scholarly journals Biogenic Amines in Traditional Fiore Sardo PDO Sheep Cheese: Assessment, Validation and Application of an RP-HPLC-DAD-UV Method

Separations ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 11 ◽  
Author(s):  
Claudia Zazzu ◽  
Margherita Addis ◽  
Marco Caredda ◽  
Maria Francesca Scintu ◽  
Giovanni Piredda ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Total Concentration ◽  
Great Variability ◽  
Quantitative Distribution ◽  
Qualitative And Quantitative ◽  
Rp Hplc ◽  
Protected Designation Of Origin ◽  
Assessment Validation ◽  
The Many ◽  
First Time

This contribution aimed to measure for the first time the amount of biogenic amines (BAs) in one of the most ancient and traditional sheep cheese produced in Sardinia, Italy: the Protected Designation of Origin (PDO) Fiore Sardo. To achieve this, an original RP-HPLC-DAD-UV method has been developed that was completely validated in terms of LoD, LoQ, linearity, precision and trueness, and tested on 36 real Fiore Sardo PDO cheese samples produced by four different cheesemakers and marketed by four stores. The average total concentration of the eight BAs (i.e., tyramine, tryptamine, histidine, putrescine, cadaverine, 2-phenylethylamine, spermine and spermidine) measured in Fiore Sardo cheese was 700 mg/kg, with a range between 170 mg/kg and 1,100 mg/kg. A great variability in the total amount of BAs has been evidenced among the Fiore Sardo marketed in the four stores as well as for the cheeses purchased in different times in the same store. Tyramine (350 mg/kg), putrescine (150 mg/kg), histamine (80 mg/kg) and cadaverine (30 mg/kg) are the most abundant BAs found in this matrix. Among the many factors concurring, the dominant microflora of Fiore Sardo PDO is likely the principal cause of the qualitative and quantitative distribution of BAs in this matrix. Finally, the total amount of BAs found in Fiore Sardo PDO is not able to cause any health alert situation for consumers.

scholarly journals Biogenic Amines in Traditional Fiore Sardo PDO Sheep Cheese: Assessment, Validation and Application of a RP-HPLC-DAD-UV Method

2019 ◽  
Author(s):  
Claudia Zazzu ◽  
Margherita Addis ◽  
Marco Caredda ◽  
Maria Francesca Scintu ◽  
Giovanni Piredda ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Total Concentration ◽  
Great Variability ◽  
Quantitative Distribution ◽  
Qualitative And Quantitative ◽  
Rp Hplc ◽  
Protected Designation Of Origin ◽  
Assessment Validation ◽  
First Time

This contribution is aimed to measure for the first time the amount of biogenic amines (BAs) in one of the most ancient and traditional sheep cheese produced in Sardinia, Italy: the Protected Designation of Origin (PDO) Fiore Sardo. For doing this, an original RP-HPLC-DAD-UV method has been developed, completely validated in terms of LoD, LoQ, linearity, precision and trueness, and tested on 36 real Fiore Sardo PDO cheese samples produced by four different cheesemakers and marketed by four stores. The average total concentration of the eight BAs (i.e. tyramine, tryptamine, histidine, putrescine, cadaverine, 2-phenylethylamine, spermine and spermidine) measured in Fiore Sardo cheese was 700 mg/kg, with a range between 170 mg/kg and 1100 mg/kg. A great variability in the total amount of BAs has been evidenced among the Fiore Sardo marketed in the four stores as well as for the cheeses purchased in different times in the same store. Tyramine (350 mg/kg), putrescine (150 mg/kg), histamine (80 mg/kg) and cadaverine (30 mg/kg) are the most abundant BAs found in this matrix. Among many factors concurring, the dominant microflora of Fiore Sardo PDO is likely the principal cause of the qualitative and quantitative distribution of BAs in this matrix. Finally, the total amount of BAs found in Fiore Sardo PDO is not able to cause any situation of health alert for consumers.

scholarly journals New Analytical Tools for Unmasking Frauds in Raw Milk-Based Dairy Products: Assessment, Validation and Application to Fiore Sardo PDO Cheese of a RP-HPLC Method for the Evaluation of the α-l-Fucosidase Activity

Separations ◽  
2020 ◽  
Vol 7 (3) ◽  
pp. 40
Author(s):  
Alessio Silvio Dedola ◽  
Luca Piras ◽  
Margherita Addis ◽  
Antonio Pirisi ◽  
Giovanni Piredda ◽  
...  
Keyword(s):  
Raw Milk ◽  
Hplc Method ◽  
Lactation Period ◽  
Ripening Time ◽  
Rp Hplc ◽  
Protected Designation Of Origin ◽  
Assessment Validation ◽  
Raw Milk Cheese ◽  
Analytical Tools ◽  
First Time

The activity of α-l-fucosidase (FSC) has been measured for the first time in Fiore Sardo PDO (Protected Designation of Origin) raw milk cheese. To do this, a RP-HPLC method has been developed, validated and tested on a reliable sampling of cheese experimentally produced in laboratory batches. Three experimental factors have been considered in this work: the thermal treatment undergone by the milk, the lactation period and the ripening time of cheese. Results obtained have evidenced: (i) a meaningful reduction in the activity of FSC from cheeses produced using raw milk to those obtained by thermized milk; (ii) an increase in the activity of FSC during the first months of lactation period (from December to February), followed by a substantial constancy in the central and final months of lactation (from February to May); (iii) the enzyme activity is independent of the ripening time. This method might be useful in revealing frauds related to the use of mild thermal treatments of the milk when this is not allowed as for Fiore Sardo PDO cheese but also for several PDO cheeses for which the specifications establish that raw milk only must be used for their production.

Unrivalled Influence

2013 ◽  
Author(s):  
Judith Herrin
Keyword(s):  
Political Life ◽  
General Introduction ◽  
Women And Gender ◽  
Mothers And Daughters ◽  
Diverse Range ◽  
Long Span ◽  
Imperial Court ◽  
And Gender ◽  
The Many ◽  
First Time

This book explores the exceptional roles that women played in the vibrant cultural and political life of medieval Byzantium. This book evokes the complex and exotic world of Byzantium's women, from empresses and saints to uneducated rural widows. Drawing on a diverse range of sources, the book sheds light on the importance of marriage in imperial statecraft, the tense coexistence of empresses in the imperial court, and the critical relationships of mothers and daughters. It looks at women's interactions with eunuchs, the in-between gender in Byzantine society, and shows how women defended their rights to hold land. The book describes how women controlled their inheritances, participated in urban crowds demanding the dismissal of corrupt officials, followed the processions of holy icons and relics, and marked religious feasts with liturgical celebrations, market activity, and holiday pleasures. The vivid portraits that emerge here reveal how women exerted an unrivalled influence on the patriarchal society of Byzantium, and remained active participants in the many changes that occurred throughout the empire's millennial history. The book brings together the author's finest essays on women and gender written throughout the long span of her career. This volume includes three new essays published here for the very first time and a new general introduction. It also provides a concise introduction to each essay that describes how it came to be written and how it fits into her broader views about women and Byzantium.

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

Telluride and After

2021 ◽  
Vol 75 (2) ◽  
pp. 102-108
Author(s):  
B. Ruby Rich
Keyword(s):  
New York ◽  
Film Festival ◽  
Center Stage ◽  
Kenneth Branagh ◽  
Todd Haynes ◽  
Telluride Film ◽  
Velvet Underground ◽  
The Many ◽  
First Time

FQ editor-in-chief B. Ruby Rich reports from the 48th edition of the Telluride Film Festival. Unlike most of its peer festivals, Telluride opted not to hold a virtual edition in 2020, a decision entirely in keeping with its emphasis on the tactile and experiential aspects of cinema, and which made its return in 2021 all the more giddy for first-time attendees and long-term devotees alike. Rich reviews the many festival highlights, from Jane Campion’s reinvention of the Western in The Power of the Dog to Todd Haynes’ archival documentary The Velvet Underground. Childhood takes center stage in new films from Céline Sciamma and Kenneth Branagh while misunderstood masculinity emerges as a theme in Michael Pearce’s Encounter, Asghar Farhadi’s A Hero, and Mike Mills’s C’mon C’mon. Including a coda on the New York Film Festival, Rich concludes that the masterful riches of the two festivals augur well for the fall 2021 season.

scholarly journals Biogenic Amines in Plant-Origin Foods: Are They Frequently Underestimated in Low-Histamine Diets?

Foods ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 205 ◽  
Author(s):  
Sònia Sánchez-Pérez ◽  
Oriol Comas-Basté ◽  
Judit Rabell-González ◽  
M. Veciana-Nogués ◽  
M. Latorre-Moratalla ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Biogenic Amine ◽  
Plasma Levels ◽  
Diamine Oxidase ◽  
Great Variability ◽  
Plant Origin ◽  
Lower Affinity ◽  
Origin And Evolution ◽  
Histamine Intolerance ◽  
Existing Data

Low-histamine diets are currently used to reduce symptoms of histamine intolerance, a disorder in histamine homeostasis that increases plasma levels, mainly due to reduced diamine-oxidase (DAO) activity. These diets exclude foods, many of them of plant origin, which patients associate with the onset of the symptomatology. This study aimed to review the existing data on histamine and other biogenic amine contents in nonfermented plant-origin foods, as well as on their origin and evolution during the storage or culinary process. The only plant-origin products with significant levels of histamine were eggplant, spinach, tomato, and avocado, each showing a great variability in content. Putrescine has been found in practically all plant-origin foods, probably due to its physiological origin. The high contents of putrescine in certain products could also be related to the triggering of the symptomatology by enzymatic competition with histamine. Additionally, high spermidine contents found in some foods should also be taken into account in these diets, because it can also be metabolized by DAO, albeit with a lower affinity. It is recommended to consume plant-origin foods that are boiled or are of maximum freshness to reduce biogenic amine intake.

The Qualitative and Quantitative Distribution of Plankton in the Strait of Georgia in Relation to Certain Oceanographic Factors

1957 ◽  
Vol 14 (4) ◽  
pp. 521-552 ◽  
Author(s):  
Joseph Eugène Henri Légaré
Keyword(s):  
Salinity Gradient ◽  
General Distribution ◽  
Fraser River ◽  
Strait Of Georgia ◽  
Quantitative Distribution ◽  
Qualitative And Quantitative ◽  
Chemical Factors ◽  
Physical And Chemical ◽  
Plankton Communities ◽  
Made In

In order to gain some picture of the seasonal variations in the plankton communities two cruises were made in the Strait of Georgia, one in June 1955, and the other in November 1955; 165 plankton collections were taken, also surface temperatures.The correlation of these data have resulted in a number of conclusions concerning the distribution of plankton in the Strait of Georgia. The chief factor affecting the general distribution of plankton is the salinity gradient. The inflow of fresh water from the Fraser River forms zones of varying properties, and leads to the development of different plankton communities. The extent to which physical and chemical factors may determine the presence or absence of certain organisms from the zones described is discussed.

scholarly journals ISMB 2003 Bio-ontologies SIG and Sixth Annual Bio-ontologies Meeting Report

2003 ◽  
Vol 4 (6) ◽  
pp. 663-666 ◽  
Author(s):  
Phillip Lord ◽  
Robert Stevens
Keyword(s):  
North America ◽  
Peer Review ◽  
Interest Group ◽  
Text Analysis ◽  
Special Interest ◽  
Text Processing ◽  
Global Politics ◽  
Meeting Report ◽  
The Many ◽  
First Time

The Annual Bio-Ontologies meeting (http://www.cs.man.ac.uk/˜stevens/meeting03/) has now been running for 6 consecutive years, as a special interest group (SIG) of the much larger ISMB conference. It met in Brisbane, Australia, this summer, the first time it was held outside North America or Europe. The bio-ontologies meeting is 1 day long and normally has around 100 attendees. This year there were many fewer, no doubt a result of the distance, global politics and SARS. The meeting consisted of a series of 30 min talks with no formal peer review or publication. Talks ranged in style from fairly formal and complete pieces of work, through works in progress, to the very informal and discursive. Each year's meeting has a theme and this year it was ‘ontologies, and text processing’. There is a tendency for those submitting talks to ignore the theme completely, but this year's theme obviously struck a chord, as half the programme was about ontologies and text analysis (http://www.cs.man.ac.uk/˜stevensr/meeting03/programme.html). Despite the smaller size of the meeting, the programme was particularly strong this year, meaning that the tension between allowing time for the many excellent talks, discussion and questions from the floor was particular keenly felt. A happy problem to have!

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals Work: An Occupational ABC by K. Hatanaka

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Debbie Feisst
Keyword(s):  
Young Adult ◽  
Young Children ◽  
Short Description ◽  
Young Adult Fiction ◽  
University Of Alberta ◽  
Full Page ◽  
Coffee Table ◽  
The Many ◽  
The University ◽  
First Time

Hatanaka, Kellen. Work: An Occupational ABC. Toronto: Groundwood Books, 2014. Print.At first glance, you may look at this book and think it just like the myriad of other ABC books for young children and preschoolers, but you would be delightfully wrong!  Yes, of course, each letter from A to Z is represented and indeed each letter is accompanied by an image that characterizes the letter.  But that is where the similarities end. Toronto-based designer, illustrator and artist Kellen Hatanaka, who can now also call himself a first time author, has created a beautiful and unexpected piece of art for the youngest readers and it is as much a delight to read as it is to behold.  With creamy paper and softly-coloured images, created digitally after first creating ‘hand-drawn patterns and images’, it is reminiscent of a small person’s version of a coffee table book, if said coffee table were in a nursery.It starts out simple enough, with A for Aviator and a full page spread with a boldly-coloured A as well as mountain tops that echo the A’s shape.  The next page, however, alerts the reader to the fun in store – B is for Butcher, shown chasing after a group of raccoons who have absconded with a string of sausages. Yes, there are occupations like Grocer and Tailor but there are also the Ice Cream Vendor, K-9 Officer and the Wedding Singer! What’s wonderful to note are the many alternative occupations, some that you may have not have heard of, and that there is a nice mix of women, men, and cultures taking part. For the curious reader, there is a section of funny ‘Want Ads’ which provides a short description of the occupation.This would make a wonderful new baby gift and is an excellent addition to a home or public library.Highly recommended: 4 stars out of 4 Reviewer: Debbie FeisstDebbie is a Public Services Librarian at the H.T. Coutts Education Library at the University of Alberta.  When not renovating, she enjoys travel, fitness and young adult fiction.

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