author(s):  
GAURAV SHUKLA ◽  
ALIKA KHARE

Hydrothermal growth of highly c-axis oriented ZnO nanorods with high aspect ratio on pulsed laser deposited ZnO seed layer is reported. Effect of pre-heating time, growth time and seed layer on the structural, morphological and optical properties of ZnO nanorods is presented. The possible growth mechanism for ZnO nanorods is also discussed.


2013 ◽  
Vol 26 ◽  
pp. 33-38 ◽  
Author(s):  
Ruziana Mohamed ◽  
Zuraida Khusaimi ◽  
A.N. Afaah ◽  
Aadila Aziz ◽  
Mohamad Hafiz Mamat ◽  
...  

Magnesium (Mg)-doped zinc oxides (ZnO) have been prepared on a silicon substrate by using the solution-immersion method. The nanorods films were annealed at different temperature 0°C, 250°C, 500°C respectively for 1 hour. The XRD diffraction indicated that the Mg-doped ZnO nanorods have good crystallinity with a hexagonal wurzite structure preferentially oriented along the (002) direction. PL spectroscopy at room temperature shows strong UV peaks appearing at 383 nm when annealed at 250°C. The intensity of broad emission peaks increases with increasing annealing temperature to 500°C which is possibility attributed to intrinsic defects.


Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 922 ◽  
Author(s):  
Francesca Luzi ◽  
Alessandro Di Michele ◽  
Luigi Torre ◽  
Debora Puglia

Poly(vinyl alcohol-co-ethylene) (EVOH) films containing zinc oxide nanorods (ZnO Nrods) at 0.1, 0.5, and 1 wt%, were realized by solvent casting. The effect of ZnO Nrods content on morphological, thermal, optical, mechanical, and oxygen permeability properties were analyzed. In addition, moisture content and accelerated-aging test studies were performed, with the intention to determine the influence of zinc oxide nanofillers on the functional characteristics of realized packaging systems. Tensile properties showed increased values for strength and deformation-at-break in EVOH-based formulations reinforced with 0.1 and 0.5 wt% of zinc oxide nanorods. Results from the colorimetric and transparency investigations underlined that the presence of ZnO Nrods in EVOH copolymer did not induce evident alterations. In addition, after the accelerated-aging test, the colorimetric test confirmed the possibility for these materials to be used in the packaging sector. This behavior was induced by the presence of zinc oxide nanofillers that act as a UV block that made them useful as an efficient absorber of UV radiation.


2014 ◽  
Vol 5 ◽  
pp. 173-179 ◽  
Author(s):  
Rafal Pietruszka ◽  
Bartlomiej Slawomir Witkowski ◽  
Grzegorz Luka ◽  
Lukasz Wachnicki ◽  
Sylwia Gieraltowska ◽  
...  

Selected properties of photovoltaic (PV) structures based on n-type zinc oxide nanorods grown by a low temperature hydrothermal method on p-type silicon substrates (100) are investigated. PV structures were covered with thin films of Al doped ZnO grown by atomic layer deposition acting as transparent electrodes. The investigated PV structures differ in terms of the shapes and densities of their nanorods. The best response is observed for the structure containing closely-spaced nanorods, which show light conversion efficiency of 3.6%.


2016 ◽  
Vol 19 (4) ◽  
pp. 181-183 ◽  
Author(s):  
Tzu-Yi Yu ◽  
Yi Cian Chen ◽  
Wang Ting Chiu ◽  
Yang Luo ◽  
Sheng Shin Wang ◽  
...  

In this study, we address process how the ZnO nanorods were deposited on GaN substrates with spin-coating by using the hydro-thermal methods. After ZnO was spin coated, the samples were annealed with different temperatures to incorporate with Au nano particles. Multiple material analyses, such as the field emission scanning electron microscopy (FESEM), the energy dispersive X-ray spectroscopy (EDX) and the X-ray diffraction (XRD) analyses were carried out to characterize the Au nanoparticles/ZnO nanorods/GaN nanocomposites.


2013 ◽  
Vol 667 ◽  
pp. 375-379 ◽  
Author(s):  
M. Awalludin ◽  
Mohamad Hafiz Mamat ◽  
Mohd Zainizan Sahdan ◽  
Z. Mohamad ◽  
Mohamad Rusop

This paper focus on zinc oxide (ZnO) nanorods prepared using sol-gel immersion method immersed at different time. Immersion times have been varied 1~24 hr and the characteristics of each sample have been observed. The effects of immersion time on ZnO nanorods thin films have been studied in surface morphology and structural properties using Scanning Electron Microscopy (SEM) and X-ray diffractometer (XRD), respectively.


2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Alojz Anžlovar ◽  
Zorica Crnjak Orel ◽  
Ksenija Kogej ◽  
Majda ¦igon

ZnO nanorods (length 30–150 nm) were synthesized in di(ethylene glycol) using Zn(CH3COO)2as a precursor andpara-toluene sulphonic acid, p-TSA, as an end-capping agent. Increasing the concentration of p-TSA above 0.1 M causes the reduction of the ZnO length. Nanocomposites with poly(methyl methacrylate) were prepared using unmodified nanorods. They enhanced the UV absorption of nanocomposites (∼98%) at low ZnO concentrations (0.05–0.1 wt.%), while visible light transparency was high. At concentrations of 1 wt.% and above, nanorods enhanced the thermal stability of nanocomposites. At low concentrations (0.05–0.1 wt.%), they increased the storage modulus of material and shiftedTgtowards higher temperatures as shown by dynamic mechanical analysis, DMA, while at higher concentrations (1.0 wt.%) this effect was deteriorated. DMA also showed that spherical ZnO particles have a more pronounced effect on the storage modulus andTgthan nanorods.


2013 ◽  
Vol 667 ◽  
pp. 388-392
Author(s):  
S.Z. Muhamed ◽  
Mohamad Hafiz Mamat ◽  
Mohamad Rusop

We investigated the effect of immersion time on optical, electrical properties and surface morphology of nanostructured Aluminium (Al) doped Zinc Oxide (ZnO) thin films prepared by immersion technique. UV-Vis-NIR spectra indicate that the transmittance of the samples decrease with immersion time. Electrical properties study reveals the nanostructured Al doped ZnO thin film at 1 hr shows the lowest resistivity compared to other samples. Surface morphology results as characterized by scanning electron microscope (SEM) show that the Al doped ZnO nanorods quantity increased with immersion time.


2008 ◽  
Vol 2008 ◽  
pp. 1-4 ◽  
Author(s):  
M. H. Asif ◽  
O. Nur ◽  
M. Willander ◽  
M. Yakovleva ◽  
B. Danielsson

Zinc oxide nanorods with 100 nm diameter and 900 nm length were grown on the surface of a silver wire (0.25 mm in diameter) with the aim to produce electrochemical nanosensors. It is shown that the ZnO nanorods exhibit a -dependent electrochemical potentiometric behavior in an aqueous solution. The potential difference was found to be linear over a large logarithmic concentration range (1 M to 0.1 M) using Ag/AgCl as a reference electrode and the response time was less than one minute. In order to adapt the sensors for calcium ion measurements in biological fluids with sufficient selectivity and stability, plastic membrane coatings containing ionophores were applied. These functionalized ZnO nanorods sensors showed a high sensitivity (26.55 mV/decade) and good stability.


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