scholarly journals Dental Sealant Empowered by 1,3,5-Tri Acryloyl Hexahydro-1,3,5-Triazine and α-Tricalcium Phosphate for Anti-Caries Application

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 895 ◽  
Author(s):  
Juliana Caletti Monteiro ◽  
Michele Stürmer ◽  
Isadora Martini Garcia ◽  
Mary Anne Melo ◽  
Salvatore Sauro ◽  
...  
Keyword(s):  
Contact Angle ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Tricalcium Phosphate ◽  
Calcium Phosphates ◽  
Dental Materials ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Knoop Hardness ◽  
Dental Sealant

: Quaternary ammonium compounds and calcium phosphates have been incorporated into dental materials to enhance their biointeractivity and preventive effects. This study aimed at evaluating the physical and chemical properties and effects against Streptococcus mutans of a dental sealant containing 1,3,5-tri acryloyl hexahydro-1,3,5-triazine (TAT) and α-tricalcium phosphate (α‐TCP). A methacrylate-based dental sealant was initially formulated. α‐TCP and TAT (Gα-TCPTAT) were added to the experimental sealant at 2 wt.% each. One group was formulated without α‐TCP and TAT and used as control (GCTRL). All tested resins were analyzed for polymerization kinetics and degree of conversion (DC %), Knoop hardness (KHN), softening in solvent (∆KHN%), ultimate tensile strength (UTS), the contact angle with water or with α-bromonaphthalene, surface free energy (SFE) and antibacterial activity against Streptococcus mutans in biofilm and in planktonic cells. The polymerization kinetic was different between groups, but without statistical differences in the DC % (p<0.05). KHN and ΔKHN% did not change between groups (p>0.05), but Gα-TCPTAT presented greater UTS compared to GCTRL (p<0.05). No differences were found for contact angle (p>0.05) or SFE (p>0.05). Gα-TCPTAT showed greater antibacterial activity in comparison to GCTRL (p<0.05). The formulation of dental sealants containing TAT and α-TCP can be characterized by improved mechanical and antibacterial properties.

scholarly journals Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on Streptococcus mutans

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 532 ◽  
Author(s):  
Barbara Lapinska ◽  
Magdalena Konieczka ◽  
Beata Zarzycka ◽  
Krzysztof Sokolowski ◽  
Janina Grzegorczyk ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Dental Materials ◽  
Antibacterial Properties ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Bonding Systems ◽  
Self Etch

There is no consensus on the antibacterial activity of dentin bonding systems (DBS). Many study models have been used to evaluate the antimicrobial activity of dental materials. In this study, a novel detection method, flow cytometry, was introduced. It allows for evaluation of the antibacterial activity of DBS, based on assessment of the disruption of the bacterial physical membrane induced by DBS. The aim of the study was to evaluate the antibacterial properties of selected dentin bonding systems against Streptococcus mutans. The highest antibacterial activity against S. mutans was observed for Adhese Universal (99.68% dead cells) and was comparable to that of Prime&Bond Universal, OptiBond Universal, or Clearfil Universal Bond Quick (p > 0.05). The lowest activity of all tested systems was displayed by the multi-mode adhesive, Universal Bond (12.68% dead bacteria cells), followed by the self-etch adhesive, OptiBond FL (15.58% dead bacteria cells). The present study showed that in the case of two-component DBS, the primer exhibited higher antimicrobial activity than the adhesive (or bond) itself.

scholarly journals Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3854
Author(s):  
Joanna Czechowska ◽  
Ewelina Cichoń ◽  
Anna Belcarz ◽  
Anna Ślósarczyk ◽  
Aneta Zima
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Tricalcium Phosphate ◽  
Setting Time ◽  
Antibacterial Properties ◽  
Bone Substitutes ◽  
Bacterial Strains ◽  
Setting Times ◽  
Biological Studies

Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies.

scholarly journals Minireview: Formulasi Obat Kumur Ekstrak Daun Ketul (Bidens pilosa)

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Zidane Irwansyah ◽  
Hadinata Santoso ◽  
Mariana Wahjudi
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Cardiac Glycosides ◽  
Antibacterial Properties ◽  
Tween 80 ◽  
Peppermint Oil ◽  
Literature Study ◽  
Bidens Pilosa ◽  
Extraction Solvent ◽  
Water Viscosity

Abstract— Ketul (Bidens pilosa L) is weed plant that has antibacterial properties. The potential of this plant has not been developed yet in Indonesia. We conducted a literature study about this plant use for herbal mouthwash. The purposes of this study were to determine components of Ketul leaf with antibacterial activity, to know the extraction solvent, to determine the method for antibacterial activity, and to predict the characteristics and components of herbal mouthwash formula. The results showed that Ketul leaf contains tannins, cardiac glycosides, saponins, alkaloids, flavonoids, and terpenoids which could inhibit the growth of various bacteria, including Streptococcus mutans. Hence the mouthwash is predicted to have antibacterial activity. The safe extraction solvent widely used was 70-80% ethanol. The Ketul leaf extract has the potential to be applied in herbal mouthwash formula. Other characteristic can be adjusted to meet the requirements as herbal mouthwash, which are pH between 5-7, the viscosity value near water viscosity value (1cP), stable during storage, and color is brownish yellow. Some common additives were added in mouthwash to improve the flavors and taste, such as sorbitol, tween-80, peppermint oil, and sodium benzoate. Keywords: antibacterial activity, solvent, Streptococcus mutans, weed Abstrak— Ketul (Bidens pilosa L) merupakan tumbuhan gulma yang memiliki aktivitas antibakteri. Potensi tumbuhan ini belum banyak dikembangkan di Indonesia. Pada tulisan ini telah dilakukan kajian pustaka pemanfaatan Ketul untuk obat kumur herbal. Tujuan kajian ini adalah untuk mengetahui komponen ekstrak daun Ketul yang berkhasiat antibakteri, mengetahui pelarut ekstraksi, metode penentuan aktivitas antibakteri obat kumur, dan prediksi karakteristik dan komponen obat kumur herbal daun Ketul. Hasil penelusuran pustaka menunjukkan bahwa daun Ketul mengandung tannin, glikosida jantung, saponin, alkaloid, flavonoid, dan terpenoid, yang dapat menghambat pertumbuhan berbagai bakteri, termasuk Streptococcus mutans sehingga obat kumur kemungkinan memiliki aktivitas antibakteri. Pelarut aman yang banyak digunakan untuk ekstraksi komponen fitokimia tersebut adalah etanol 70-80%. Penentuan daya antibakteri dapat dilakukan menggunakan metode difusi agar atau pengenceran. Ekstrak daun Ketul berpotensi digunakan dalam formula obat kumur herbal. Selain potensi antibakterinya, karakteristik lainnya dapat diatur untuk memenuhi syarat formula obat kumur herbal yaitu pH berkisar 5-7, nilai viskositas mendekati nilai viskositas air (1cP), stabilitas dapat tetap terjaga selama penyimpanan dan kemungkinan obat kumur berwarna kuning kecoklatan. Beberapa bahan tambahan, seperti sorbitol, tween-80, peppermint oil, dan natrium benzoate, merupakan bahan tambahan yang aman dan umum digunakan untuk menambah cita rasa dan stabilitas formula obat kumur herbal ekstrak daun Ketul. Kata kunci: aktivitas antibakteri, gulma, pelarut, Streptococcus mutans

scholarly journals ANALISIS BIOAUTOGRAFI DAN KARAKTERISASI DENGAN FTIR PADA FRAKSI DAUN LABU SIAM (Sechium edule (jacq).SW) TERHADAP Porphyromonas gingivalis DAN Streptococcus mutans

1970 ◽  
pp. 55-66
Author(s):  
Laila Susanti ◽  
Isbiyantoro Isbiyantoro ◽  
Septiana Simanjuntak
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Bacterial Suspension ◽  
Chloroform Fraction ◽  
Contact Method ◽  
Fractionation Process ◽  
Sechium Edule

Siamese pumpkin leaves are one of the plants that have antibacterial properties. Compounds that act as antibacterial are flavonoite, tannin, triterpenes, alkaloids, and saponins. The aim of this study was to prove the antibacterial activity of ethanol hexane and chloroform fraction of pumpkin siam leaves and characterize the functional groups of active compounds from pumpkin siam leaves (Sechium edule (jacq). SW) with FTIR spectrophotometers. The extraction process of Siamese Pumpkin leaves was carried out by maceration method using 70% ethanol. The extract was continued with the fractionation process with ethanol, n-hexane and chloroform solvents. Antibacterial activity testing using the well method with a concentration of 100%, positive control chlorhexidi and negative control aquadst. The compound content test used the Thin Layer Chromatography (TLC) method with the stationary phase using silica plate G60 F254 and comparison of the mobile phase of chloroform: methanol: water (2: 5: 3) (v / v). Bioautography test using the contact method, namely the elution TLC plate, was placed on the NA medium containing a bacterial suspension for 3 hours. Antibacterial test results The largest ethanol fraction of pumpkin leaves was at a concentration of 100% with a diameter of inhibition zone of 11.49 mm on P. gingivalis while 16.04 mm in S. mutans. The results of TLC showed the presence of flavonoids with a price of Rf 0.47 and tannin with a price of Rf 0.84. Bioautography results showed a inhibition zone with a price of Rf 0.49 in the bacteria P. gingivalis and S. mutans which are flavonoid compounds. Characterization by FTIR spectrophotometer O-H phenol, C = C aromatic, C-O-C ether, aromatic C-H functional group Keywords: (Sechium edule (jacq) .SW). Porphyromonas Gingivalis, Streptococcus mutans, TLC, Bioautography and FTIR

scholarly journals Antibacterial effects of soft denture reline resin containing natural extract

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 446-449
Author(s):  
Yu-Ri Choi ◽  
Min-Kyung Kang
Keyword(s):  
Contact Angle ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Confocal Laser ◽  
Control Group ◽  
Denture Base ◽  
Significant Difference ◽  
Base Resin ◽  
Denture Base Resin ◽  
Experimental Group

Candida albicans (C. Albicans) is one of the bacteria that reside in the oral cavity, and the ones living in medical and commercial denture resins, commonly cause diseases. Therefore, this study was conducted to confirm the antibacterial activity of C. Albicans using a denture base resin containing peony extract with antibacterial properties. Contact angle measurements and color change measurements were performed to confirm the physical change of the material added with the antibacterial agent to the denture reline resin. As a result of the antibacterial test, the experimental group exhibited antibacterial activity against C. Albicans. Compared to the optical density results, the results of the experimental group showed a significant difference. As a result of Fluorescent images showing (confocal laser microscope), the control group showed a lot of live bacteria, and no bacteria appeared in the experimental group. All group  did not show any physical changes. As a result of the contact angle measurement, the surface of the experimental group was changed to hydrophilic. In addition, there was no change in the color of the denture reline resin containing peony extract. In conclusion, it was confirmed that the peony extract contained antibacterial activity of the denture resin, and further studies should be conducted on various bacteria for denture base resin disinfection.

scholarly journals Nanosilver Application in Dental Cements

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ana Paula Rodrigues Magalhães ◽  
Laura Barbosa Santos ◽  
Lawrence Gonzaga Lopes ◽  
Cyntia Rodrigues de Araújo Estrela ◽  
Carlos Estrela ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Statistical Analysis ◽  
Streptococcus Mutans ◽  
Bactericidal Activity ◽  
Agar Plate ◽  
Antibacterial Properties ◽  
Tooth Decay ◽  
Control Groups ◽  
Made In

Streptococcus mutans is the microorganism mostly responsible for initiation of tooth decay and also for the progression of an established lesion. Silver has been used for its antibacterial properties for many years, in different forms: ionised and elementary forms, as silver zeolites or as nanoparticles. The purpose of this study was to evaluate the antibacterial activity of three dental cements modified by nanosilver. Three cements were used: Sealapex, RelyX ARC, and Vitrebond. The cements were incorporated with 0.05 mL of silver nanoparticles solution. Control groups were prepared without silver. Six Petri plates with BHI were inoculated with S. mutans using sterile swabs. Three cavities were made in each agar plate (total = 18) and filled with the manipulated cements. They were incubated at 37°C for 48 h, and the inhibition halos were measured. The paired t-Test was used for statistical analysis (P<0.05). No inhibition halos were obtained for Sealapex and Rely X, but Vitrebond showed bactericidal activity without silver and enhanced effect with silver incorporation.

Antibacterial Effect of Experimental Orthodontic Adhesives Containing Gold Nanoparticles against Streptococcus mutans and Streptococcus sobrinus

2021 ◽  
Vol 904 ◽  
pp. 301-308
Author(s):  
Pinyada Akarajarasrod ◽  
Surachai Dechkunakorn ◽  
Pornpen Tantivitayakul ◽  
Primana Punnakitikashem ◽  
Wassana Wichai ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Bacterial Growth ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Control Group ◽  
White Spot Lesion ◽  
Streptococcus Sobrinus ◽  
Orthodontic Adhesives

Enamel decalcification usually can be found around orthodontic bracket after debonding. Orthodontic adhesives resistant to bacterial colonization were used to prevent white spot lesion and dental caries in orthodontic treatment with fixed appliance. The objectives of this study were to evaluate the antibacterial properties of expeimental orthodontic adhesive containing gold nanoparticles (AuNPs). 108 orthodontic adhesive discs containing 1.0 wt%, 0.5 wt%, 0.25 wt% AuNPs and without AuNPs were prepared from in-house orthodontic adhesive (27 discs per group). The antibcterial properties of adhesive discs were evaluated by direct contact test. Streptococcus mutans and Streptococcus sobrinus suspensions were placed on the discs and incubated at 37 °C for 1 hour. The adhesive discs were transferred to BHI broth and were incubated at 37°C for 16, 20 and 24 h. Bacterial growth was evaluated by optical density (OD) measurement at 590nm. The results showed that experimental orthodontic adhesives with 1.0 wt% and 0.5 wt% AuNPs exhibited higher antibacterial activity compared to the control group (p < 0.05). At 24 h of incubation, the median OD of 1.0 wt%, 0.5 wt%, 0.25 wt% and control in S. mutans were 0.109, 0.006, 0.007 and 0.372, rescpectively. In S. sobrinus, the median OD were 0.173, 0.012 , 0.007 and 0.328. The incorporation of gold nanoparticles into orthodontic adhesives exhibited antibacterial activity against cariogenic microorganism. At a concentration of 0.5 wt% and 1.0 wt% AuNPs, experimental orthodontic adhesive could reduce bacterial growth of both S. mutans and S. sobrinus.

scholarly journals Phytochemical Characterisation and Antibacterial Activity of Propolis on Cariogenic Microrganisms

2020 ◽  
pp. 56-66
Author(s):  
Ashu Michael Agbor ◽  
Raissa Tsague ◽  
Bruno Kaptue ◽  
Sudeshni Naidoo ◽  
Estella Tembe-Fokunang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Aqueous Extract ◽  
Antibacterial Properties ◽  
Hydroalcoholic Extract ◽  
Chemical Screening ◽  
Dental Tissues ◽  
Lactobacillus Spp

Background: Dental caries is also known as tooth decay is as a result of the softening of hard dental tissues from factors of multiple origins, with dental plaque bacteria being one of the most important factors. Due to the resistance of microorganisms to common antibiotics, modern medical sciences had been looking for new approaches in the treatment and prevention of oral diseases.  Objective: This study aimed to phytochemically characterise, evaluate the antibacterial activity of propolis on bacteria responsible for dental caries. Methodology: This was an experimental study that took place in the Laboratory of Chemistry and Microbiology (Clinque Universities des Montagne (CUM)) of Bangangté between January to April 2018. Chemical screening of the aqueous and hydroethanolic extracts revealed the presence of  secondary metabolites namely alkaloids, flavonoids, tannins, coumarins, saponins and terpenes, those being more abundant in the aqueous extract. The content of flavonoids was high. Macro-dilution was used to determine the minimum inhibitory concentrations (MIC) of propolis and  Müller Hinton agar was used for obtaining the minimum bactericidal concentrations (MBC) and determination of inhibition diameters of the bacteria. Results: The flavonoid content in the aqueous extract was estimated at 222.85 mg/ml and 18.77 mg/ml for the hydroalcoholic extracts. S mutans and Lactobacillus spp. were isolated from samples collected from caries affected teeth. The aqueous extract of propolis generally generates greater inhibition diameters than that of the hydroalcoholic extract on both lactobacillus and Streptococcus mutans, The MBC of the aqueous extract on S. mutans and Lactobacillus spp. was 50 mg/ml and the MIC was 25 mg/ml. The hydroalcoholic extract did not have MBC on both S. mutans and Lactobacillus spp. However, the MIC of this extract on both bacteria was 50 mg/ml. These antibacterial properties were not statically significant as that of gentamicin. Conclusion: The evaluation of the antibacterial activity of our various extracts by determination of the MIC and MBC revealed that propolis was endowed with inhibitory properties vis-à-vis the growth of most bacterial strains tested: Streptococcus mutans and Lactobacillus spp. all 2 being gram-positive bacteria.

scholarly journals Preparation of nanocomposite silver/kaolin with antibacterial properties

2021 ◽  
Vol 340 ◽  
pp. 01030
Author(s):  
Balnur Baltabayeva ◽  
Aliya Ospanova ◽  
Zhanar Kubasheva
Keyword(s):  
Antibacterial Activity ◽  
Chemical Reduction ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Silica Matrix ◽  
Gram Positive Bacteria ◽  
Composition And Structure ◽  
Bet Method ◽  
Mobile Ions ◽  
Physical And Chemical

In this study, a nanocomposite based on kaolin from the Alekseevsky deposit (Kazakhstan), containing nano-silver particles, was synthesized. Kaolin has good physical and chemical properties and is an effective carrier for medicines. The synthesis of AgNPs/kaolin nanocomposites was carried out by chemical reduction. Impregnation of silver nanoparticles into silica matrix can prevent the agglomeration of mobile ions, and thereby preserve the antibacterial activity of the compound. The composition and structure of the resulting nanocomposite were studied using SEM/EDS analysis to study the morphology and elemental composition of natural and silver-modified kaolin, the specific surface was determined by the BET method, and functional groups were identified by IR spectroscopy. The resulting composite was tested for antibacterial activity against Gram-negative and Gram-positive bacteria (E.Сoli, S. Aureus).

scholarly journals The Toxicity and Antibacterial Properties of Calamus ornatus Bl. Rattan Fruit

2019 ◽  
Vol 39 (4) ◽  
pp. 350
Author(s):  
Heriad Daud Salusu ◽  
Ernita Obeth ◽  
Abdul Rasyid Zarta ◽  
Eva Nurmarini ◽  
Hamka Nurkaya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Traditional Medicine ◽  
Streptococcus Mutans ◽  
Brine Shrimp ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Low Toxicity ◽  
Fruit Pericarp ◽  
Toxicity Level

Calamus ornatus Bl., locally known as rotan mantang or rotan seuti is one of the commercial rattan species growing in the forests of East Borneo. Its fruit is edible, like the fruits of other species of rattan, and it has been used by the local people as traditional medicine to treat stomachache or toothache. Until recently, there has not been any study investigating the benefits of the fruit for medicinal or other purposes. This research aimed to discover the bio-activity potentials of Calamus ornatus Bl. fruits by identifying its toxicity level and antibacterial potentials. The parts of the fruit (pericarp, flesh, and seed) were extracted using ethanol as solvent to extract samples. The toxicity was analyzed by using the Brine Shrimp Lethality Test (BSLT) method while the antibacterial activity was tested using a well diffusion method. Low toxicity was found in the flesh, whereas the pericarp and the seed were non-toxic. Antibacterial activity against Escherichia coli was found in the pericarp and the seed, while antibacterial activity against Streptococcus mutans was found in the pericarp and the flesh.

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