Evaluation of the Antidiabetic and Antihyperlipidemic Activity of Spondias purpurea Seeds in a Diabetic Zebrafish Model

Diabetes mellitus (DM) is a serious chronic degenerative disease characterized by high levels of glucose in the blood. It is associated with an absolute or relative deficiency in the production and/or action of insulin. Some of the complications associated with DM are heart disease, retinopathy, kidney disease, and neuropathy; therefore, new natural alternatives are being sought to control the disease. In this work, we evaluate the antidiabetic effect of Spondias purpurea seed methanol extract (CSM) in vitro and in a glucose-induced diabetic zebrafish model. CSM is capable of lowering blood glucose and cholesterol levels, as well as forming advanced glycation end-products, while not presenting toxic effects at the concentrations evaluated. These data show that CSM has a promising antidiabetic effect and may be useful in reducing some of the pathologies associated with diabetes mellitus.

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Advanced glycation end products induce a prothrombotic phenotype in mice via interaction with platelet CD36

Blood ◽

10.1182/blood-2011-10-387506 ◽

2012 ◽

Vol 119 (25) ◽

pp. 6136-6144 ◽

Cited By ~ 62

Author(s):

Weifei Zhu ◽

Wei Li ◽

Roy L. Silverstein

Keyword(s):

Diabetes Mellitus ◽

Mouse Models ◽

Advanced Glycation End Products ◽

Vascular Complications ◽

Dependent Manner ◽

Advanced Glycation ◽

Drug Induced ◽

Glycation End Products ◽

End Products

Abstract Diabetes mellitus has been associated with platelet hyperreactivity, which plays a central role in the hyperglycemia-related prothrombotic phenotype. The mechanisms responsible for this phenomenon are not established. In the present study, we investigated the role of CD36, a class-B scavenger receptor, in this process. Using both in vitro and in vivo mouse models, we demonstrated direct and specific interactions of platelet CD36 with advanced glycation end products (AGEs) generated under hyperglycemic conditions. AGEs bound to platelet CD36 in a specific and dose-dependent manner, and binding was inhibited by the high-affinity CD36 ligand NO2LDL. Cd36-null platelets did not bind AGE. Using diet- and drug-induced mouse models of diabetes, we have shown that cd36-null mice had a delayed time to the formation of occlusive thrombi compared with wild-type (WT) in a FeCl3-induced carotid artery injury model. Cd36-null mice had a similar level of hyperglycemia and a similar level of plasma AGEs compared with WT mice under this condition, but WT mice had more AGEs incorporated into thrombi. Mechanistic studies revealed that CD36-dependent JNK2 activation is involved in this prothrombotic pathway. Therefore, the results of the present study couple vascular complications in diabetes mellitus with AGE-CD36–mediated platelet signaling and hyperreactivity.

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Experimental Animal Studies Support the Role of Dietary Advanced Glycation End Products in Health and Disease

Nutrients ◽

10.3390/nu13103467 ◽

2021 ◽

Vol 13 (10) ◽

pp. 3467

Author(s):

Melpomeni Peppa ◽

Ioanna Mavroeidi

Keyword(s):

Diabetes Mellitus ◽

Experimental Animal ◽

Advanced Glycation End Products ◽

Animal Studies ◽

Advanced Glycation ◽

Glycation End Products ◽

End Products ◽

Health And Disease

The increased incidence of obesity, diabetes mellitus, aging, and associated comorbidities indicates the interplay between genetic and environmental influences. Several dietary components have been identified to play a role in the pathogenesis of the so-called “modern diseases”, and their complications including advanced glycation end products (AGEs), which are generated during the food preparation and processing. Diet-derived advanced glycation end products (dAGEs) can be absorbed in the gastrointestinal system and contribute to the total body AGEs’ homeostasis, partially excreted in the urine, while a significant amount accumulates to various tissues. Various in vitro, in vivo, and clinical studies support that dAGEs play an important role in health and disease, in a similar way to those endogenously formed. Animal studies using wild type, as well as experimental, animal models have shown that dAGEs contribute significantly to the pathogenesis of various diseases and their complications, and are involved in the changes related to the aging process. In addition, they support that dAGEs’ restriction reduces insulin resistance, oxidative stress, and inflammation; restores immune alterations; and prevents or delays the progression of aging, obesity, diabetes mellitus, and their complications. These data can be extrapolated in humans and strongly support that dAGEs’ restriction should be considered as an alternative therapeutic intervention.

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In vitro Physicochemical, Phytochemical, Antimicrobial and Antidiabetic studies on Mucuna pruriens (Linn.) DC seeds

International Journal of Bioassays ◽

10.21746/ijbio.2016.06.0012 ◽

2016 ◽

Vol 5 (06) ◽

pp. 4650 ◽

Cited By ~ 1

Author(s):

Srilakshmi Kusuma* ◽

Aniel Kumar O. ◽

Lakshmi Narayana K. ◽

Sudhakar Pola ◽

Venkata Reddy K.

Keyword(s):

Developing Countries ◽

Methanol Extract ◽

Mucuna Pruriens ◽

Bioactive Properties ◽

Treatment Of Diabetes ◽

Relative Deficiency ◽

Alpha Glucosidase ◽

Crude Methanol Extract ◽

Seed Extracts

Diabetes is one of the most common endocrine diseases characterized by hyperglycemia due to absolute or relative deficiency of insulin which is currently affecting the citizens of both developed and developing countries. According to Williams textbook of endocrinology in 2013 it was estimated that over 382 million people throughout the world had diabetes. Plants have long been used for the treatment of diabetes, particularly in developing countries where most people have limited resources and do not have access to modern treatment. The presented study is aimed to evaluate the Mucuna pruriens seed extracts for its in vitro physiochemical, phytochemical, antioxidant and anti-diabetic studies. From the studies different solvent extracts of hexane and chloroform showed little or no activity on all assays performed whereas methanol extract of Mucuna pruriens showed significant bio properties. The preliminary studies of this plant crude methanol extract exhibited maximum compounds, hence the methanol extracts have under taken for its alpha amylase and alpha glucosidase inhibition activity. On the basis of the results obtained in the present investigation, it can be concluded that methanol extract of Mucuna pruriens had significant bioactive properties and may provide a support to use of the plant in traditional medicine for the management of diabetes.

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Advanced glycation end products affect cholesterol homeostasis by impairing ABCA1 expression on macrophages

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2017-0170 ◽

2017 ◽

Vol 95 (8) ◽

pp. 977-984 ◽

Cited By ~ 2

Author(s):

Olivier Kamtchueng Simo ◽

Souade Ikhlef ◽

Hicham Berrougui ◽

Abdelouahed Khalil

Keyword(s):

Advanced Glycation End Products ◽

Cholesterol Homeostasis ◽

High Density Lipoproteins ◽

Advanced Glycation ◽

Cholesterol Levels ◽

Glycation End Products ◽

End Products ◽

Prevention Of Atherosclerosis ◽

Abca1 Protein

Reverse cholesterol transport (RCT), which is intimately linked to high-density lipoproteins (HDLs), plays a key role in cholesterol homeostasis and the prevention of atherosclerosis. The goal of the present study was to investigate the effect of aging and advanced glycation end products (AGEs) on RCT as well as on other factors that may affect the antiatherogenic property of HDLs. The transfer of macrophage-derived cholesterol to the plasma and liver and then to the feces for elimination was significantly lower in aged mice than in young mice. Chronic injection of d -galactose (D-gal) or AGEs also significantly reduced RCT (65.3% reduction in [3H]cholesterol levels in the plasma of D-gal-treated mice after 48 h compared with control mice, P < 0.01). The injection of both D-gal and aminoguanidine hydrochloride increased [3H]cholesterol levels in the plasma, although the levels were lower than those of control mice. The in vitro incubation of HDLs with dicarbonyl compounds increased the carbonyl and conjugated diene content of HDLs and significantly reduced PON1 paraoxonase activity (87.4% lower than control HDLs, P < 0.0001). Treating J774A.1 macrophages with glycated fetal bovine serum increased carbonyl formation (39.5% increase, P < 0.003) and reduced ABCA1 protein expression and the capacity of macrophages to liberate cholesterol (69.1% decrease, P < 0.0001). Our results showed, for the first time, that RCT is altered with aging and that AGEs contribute significantly to this alteration.

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Antioxidant Activity and In Vitro Antiglycation of the Fruit of Spondias purpurea

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/5613704 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Alethia Muñiz ◽

Efren Garcia ◽

Daphne Gonzalez ◽

Lizette Zuñiga

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Antioxidant Activity ◽

Protein Carbonyl ◽

Oxidation Reactions ◽

Protein Carbonyl Content ◽

Life Threatening ◽

Glucose System ◽

Spondias Purpurea

Hyperglycemia in diabetes mellitus causes irreversible life-threatening micro- and macrovascular complications. There is evidence that the glycation reaction leads to a chemical modification of the proteins contributing to the complications of diabetes. It is known that advanced glycation end products (AGEs) are formed by glycation and oxidation reactions called glycoxidation. CML, a nonfluorescent AGE, has become a biomarker of glycoxidative damage; other AGEs appear to induce oxidative stress, which results in cytotoxicity. To determine antioxidant activity, the FRAP, DPPH, and TEAC tests were used, as well as the polyphenols content using Folin-Ciocalteu’s method. To evaluate the antiglycation activity, the BSA/glucose system was used, and the fructosamine concentration, protein carbonyl content, thiol, and CML groups were determined. The results obtained show that the hexane extract of the fruit of Spondias purpurea (CFH) effectively inhibits the glycation reaction, in addition to increasing the thiol groups and decreasing levels of fructosamine, protein carbonyl, and CML. In addition, CFH presented significant antioxidant activity. CFH inhibits the glycation reaction; therefore, it can help prevent complications related to AGEs in diabetes mellitus; it also reduces oxidative stress and is effective in protecting proteins from oxidative damage.

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Evaluation of Various Biological Activities of Endemic Sideritis libanotica Extracts

Notulae Scientia Biologicae ◽

10.15835/nsb11210442 ◽

2019 ◽

Vol 11 (2) ◽

pp. 210-217

Author(s):

Mehmet ATAS ◽

Nuraniye ERUYGUR ◽

Fazli SOZMEN ◽

Mustafa ERGUL ◽

Merve ERGUL ◽

...

Keyword(s):

Diabetes Mellitus ◽

Inhibitory Activity ◽

Methanol Extract ◽

Biological Activities ◽

Water Extract ◽

In Vitro Cytotoxicity ◽

Definitive Treatment ◽

Inhibition Activity ◽

Better Than

In recent years, using of plants to develop combine therapies for treatment of certain diseases such as cancer, Alzheimer's disease, and diabetes mellitus is extremely plausible approach. Perhaps, the definitive treatment of some of these diseases is hidden in the complex content of a plant in nature. Thus, in the present study, we decided to determine the biological activities of methanol and water extracts of the endemic Sideritis libanotica. To the best of our knowledge, the present study is the first investigation on antioxidant, enzyme inhibitory activity, antimicrobial activity, and in vitro cytotoxicity of S. libanotica. According to results, while the methanol extract shows better anti-cholinesterase, α-glucosidase, and α-amylase inhibition activity and cytotoxicity than the water extract, the tyrosinase inhibitory activity of the water extract was found to be better than the methanol extract. This study provides valuable information on how the biological activity of endemic S. libanotica changes in different solvent extractions such as water and methanol.

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The effect of Milgamma NR and SperminR on in vitro cellular immune reactions in patients with type II diabetes mellitus

Immunology Letters ◽

10.1016/s0165-2478(97)88807-4 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 482

Author(s):

M Horváth

Keyword(s):

Diabetes Mellitus ◽

Type Ii Diabetes ◽

Type Ii Diabetes Mellitus ◽

Type Ii ◽

Immune Reactions ◽

Cellular Immune

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Hydrolyzable tannins from the cores of Cornus officinalis with inhibitory activity in vitro on the formation of advanced glycation end products (AGEs)

Planta Medica ◽

10.1055/s-0028-1084383 ◽

2008 ◽

Vol 74 (09) ◽

Author(s):

GY Lee ◽

DS Jang ◽

JL Yoo ◽

YM Lee ◽

YS Kim ◽

...

Keyword(s):

Advanced Glycation End Products ◽

Inhibitory Activity ◽

Advanced Glycation ◽

Hydrolyzable Tannins ◽

Cornus Officinalis ◽

Glycation End Products ◽

End Products

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Apoptosis and proliferation ofperipheral blood T-cells as alternative processes in pathogenesis of diabetes mellitus type 1

Medical alphabet ◽

10.33667/2078-5631-2019-1-4(379)-16-20 ◽

2019 ◽

Vol 1 (4) ◽

pp. 16-20 ◽

Cited By ~ 1

Author(s):

A. V. Lugovaya ◽

N. M. Kalinina ◽

V. Ph. Mitreikin ◽

Yu. P. Kovaltchuk ◽

A. V. Artyomova ◽

...

Keyword(s):

Diabetes Mellitus ◽

T Cells ◽

High Risk ◽

Peripheral Blood ◽

Cellular Response ◽

Proliferative Potential ◽

Autoreactive T Cells ◽

Alternative Processes

Apoptosis, along with proliferation, is a form of lymphocyte response to activating stimuli. In the early stages of cell differentiation, the apoptotic response prevails and it results to the formation of tolerance to inductor antigen. Mature lymphocytes proliferate in response to stimulation and it means the initial stage in the development of the immune response. Since in this case apoptosis and proliferation acts as alternative processes, their ratio can serve as a measure of the effectiveness of the cellular response to activating signals. The resistance of autoreactive T-cells to apoptosis is the main key point in the development of type 1 diabetes mellitus (T1DM). Autoreactive T-cells migrates from the bloodstream to the islet tissue of the pancreas and take an active part in b cells destruction. The resistance of autoreactive effector T-cells to apoptosis may suggest their high proliferative potential. Therefore, the comparative evaluation of apoptosis and proliferation of peripheral blood lymphocytes can give a more complete picture of their functional state and thus will help to reveal the causes of ineffective peripheral blood T-ceiis apoptosis in patients with T1DM and will help to understand more deeply the pathogenesis of the disease. in this article, the features of proliferative response of peripheral blood T-cells in patients with T1DM and in individuals with high risk of developing T1DM have been studied. Apoptosis of T-cell subpopulations has been investigated. The correlation between the apoptotic markers and the intensity of spontaneous and activation- induced in vitro T-cells proliferation of was revealed. it was determined, that autoreactive peripheral blood T-cells were resistant to apoptosis and demonstrated the increased proliferative potential in patients with T1DM and in individuals with high risk of developing T1DM.

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