scholarly journals Prediction of the Biogenic Amines Index of Poultry Meat Using an Electronic Nose

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1580 ◽  
Author(s):  
Wojciech Wojnowski ◽  
Kaja Kalinowska ◽  
Tomasz Majchrzak ◽  
Justyna Płotka-Wasylka ◽  
Jacek Namieśnik

The biogenic amines index of fresh chicken meat samples during refrigerated storage was predicted based on the headspace analysis using an electronic nose equipped with an array of electrochemical sensors. The reference biogenic amines index values were obtained using dispersive liquid–liquid microextraction–gas chromatography–mass spectrometry. A prototype electronic nose with modular construction and a dedicated sample chamber was used to rapidly analyze the volatile fraction of chicken meat samples, with a single measurement time of five minutes. Back-propagation artificial neural network was used to estimate the biogenic amines index of the samples with a determination coefficient of 0.954 based on ten-fold stratified cross-validation. The results indicate that the determination of the biogenic amines index is a good reference method for studies in which the freshness of meat products is assessed based on headspace analysis and fingerprinting, and that the described electronic device can be used to assess poultry meat freshness based on this value with high accuracy.

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Sylvio Barbon ◽  
Ana Paula Ayub da Costa Barbon ◽  
Rafael Gomes Mantovani ◽  
Douglas Fernandes Barbin

Identification of chicken quality parameters is often inconsistent, time-consuming, and laborious. Near-infrared (NIR) spectroscopy has been used as a powerful tool for food quality assessment. However, the near-infrared (NIR) spectra comprise a large number of redundant information. Determining wavelengths relevance and selecting subsets for classification and prediction models are mandatory for the development of multispectral systems. A combination of both attribute and wavelength selection for NIR spectral information of chicken meat samples was investigated. Decision Trees and Decision Table predictors exploit these optimal wavelengths for classification tasks according to different quality grades of poultry meat. The proposed methodology was conducted with a support vector machine algorithm (SVM) to compare the precision of the proposed model. Experiments were performed on NIR spectral information (1050 wavelengths), colour (CIEL∗a∗b∗, chroma, and hue), water holding capacity (WHC), and pH of each sample analyzed. Results show that the best method was the REPTree based on 12 wavelengths, allowing for classification of poultry samples according to quality grades with 77.2% precision. The selected wavelengths could lead to potential simple multispectral acquisition devices.


2021 ◽  
Vol 15 (2) ◽  
pp. 121-126
Author(s):  
Majid Gholami-Ahangaran ◽  
◽  
Asiye Ahmadi-Dastgerdi ◽  
Shahrzad Azizi ◽  
◽  
...  

Background: One of the concerns of consumers of animal protein sources is the residues of heavy metals in meat. The accumulation of these elements in meat and the consumption by humans can cause acute and chronic disorders in the function of vital organs. Methods: For the estimation of cadmium, zinc and silver in the poultry meat products, 100 samples of chicken breasts, thighs, hearts and livers were collected from meat markets in Isfahan Province. The tissues were digested and passed through filter paper, and the cadmium, zinc and silver contents were measured by an atomic absorption spectrophotometer. Results: The mean contents of cadmium in the chicken thigh, breast, liver and heart samples were 0.055±0.021, 0.048±0.023, 0.074±0.031, and 0.012±0.004 mg/kg, respectively. The mean contents of zinc in the chicken thigh, breast, liver and heart samples were 17.19±8.10, 15.70±5.75, 22.10±10.87, and 19.70±10.38 mg/kg, respectively. The concentrations of cadmium and zinc were below the international permissible limits in all samples. The mean concentrations of silver in some chicken thigh, breast, liver and heart samples were 0.0080±0.0044, 0.0048±0.0013, 0.012±0.009 and 0.0036±0.006, respectively. Of note, silver was not detected in most chicken samples. Conclusion: This study did not find cadmium, zinc and silver in the chicken meat samples above the international permissible limits. However, frequent monitoring for heavy metals in poultry meat sold in public markets is warranted to prevent their transmission to the human food chain.


2020 ◽  
Vol 13 (3) ◽  
pp. 440-445
Author(s):  
Amal Awad ◽  
Mayada Gwida ◽  
Eman Khalifa ◽  
Asmaa Sadat

Aim: The present study was designed to investigate the occurrence and distribution of Salmonella serotypes in chicken meat samples, and to explore the susceptibility of the strains to antimicrobials, as well as their virulence-associated genes. Materials and Methods: Two-hundred retail chicken meat samples from different shops, as well as 25 stool specimens from retail shop workers, were included in the study. The collected samples were examined bacteriologically for the presence of salmonellae. Salmonella isolates were serotyped using a slide agglutination test for O and H antigens and were screened for the presence of five virulence genes (stn, pef, invA, sopB, and avrA) using a uniplex polymerase chain reaction assay and for their susceptibility to 18 antimicrobial agents using the disk diffusion method. Results: Thirty-one Salmonella isolates belonging to 12 different serovars were identified. Salmonella Enteritidis and Salmonella Kentucky were the dominant serovars (22.6% each). Salmonella isolates displayed a high antibiotic resistance against erythromycin, sulfamethoxazole/trimethoprim, doxycycline, cephalexin, cefaclor, tetracycline, polymyxin B, cefuroxime, vancomycin, and streptomycin. All Salmonella isolates exhibited multidrug resistance (MDR) and demonstrated different virulence genes. The majority of Salmonella serovars (87.1%) harbored sopB gene, 54.8% carried avrA and pef genes, while all isolates carried invA and stn genes. Conclusion: The presence of virulent MDR Salmonellae in raw chicken meat could allow the possibility of transmission of these resistant serovars to humans. Therefore, strict hygienic measures should be followed on the whole poultry production chain to decrease the potential transmission of Salmonella infection from poultry meat to humans.


Author(s):  
Sumedha Bobade ◽  
K. Vijayarani ◽  
K.G. Tirumurugaan ◽  
A. Thangavelu ◽  
S. Vairamuthu

Background: Campylobacter species are a leading cause of most important food-borne diarrhoeal illness worldwide while, poultry has been identified as a significant cause of Campylobacter infection in humans. C. jejuni is highly effective in colonizing chicken intestinal mucosa without causing any clinical manifestations and the consumption of poultry meat is the major source of transmission of bacteria to humans. Methods: The total of 19 chicken meat samples collected from retail markets in Chennai were screened by cultural examination, further subjected to phenotypic characterization using biochemical test and genotypic characterization using polymerase chain reaction assay targeting hip O and map A genes. Result: All the isolates showed growth on modified blood free charcoal cefoperazone deoxycholate agar media (mCCDA) and 18 (94.73%) samples showed typical morphological characteristics. The 12 (63.15%) isolates showed biochemical reactions positive. The results from polymerase chain reaction showed that 10 (83.33%) isolates were positive for C. jejuni. This study suggested that, it is essential to investigate the incidence of Campylobacter jejuni infection in poultry and the risk factors at all production stages of meat production to help reducing the disease in humans in terms of food safety.


Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 264
Author(s):  
Iván D. Regalado-Pineda ◽  
Rene Rodarte-Medina ◽  
Carolina N. Resendiz-Nava ◽  
Cinthia E. Saenz-Garcia ◽  
Pilar Castañeda-Serrano ◽  
...  

Worldwide, chicken meat is considered one of the main sources of Salmonella enterica in humans. To protect consumers from this foodborne pathogen, international health authorities recommend the establishment of continuous Salmonella surveillance programs in meat. However, these programs are scarce in many world regions; thus, the goal of the present study was to perform a longitudinal surveillance of S. enterica in chicken meat in Mexico. A total of 1160 samples were collected and analyzed monthly from 2016 to 2018 in ten chicken meat retailers (supermarkets and wet markets) located in central Mexico. The isolation and identification of S. enterica was carried out using conventional and molecular methods. Overall, S. enterica was recovered from 18.1% (210/1160) of the chicken meat samples. Remarkably, during the three years of evaluation, S. enterica was more prevalent (p < 0.0001) in supermarkets (27.2%, 158/580) than in wet markets (9.0%, 52/580). The study was 3.8 times more likely (odds ratio = 3.8, p < 0.0001) to recover S. enterica from supermarkets than wet markets. Additionally, a higher prevalence (p < 0.05) of this pathogen was observed during the spring, summer, autumn, and winter in supermarkets compared with wet markets. Moreover, the recovery rate of S. enterica from supermarkets showed a gradual increase from 20.78% to 42% (p < 0.0001) from 2016 to 2018. Interestingly, no correlation (p > 0.05) was observed between the S. enterica recovery rate in chicken meat and reported cases of Salmonella infections in humans. Higher levels of S. enterica in chicken meat retailed in supermarkets are not unusual; this phenomenon has also been reported in some European and Asian countries. Together, these results uncover an important health threat that needs to be urgently addressed by poultry meat producers and retailers.


2014 ◽  
Vol 77 (9) ◽  
pp. 1579-1582 ◽  
Author(s):  
RAN-HEE YOON ◽  
SE-YEOUN CHA ◽  
BAI WEI ◽  
JAE-HEE ROH ◽  
HYE-SUK SEO ◽  
...  

Contamination of Salmonella was assessed in duck and chicken meat collected from supermarkets, traditional markets, internet shopping malls, and wholesale markets in Jeonlado, South Korea, in 2013. Salmonella contamination was found in 51.3% of duck meat samples and 3.7% of chicken meat samples. Salmonella contamination of duck meat samples differed by meat type, i.e., 69.8% of samples of whole ducks and 33.9% of samples of duck pieces. Six serotypes were identified from 64 Salmonella isolates in duck meat: Salmonella Typhimurium (37.5%), Salmonella Enteritidis (21.8%), Salmonella Stanley (3.1%), Salmonella Regent (1.6%), Salmonella Winterthur (3.1%), and Salmonella Westhampton (1.6%). All isolates were resistant to one or more antibiotics. Resistance to sulfisoxazole was most common (93.8% of isolates), followed by resistance to nalidixic acid (59.4%), ceftazidime (26.6%), and ampicillin (26.6%). To our knowledge, this study is the first to report Salmonella contamination in duck meat from Korea. Duck meat should be considered an important source of foodborne pathogens.


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