scholarly journals Nutritional Composition and Health Benefits of Various Botanical Types of Melon (Cucumis melo L.)

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1755
Author(s):  
Shivapriya Manchali ◽  
Kotamballi N. Chidambara Murthy ◽  
Vishnuvardana ◽  
Bhimanagouda S. Patil
Keyword(s):  
Cucumis Melo ◽  
Radical Scavenging Activity ◽  
Crop Improvement ◽  
Health Benefits ◽  
Radical Scavenging ◽  
Healthy Diet ◽  
Soluble Solids ◽  
In Vitro Assays ◽  
Potential Health ◽  
Cucumis Melo L

Characterizing the diverse melon cultivars for nutrition aids in crop improvement and promoting a healthy diet. Here, we used in vitro assays to characterize the nutritional qualities and health-beneficial effects of 30 melon (Cucumis melo L.) genotypes, including 10 improved cultivars, 16 landraces, and 4 wild types collected from different parts of India. Two landraces (Sidoota and Alper Green) had the highest (12.20 and 11.25) total soluble solids (TSS) contents. The Sidoota and Pappusa landraces had high reducing sugar contents (2.84 and 2.81 mg g−1 fresh weight [FW]). The highest polyphenols contents (22.0 mg g−1 FW) were observed in the landraces Mage Kaayi-2, Budamekaayi, and Small Melon. Reflecting on the primary and secondary metabolite contents, the Mekke Kaayi and Giriyala landraces exhibited high 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (97.6 and 91% at 100 μg mL−1). Additionally, seven of the landraces showed significant nitric oxide (NO) induction activity (>80% inhibition at 200 μg mL−1), indicating their potential health benefits, and seven showed considerable angiotensin-converting enzyme (ACE) inhibition activity (highest in Kashi Madhu), indicating their potential usefulness in reducing hypertension. Genotypes with high health beneficial compounds identified in this study can be used for breeding improved melon cultivars to promote these fruits as well as a healthy diet.

scholarly journals Optimization of Extraction Solvent and Fast Blue BB Assay for Comparative Analysis of Antioxidant Phenolics from Cucumis melo L.

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1379
Author(s):  
Varsha Ravindranath ◽  
Jashbir Singh ◽  
Guddarangavvanahally K. Jayaprakasha ◽  
Bhimanagouda S. Patil
Keyword(s):  
Phenolic Compounds ◽  
Cucumis Melo ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Standard Samples ◽  
Methanol Extracts ◽  
Fast Blue ◽  
Cucumis Melo L

Melon (Cucumis melo L.) fruits contain multiple health-promoting compounds, including phenolic compounds, which are antioxidants. Accurate measurement of antioxidant activities and total phenolic contents (TPCs) require an efficient solvent extraction. In this study, we evaluated free radical scavenging activity and TPC of melon extracts extracted with 22 different solvent combinations. The DPPH scavenging activities were high in 100% methanolic (39.48 ± 0.36 µg g−1) and 80% methanolic extracts (38.99 ± 0.44 µg g−1). Similarly, the ABTS scavenging activities were high in 100% methanolic (315.11 ± 10.38 µg g−1) and 80% methanol extracts (297.39 ± 14.98 µg g−1). The Folin–Ciocalteu (F–C) assay is typically used to measure TPC but may be affected by interference from sugars and other compounds. Therefore, we optimized an assay for TPC using Fast Blue (FB) salt and developed a standard operating procedure for microplate analysis using FB. Our analysis of standard samples and comparisons with the F–C assay suggested that the optimized FB assay could be used to measure TPC in fruit and juice samples. Moreover, we successfully detected six phenolic compounds in methanol extracts of melon by LC-HR-QTOF/MS.

scholarly journals Oenothera laciniata Hill Extracts Exhibits Antioxidant Effects and Attenuates Melanogenesis in B16-F10 Cells via Downregulating CREB/MITF/Tyrosinase and Upregulating p-ERK and p-JNK

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 727
Author(s):  
Horng-Huey Ko ◽  
Yeo-Tzu Chang ◽  
Yueh-Hsiung Kuo ◽  
Chia-Hsuan Lin ◽  
Yih-Fung Chen
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Tyrosinase Activity ◽  
Perennial Herb ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Protective Agent

Oenothera laciniata Hill is a perennial herb traditionally used to alleviate inflammatory complications. This study investigated the antioxidant and anti-melanogenic activities of O. laciniata. The methanolic extract (OLM) of O. laciniata and its different fractions, including ethyl acetate (OLEF), n-butanol (OLBF), and water (OLWF) fractions, were prepared. Antioxidant activities were evaluated by total phenolic content, the radical-scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+•), and superoxide anion (O2−•), reducing capacity, and metal chelating ability. OLM and its fractions exhibited potent antioxidant activity in these in vitro assays, with a correlation between radical-scavenging activity and total phenolic content. OLM and its fractions inhibited the mushroom tyrosinase activity superior to the reference control, ascorbic acid. In B16-F10 melanoma cells, OLM and its fractions significantly decreased melanin production and tyrosinase activity. Mechanistic investigations revealed that OLM and its fractions inhibited tyrosinase and TRP-2 expressions via downregulating MITF and phosphorylated CREB and differentially inducing ERK or JNK phosphorylation. Additionally, OLM and its fractions caused no significant cytotoxicity towards B16-F10 or skin fibroblast cells at concentrations used in these cellular assays. These findings demonstrated the potential of O. laciniata extracts as the ideal skin protective agent with dual antioxidant and anti-melanogenic activities.

scholarly journals A Comparative Study of the Antioxidative Effects of Helichrysum italicum and Helichrysum arenarium Infusions

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 380
Author(s):  
Katja Kramberger ◽  
Zala Jenko Pražnikar ◽  
Alenka Baruca Arbeiter ◽  
Ana Petelin ◽  
Dunja Bandelj ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Morphological Type ◽  
Mass Spectrometry Analysis ◽  
In Vitro Assays ◽  
Spectrometry Analysis ◽  
Stress Related Genes

Helichrysum arenarium (L.) Moench (abbrev. as HA) has a long tradition in European ethnomedicine and its inflorescences are approved as a herbal medicinal product. In the Mediterranean part of Europe, Helichrysum italicum (Roth) G. Don (abbrev. as HI) is more common. Since infusions from both plants are traditionally used, we aimed to compare their antioxidative potential using in vitro assays. Two morphologically distinct HI plants, HIa and HIb, were compared to a commercially available HA product. Genetic analysis using microsatellites confirmed a clear differentiation between HI and HA and suggested that HIb was a hybrid resulting from spontaneous hybridization from unknown HI subspecies. High-performance liquid chromatography–mass spectrometry analysis showed the highest amounts of hydroxycinnamic acids and total arzanol derivatives in HIa, whereas HIb was richest in monohydroxybenzoic acids, caffeic acids, and coumarins, and HA contained the highest amounts of flavonoids, especially flavanones. HIa exhibited the highest radical scavenging activity; it was more efficient in protecting different cell lines from induced oxidative stress and in inducing oxidative stress-related genes superoxide dismutase 1, catalase, and glutathione reductase 1. The antioxidative potential of HI was not only dependent on the morphological type of the plant but also on the harvest date, revealing important information for obtaining the best possible product. Considering the superior properties of HI compared to HA, the evaluation of HI as a medicinal plant could be recommended.

scholarly journals Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots

2020 ◽  
Vol 10 (3) ◽  
pp. 133-136
Author(s):  
Ahlem Karbab ◽  
Kamel Mokhnache ◽  
Lekhmici Arrar ◽  
Abderahmane Baghiani ◽  
Seddik Khennouf ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Health Benefit ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Potential Health

The purpose of this study was to prepare three sub-fractions from Pituranthos scoparius roots (PSR), characterize their phytochemicals contents and to investigate their free radical scavenging activity by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl scavenging activities. Tannins, flavonoids, steroids, and other bioactive compounds were found in the different sub-fractions. The Ethyle acetate extract (EAE) and chloroform extract (ChE) exhibited the highest antioxidant activity using ABTS (17.8 ± 0.87 µg/mL and 18.15 ± 0.68 µg/mL), respectively. Whereas, Crude extract (CrE) have been presented strong hydroxyl scavenging activity (14.9 ± 0.8 µg/mL). This study indicates that PSR extracts has potent free radical scavenging, and may prove to be of potential health benefit as well as additional resources for natural antioxidants. Keywords: Medicinal plant; phytochemical screening; sub-fractions, free radical scavenging.

scholarly journals Phytochemical analysis, Antimicrobial, insecticidal and antiradical activity of Hydnocarpus pentandra (Buch.-Ham.) Oken

2017 ◽  
Vol 9 (4) ◽  
pp. 576
Author(s):  
Prashith Kekuda TR ◽  
Dunkana Negussa Kenie ◽  
Chetan DM ◽  
Raghavendra L Hallur
Keyword(s):  
Leaf Extract ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Process ◽  
In Vitro Assays ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Bioactive Agents

<p><strong>Objectives</strong>: The present study was conducted to evaluate antimicrobial, insecticidal and radical scavenging activity of leaf extract of <em>Hydnocarpus pentandra</em> (Buch.-Ham.) Oken belonging to the family Achariaceae.</p><p><strong>Methods</strong>: Extraction process of shade dried and powdered leaf was carried out by maceration technique. Extract was screened for phytochemicals by standard tests. Antibacterial and antifungal activity of leaf extract was determined by Agar well diffusion and Poisoned food technique respectively. Antiradical activity of leaf extract was evaluated by two in vitro assays namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays. Insecticidal activity of leaf extract was determined against II instar and IV instar larvae of <em>Aedes aegypti</em>.</p><p><strong>Results</strong>: Preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins, saponins, glycosides, triterpenes and steroids in the leaf extract. Leaf extract exhibited marked inhibitory activity against Gram positive bacteria when compared to Gram negative bacteria. <em>Bacillus cereus</em> (zone of inhibition 1.86±0.05cm) and <em>Escherichia coli</em> (zone of inhibition 1.06±0.05cm) were inhibited to highest and least extent respectively. Extract was effective in inhibiting mycelial growth of seed-borne fungi. Among fungi, the susceptibility to extract was in the order: <em>Curvularia</em> sp. (53.64% inhibition) &gt; <em>Fusarium</em> sp. (45.81% inhibition) &gt; <em>Alternaria</em> sp. (35.08% inhibition). The extract exhibited concentration dependent larvicidal activity with marked activity being observed against II instar larvae (LC<sub>50</sub> value 0.79mg/ml) when compared to IV instar larvae (LC<sub>50</sub> value 1.37mg/ml). Leaf extract scavenged DPPH and ABTS radicals dose dependently with an IC<sub>50</sub> value of 13.91µg/ml and 6.03µg/ml respectively.</p><p><strong>Conclusions</strong>: The plant is shown to be an important source of bioactive agents. The observed bioactivities could be attributed to the phytochemicals present in the leaf extract. Further studies on characterization and bioactivity determination of isolated components from leaf extract are to be carried out.</p>

Potential antioxidant and lipid peroxidation inhibition of coffee mixed with lemongrass (Cymbopogon citrates) leaves

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Ayman El-Anany ◽  
Sami Althwab ◽  
Rehab Ali ◽  
Rehab F.M. Ali ◽  
Hassan Mousa
Keyword(s):  
Lipid Peroxidation ◽  
Health Benefits ◽  
Radical Scavenging ◽  
Control Sample ◽  
Reducing Power ◽  
Storage Period ◽  
Dpph Radical ◽  
Potential Health ◽  
Content Type ◽  
Different Levels

Purpose The purpose of this study is to evaluate the effect of the addition of dried lemongrass leaves (DLGL) powder, at different levels, on phenolics content, antioxidant activities, consumer acceptance and the inhibition of lipid peroxidation of roasted coffee (RC). Design/methodology/approach DLGL powder was incorporated at the levels of 0%, 2.5%, 5.0%, 7.5% and 10% of RC weight. The total flavonoids (TF), total phenolics (TP) and antioxidant activity measured using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reducing power assay of RC, DLGL and binary mixture of them determined. The oxidative indices of coffee oil samples during storage were investigated. In addition, the sensory characteristics of RC fortified with different levels of DLGL powder were evaluated. Findings The TP content of DLGL powder was 1,100.32 mg/100 g DWb, nearly 1.2 times higher than found in RC beans. The TF content of RC enriched with 2.5%, 5.0%, 7.5% and 10% DLGL were found to be around 1.05, 1.10, 1.15 and 1.20 times higher than that in the control coffee samples. RC supplemented with various levels of DLGL powder showed higher DPPH radical scavenging and reducing power activities. At the end of the storage period (six months), the acid, peroxide, P-Anisidine and total oxidation value values of RC supplemented with 10% DLGL powder were about 1.94, 2.52, 2.60 and 2.59 times as low as in the control sample without any addition of DLGL powder, respectively. RC containing 2.5% and 5.0% DLGL powder had significantly (p < 0.05) the highest sensory scores. Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits. Practical implications RC containing 2.5% and 5.0% DLGL powder had significantly (p = 0.05) the highest sensory scores. Originality/value Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits.

scholarly journals Behaviour of 9-Ethyl-9H-carbazole Hydrazone Derivatives Against Oxidant Systems

2019 ◽  
Vol 92 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Cigdem Karaaslan ◽  
Hande Gurer-Orhan ◽  
Sibel Suzen ◽  
Luciano Saso ◽  
Omidreza Firuzi ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Amyloid Β ◽  
In Vitro Assays ◽  
Cytotoxic Activities ◽  
Scavenging Activity ◽  
Neuroprotective Effects ◽  
Neuronal Pc12 Cells

Antioxidants are helpful in prevention of several diseases related with oxidative stress including neurodegenerative disorders. In recent studies, carbazoles were given proof of promising antioxidant activities. In this article, 9-ethyl-9H-carbazole hydrazone derivatives were synthesized, characterized and their in vitro antioxidant activity and possible cytotoxic effects were investigated. Furthermore, protective effect of the synthesized derivatives against amyloid β-induced damage in PC12 neuronal cells was examined by using MTT assay. The newly synthesized carbazoles were found to have radical scavenging activity with a varying potency both in cell-free and cell-based in vitro assays. Several compounds, especially such as 3d and 3e, 3m and 3n bearing two halogen groups on the phenyl ring, were found to have cytotoxic activity. However, their cytotoxic activities were not higher than that of melatonin. Several compounds also significantly protected neuronal PC12 cells against amyloid β-induced damage, which can be defined as neuroprotective agents. (4-(2-((9-Ethyl-9H-carbazol-3-yl)methylene)hydrazinyl)benzonitrile) 3r was found as the most active compound with both radical scavenging activity and neuroprotective effects against amyloid β-induced damage. These findings might provide an alternative strategy for developing novel carbazole derivatives for management of neurodegenerative diseases, such as Alzheimer's disease.

scholarly journals The Influence Of Cultivation System On Biochemical Content Of Strawberry Fruits

2014 ◽  
Vol 22 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Valda Laugale ◽  
Sarmite Strautina ◽  
Inta Krasnova ◽  
Dalija Seglina ◽  
Kaspars Kampuss
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Content ◽  
Titratable Acidity ◽  
Climatic Conditions ◽  
Soluble Solids ◽  
Chemical Content ◽  
Black Plastic ◽  
Low Tunnels

AbstractThree cultivars ‘Zefyr’, ‘Honoeoye’ and ‘Polka’ were grown on beds mulched with black plastic or without it, as well as in low tunnels covered by transparent polyethylene film or Agronet (Pegas agro, 17 g·m-2), or without covering. The quality of fruits determined by content of ascorbic acid, titratable acidity, soluble solids, total content of phenolics and anthocyanins, antiradical activity, and radical scavenging activity (RSA) was evaluated during two seasons. Significant differences in phytochemical contents within strawberry cultivars, production seasons and cultivation methods were stated. Cultivar had the greatest influence on the content of ascorbic acid, total phenolics and RSA among all the studied factors. The amount of anthocyanins was mainly influenced by plant covering, while the annual climatic conditions had the greatest impact on the amount of titratable acidity and soluble solids. Soil mulching had the lowest influence on the chemical content of fruits.

scholarly journals Cultivar effect on the sweet cherry antioxidant and some chemical attributes

2016 ◽  
Vol 28 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Jan Skrzyński ◽  
Małgorzata Leja ◽  
Aleksander Gonkiewicz ◽  
Przemysław Banach
Keyword(s):  
Organic Acids ◽  
Sweet Cherry ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Chemical Attributes ◽  
Sweet Cherry Cultivars

Abstract This research was carried out to evaluate the phenolic composition, antioxidant capacities, sugars and organic acids content of sweet cherry cultivars (Bianca, Burlat, Johanna 1, Johanna 2, Kordia, Kunzes Kirsche, Merton Premier, Napoleon, Orleans, Regina, Rivan, Schneiders Spate Knorpelkirsche, Summit, Trebnitzer Lotkirsche) grown in Poland. Significant differences were observed between tested cultivars for all studied parameters. The sum of total soluble solids ranged from 14.3 (‘Burlat’) to 20.9 g 100 g−1 FW (‘Bianca’) and that of organic acids from 0.43 (‘Burlat’) to 0.76 g 100 g−1 FW (‘Napoleon’). Fruit of Bianca cultivar showed the highest levels of anthocyanin (108.5 mg 100 g−1 FW) while ‘Napoleon’ and ‘Kunzes Kirsche’ contained the lowest levels (1.5 and 1.8 mg 100 g−1 FW, respectively). Total phenolic contents ranged from 101 (‘Napoleon’) to 558 (‘Bianca’) mg 100 g−1 FW, tartaric esters from 26.2 (‘Summit’) to 66.5 (‘Bianca’) mg 100 g−1 FW and flavonoids from 7.9 (‘Summit’) to 49.1 (‘Bianca’) mg 100 g−1 FW. Bianca cultivar has also the highest free radical scavenging activity assayed by ABTS and DPPH methods 88 and 90% respectively.

scholarly journals In Vitro Antidiabetic Effects and Antioxidant Potential ofCassia nemophilaPods

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Gauhar Rehman ◽  
Muhammad Hamayun ◽  
Amjad Iqbal ◽  
Saif Ul Islam ◽  
Saba Arshad ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Yeast Cells ◽  
In Vitro Assays ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

The antidiabetic and antioxidant potential of ethanolic extract ofCassia nemophilapod (EECNP) was evaluated by three in vitro assays, including yeast glucose uptake assay, glucose adsorption assay, and DPPH radical scavenging activity. The result revealed that the extracts have enhanced the uptake of glucose through the plasma membrane of yeast cells. A linear increase in glucose uptake by yeast cells was noticed with gradual increase in the concentration of the test samples. Moreover, the adsorption capacity of the EECNP was directly proportional to the molar concentration of glucose. Also, the DPPH radical scavenging capacity of the extract was increased to a maximum value of 43.3% at 80 μg/ml, which was then decreased to 41.9% at 100 μg/ml. From the results, it was concluded that EECNP possess good antidiabetic and antioxidant properties as shown by in vitro assays.

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