scholarly journals ES KRIM KEFIR LABU KUNING ANALYSIS OF ACCEPTANCE LEVEL , TOTAL PLATE COUNT, BETA-CAROTEN, AND ANTIOXIDANT IN PUMPKIN KEFIR ICE CREAM

2019 ◽  
Vol 11 (1) ◽  
pp. 131
Author(s):  
Amanda Kania Diandini ◽  
Mulus Gumilar ◽  
Gurid Ekomulyo ◽  
Fred Agung ◽  
Hanifa Zakiah Muslimah
Keyword(s):  
Diabetes Mellitus ◽  
Skim Milk ◽  
Ice Cream ◽  
Beta Carotene ◽  
Plate Count ◽  
Artificial Sweetener ◽  
Total Plate Count ◽  
Dpph Method ◽  
Plate Count Method

The safe ice cream which is consumed by Diabetes Mellitus sufferers is made by subtituting skim milk, cream and sugar with kefir, pure pumpkin, cornstarch, vegetable oil, and artificial sweetener special gor Diabetes Mellitus. The aim of this research is knowing predilection level test to ice cream pumpkin kefir, microbes total, beta-caroten levels, and antioxidant levels. This research is conducted with experimental method. The research method used hedonic test to determine the level of preference, total plate count method to determine the number of microbes, UV-Vis spectrophotometry method for testing beta-carotene levels, and DPPH method for testing antioxidant levels. The results of the Kruskal Wallis test showed differences in the level of texture preference and there were no differences that appreciated the level of taste, color, and aroma. Ice cream pumpkin kefir that is liked most are from texture, taste, color and aroma side exists in balance F2 with the ratio of kefir and pumpkin 70%:30%. Suggestions for further research need to be concerned about the procedure for making pumpkin kefir ice cream in order to obtain the number of microbes according to the standard probiotic products, higher levels of beta-carotene and antioxidants and further research on kefir pumpkin ice cream research for Type 2 Diabetes Mellitus patients.

Uji Kesukaan Es Krim Kefir Labu Kuning

2017 ◽  
Vol 9 (1) ◽  
pp. 16
Author(s):  
Amanda Kania Diandini
Keyword(s):  
Diabetes Mellitus ◽  
Data Analysis ◽  
Experimental Method ◽  
Skim Milk ◽  
Ice Cream ◽  
Artificial Sweetener ◽  
Value Analysis ◽  
Nutrient Value ◽  
Bioactive Content ◽  
Price Analysis

The safe ice cream which is consumed by Diabetes Mellitus sufferers is made by subtituting skim milk, cream and sugar with kefir, pure pumpkin, cornstarch, vegetable oil, and artificial sweetener special gor Diabetes Mellitus. Kefir is known can decrease (blood sugar) because of its bioactive content. The aim of this research is knowing predilection level test to ice cream pumpkin kefir. This research is conducted with experimental method. The data analysis includes predilection test, nutrient value analysis, and price analysis. Ice cream pumpkin kefir that is liked most are from texture side, the cheapest price, and also the highest fiber content exists in balance 578 with the ratio of kefir and pumpkin 50%:50%. Ice cream pumpkin kefir that is liked most from colour side exists in balance 236 with the ratio of kefir and pure pumpkin 70%:30%. Ice cream pumpkin kefir that is liked most from taste side and aroma exists in balance 522 with the ratio between kefir and pure pumpkin 80%:20%.

Pengaruh Suhu Penyimpanan terhadap Organoleptik, Derajat Keasaman dan Pertumbuhan Bakteri Coliform pada Susu Pasteurisasi

2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan
Keyword(s):  
Storage Temperature ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Initial Analysis ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Pathogenic Microbes ◽  
Total Coliform Bacteria

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi

scholarly journals Reduction of Microbe Contamination through Steaming Process to Cocoa Beans Using Steaming Chamber

2014 ◽  
Vol 30 (1) ◽  
Author(s):  
Hendy Firmanto
Keyword(s):  
Microbial Contamination ◽  
Microbial Population ◽  
Plate Count ◽  
Cocoa Bean ◽  
Contamination Level ◽  
Cocoa Beans ◽  
Total Plate Count ◽  
Microbial Analysis ◽  
Plate Count Method ◽  
Nutrient Changes

Dry cocoa bean quality is also determined by its microbe contamination level. Steaming process for dried cocoa beans as a pretreatment process was selected because of less effect on organic compound inside the dried cocoa bean. This experiment aim was to study microbial contamination level of cocoa beans using steaming process, determining its microbial population and evaluate its chemical changes. Experiment was carried out in Postharvest Laboratory of Indonesian Coffee and Cocoa Research Institute. Cocoa beans for the experiment were lots collected from four farms in Jayapura, Papua with different microbial contamination level for each lot. Results of this experiment showed that optimum steaming process was 15 minutes at 100 O C with 10 minutes preheating time. Microbial analysis result of the four lots after complete steaming process by total plate count method showed the same result (<3.0 x 103 cfu). Most of the decrease in microbial contaminant appeared in the plate was 73.5% of Staphylococcus aureusand 0.058% of Penicilliumsp. Bean acidity (pH) after steaming increased (4.76 to 4.80) and free fatty acid increased (1.81% to 1.96%) while carbohydrate content decreased (17.5% to 15.9%) and as well as protein content (12.6% into 11.7%). Key words: cocoa bean, steaming process, microbe reduction, nutrient changes

scholarly journals Effect of temperature and duration of thermal pasteurization on polyphenol oxidase (PPO) enzyme activity, total plate count (TPC), physicochemical and organoleptic properties of Cavendish banana fruit juice (Musa cavendishii)

2021 ◽  
Vol 4 (2) ◽  
pp. 117-130
Author(s):  
Bambang Dwi Argo ◽  
Cici Damayanti ◽  
Anik Wulandari ◽  
Umi Mianadhiroh
Keyword(s):  
Enzyme Activity ◽  
Fruit Juice ◽  
Effect Of Temperature ◽  
Plate Count ◽  
Banana Fruit ◽  
Cavendish Banana ◽  
Total Plate Count ◽  
Banana Juice ◽  
Dpph Method ◽  
Thermal Pasteurization

This research aims to determine the effect of temperature and duration of thermal pasteurization process in Cavendish banana juice. Cavendish banana is an abundant cultivated banana that has not been used properly, which is a climacteric fruit that has increased ethylene production after harvesting. Cavendish bananas have great potential as fruit juice. Pasteurization was carried out at temperatures of 55, 60, 65, 70 and 75 ℃ for 5, 10, and 15 minutes. Pasteurization with higher temperature and time can increase the total dissolved sugar and viscosity as well as decrease the pH value and PPO enzyme activity. The reducing sugar content of Cavendish banana juice was determined using the DNSA method with the greatest value at 55 ℃ for 5 minutes, which is 9.53%. Meanwhile, the content of phenol and flavonoid was determined using the Krishnan and Sinija method. The highest value of phenol and flavonoid content at 60 ℃ for 5 minutes, was 0.1728 mg GAE/g and 0.1583 mg QE/g sample. Antioxidant activity was determined using the DPPH method and the greatest percentage of inhibition at 60 ℃ for 5 minutes, which is 61.60%. Vitamin C levels were identified by the UV-Vis spectrophotometry method with the greatest value at 55 ℃ for 5 minutes, which is 1.1409 mg /100 g. The Total Plate Count (TPC) of Cavendish banana juice decreased after pasteurization with the smallest total number of bacteria at 75 ℃ for 15 minutes, with a value of 3.2×101 CFU/ml. Pasteurization process at 75 ℃ for 15 minutes can reduce the activity of PPO enzyme as much as 97%. In the organoleptic test, preferred level of Cavendish banana juice to all attributes of the average hedonic scale with the category of the likes.

scholarly journals Effects of contact time of natural cu-zeolite on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa

2020 ◽  
Vol 6 (1) ◽  
pp. 25
Author(s):  
Erwid Fatchur Rahman ◽  
Bambang Dwirahardjo ◽  
Poerwati Soetji Rahajoe
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Bacterial Growth ◽  
Contact Time ◽  
Plate Count ◽  
Average Growth ◽  
30Th Minute ◽  
Total Plate Count ◽  
Randomized Design ◽  
Plate Count Method

Infection of a surgical wound due to bacteria is a major problem for surgical patients. Cu-zeolite is a material that can suppress bacterial growth with reversible cation characteristics and adsorption to be developed into non-toxic disinfectants for humans. Packaging uses filter paper to keep disinfectant solutions or instruments that will be sterilized clean. This study aimed to observe the effects of contact time of natural Cu-zeolite on the growth of Streptococcus pyogenes (S. pyogenes) and Pseudomonas aeruginosa (P. aeruginosa) bacteria. An experimental research was simple randomized design. Cu-zeolite 10 grams were packaged in Whatman no 42 paper bags measuring 5 x 5 cm2, contacted for 15, 30 and 45 minutes in 99.5 ml of distilled water exposed to 0.5 x 108 CFU / ml of S. pyogenes and P. aeruginosa. Subsequently, bacterial growth was calculated using total plate count method. The average growth of S.pyogenes for 15, 30 and 45 minutes (1840 ± 571.236 CFU; 29 ± 16.33 CFU and 0 CFU) while P. aeruginosa was (2776 ± 725.277 CFU; 55 ± 23.214 CFU and 0 CFU) respectively. Based on the independent t-test on Cu-zeolite, the bacterial growth in the 15th and 30th minute contact between S. pyogenes and P. aeruginosa was significantly different (pth and 30th minute contact.

scholarly journals Komunitas Bakteri pada Saluran Pencernaan Lobster Mutiara (Panulirus ornatus)

2019 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Arba’i Kartini Nurhasanah ◽  
Faturrahman Faturrahman
Keyword(s):  
Digestive Tract ◽  
Sea Water ◽  
Plate Count ◽  
Intestinal Cells ◽  
Bacterial Cells ◽  
Bacterial Isolates ◽  
Water Complex ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Total Bacteria

The presence of microbial communities in the digestive tract affects the physiological functions of digestion. The purpose of this study is to determine the bacterial community in the digestive tract of lobsters, especially pearl lobsters. This research is descriptive explorative. Samples were taken from the lobster digestive tract which was divided into 3, namely the cardiac, pylorus and intestinal sections and each part was counted for the total bacteria using the TPC (Total Plate Count) method. Then isolated from the Sea Water Complex media and partially characterized. The results showed that the total number of bacterial cells in cardiac, piloric and intestinal cells were 41.91 x 108 cfu.ml-1, 18.26 x 108 cfu.ml-1 and 14.10 x 108 cfu.ml-1 respectively. The number of bacterial isolates obtained from the lobster pearl digestive tract were 51 isolates with details of 18 cardiac isolates, 16 isolates in piloric, and 17 isolates in the intestine. Phenotypic isolates obtained from lobster digestive tract bacteria consisted of monococcus 37.25%, diplococcus 27.45%, streptococcus 29.41%, staphylococcus 1.96%, and long baccil 3.92%, while gram positive 62.65 % and gram negative at 37.25%

scholarly journals PENGARUH AIPengaruh Air Rebusan terhadap Kualitas Ikan Kembung Rebus (Rastrelliger sp.)aR REBUSAN TERHADAP KUALITAS IKAN GEMBUNG REBUS (Rastrelliger sp)

2019 ◽  
Vol 1 (1) ◽  
pp. 7-11
Author(s):  
M.Yusri Dadan Nugraha ◽  
Riyanto Riyanto ◽  
Hanifah Mutia Z.N Amrul
Keyword(s):  
Qualitative Method ◽  
Plate Count ◽  
Fish Production ◽  
Total Plate Count ◽  
Count Method ◽  
Plate Count Method ◽  
Boiled Water ◽  
Descriptive Qualitative

The research on bacterial testing on boiled water of boiled mackerel (Rastelliger sp) has been conducted. The study applied treatment of boiled water that is not replaced and is used continuously in several stages. This study aims to determine the effect of boiled water on the quality of fish production, and determine its effect on meat tectrus, gill color, and aromas. The study was conducted with a descriptive qualitative method. Boiled mackerel quality is measured using the total plate count method, which is to count the number of bacterial colonies in the water before boiling and after boiling.

scholarly journals POTENSI INFUSA DAUN NANGKA SEBAGAI OBAT KUMUR HERBAL

2021 ◽  
Vol 26 (1) ◽  
pp. 17-23
Author(s):  
Christ Alfianus Tosubu ◽  
Nunung Sulistyani ◽  
Nur Khikmah
Keyword(s):  
Oral Cavity ◽  
Colony Growth ◽  
Plate Count ◽  
Bacterial Colony ◽  
Artocarpus Heterophyllus ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Before And After ◽  
Plate Count Method ◽  
The Difference

Penelitian ini dilakukan untuk mengkaji potensi daun nangka (Artocarpus heterophyllus) sebagai obat kumur herbal dengan melihat jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Daun nangka berwarna hijau muda yang diperoleh dari Babadan, Banguntapan, Bantul dibuat simplisia. Penentuan potensi dilakukan dengan menentukan perbedaan jumlah pertumbuhan koloni bakteri rongga mulut sebelum dan sesudah berkumur dengan infusa daun nangka. Uji potensi infusa daun nangka sebagai obat kumur dilakukan dengan menghitung perbedaan jumlah koloni bakteri rongga yang diperoleh dengan melakukan swab pada pangkal lidah sebelum dan sesudah berkumur. Perhitungan koloni bakteri dilakukan menggunakan metode hitung cawan (total plate count) pada media plate count agar (PCA). Hasil penelitian menunjukkan rata-rata jumlah koloni bakteri sebelum dan sesudah berkumur dengan infusa daun nangka yaitu 1,78 x 107 CFU/mL dan 7,71 x 106 CFU/mL. Perlakuan pemberian obat kumur infusa daun nangka secara signifikan mampu menurunkan jumlah koloni bakteri rongga mulut. Infusa daun nangka berpotensi sebagai alternatif obat kumur herbal. THE POTENCY OF JACKFRUIT LEAF INFUSION AS A HERBAL MOUTHWASHThis study was conducted to examine the potential of jackfruit (Artocarpus heterophyllus) leaves as a herbal mouthwash by identifying the growth of bacterial colonies in the oral cavity before and after gargling with jackfruit leaf infusion. The light green jackfruit leaves obtained from Babadan, Banguntapan, Bantul were made simplicia. The determination of potency was done by determining the difference in the number of bacterial colony growth in the oral cavity before and after gargling with jackfruit leaf infusion. The potential test of jackfruit leaf infusion as a mouthwash was carried out by calculating the difference in the number of cavity bacterial colonies obtained by swab at the base of the tongue before and after gargling. Bacterial colonies were counted using the total plate count method on plate count agar (PCA) media. The results showed that the average number of bacterial colonies before and after rinsing with jackfruit leaf infusion was 1.78 x 107 CFU/mL and 7.71 x 106 CFU/mL. The treatment of giving jackfruit leaf infusion mouthwash was significantly able to reduce the number of bacterial colonies in the oral cavity. Jackfruit leaf infusion has the potential as an alternative to herbal mouthwash.

scholarly journals The Effect of Turmeric Extract (Curcuma Domestica Val) Against the Durability of Yellow Rice Storage

2019 ◽  
Vol 2 (1) ◽  
pp. 30-36
Author(s):  
Hetty Lendora Maha ◽  
Erly Sitompul ◽  
Jansen Silalahi
Keyword(s):  
Antibacterial Activity ◽  
Shelf Life ◽  
Microbial Growth ◽  
Plate Count ◽  
Traditional Food ◽  
Extract Concentration ◽  
Cooked Rice ◽  
Total Plate Count ◽  
Turmeric Extract ◽  
Plate Count Method

Yellow cooked rice is a traditional food in some regions in Indonesia. Yellow cooked rice with the addition of turmeric and flavoring which can longer shelf life of savings because it has antibacterial activity and flavor. The purpose of this study was to determine the effect of turmeric extract (Curcuma domestica Val) to extend shelf life of yellow cooked rice. The samples used were made of yellow cooked rice with handmade and without any seasoning flavored with turmeric extract concentration of 1.8% and 2.4%. Organoleptic testing were conducted every 3 hours, test of microbial growth daily and inhibition of turmeric extract with a concentration of 1.8% and 2.4% of fungi isolated from yellow cooked rice with a total plate count method. Research showed that turmeric extract concentration and the addition of  herbs affect the longer shelf life of yellow cooked rice store. High more and more  concentration of the turmeric extract can made longer the shelf life of yellow cooked rice either through organoleptic and also total plate count. Yellow cooked rice without seasoning with turmeric extract concentration of 1.8% and 2.4% were 30 and 33 hours respectively, while the yellow cooked rice was flavored with the same concentration of turmeric extract  were 36 and 39 hours respectively.

Sign in / Sign up

Export Citation Format

Share Document