scholarly journals Evaluation of the Swallow-Tail Sign and Correlations of Neuromelanin Signal with Susceptibility and Relaxations

Tomography ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 107-119
Author(s):  
Tzu-Wei Lee ◽  
Cheng-Yu Chen ◽  
Kuan Chen ◽  
Chao-Wei Tso ◽  
Hui-Hsien Lin ◽  
...  
Keyword(s):  
Magnetization Transfer ◽  
Systematic Evaluation ◽  
Joint Analysis ◽  
Quantitative Susceptibility Mapping ◽  
Widespread Application ◽  
Hyperintense Signal ◽  
Significant Difference ◽  
Relaxation Measurements ◽  
Iron Deposits ◽  
Older Subjects

The presence of a swallow-tail sign in the nigrosome-1 with hyperintense signal shown on the susceptibility-weighted imaging (SWI) has been shown to be sensitive in detecting the abnormal iron deposits in this area. A systematic evaluation in healthy subjects is required before this tool can be recommended in a widespread application. We evaluated a simple and practical SWI approach using a multiecho gradient-echo sequence with an improved contrast-to-noise ratio (CNR). We also evaluated the association of the neuromelanin imaging contrast behavior with the susceptibility and relaxation measurements. Twenty-five older and 23 young healthy adults were evaluated. The CNRs of the nigrosome-1 were compared along with method and group. Correlations of the nigrosome-1 neuromelanin signal in the neuromelanin-sensitive imaging with CNRs in the susceptibility, T1 and T2 mappings were examined. Two different coils were used to confirm the reproducibility. Compared with the single-echo, multiecho SWI can improve the CNR of the swallow-tail sign. We found significant correlations between neuromelanin signal and CNRs in the susceptibility and T2 mappings, and T1 value. The older subjects exhibited increased CNRs compared with the young adults. No significant difference was observed in the measurements between 20 and 64 channels. The multiecho technique allows the high-quality nigrosome-1 images in SWI and allows for a joint analysis of T2* and quantitative-susceptibility mapping at high spatial resolution. The correlations of neuromelanin-sensitive imaging with susceptibility and T2 imply that the iron content in the nigrosome-1 may have significant influences on the hyperintensity of neuromelanin in the magnetization transfer-related contrast.

Fatal "Iron Heart" in an Adolescent: Biochemical and Ultrastructural Aspects of the Heart

PEDIATRICS ◽  
1975 ◽  
Vol 55 (3) ◽  
pp. 336-341
Author(s):  
Shyamal K. Sanyal ◽  
Warren Johnson ◽  
B. Jayalakshmamma ◽  
Alexander A. Green
Keyword(s):  
Myocardial Dysfunction ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Right Atrial ◽  
Left Ventricular Myocardium ◽  
Biochemical Basis ◽  
Iron Storage ◽  
Ultrastructural Studies ◽  
Significant Difference ◽  
Iron Deposits

Histochemical and ultrastructural aspects of the heart were investigated in an adolescent with fatal congestive heart failure resulting from exogenous hemochromatosis. Extensive iron deposits were found in all four chambers, papillary muscles, and the conduction system. These deposits were most prominent over the outer third of the left ventricular myocardium, with no significant difference between deposits in the middle and inner thirds. Quantitative analysis of iron from different chambers and all zones of the left ventricular myocardium confirmed the aforementioned pattern of iron distribution. Iron deposits in sinoauricular and atrioventricular nodes were similar to those in the right atrial myocardium. Degenerative changes and fibrosis were minimal. Ultrastructural studies showed that intracytoplasmic iron deposition followed a perinuclear, paranuclear, or diffuse pattern. In addition, some iron was consistently present in the nucleus and mitochondria. It is postulated that the presence of iron in the mitochondria may adversely affect the cellular enzyme system; this could provide a biochemical basis for myocardial dysfunction in patients with acquired iron-storage disease.

scholarly journals Results of the infrasonic array on the Valaam Island primary data processing

2020 ◽  
Vol 2 (1) ◽  
pp. 85-91
Author(s):  
Vladimir Karpinsky ◽  
Vladimir Asming
Keyword(s):  
Data Processing ◽  
Seismic Station ◽  
Sampling Rate ◽  
Low Frequency ◽  
Primary Data ◽  
Joint Analysis ◽  
Main Task ◽  
Significant Difference ◽  
Infrasound Signal ◽  
Short Time

The infrasound array VALS developed in Kola Branch GS RAS has been installed in June 2016 on the Valaam Island in addition to the continuously operating seismic station VALR. The array consists of 3 spaced low-frequency microphones. The data with a sampling rate of 100 Hz is stored continuously at the acquisition computer; the timing is carried out using GPS. In addition to the acquisition system, an infrasound signal detector is installed on the computer. It works in near real-time mode and enables us to find signals and compute their back azimuths. At the end of 2018, a new version of the detector was developed at the Kola Branch GS RAS. The detector began to work much faster, which enabled us to carry out data processing for 2.5 years in two frequency ranges in a short time. The main task of the array is acoustic monitoring, the detection of infrasound events, the determination of their parameters, and the selection of events of natural origin. The data are also used (in combination with the VALR seismic station data) to locate near seismic events, especially weak ones. The analysis of the obtained data revealed the prevailing directions to the signal sources. The change of directions to sources in time was investigated, seasonal features were revealed. Acoustic events were detected in the frequency bands 1–5 Hz and 10–20 Hz, and a significant difference was found in the azimuthal distribution of events for these ranges. A joint analysis of acoustic and seismic data showed that the part of events with both acoustic and seismic components is low – it is almost completely exhausted by career explosions. It was also noted that in addition to explosions in nearby quarries (Kuznechnoye, Pitkäranta) located at a distance of 50–60 km, according to acoustic data, events corresponding to explosions at quarries located at a distance of 100 km or more were repeatedly identified.

Wais Verbal-Performance Differences among Elderly When Controlling for Fatigue

1979 ◽  
Vol 44 (2) ◽  
pp. 489-490 ◽  
Author(s):  
James O. Rust ◽  
Danna Barnard ◽  
Gerald D. Oster
Keyword(s):  
Performance Differences ◽  
Significant Difference ◽  
Scale Scores ◽  
Older Subjects ◽  
Order Of Presentation ◽  
Performance Scale ◽  
Verbal Performance

Older subjects show consistent declines on Performance Scale scores of the WAIS. Data were collected from 22 men and 22 women over 65 yr. to determine whether the lower scores were due to decreased abilities or to task-related fatigue. Fatigue was controlled by counterbalancing the order of presentation of the WAIS scales. The Performance Scale was administered first on one-half of the protocols. Although the Verbal IQs were significantly higher than the Performance IQs, no significant difference was noted for order of presentation.

Phamacokinetics (PK) Substudy of Rituximab in a Prospective Clinical Trial for Pediatric Chronic Immune Thrombocytopenic Purpura (cITP).

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1243-1243 ◽  
Author(s):  
Carolyn M. Bennett ◽  
James B. Bussel ◽  
Brigitta U. Mueller ◽  
Thomas C. Abshire ◽  
Hadi Sawaf ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Pediatric Patients ◽  
Compartment Model ◽  
Volume Of Distribution ◽  
Half Time ◽  
Age Cohorts ◽  
Elimination Half ◽  
Significant Difference ◽  
Older Subjects ◽  
The Difference

Abstract Rationale: Rituximab dosing in pediatric patients is derived from body surface area-based “standard” adult lymphoma regimens. PK data for rituximab in children are lacking. Methods: We evaluated serum rituximab levels in a subset of 36 children and adolescents with severe cITP treated on a prospective phase I/II study. Patients received four weekly infusions, 375mg/m2/dose. Trough and 30-minute, 24- and 48-hour (hr) post-infusion levels were obtained with doses 1 and 4, and 7 days after dose 1. PK parameters were obtained separately for each patient at the 1-wk and 4-wk infusions by fitting a two-compartment model. Two-factor analysis of variance was employed to compare parameters between younger (2–9 years) and older (10–18 years) subjects and between responders and non-responders at the two infusion times. Results: Fourteen patients had PK measurements at week 1 and eleven at week 4 (Table). The median initial volume of distribution (V1), for all patients, calculated by back-extrapolation to t0, approximated plasma volume, 53 mL/kg. Kinetics fit a two-component model, with relatively rapid initial half-time (exit) reflecting distribution out of plasma space, and equilibration representing slow return into the vascular pool. The drug elimination half-time was too slow to observe in these 7-day periods. Apparent half-time for initial redistribution from the plasma space was significantly longer for younger subjects at week 4 than at week 1 and than for older subjects at week 4 (Table). By comparison, in studies of 14 adults with lymphoma treated with rituximab at the same dosage regimen, the manufacturer reports mean serum half-time of 76 hrs (range, 31–153) at week 1 and 205 hrs (range, 84 – 407) at week 4. In this PK sub-study, we found no significant difference in kinetic parameters between the 5 responders (defined by sustained platelet count >50,000/mm3 at week 12) and 11 non-responders. Conclusion: In this small number of pediatric patients with cITP treated with 4 weekly infusions of rituximab, the half-time was longer for younger subjects at week 4 than week 1 and than for older subjects at week 4. Trough levels at week 4 do not represent steady state because the elimination half-time is long. The reason for the difference between the two age cohorts is not readily apparent, but we propose that the shorter exit half-time in younger patients at week 1 compared to week 4 corresponds to a higher number of initially accessible CD20 binding sites (B cells) in young children. PK parameters of rituximab distribution at weeks 1 and 4 Age (yr) Week 1 n Week4 n Median (min, max) Median (min, max) *Age x week interaction significant in two-way analysis of variance, p<0.015 V1 (mL/kg) All 53 (35–76) 14 54 (35–74) 11 2–9 54 (43–76) 7 62 (54–74) 4 10–18 52 (35–73) 7 49 (35–62) 7 Exit Half-time (hr) All 49 (16–83) 14 83 (25–193) 11 2–9 45 (31–55) 7 100 (83–193)* 4 10–18 57 (16–83) 7 57 (25–97) 7

Investigating Optimal Echo Times for Quantitative Susceptibility Mapping of Basal Ganglia Nuclei in the Healthy Brain

2020 ◽  
Vol 16 (8) ◽  
pp. 991-996
Author(s):  
Wenping Fan ◽  
Xue Wang ◽  
Xingwen Zhang ◽  
Mengqi Liu ◽  
Qinglin Meng ◽  
...  
Keyword(s):  
Magnetic Susceptibility ◽  
Clinical Practice ◽  
Standard Deviation ◽  
Basal Ganglia ◽  
Caudate Nucleus ◽  
Susceptibility Mapping ◽  
Quantitative Susceptibility Mapping ◽  
Significant Difference ◽  
The Mean ◽  
Echo Time

Background: Quantitative susceptibility mapping (QSM) technique had been used to measure the magnetic susceptibility of brain tissue in clinical practice. However, QSM presented echo-time (TE) dependence, and an appropriate number of echo-times (nTEs) for QSM became more important to obtain the reliable susceptibility value. Objective: The aim of the study was to explore the optimal nTEs for quantitative susceptibility mapping (QSM) measurements of basal ganglia nuclei in the healthy brain. Methods: 3D multi-echo enhanced gradient recalled echo T2 star weighted angiography (ESWAN) sequence was acquired on a 3.0T MR scanner for QSM analysis. Regions of interests (ROIs) were drawn along the margin of the head of the caudate nucleus (HCN), putamen (Pu) and globus pallidus (GP). The mean susceptibility value and standard deviation of the ROIs were derived from the pixels within each region. Results: CV analysis demonstrated that TE6, TE8 and TE14 ESWAN sequences presented consistent lower CV value (<1) for QSM measure of HCN, Pu and GP. ANOVA identified that susceptibility value showed no significant difference between TE6 and TE8 in HCN, Pu and GP (P > 0.05). ICC analysis demonstrated that the susceptibility value of TE6-TE8 had the highest ICC value as compared with TE6-TE14 and TE8-TE14 in HCN, Pu and GP. Combined with the timeefficiency of MRI scanning, TE6 sequence could not only provide the reliable QSM measurement but also short imaging time. Conclusions: The current study identified that the optimal nTEs of ESWAN were 6 TEs (2.9ms ~ 80.9ms) for QSM measurement of basal ganglia nuclei in the healthy brain.

scholarly journals AB0938 PARTICULARITIES OF INFECTIOUS SPONDYLODISCITIS IN THE ELDERLY: COMPARATIVE STUDY WITH THE YOUNG SUBJECT

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1768.2-1768
Author(s):  
L. Ben Ammar ◽  
A. Ben Tekaya ◽  
M. Ben Hammamia ◽  
O. Saidane ◽  
S. Bouden ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Compression ◽  
Cauda Equina ◽  
The Elderly ◽  
Cord Compression ◽  
Elderly Subjects ◽  
Significant Difference ◽  
Infectious Spondylodiscitis ◽  
Older Subjects ◽  
Young Subjects

Background:The incidence of infectious spondylodiscitis is steadily increasing. The prognosis depends on various parameters, including age.Objectives:To explore the differences in presentation and the results of further investigations and the prognosis of spondylodiscitis between young and elderly subjects.Methods:This is a retrospective study of 113 patients admitted to our department over a period of 20 years [1998-2018]. The epidemiological, clinical, biological, radiological and progressive aspects of spondylodiscitis in subjects over the age of 65 were compared to cases of spondylodiscitis in young subjects whose age is less than 65 years.Results:Of the 113 identified patients, 33 (29.2%) were classified as older, with male predominance in both groups (56.2 and 51.5% respectively; p=0.6). The presence of risk factors, particularly diabetes, was more frequent in the older subjects but without a statistically significant difference (p=0.2). Hepathopathies were also more frequent in the elderly but without statistically significant difference (p=0.3). The mean duration of progression was 3 months with no statistically significant difference between the two groups (4 and 5 months respectively, p=0.1).The presence of neurological symptoms, especially radiculalgia, was more frequently reported in younger subjects (p=0.01). Also, young subjects were more likely to have a fever (p=0.005). On the other hand, a biological inflammatory syndrome was more frequently encountered in older subjects (p=0.03).We found no statistically significant difference in the location of spondylodiscitis (p=0.4). Also, multifocal involvement was more frequent in older subjects but without a statistically significant difference.Radiologically, para-vertebral abscesses, epiduritis and spinal cord compression were more common in the elderly group (p=0.03; p=0.01 and p=0.01, respectively). While the frequency of intervertebral pinching and erosions was similar in both groups (p=0.1; p=0.8, respectively).Tuberculosis was the most involved germ in more than 50% of cases with no statistically significant difference between the two groups (57.5 and 51.5% respectively; p=0.5).All patients received targeted antibiotic therapy.The occurrence of immediate complications was more frequent in the elderly but without a statistically significant difference (p=0.1). They included neurological complications (spinal cord compression and Cauda equina syndrome) that occurred in 6.3% of cases in younger subjects versus 36.4% of cases in the elderly (p=0.07). Followed by treatment side effects dominated by disturbance of liver function due mainly to antituberculosis drugs occurring in 16.3% of cases in the younger group and 15.2% of cases in older subjects (p=0.8). Finally, there were 2 deaths in each group (p=0.5).Conclusion:Our series has shown that the picture of spondylodiscitis in the elderly is less noisy. However, the neurological damage is more pronounced on imaging. The misleading symptomatology in the elderly explains the delay in diagnosis and treatment, which leads to more frequent complications and excess mortality.Disclosure of Interests:None declared

Quantitative susceptibility mapping in spinocerebellar ataxia type 3/Machado–Joseph disease (SCA3/MJD)

2019 ◽  
Vol 61 (4) ◽  
pp. 520-527
Author(s):  
Fangfang Xie ◽  
Liao Weihua ◽  
Ouyang Lirong ◽  
Xiaoyi Wang ◽  
Wu Xing
Keyword(s):  
Substantia Nigra ◽  
Globus Pallidus ◽  
Spinocerebellar Ataxia ◽  
Susceptibility Mapping ◽  
Iron Deposition ◽  
Spinocerebellar Ataxia Type ◽  
Quantitative Susceptibility Mapping ◽  
Significant Difference ◽  
Machado Joseph Disease ◽  
Normal Controls

Background The deep nuclei, brainstem, and anterior central gyrus are important sites of spinocerebellar ataxia type3/Machado–Joseph Disease (SCA3/MJD) involvement. These locations were the common iron deposition areas. We hypothesized that iron deposition changes occur in SCA3/MJD patients and are associated with disease progression. Purpose Quantitative susceptibility mapping was used to quantitatively analyze changes in iron levels in SCA3/MJD patients. Material and Methods Eighteen symptomatic SCA3/MJD patients and 18 age-matched normal controls (group 1; NC1), and 12 asymptomatic mutation carriers (pre-SCA3/MJD) and 16 age-matched normal controls (group 2; NC2) were examined by enhanced gradient echo T2*-weighted angiography. Data were processed to obtain the quantitative susceptibility mapping values. Independent sample t-tests were conducted to compare the differences in the quantitative susceptibility mapping values. Results In the red nuclei and substantia nigra, the quantitative susceptibility mapping values of the symptomatic SCA3/MJD group were significantly higher than those of NC1 ( P < 0.05). The quantitative susceptibility mapping values of the symptomatic SCA3/MJD group were higher than those of NC1 in the globus pallidus, but it was not statistically significant ( P = 0.056). No significant difference in quantitative susceptibility mapping values was found between the pre-SCA3/MJD and NC2. No significant correlation was found between the International Cooperative Ataxia Rating Scale and the Scale for the Assessment and Rating of Ataxia and the quantitative susceptibility mapping values. Conclusion The results clearly demonstrated the quantitative susceptibility mapping value increase in the globus pallidus, red nuclei, and substantia nigra of the symptomatic SCA3/MJD group, indicating iron overload in these nuclei, suggesting that iron deposition is associated with disease onset.

scholarly journals Systematic Evaluation of Efficacy and Safety of Acupuncture Treatment for Patients with Atrial Fibrillation

2019 ◽  
Vol 7 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Yu Fei ◽  
Rui Fei ◽  
Jing Zhang ◽  
Yaoyao Sun ◽  
Qiong Yu
Keyword(s):  
Atrial Fibrillation ◽  
Clinical Practice ◽  
Sinus Rhythm ◽  
Acupuncture Treatment ◽  
Systematic Evaluation ◽  
Control Group ◽  
Efficacy And Safety ◽  
Physical And Mental Health ◽  
Negative Effects ◽  
Significant Difference

BACKGROUND: Atrial fibrillation (AF) is one of the most common types of arrhythmia diagnosed in clinical practice. Due to its negative effects on people's physical and mental health, it is necessary to prevent and treat AF. Recently, scholars have found that acupuncture can be used to treat AF, but some scholars have questioned its therapeutic efficacy. AIM: Therefore, this study was performed to assess the efficacy and safety of acupuncture treatment for AF patients. METHODS: Previously published research articles were retrieved from six databases, and the data was analysed using RevMan5.3 software with a statistically significant difference defined as P < 0.05. RESULTS: A total of 8 relevant kinds of literature were retrieved containing 633 AF patients (323 in the treatment group and 310 in the control group). Acupuncture treatment increased the total efficacy and the rate of AF cardioversion to sinus rhythm (RR: 1.38; 95% CI: 1.25 to 1.53 vs RR: 1.40;95% CI: 1.16 to 1.69; each P < 0.05), and decreased the time of AF cardioversion to sinus rhythm, the heart rate and incidence of adverse effects (RR: -3.95; 95% CI: -4.98 to -2.91 vs RR: -14.54; 95% CI: -24.09 to -5.00 vs RR: 0.48; 95% CI: 0.21 to 1.11, each P < 0.05). There was difference between retention time more and less than 30 minutes (I2 = 74.9%, P = 0.05). The funnel plot displayed a symmetrical and funnel-form shape, indicating low bias. CONCLUSION: Acupuncture has a good therapeutic effect and safety profile on patients with AF, and its application in clinical practice should be considered.

scholarly journals Comparison of Dose and Effectiveness of a Single-Session Ultrasound-Guided High-Intensity Focused Ultrasound Ablation of Uterine Fibroids With Different Sizes

2021 ◽  
Vol 11 ◽  
Author(s):  
Mei-Jie Yang ◽  
Ren-Qiang Yu ◽  
Jin-Yun Chen ◽  
Zhi-Biao Wang
Keyword(s):  
High Intensity ◽  
Focused Ultrasound ◽  
Uterine Fibroids ◽  
Volume Ratio ◽  
High Intensity Focused Ultrasound ◽  
Single Session ◽  
Ultrasound Guided ◽  
Hifu Ablation ◽  
Hyperintense Signal ◽  
Significant Difference

PurposeThis study aimed to compare the dose and effectiveness of ultrasound-guided high-intensity focused ultrasound (USgHIFU) ablation of uterine fibroids with different sizes and explore the effect of uterine fibroid size on dose, which provided dose evaluation for clinicians in accordance with the size of uterine fibroids.Materials and MethodsA total of 1,000 patients with symptomatic uterine fibroids who received a single-session USgHIFU treatment were enrolled in this study. The size of fibroids was divided into seven groups: 3–4 cm, 4–5 cm, 5–6 cm, 6–7 cm, 7–8 cm, 8–9 cm, and 9–11 cm. The dose was expressed on the basis of the energy efficiency factor (EEF) as the energy required for ablation per unit volume of tissue, and the non-perfused volume ratio (NPVR) was used to assess the effect of HIFU ablation.ResultsThe median NPVR of 88.3% (IQR: 80.3%–94.8%) was obtained, and no significant difference was observed among the seven groups. The classification of T2-weighted image signal intensity fibroids in the 4–5 cm group was compared with that in the 6–7 cm and 8–9 cm groups, and the difference was significant (p &lt; 0.05). However, the proportion of T2WI hyperintense signal fibroids had no significant difference among the seven groups (p &gt; 0.05). The median EEF was 3.88 J/mm3, and a significant difference was observed among the seven groups of EEF (p &lt; 0.05). The EEF of groups with a fibroid size less than 6 cm was more than double the EEF of groups with a fibroid size above 6 cm. In addition, the EEF of groups with a fibroid size of 4–5 cm and 3–4 cm was 3–4 times higher than those with a fibroid size above 7 cm (p &lt; 0.05).ConclusionsA single-session HIFU ablation for uterine fibroids of 3–11 cm can obtain an NPVR of more than 80%. The EEF decreased with the increase of the size of uterine fibroids. A fibroid size of 6.5 cm was considered as a clinical meaningful point affecting EEF.

Building cyberinfrastructure systems to support integrative, macroscale analyses of sedimentary ancient DNA records: current resources, needs, and opportunities

2021 ◽  
Author(s):  
John Williams ◽  
Keyword(s):  
Ancient Dna ◽  
Unmet Need ◽  
Taxonomic Resolution ◽  
Joint Analysis ◽  
Sequence Variant ◽  
Bioinformatics Pipeline ◽  
Widespread Application ◽  
Sedimentary Environments ◽  
Taxonomic Groups ◽  
Analytical Approaches

&lt;p&gt;The number and extent of ancient DNA records from sedimentary environments (sedaDNA) is rapidly increasing, which creates new opportunities for integrative and macroscale investigations into past population, community, and environmental dynamics at unprecedented taxonomic resolution and spatiotemporal extent.&amp;#160; However, fully achieving this potential requires a robust cyberinfrastructure that supports the joint analysis of many sedaDNA records with each other and with genomic reference libraries, the latest geochronological controls and age-depth models, complementary paleoecological and paleoenvironmental proxies, and the most recent and updated DNA reference library for taxonomic identifications.&amp;#160; Any cyberinfrastructure for macroscale data synthesis must address the variety of ancient DNA records (e.g. taxonomic groups, analytical approaches, depositional contexts) and leverage existing resources and standards such as the Neotoma Paleoecology Database, the MGnify and MG-RAST resources for environmental genomics, and the MixS standard for genetic sequences.&amp;#160; In response, a Cyberinfrastructure for Ancient Sedimentary DNA working group has been meeting regularly since summer 2020 to assess the current state of science and informatics, assess needs and gaps, and establish recommendations for next steps forward.&amp;#160; An initial survey found over 420&amp;#160; sites worldwide with published or in-development sedaDNA records, with greatest densities in Eurasia.&amp;#160; Metabarcoding records, including Amplicon Sequence Variant data and derived taxonomic inferences, are a top priority for trial uploads to Neotoma, with pilot uploads underway, because of the relatively small dataset volumes, the widespread application of metabarcoding assays, and potential of integrating these records with other paleoecological data holdings in Neotoma and linked paleodata resources such as Linked Earth and paleoclimatic data at NOAA&amp;#8217;s National Centers for Environmental Informatics.&amp;#160; Because taxonomic inferences are heavily conditioned by choice of bioinformatics pipeline and reference databases, a major unmet need is a repository for minimally processed output from raw sequences.&amp;#160; In general, no existing genomics or paleoecological resource meets all needs of the sedaDNA community, although each covers key elements, so there is a good potential of advancing macroscale data syntheses by leveraging and linking existing resources.&lt;/p&gt;

Sign in / Sign up

Export Citation Format

Share Document