ROS Homeostasis and Plant Salt Tolerance: Plant Nanobiotechnology Updates

Salinity is an issue impairing crop production across the globe. Under salinity stress, besides the osmotic stress and Na+ toxicity, ROS (reactive oxygen species) overaccumulation is a secondary stress which further impairs plant performance. Chloroplasts, mitochondria, the apoplast, and peroxisomes are the main ROS generation sites in salt-stressed plants. In this review, we summarize ROS generation, enzymatic and non-enzymatic antioxidant systems in salt-stressed plants, and the potential for plant biotechnology to maintain ROS homeostasis. Overall, this review summarizes the current understanding of ROS homeostasis of salt-stressed plants and highlights potential applications of plant nanobiotechnology to enhance plant tolerance to stresses.

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Improvement of plant performance under water deficit with the employment of biological and chemical priming agents

The Journal of Agricultural Science ◽

10.1017/s0021859618000126 ◽

2018 ◽

Vol 156 (5) ◽

pp. 680-688 ◽

Cited By ~ 9

Author(s):

R. Balestrini ◽

W. Chitarra ◽

C. Antoniou ◽

M. Ruocco ◽

V. Fotopoulos

Keyword(s):

Mycorrhizal Fungi ◽

Crop Production ◽

Current Knowledge ◽

Arbuscular Mycorrhizal ◽

Plant Performance ◽

Plant Tolerance ◽

Climate Change Research ◽

Chemical Agents ◽

Chemical Priming

AbstractDrought represents one of the major constraints on agricultural productivity and food security and in future is destined to spread widely as a consequence of climate change. Research efforts are focused on developing strategies to make crops more resilient and to mitigate the effects of stress on crop production. In this context, the use of root-associated microbial communities and chemical priming strategies able to improve plant tolerance to abiotic stresses, including drought, have attracted increasing attention in recent years. The current review offers an overview of recent research aimed at verifying the role of arbuscular mycorrhizal fungi and chemical agents to improve plant tolerance to drought and to highlight the mechanisms involved in this improvement. Attention will be devoted mainly to current knowledge on the mechanisms involved in water transport.

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Nanoceria seed priming enhanced salt tolerance in rapeseed through modulating ROS homeostasis and α-amylase activities

Journal of Nanobiotechnology ◽

10.1186/s12951-021-01026-9 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Mohammad Nauman Khan ◽

Yanhui Li ◽

Zaid Khan ◽

Linlin Chen ◽

Jiahao Liu ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Crop Production ◽

Germination Rate ◽

Plant Stress ◽

Seed Priming ◽

Ros Homeostasis ◽

Plant Stress Tolerance ◽

Underlying Mechanisms ◽

Plant Salt Tolerance

Abstract Background Salinity is a big threat to agriculture by limiting crop production. Nanopriming (seed priming with nanomaterials) is an emerged approach to improve plant stress tolerance; however, our knowledge about the underlying mechanisms is limited. Results Herein, we used cerium oxide nanoparticles (nanoceria) to prime rapeseeds and investigated the possible mechanisms behind nanoceria improved rapeseed salt tolerance. We synthesized and characterized polyacrylic acid coated nanoceria (PNC, 8.5 ± 0.2 nm, −43.3 ± 6.3 mV) and monitored its distribution in different tissues of the seed during the imbibition period (1, 3, 8 h priming). Our results showed that compared with the no nanoparticle control, PNC nanopriming improved germination rate (12%) and biomass (41%) in rapeseeds (Brassica napus) under salt stress (200 mM NaCl). During the priming hours, PNC were located mostly in the seed coat, nevertheless the intensity of PNC in cotyledon and radicle was increased alongside with the increase of priming hours. During the priming hours, the amount of the absorbed water (52%, 14%, 12% increase at 1, 3, 8 h priming, respectively) and the activities of α-amylase were significantly higher (175%, 309%, 295% increase at 1, 3, 8 h priming, respectively) in PNC treatment than the control. PNC primed rapeseeds showed significantly lower content of MDA, H2O2, and •O2− in both shoot and root than the control under salt stress. Also, under salt stress, PNC nanopriming enabled significantly higher K+ retention (29%) and significantly lower Na+ accumulation (18.5%) and Na+/K+ ratio (37%) than the control. Conclusions Our results suggested that besides the more absorbed water and higher α-amylase activities, PNC nanopriming improves salt tolerance in rapeseeds through alleviating oxidative damage and maintaining Na+/K+ ratio. It adds more knowledge regarding the mechanisms underlying nanopriming improved plant salt tolerance. Graphical abstract

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Hypoxic Radioresistance: Can ROS Be the Key to Overcome It?

Cancers ◽

10.3390/cancers11010112 ◽

2019 ◽

Vol 11 (1) ◽

pp. 112 ◽

Cited By ~ 19

Author(s):

Hui Wang ◽

Heng Jiang ◽

Melissa Van De Gucht ◽

Mark De Ridder

Keyword(s):

Reactive Oxygen Species ◽

Cancer Cells ◽

Tumor Cells ◽

Reactive Oxygen ◽

Signaling Molecules ◽

Antioxidant Systems ◽

Oxygen Species ◽

Ros Homeostasis ◽

Hypoxic Tumor ◽

Radiotherapy Failure

Radiotherapy is a mainstay treatment for many types of cancer and kills cancer cells via generation of reactive oxygen species (ROS). Incorporating radiation with pharmacological ROS inducers, therefore, has been widely investigated as an approach to enhance aerobic radiosensitization. However, this strategy was overlooked in hypoxic counterpart, one of the most important causes of radiotherapy failure, due to the notion that hypoxic cells are immune to ROS insults because of the shortage of ROS substrate oxygen. Paradoxically, evidence reveals that ROS are produced more in hypoxic than normoxic cells and serve as signaling molecules that render cells adaptive to hypoxia. As a result, hypoxic tumor cells heavily rely on antioxidant systems to sustain the ROS homeostasis. Thereby, they become sensitive to insults that impair the ROS detoxification network, which has been verified in diverse models with or without radiation. Of note, hypoxic radioresistance has been overviewed in different contexts. To the best of our knowledge, this review is the first to systemically summarize the interplay among radiation, hypoxia, and ROS, and to discuss whether perturbation of ROS homeostasis could provide a new avenue to tackle hypoxic radioresistance.

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Nanoceria Seed Priming Improves Salt Tolerance in Rapeseed Through Modulating ROS Homeostasis and α-Amylase Activities

10.21203/rs.3.rs-642905/v1 ◽

2021 ◽

Author(s):

Mohammad Nauman Khan ◽

Yanhui Li ◽

Zaid Khan ◽

Linlin Chen ◽

Jiahao Liu ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Crop Production ◽

Germination Rate ◽

Plant Stress ◽

Seed Priming ◽

Ros Homeostasis ◽

Plant Stress Tolerance ◽

Underlying Mechanisms ◽

Plant Salt Tolerance

Abstract Background: Salinity is a big threat to agriculture by limiting crop production. Nanopriming (seed priming with nanomaterials) is an emerged approach to improve plant stress tolerance; however, our knowledge about the underlying mechanisms is limited. Results: We used cerium oxide nanoparticles (nanoceria) to prime rapeseeds and investigated the possible mechanisms behind nanoceria improved rapeseed salt tolerance. We synthesized and characterized polyacrylic acid coated nanoceria (PNC, 8.5 ± 0.2 nm, -43.3 ± 6.3 mV) and monitored its distribution in different tissues of the seed during the imbibition period (1, 3, 8h priming). Our results showed that compared with the no nanoparticle control, PNC nanopriming improved germination rate (12%) and biomass (41%) in rapeseeds under salt stress (200 mM NaCl). During the priming hours, PNC were located mostly in the seed coat, nevertheless the intensity of PNC in cotyledon and radicle was increased alongside with the increase of priming hours. During the priming hours, the amount of the absorbed water (52%, 14%, 12% increase at 1, 3, 8h priming, respectively) and the activities of α-amylase were significantly higher (175%, 309%, 295% increase at 1, 3, 8h priming, respectively) in PNC treatment than the control. PNC primed rapeseeds showed significantly lower content of MDA, H2O2, and •O2— in both shoot and root than the control under salt stress. Also, under salt stress, PNC nanopriming enabled significantly higher K+ retention (29%) and also significantly lower Na+ accumulation (18.5%) and Na+/K+ ratio (37%) than the control. Conclusions: Our results suggested that besides the more absorbed water and increased α-amylase activities, PNC nanopriming improves salt tolerance in rapeseeds through maintaining ROS homeostasis and Na+/K+ ratio. It adds more knowledge regarding the mechanisms underlying nanopriming improved plant salt tolerance.

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Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

Journal of Health and Allied Sciences NU ◽

10.1055/s-0040-1715995 ◽

2020 ◽

Vol 10 (03) ◽

pp. 109-115

Author(s):

Shreya Udaya ◽

Nishith Babu ◽

Dechamma Pandyanda Nanjappa ◽

Krithika Kalladka ◽

Gunimala Chakraborty ◽

...

Keyword(s):

Aqueous Extract ◽

Antioxidant Properties ◽

Ethanol Extract ◽

Antioxidant Property ◽

Stem Bark ◽

Oxygen Species ◽

Cassia Fistula ◽

Potential Applications ◽

Ethanol Extracts ◽

Stress Models

Abstract Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish. Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark. Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

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Reactive Oxygen Species in Host Plant Are Required for an Early Defense Response against Attack of Stagonospora nodorum Berk. Necrotrophic Effectors SnTox

Plants ◽

10.3390/plants10081586 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1586

Author(s):

Svetlana Veselova ◽

Tatyana Nuzhnaya ◽

Guzel Burkhanova ◽

Sergey Rumyantsev ◽

Igor Maksimov

Keyword(s):

Reactive Oxygen Species ◽

Early Stage ◽

Reactive Oxygen ◽

Triticum Aestivum L ◽

Redox Status ◽

Stagonospora Nodorum ◽

Ros Generation ◽

Ros Production ◽

Oxygen Species ◽

Necrotrophic Effectors

Reactive oxygen species (ROS) play a central role in plant immune responses. The most important virulence factors of the Stagonospora nodorum Berk. are multiple fungal necrotrophic effectors (NEs) (SnTox) that affect the redox-status and cause necrosis and/or chlorosis in wheat lines possessing dominant susceptibility genes (Snn). However, the effect of NEs on ROS generation at the early stages of infection has not been studied. We studied the early stage of infection of various wheat genotypes with S nodorum isolates -Sn4VD, SnB, and Sn9MN, carrying a different set of NE genes. Our results indicate that all three NEs of SnToxA, SnTox1, SnTox3 significantly contributed to cause disease, and the virulence of the isolates depended on their differential expression in plants (Triticum aestivum L.). The Tsn1–SnToxA, Snn1–SnTox1and Snn3–SnTox3 interactions played an important role in inhibition ROS production at the initial stage of infection. The Snn3–SnTox3 inhibited ROS production in wheat by affecting NADPH-oxidases, peroxidases, superoxide dismutase and catalase. The Tsn1–SnToxA inhibited ROS production in wheat by affecting peroxidases and catalase. The Snn1–SnTox1 inhibited the production of ROS in wheat by mainly affecting a peroxidase. Collectively, these results show that the inverse gene-for gene interactions between effector of pathogen and product of host sensitivity gene suppress the host’s own PAMP-triggered immunity pathway, resulting in NE-triggered susceptibility (NETS). These results are fundamentally changing our understanding of the development of this economical important wheat disease.

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CBIO-07. CELL DEATH INDUCED BY AN OSCILLATING MAGNETIC FIELD IN PATIENT DERIVED GLIOBLASTOMA CELLS IS MEDIATED BY REACTIVE OXYGEN SPECIES

Neuro-Oncology ◽

10.1093/neuonc/noaa215.067 ◽

2020 ◽

Vol 22 (Supplement_2) ◽

pp. ii17-ii17

Author(s):

Shashank Hambarde ◽

Martyn Sharpe ◽

David Baskin ◽

Santosh Helekar

Keyword(s):

Reactive Oxygen Species ◽

Cell Death ◽

Cell Viability ◽

Cortical Neurons ◽

Reactive Oxygen ◽

Great Promise ◽

Antioxidant Vitamin ◽

Ros Generation ◽

Oxygen Species ◽

Novel Therapy

Abstract Noninvasive cancer therapy with minimal side effects would be ideal for improving patient outcome in the clinic. We have developed a novel therapy using strong rotating magnets mounted on a helmet. They generate oscillating magnetic fields (OMF) that penetrate through the skull and cover the entire brain. We have demonstrated that OMF can effectively kill patient derived glioblastoma (GBM) cells in cell culture without having cytotoxic effects on cortical neurons and normal human astrocytes (NHA). Exposure of GBM cells to OMF reduced the cell viability by 33% in comparison to sham-treated cells (p< 0.001), while not affecting NHA cell viability. Time lapse video-microscopy for 16 h after OMF exposure showed a marked elevation of mitochondrial reactive oxygen species (ROS), and rapid apoptosis of GBM cells due to activation of caspase 3. Addition of a potent antioxidant vitamin E analog Trolox effectively blocked OMF-induced GBM cell death. Furthermore, OMF significantly potentiated the cytotoxic effect of the pro-oxidant Benzylamine. The results of our studies demonstrate that OMF-induced cell death is mediated by ROS generation. These results demonstrate a potent oncolytic effect on GBM cells that is novel and unrelated to any previously described therapy, including a very different mechanism of action and different technology compared to Optune therapy. The effect is very powerful, and unlike Optune, can be seen within hours after initiation of treatment. We believe that this technology holds great promise for new, effective and nontoxic treatment of glioblastoma.

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Potential applications of biogenic selenium nanoparticles in alleviating biotic and abiotic stresses in plants: A comprehensive insight on the mechanistic approach and future perspectives

Green Processing and Synthesis ◽

10.1515/gps-2021-0047 ◽

2021 ◽

Vol 10 (1) ◽

pp. 456-475

Author(s):

Efat Zohra ◽

Muhammad Ikram ◽

Ahmad A. Omar ◽

Mujahid Hussain ◽

Seema Hassan Satti ◽

...

Keyword(s):

Abiotic Stresses ◽

Crop Production ◽

Plant Secondary Metabolites ◽

Selenium Nanoparticles ◽

Future Research ◽

Biotic And Abiotic Stresses ◽

Biotic Stresses ◽

Agriculture Sector ◽

Developmental Growth ◽

Potential Applications

Abstract In the present era, due to the increasing incidence of environmental stresses worldwide, the developmental growth and production of agriculture crops may be restrained. Selenium nanoparticles (SeNPs) have precedence over other nanoparticles because of the significant role of selenium in activating the defense system of plants. In addition to beneficial microorganisms, the use of biogenic SeNPs is known as an environmentally friendly and ecologically biocompatible approach to enhance crop production by alleviating biotic and abiotic stresses. This review provides the latest development in the green synthesis of SeNPs by using the results of plant secondary metabolites in the biogenesis of nanoparticles of different shapes and sizes with unique morphologies. Unfortunately, green synthesized SeNPs failed to achieve significant attention in the agriculture sector. However, research studies were performed to explore the application potential of plant-based SeNPs in alleviating drought, salinity, heavy metal, heat stresses, and bacterial and fungal diseases in plants. This review also explains the mechanistic actions that the biogenic SeNPs acquire to alleviate biotic and abiotic stresses in plants. In this review article, the future research that needs to use plant-mediated SeNPs under the conditions of abiotic and biotic stresses are also highlighted.

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Multifunctional Role of Chitosan Edible Coatings on Antioxidant Systems in Fruit Crops: A Review

International Journal of Molecular Sciences ◽

10.3390/ijms22052633 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2633

Author(s):

Giuseppina Adiletta ◽

Marisa Di Matteo ◽

Milena Petriccione

Keyword(s):

State Of The Art ◽

Antioxidant Systems ◽

Edible Coatings ◽

Ros Production ◽

Oxygen Species ◽

Fruit Crops ◽

Oxidative Damages ◽

Film Forming ◽

Anti Oxidant

Chitosan-based edible coatings represent an eco-friendly and biologically safe preservative tool to reduce qualitative decay of fresh and ready-to-eat fruits during post-harvest life due to their lack of toxicity, biodegradability, film-forming properties, and antimicrobial actions. Chitosan-based coatings modulate or control oxidative stress maintaining in different manner the appropriate balance of reactive oxygen species (ROS) in fruit cells, by the interplay of pathways and enzymes involved in ROS production and the scavenging mechanisms which essentially constitute the basic ROS cycle. This review is carried out with the aim to provide comprehensive and updated over-view of the state of the art related to the effects of chitosan-based edible coatings on anti-oxidant systems, enzymatic and non-enzymatic, evaluating the induced oxidative damages during storage in whole and ready-to-eat fruits. All these aspects are broadly reviewed in this review, with particular emphasis on the literature published during the last five years.

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Metabolic Adjustment of Glycine max (L.) Merril in the Presence of Nitrate and Bradyrhizobium japonicum

Agronomy ◽

10.3390/agronomy11081518 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1518

Author(s):

Alberto Mongolo Júnior ◽

Felipe Girotto Campos ◽

Gustavo Ribeiro Barzotto ◽

Jonas Akenaton Venturineli Pagassini ◽

Maria Aparecida Ribeiro Vieira ◽

...

Keyword(s):

Bradyrhizobium Japonicum ◽

Dry Mass ◽

Antioxidant Systems ◽

Oxygen Species ◽

Membrane Stabilization ◽

Total Sugars ◽

Vegetative Phase ◽

Glycine Max L ◽

Metabolic Adjustment ◽

Plant Dry Mass

Reactive oxygen species are generated during the processes of photosynthesis and nitrate reduction, which can compromise the integrity of biomolecules and membranes. During the vegetative phase of Fabaceae species, around half of translocated carbohydrate is used for nodule growth, while the other half returns to the aerial part with nitrogen incorporated. These sugars may be yet involved with membrane stabilization, signaling, and activation of important genetic pathways for plant development. Thus, the aim was to study the adjustments of the photosynthetic and antioxidant systems and the accumulation of carbohydrates and biomass in Glycine–Bradyrhizobium cultivated with nitrate (NO3−). Four treatments were evaluated in completely randomized blocks. Glycine–Bradyrhizobium was grown with 1.7 mM of NO3− (GB: 1.7 mM NO3−) and without NO3− (GB: 0 mM NO3−), and Glycine was grown with 1.7 mM of NO3− (G: 1.7 mM NO3−) and without NO3− (G: 0 mM NO3−). Glycine–Bradyrhizobium symbiosis contributes to photosynthetic metabolism and total sugars, reduces the action of antioxidant enzymes, and minimizes the use of nitrate in soybean cultivation.; Glycine–Bradyrhizobium with nitrate provided greater plant dry mass in the vegetative phase, along with increased enzymatic activity and reduced nodule mass.

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