scholarly journals The Wound-Healing Effect of Mango Peel Extract on Incision Wounds in a Murine Model

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 259
Author(s):  
Lesslie Espinosa-Espinosa ◽  
Leticia Garduño-Siciliano ◽  
Mario Rodriguez-Canales ◽  
Luis Barbo Hernandez-Portilla ◽  
Maria Margarita Canales-Martinez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Histological Analysis ◽  
Mangifera Indica ◽  
Normal Skin ◽  
Healing Process ◽  
Antibacterial Activities ◽  
Wound Contraction ◽  
Wound Healing Process ◽  
Mango Peel ◽  
Anti Inflammatory

Mangifera indica can generate up to 60% of polluting by-products, including peels. However, it has been shown that flavonoids and mangiferin are mainly responsible for the antioxidant, anti-inflammatory, and antibacterial activities closely related to the wound-healing process. The chemical composition of MEMI (methanolic extract of M. indica) was analyzed by HPLC-DAD, as well as concentrations of total phenol (TPC) and flavonoids (TFC) and antioxidant activity (SA50). Wound-healing efficacy was determined by measurements of wound contraction, histological analysis, and tensiometric method; moreover, anti-inflammatory, antibacterial, and acute dermal toxicity (OECD 402) were also evaluated. Phenol, resorcinol, conjugated resorcinol, and mangiferin were detected. TPC, TFC, and SA50 were 136 mg GAE/g, 101.66 mg QE/g, and 36.33 µg/mL, respectively. Tensile strength and wound contraction closure did not show significant differences between MEMI and dexpanthenol groups. Histological analysis (after 14 days) shows a similar architecture between MEMI treatment and normal skin. MEMI exhibits a reduction in edema. Staphylococcus epidermidis had an MIC of 2 mg/mL, while Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli reached 4 mg/mL. The MEMI showed no signs of toxicity. Therefore, this study demonstrates multiple targets that flavonoids and mangiferin of MEMI may present during the healing process.

scholarly journals Profile of wound healing process induced by allantoin

2010 ◽  
Vol 25 (5) ◽  
pp. 460-461 ◽  
Author(s):  
Lorena Ulhôa Araújo ◽  
Andrea Grabe-Guimarães ◽  
Vanessa Carla Furtado Mosqueira ◽  
Claudia Martins Carneiro ◽  
Neila Márcia Silva-Barcellos
Keyword(s):  
Wound Healing ◽  
Histological Analysis ◽  
Normal Skin ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Water Emulsion ◽  
Wound Model ◽  
Female Wistar Rats ◽  
First Time

PURPOSE: To evaluate and characterize the wound healing process profile induced by allantoin incorporated in soft lotion oil/water emulsion using the planimetric and histological methods. METHODS: Female Wistar rats (n=60) were randomly assigned to 3 experimental groups: (C) control group-without treatment; (E) group treated with soft lotion O/W emulsion excipients; (EA) group treated with soft lotion O/W emulsion containing allantoin 5%. The emulsions either containing or not allantoin were topically administered for 14 days and the wound area was evaluated by planimetry and by qualitative and quantitative histological analysis of open wound model. RESULTS: The data which were obtained and analyzed innovate by demonstrating, qualitatively and quantitatively, by histological analysis, the profile of healing process induced by allantoin. The results suggest that the wound healing mechanism induced by allantoin occurs via the regulation of inflammatory response and stimulus to fibroblastic proliferation and extracellular matrix synthesis. CONCLUSION: This work show, for the first time, the histological wound healing profile induced by allantoin in rats and demonstrated that it is able to ameliorate and fasten the reestablishment of the normal skin.

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals Wound Healing and Anti-Inflammatory Effect in Animal Models ofCalendula officinalisL. Growing in Brazil

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Leila Maria Leal Parente ◽  
Ruy de Souza Lino Júnior ◽  
Leonice Manrique Faustino Tresvenzol ◽  
Marina Clare Vinaud ◽  
José Realino de Paula ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Chorioallantoic Membrane ◽  
Ethanolic Extract ◽  
Immunohistochemical Analysis ◽  
Antibacterial Activities ◽  
Cutaneous Wounds ◽  
Annual Herb ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Calendula officinalisis an annual herb from Mediterranean origin which is popularly used in wound healing and as an anti-inflammatory agent. In this study, the ethanolic extract, the dichloromethane, and hexanic fractions of the flowers from plants growing in Brazil were produced. The angiogenic activity of the extract and fractions was evaluated through the chorioallantoic membrane and cutaneous wounds in rat models. The healing activity of the extract was evaluated by the same cutaneous wounds model through macroscopic, morphometric, histopathologic, and immunohistochemical analysis. The antibacterial activity of the extract and fractions was also evaluated. This experimental study revealed thatC. officinalispresented anti-inflammatory and antibacterial activities as well as angiogenic and fibroplastic properties acting in a positive way on the inflammatory and proliferative phases of the healing process.

scholarly journals Anti-inflammatory activity of Schrebera Swietenioides Roxb. in cream formulation on CD68 expression in mice skin wound

2019 ◽  
Vol 9 (5) ◽  
pp. 39-42
Author(s):  
Piush Sharma ◽  
Charanjeet Singh
Keyword(s):  
Wound Healing ◽  
Surgical Procedures ◽  
Healing Process ◽  
Skin Incision ◽  
Skin Wound ◽  
Inflammatory Activity ◽  
Wound Healing Process ◽  
Male Mice ◽  
Entire Surface ◽  
Anti Inflammatory

Aim: The purpose of this study was to determine the potential of Schrebera Swietenioides alcohol leaf extract in cream composition in the expression of immunoglobulin CD68 (macrophage) during the wound healing process in the inflammatory stage of mice skin. Materials and Methods: Amount of 12 two-months-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. The mice were allotted into four groups of each, entire surface of each group wound covered by base cream control, sulfadiazine 0.1% cream, alcoholic leaves extract of Schrebera Swietenioides cream 10% and, 15%, respectively. All experiments were performed twice a day for 3 days. The wound healing was assayed in stained histological sections in immunohistochemical of the wounds. CD68 expression was investigated under a microscope. Amount of 12-month-old male mice between 30 and 40 g was used. For surgical procedures, a 2.0 cm skin lesion was also performed under the skin on the vertebrae surrounding each animal. Treatment was performed under local anesthetic with procaine cream. Rats were assigned to four groups each, the entire surface of each group of lesions covered by control cream, 0.1% sulfadiazine cream, alcohol leaf from Schrebera Swietenioides cream respectively at 10% and at 15%. All tests were performed twice a day for 3 days. The lesions were analyzed in sections of tissue with staining of wound immunochemistry. The expression of CD68 was studied under a microscope. Results: The results showed that the cream from the 10% and 15% alcoholic leaves extract of Schrebera Swietenioides revealed moderate immune reaction to CD68 on wound healing. Conclusion: We concluded that the alcoholic leaves extract cream of Schrebera Swietenioides possesses anti-inflammatory activity in wound healing process of mice skin.

Recent Developments in Nano-Drug Delivery Systems Loaded by Phytochemicals for Wound Healing

2020 ◽  
Vol 20 (18) ◽  
pp. 1867-1878
Author(s):  
Neda Mirrezaei ◽  
Rezvan Yazdian-Robati ◽  
Fatemeh Oroojalian ◽  
Amirhossein Sahebkar ◽  
Maryam Hashemi
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Antibacterial Activities ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Wound Treatment ◽  
Therapeutic Modalities ◽  
Multi Stage ◽  
Recent Developments ◽  
Applicable Knowledge

Wound healing is a multi-stage process during which a cascade of molecular and cellular events collaborate to restore the damaged tissue to its healthy state. The inability of the available therapies to effectively heal the wounds has imposed major problems on healthcare systems. Therefore, developing novel therapeutic modalities capable of enhancing wound healing process with no/or limited scar formation is of more importance. Different studies have investigated the potential of phytochemicals on the wound healing process. They have shown to exert anti-inflammatory, antioxidant, and antibacterial activities as well as promoting collagen synthesis and deposition, leading to enhancing wound healing. Nanotechnology, as an applicable knowledge, has provided versatile means to improve the efficiency and effectiveness of wound treatment. The application of nanoparticles has conferred various advantages in the field of wound treatment. They protect the therapeutics from degradation, release the cargo in a controlled fashion, possess healing properties, and can act as extracellular matrix (ECM) mimic. In this review, we discuss the naturally-occurring compounds with wound healing properties and their nano-formulation for skin wound therapy.

scholarly journals The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Nenad Nikolić ◽  
Philip Kienzl ◽  
Pooja Tajpara ◽  
Martin Vierhapper ◽  
Johannes Matiasek ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Activation ◽  
Ex Vivo ◽  
Wound Care ◽  
Normal Skin ◽  
Healing Process ◽  
Negative Influence ◽  
Tape Stripping ◽  
Wound Healing Process ◽  
Skin Cells

Ideal agents for the topical treatment of skin wounds should have antimicrobial efficacy without negative influence on wound healing. Octenidine (OCT) has become a widely used antiseptic in professional wound care, but its influence on several components of the wound healing process remains unclear. In the present study, we have used a superficial wound model using tape stripping on human full-thickness skin ex vivo to investigate the influence of OCT on epidermal Langerhans cells (LCs) and cytokine secretion pattern of skin cells during wound healing in a model without disruption of the normal skin structure. Histological and immunofluorescence studies showed that OCT neither altered human skin architecture nor the viability of skin cells upon 48 hours of culture in unwounded or wounded skin. The epidermis of explants and LCs remained morphologically intact throughout the whole culture period upon OCT treatment. OCT inhibited the upregulation of the maturation marker CD83 on LCs and prevented their emigration in wounded skin. Furthermore, OCT reduced both pro- and anti-inflammatory mediators (IL-8, IL-33, and IL-10), while angiogenesis and growth factor mediators (VEGF and TGF-β1) remained unchanged in skin explant cultures. Our data provide novel insights into the host response to OCT in the biologically relevant environment of viable human (wounded) skin.

scholarly journals Study Literature Peran Epidermal Growth Factor dalam Proses Penyembuhan Luka

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Nur Febrianti ◽  
Takdir Tahir ◽  
Saldy Yusuf
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Egf Receptor ◽  
Normal Skin ◽  
Healing Process ◽  
The Body ◽  
Wound Healing Process ◽  
Epithelial Tissue ◽  
Epidermal Growth

Background: Wounds occur when normal skin structures are damaged. Injury events in Indonesia have increased by 8.2% and the highest prevalence in South Sulawesi is 12.8%. One of the growth factors that play a role in the wound healing process is epidermal growth factor (EGF). EGF includes polypeptides that contain 53 amino acids, and EGF is present in all fluids in the body and platelets. This review aims to determine the role of EGF in the process of wound healing. Method: Data collected since 2017 using 8 databases (pubmed, science direct, google schollar) where the literature used is internationally published literature, additional references are taken from the bibliography of all relevant articles, all relevant articles are reviewed and analyzed. Results: EGF has a role in wound healing. EGF increases motility and epithelial cell migration. EGF can stimulate cell growth, proliferation and differentiation by binding to high affinity to the EGF receptor (EGFR) on the cell surface. The goal of EGF healing is most epithelial tissue, fibroblasts, and endothelial cells. EGF can call three important biological actions in tissue repair including cytoprotection, mitogenesis, and migration. Conclusion: EGF plays an important role in the wound healing process, especially in the re-epithelial process. Based on this review, it is suggested that the selection of dressings that are used should support EGF.

scholarly journals Pengaruh pemberian getah bonggol pisang (Musa paradisiaca var. sapientum L. Kuntze. AAB) terhadap penyembuhan luka sayat pada kulit tikus Wistar (Rattus norvegicus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Jacqueline J. Wakkary ◽  
Meilany Durry ◽  
Carla Kairupan
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Herbal Remedy ◽  
Normal Skin ◽  
Healing Process ◽  
Fibrous Tissue ◽  
Wound Healing Process ◽  
Musa Paradisiaca ◽  
Banana Stem

Abstract: Wound healing can be assisted by modern and traditional medicine. Banana stem sap is often used as a herbal remedy for wounds. The sap of banana stem contains saponins, flavonoids, anthraquinone, quinone, lectin, and tannins that are expected to promote wound healing. This study was aimed to determine the effect of banana stem sap on incised wound of the skin of Wistar rats. This was an experimental laboratory study. A total of 21 Wistar rats (Rattus norvegicus) were used in this study; one was used to obtain a normal skin sample, and the others were divided into control groups (A1 and B1) and treated groups (A2 and B2), five rats in each group. A 1-cm-skin incision was made on the back of each rat in all groups. The wounds of group A1 and B1 were untreated. In group A2 and B2, the wounds were applied with 0,5 ml of banana stem sap (Musa paradisiaca var. sapientum L. Kuntze. AAB) twice a day. Macroscopic and microscopic examinations of the wounds were performed on day-5 (group A1 and A2) and day-14 (group B1 and B2). The results showed that, either macroscopically (wound area and crusting) or microscopically (crust, reepithelization, angiogenesis, proliferation of fibrous tissue and inflammatory cells), group A2 and B2 exhibited better wound healing compared with group A1 and B1. Conclusion: Better wound healing process in Wistar rats treated with banana stem sap on their insiced wounds compared to those that were untreated indicates that banana stem sap play distinct roles in promoting wound healing.Keywords: wound healing, banana stem sap Abstrak: Penyembuhan luka dapat dibantu dengan pengobatan modern ataupun teradisional. Masyarakat awam sering menggunakan pengobatan tradisional salah satunya getah bonggol pisang untuk mengobati luka. Getah bonggol pisang mengandung saponin, flavonoid, antrakuinon, kuinon, laktin, dan tanin yang diduga dapat membantu penyembuhan luka. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian getah bonggol pisang terhadap penyembuhan luka sayat pada kulit tikus wistar. Jenis penelitian ialah eksperimental laboratorik dengan subjek 21 ekor tikus Wistar (Rattus norvegicus) yang dibagi dalam: satu ekor tanpa perlakuan (sampel kulit normal) dan empat kelompok perlakuan (lima ekor tiap kelompok). Kelompok A1 dan B1 dibuat luka sayat ± 1 cm pada daerah punggung tetapi tidak diolesi getah bonggol pisang (Musa paradisiaca var. sapientum L. Kuntze. AAB). Kelompok A2 dan B2 dibuat luka sayat kemudian diolesi getah bonggol pisang 0,5 ml dua kali sehari. Penilaian dan pengambilan sampel jaringan kulit luka dilakukan pada hari ke-5 (kelompok A1 dan A2) dan hari ke-14 (kelompok B1 dan B2). Hasil penelitian menunjukkan bahwa gambaran penyembuhan luka, baik secara makroskopik (area permukaan luka dan kerak luka) maupun mikroskopik (krusta, reepitelisasi, angiogenesis, proliferasi jaringan ikat dan sel-sel radang) pada kelompok A2 dan B2 lebih baik dibandingkan dengan kelompok A1 dan B1. Simpulan: Ditemukan tanda-tanda penyembuhan luka yang lebih baik pada kelompok tikus Wistar yang diberikan getah bonggol pisang pada luka sayatnya dibandingkan dengan yang tidak diberikan, yang mengindikasikan bahwa getah bonggol pisang berperan khusus dalam membantu proses penyembuhan luka.Kata kunci: penyembuhan luka, getah bonggol pisang

scholarly journals Wound Healing Properties of Ethanolic Extract of Acrostichum aureum and Acrostichum speciosum Rhizome in Rats

2013 ◽  
Vol 1 (2) ◽  
pp. 42-48
Author(s):  
Hendy Putra Herman ◽  
Deny Susanti ◽  
Shahbudin Saad ◽  
Muhammad Taher ◽  
Norazsida Ramli
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Wound Contraction ◽  
Wound Healing Process ◽  
Beneficial Effects ◽  
Biopsy Punch ◽  
Excisional Wound ◽  
Negative Controls

This paper presents the assessment of wound healing properties of ethanolic extract of Acrostichum aureum and A. speciosum in rats. It was carried out on 6 treated groups with 6 rats each group. The excisional wound was made on the dorsal interscapular region of each rat by a 6 mm biopsy punch. T1 and T2 were treated with 5% and 10% A. aureum ethanolic extract in aqua cream, respectively. Meanwhile, T3 and T4 were treated with 5% and 10% A. speciosum ethanolic extract in aqua cream, respectively. Solcoseryl jelly and aqua cream were used as positive and negative controls. The treatments were applied topically twice daily, wound contraction and period of epithelization were measured every 2 days. The results showed that wound treated with 10% A. speciosum (T4) exerted faster wound contraction significantly (p < 0.05) compared to the negative control. It was also enhanced epithelization period (9.33 ± 3.20 days) of the wounds significantly (p < 0.05). In conclusion, these results strongly suggested the beneficial effects of both plant extracts for enhancing wound healing process.

scholarly journals High Efficiency In Vitro Wound Healing of Dictyophora indusiata Extracts via Anti-Inflammatory and Collagen Stimulating (MMP-2 Inhibition) Mechanisms

2021 ◽  
Vol 7 (12) ◽  
pp. 1100
Author(s):  
Yasir Nazir ◽  
Pichchapa Linsaenkart ◽  
Chiranan Khantham ◽  
Tanakarn Chaitep ◽  
Pensak Jantrawut ◽  
...  
Keyword(s):  
Wound Healing ◽  
Interleukin 1 ◽  
Healing Process ◽  
Matrix Metalloproteinase 2 ◽  
Nucleotide Sequencing ◽  
Wound Healing Process ◽  
Healing Effect ◽  
Wound Healing Effect ◽  
Anti Inflammatory ◽  
Collagen Stimulation

Dictyophora indusiata or Phallus indusiatus is widely used as not only traditional medicine, functional foods, but also, skin care agents. Biological activities of the fruiting body from D. indusiata were widely reported, while the studies on the application of immature bamboo mushroom extracts were limited especially in the wound healing effect. Wound healing process composed of 4 stages including hemostasis, inflammation, proliferation, and remodelling. This study divided the egg stage of bamboo mushroom into 3 parts: peel and green mixture (PGW), core (CW), and whole mushroom (WW). Then, aqueous extracts were investigated for their nucleotide sequencing, biological compound contents, and wound healing effect. The anti-inflammatory determination via the levels of cytokine releasing from macrophages, and the collagen stimulation activity on fibroblasts by matrix metalloproteinase-2 (MMP-2) inhibitory activity were determined to serve for the wound healing process promotion in the stage 2–4 (wound inflammation, proliferation, and remodelling of the skin). All D. indusiata extracts showed good antioxidant potential, significantly anti-inflammatory activity in the decreasing of the nitric oxide (NO), interleukin-1 (IL-1), interleukin-1 (IL-6), and tumour necrosis factor-α (TNF-α) secretion from macrophage cells (p < 0.05), and the effective collagen stimulation via MMP-2 inhibition. In particular, CW extract containing high content of catechin (68.761 ± 0.010 mg/g extract) which could significantly suppress NO secretion (0.06 ± 0.02 µmol/L) better than the standard anti-inflammatory drug diclofenac (0.12 ± 0.02 µmol/L) and their MMP-2 inhibition (41.33 ± 9.44%) was comparable to L-ascorbic acid (50.65 ± 2.53%). These findings support that CW of D. indusiata could be an essential natural active ingredient for skin wound healing pharmaceutical products.

Sign in / Sign up

Export Citation Format

Share Document