Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity, and Thermal Stability of Rubus matsumuranus Phenolome

Rubus matsumuranus H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific information on R. matsumuranus leaves contributed to the investigation of the metabolomic profile and biological activity of this plant. In this study, metabolites of R. matsumuranus leaves in three stages (active growth, flowering, and fruiting) were characterised using high-performance liquid chromatography with photodiode array and electrospray ionisation triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS). In total, 63 compounds were identified, including gallic acid derivatives, hydroxycinnamates, catechins, procyanidins, flavonols, and ellagitannins. Lambertianin C (57.11 mg/g of dry weight, DW), miquelianin (39.63 mg/g DW), and kaempferol-3-O-glucuronide (31.18 mg/g DW) were the major compounds in R. matsumuranus leaves. As a result of the HPLC-PDA-based assay to determine the antioxidant activity, it was revealed that lambertianin A, sanguiin H6, lambertianin C, and sanguiin H11 were effective scavengers of free radicals (2,2-diphenyl-1-picrylhydrazyl, DPPH•) and possessed Fe2+-chelating activity. After an investigation of the phenolic content in infusions and decoctions obtained by extraction with water at different temperatures, it was revealed that a hot infusion (80 °C) is a phenolic-rich preparation of R. matsumuranus leaves. Our research suggests that R. matsumuranus leaves are a rich source of phenolic compounds with high antioxidant properties and that this could be a prospective plant for new functional products.

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Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage

Molecules ◽

10.3390/molecules23092216 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2216 ◽

Cited By ~ 7

Author(s):

Cai-Ning Zhao ◽

Guo-Yi Tang ◽

Qing Liu ◽

Xiao-Yu Xu ◽

Shi-Yu Cao ◽

...

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

High Performance ◽

Antioxidant Properties ◽

Dry Weight ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Herbal Tea ◽

Antioxidant Power

The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe2+/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and p-coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.

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Analysis of phenolic profile, total phenolic content and antioxidant activity in Anacardium occidentale leaves

Food Research ◽

10.26656/fr.2017.6(1).105 ◽

2022 ◽

Vol 6 (1) ◽

pp. 20-26

Author(s):

A. Sassi ◽

Normah H. ◽

M.M.A.K. Khattak ◽

Hanapi M.J.

Keyword(s):

Type 2 Diabetes ◽

Antioxidant Activity ◽

High Performance Liquid Chromatography ◽

Gallic Acid ◽

High Performance ◽

Sinapinic Acid ◽

Dry Weight ◽

Photodiode Array ◽

Phenolic Extract

Anacardium occidentale young leaves are consumed traditionally as part of a Southeast Asian diet. The regular consumption is believed to have beneficial effects on health in general and potentially against type 2 Diabetes mellitus due to its high content of polyphenols. This study was aimed to investigate the polyphenol content of the plant using two methanol extracts; Free Phenolic Extract (FPE) and Bound Phenolic Extract (BPE) as well as highlight the presence of six phenolic acids and flavonoids namely; gallic acid, sinapinic acid, caffeic acid, ferulic acid, quercetin and kaempferol using High performance liquid chromatography-photodiode array (HPLC-UV-Vis). The results for polyphenols and flavonoids content showed high amounts of total polyphenols in BPE with 8.5±0.57 mg GAE/g as well as high amounts of total flavonoids in both extracts FPE and BPE with 0.58±0.06 and 0.86±0.05 mg QE/g respectively (p<0.05). The presence of these polyphenols was further confirmed by measuring the antioxidant activity through the scavenging of the free radical DPPH which showed an IC50 value for FPE (5.17±0.64 µg/ mL, BPE (4.96±0.12 µg/mL) compared to the positive control ascorbic acid (4.91±0.43 µg/mL). The high-performance liquid chromatography-photodiode array confirmed the presence of all four targeted phenolic acids with the highest amount showing in gallic acid and sinapinic acid in BPE with 148.12±6.44 µg gallic acid/g dry weight and 47.02±1.94 µg sinapinic acid/g dry weight respectively. As for flavonoids, quercetin was present in both extracts with 20.38±1.22 µg/g dry weight in BPE and 5.21±0.1 µg/g dry weight in FPE while Kaempferol was not detectable in either extract. These findings confirmed the importance of A. occidentale as a rich source of polyphenols which can be further investigated to determine its effects in-vitro and in-vivo on non-communicable diseases such as type 2 diabetes.

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PHYTOCHEMICAL AND ANTIOXIDANT COMPOSITION IN ACCESSIONS OF THE MANGABA ACTIVE GERMPLASM BANK

Revista Caatinga ◽

10.1590/1983-21252021v34n123rc ◽

2021 ◽

Vol 34 (1) ◽

pp. 228-235

Author(s):

PRISCILLA SANTANA SANTOS ◽

LISIANE DOS SANTOS FREITAS ◽

EVANDRO NEVES MUNIZ ◽

JOSEFA GRASIELA SILVA SANTANA ◽

ANA VERUSKA CRUZ DA SILVA

Keyword(s):

Principal Component Analysis ◽

Antioxidant Activity ◽

High Performance Liquid Chromatography ◽

High Performance ◽

Antioxidant Properties ◽

Principal Component ◽

Total Phenols ◽

Germplasm Bank ◽

Fruit Species ◽

Hancornia Speciosa

ABSTRACT Mangaba (Hancornia speciosa Gomes) is a fruit species native to Brazil, belonging to the Apocynaceae family. Its cultivation is predominantly extractivist and its antioxidant properties have been recently reported. The objective of this study was to evaluate the bioactive compounds and antioxidant activity in fruits of accession from the Active Germplasm Bank of Embrapa Coastal Tablelands (BI, CA, LG, PR, PT and TC). The extracts were analyzed by high-performance liquid chromatography (HPLC), which identified chlorogenic acid (93.71 - 131.66 mg.100g-1), ferulic acid (0.85 - 2.27 mg.100g-1) and rutin (238.59 -442.94 mg.100g-1). The accessions CA and PR showed the highest values of total phenols (1179.39 and 1167.05 mg GAE.100g-1, respectively). The accessions CA and TC had the highest concentration of the major compound rutin (436.78 and 442.94 mg.100g-1, respectively). Antioxidant activity values ranged from 125.95 to 158.67 g.g-1 DPPH. Principal component analysis grouped the accessions into four groups, due to the genetic variability verified in previous studies. The results will be useful to guide actions of selection and future breeding program of the species.

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Biocomponents and Antioxidant Activity of Ribes khorasanicum

International journal of basic science in medicine ◽

10.15171/ijbsm.2018.18 ◽

2018 ◽

Vol 3 (3) ◽

pp. 99-103 ◽

Cited By ~ 7

Author(s):

Mohammad Ehsan Taghavizadeh Yazdi ◽

Jalil Khara ◽

Mohammad Reza Housaindokht ◽

Hamid Reza Sadeghnia ◽

Sedigheh Esmaeilzadeh Bahabadid ◽

...

Keyword(s):

Antioxidant Activity ◽

Sugar Content ◽

Antioxidant Properties ◽

Dry Weight ◽

Total Phenolic ◽

Anthocyanin Content ◽

Bioactive Components ◽

Total Anthocyanin ◽

Total Anthocyanin Content ◽

Biological And Medical Applications

Currently, there have been lots of interests in phytochemicals as bioactive components. The roles of fruit, vegetables, and red pigments in preventing diseases have been partly accredited to the antioxidant properties of their constituent polyphenols, flavonoid, anthocyanins, and etc. Biochemistry parameters including the relative levels of antioxidant activity, total phenolic content, total flavonoid, total anthocyanin, soluble and insoluble sugar content of Ribes khorasanicum, have been calculated in this project. The total anthocyanin content of the investigated Ribes khorasanicum measured to be 62.9 mg/g in dry weight, while displaying high levels when compared to other components, which indicates that the anthocyanin content was the predominant antioxidant components in the investigated plant. This particular plant has the potential to serve as the important source of antioxidant that can be utilized in different biological and medical applications.

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The Effect of Heating Temperature on Total Phenolic Content, Antioxidant Activity, and Phenolic Compounds of Plum and Mahaleb Fruits

International Journal of Food Engineering ◽

10.1515/ijfe-2017-0302 ◽

2019 ◽

Vol 15 (11-12) ◽

Cited By ~ 2

Author(s):

Kashif Ghafoor ◽

Isam A. Mohamed Ahmed ◽

Süleyman Doğu ◽

Nurhan Uslu ◽

Gbemisola J. Fadimu ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Gallic Acid ◽

Total Phenolic Content ◽

Phenolic Content ◽

Heating Temperature ◽

Dry Weight ◽

Total Phenolic ◽

Dihydroxybenzoic Acid ◽

Different Temperatures

AbstractThe effect of heating at different temperatures (60, 80, 90, 110, and 130 °C) on the total phenolic content, antioxidant activity, and phenolic compounds present in plum and mahaleb fruits was investigated. The antioxidant activity values and total phenolic contents of fresh plum (93.82% measured by DPPH method, 787.79 mg gallic acid equivalent (GAE)/100 g dry weight determined by Folin method) and mahaleb fruits (81.80%, 634.47 mg GAE/100 g dry weight) were higher than plum and mahaleb fruits dried at different temperatures (p < 0.05). Generally, the heating process caused a reduction in both total phenolic content and antioxidant activity for plum and mahaleb. While (+)-catechin (92.62 mg/kg), 1,2-dihydroxybenzene (132.15 mg/kg), gallic acid (107.01 mg/kg), and 3,4-dihydroxybenzoic acid (74.59 mg/kg) are the key phenolic compounds in fresh plum, (+)-catechin, 3,4-dihydroxybenzoic acid, 1,2-dihydroxybenzene, and syringic and caffeic acids were the major phenolic compounds of mahaleb fruits. The polyphenol content of fruits and the class of phenolics present are significantly affected by heating temperature.

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Studies of the Variability of Polyphenols and Carotenoids in Different Methods Fermented Organic Leaves of Willowherb (Chamerion angustifolium (L.) Holub)

Applied Sciences ◽

10.3390/app10155254 ◽

2020 ◽

Vol 10 (15) ◽

pp. 5254

Author(s):

Marius Lasinskas ◽

Elvyra Jariene ◽

Nijole Vaitkeviciene ◽

Jurgita Kulaitiene ◽

Katarzyna Najman ◽

...

Keyword(s):

Antioxidant Activity ◽

High Performance ◽

Solid Phase ◽

Antioxidant Properties ◽

Hierarchical Cluster ◽

Organic Production ◽

Biologically Active ◽

Total Phenolic ◽

Total Carotenoids ◽

Chamerion Angustifolium

The demand for organic production is increasing worldwide. The willowherb, grown in an organic way, contributes greatly to the idea of a healthier society and clean land. Willowherb is widespread in the world and has high polyphenols, carotenoids, and antioxidant properties. The purpose of this work was to investigate the influence of solid-phase fermentation (SPF) under different conditions on the variation of polyphenols and carotenoids in the organic leaves of willowherb. The leaves were fermented for different periods of time: 24, 48, and 72 h; and in aerobic and anaerobic conditions. The evaluation of polyphenols and carotenoids was completed using high-performance liquid chromatography (HPLC), and antioxidant activity was measured with spectrophotometric method. Hierarchical cluster analysis was used to describe differences in biologically active compounds between willowherb samples. The experiment showed that the highest quantities of total phenolic acids and flavonoids were determined after 24 h under aerobic SPF, but the amountof total carotenoids was higher after 72 h anaerobic SPF, compared to control. Not-fermented willowherb leaves had a lower antioxidant activity. compared to fermented leaves. In conclusion, SPF can be used to change polyphenol and carotenoid quantities in organic leaves of willowherb.

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Determination of Phenolic Composition and Antioxidant Activity in Flowers and Stems of Rumex Tunetanus Using RP-UHPLC-ESI-QTOF-MS

10.20944/preprints201812.0027.v1 ◽

2018 ◽

Author(s):

Jouda Abidi ◽

Sonda Ammar ◽

Mohamed Bouaziz

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

Folk Medicine ◽

Antioxidant Properties ◽

Bioactive Compound ◽

Selective Extraction ◽

Total Phenolic ◽

Negative Ionization Mode ◽

Negative Ionization

The present study was designed to investigate the bioactive compound in Rumextunetanus extracts (polygonaceae), a plant growing in GarâaSejnane region (NW-Tunisia). Hydro-methanol extracts of flowers and stems of Rumextunetanus were analyzed by RP-UHPLC-ESI-QTOF-MS in the negative mode to identify the maximum of bioactive compounds. Applied the aforementioned method, a total of 60 bioactive compounds were characterized for the first time in Rumextunetanus between them, 18 photochemical were firstly identified in the Polygonaceae family in negative ionization mode. Quantification of the identified compounds revealed that quercetin-3-O-glucuronide and (-)-epicatechingallate were the most abundant phenolic compounds in flowers and stems, respectively. Moreover, positive correlations were found between the antioxidant activity measured by DPPH and FRAP assays with the total phenolic compounds (r = 0.98; r = 0.99, respectively) and the abundance of some phenolic subfamilies such as hydroxycinnamic acids, hydroxybenzoic acids, flavonols and flavones with r > 0.86. The compounds displaying significant (P < 0.01) and good correlations with the antioxidant activity (r > 0.93) were hydroxybenzoic acid, rutin, quercetin-3-O-glucuronide, quercetin-3-O-glucoside, quercetin and luteolin-7-O-rutinoside. In addition, the flowers and stems of Rumex tunetanus showed different bioactive compound profiles and significant antioxidant properties of extracts. These results highlight the potential of the RP-UHPLC-ESI-QTOF-MS and MS/MS system to identify untargeted metabolic profiling of Rumex tunetanus. Overall, these results contribute to the clear explanation of the past and current usage of genus Rumex in folk medicine. Future investigations are necessary to develop purified antioxidant extracts, with the application of more selective extraction techniques.

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Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

F1000Research ◽

10.12688/f1000research.22380.1 ◽

2020 ◽

Vol 9 ◽

pp. 220

Author(s):

Fitmawati Fitmawati ◽

Esi Resida ◽

Sri Nur Kholifah ◽

Rodesia Mustika Roza ◽

Muhammad Almurdani ◽

...

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

High Performance ◽

Dry Weight ◽

Hplc Method ◽

Flavonoid Compound ◽

The Public ◽

New Findings ◽

Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.2270-35.4763 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.47 mg/g dry weight with the lowest value in M. foetida2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

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Variations in Anthocyanin Profiles and Antioxidant Activity of 12 Genotypes of Mulberry (Morus spp.) Fruits and Their Changes during Processing

Antioxidants ◽

10.3390/antiox9030242 ◽

2020 ◽

Vol 9 (3) ◽

pp. 242 ◽

Cited By ~ 3

Author(s):

Inhwan Kim ◽

Jihyun Lee

Keyword(s):

Antioxidant Activity ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Citric Acid ◽

High Performance ◽

Antioxidant Activities ◽

Dry Weight ◽

Mulberry Fruit ◽

Mulberry Fruits ◽

Anthocyanin Composition

Mulberry fruits are known as rich sources of anthocyanins and are consumed in syrup form after the addition of sugar and acid; however, there is little information on the anthocyanin composition and antioxidant activity of mulberries of different cultivars and their changes during processing. To address this, the antioxidant activity and anthocyanin composition of 12 cultivar mulberry fruit cultivars were investigated by high-performance liquid chromatography and ultra-high-performance liquid chromatography coupled with electrospray ionization/quadrupole time-of-flight. Additionally, different quantities of citric acid were used to evaluate antioxidant activities and anthocyanin composition of mulberry syrup. Sixteen anthocyanins were identified in mulberry fruits using accurate mass spectrometry. Several anthocyanins were tentatively identified for the first time in mulberry fruits and include: malvidin hexoside, cyanidin malonyl hexose hexoside, cyanidin pentoside, cyanidin malonyl hexoside, petunidin deoxyhexose hexoside, and cyanidin deoxyhexoside. The major anthocyanin in mulberries was cyanidin-3-O-glucoside, followed by cyanidin-3-O-rutinoside. Morus Alba L. Iksu showed the highest cyanidin-3-O-glucoside content (8.65 mg/g dry weight) among 12 mulberry fruit cultivars. As citric acid levels increased, mulberry syrup showed significantly higher antioxidant activity (p < 0.05).

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Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models

Molecules ◽

10.3390/molecules25215149 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5149

Author(s):

Roberto O. Ybañez-Julca ◽

Daniel Asunción-Alvarez ◽

Ivan M. Quispe-Díaz ◽

Javier Palacios ◽

Jorge Bórquez ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Protective Effect ◽

High Performance ◽

Mangifera Indica ◽

Folk Medicine ◽

In Vitro Assays ◽

Antispasmodic Activity ◽

Intestinal Segments

Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.

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