scholarly journals Komunitas Bakteri pada Saluran Pencernaan Lobster Mutiara (Panulirus ornatus)

2019 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Arba’i Kartini Nurhasanah ◽  
Faturrahman Faturrahman

The presence of microbial communities in the digestive tract affects the physiological functions of digestion. The purpose of this study is to determine the bacterial community in the digestive tract of lobsters, especially pearl lobsters. This research is descriptive explorative. Samples were taken from the lobster digestive tract which was divided into 3, namely the cardiac, pylorus and intestinal sections and each part was counted for the total bacteria using the TPC (Total Plate Count) method. Then isolated from the Sea Water Complex media and partially characterized. The results showed that the total number of bacterial cells in cardiac, piloric and intestinal cells were 41.91 x 108 cfu.ml-1, 18.26 x 108 cfu.ml-1 and 14.10 x 108 cfu.ml-1 respectively. The number of bacterial isolates obtained from the lobster pearl digestive tract were 51 isolates with details of 18 cardiac isolates, 16 isolates in piloric, and 17 isolates in the intestine. Phenotypic isolates obtained from lobster digestive tract bacteria consisted of monococcus 37.25%, diplococcus 27.45%, streptococcus 29.41%, staphylococcus 1.96%, and long baccil 3.92%, while gram positive 62.65 % and gram negative at 37.25%

2017 ◽  
Vol 4 (2) ◽  
pp. 87-91
Author(s):  
Ekamaida Ekamaida

The soil fertility aspect is characterized by the good biological properties of the soil. One important element of the soil biological properties is the bacterial population present in it. This research was conducted in the laboratory of Microbiology University of Malikussaleh in the May until June 2016. This study aims to determine the number of bacterial populations in soil organic and inorganic so that can be used as an indicator to know the level of soil fertility. Data analysis was done by T-Test that is by comparing the mean of observation parameter to each soil sample. The sampling method used is a composite method, which combines 9 of soil samples taken from 9 sample points on the same plot diagonally both on organic soil and inorganic soil. The results showed the highest bacterial population was found in total organic soil cfu 180500000 and total inorganic soil cfu 62.500.000


2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi


Author(s):  
Hendy Firmanto

Dry cocoa bean quality is also determined by its microbe contamination level. Steaming process for dried cocoa beans as a pretreatment process was selected because of less effect on organic compound inside the dried cocoa bean. This experiment aim was to study microbial contamination level of cocoa beans using steaming process, determining its microbial population and evaluate its chemical changes. Experiment was carried out in Postharvest Laboratory of Indonesian Coffee and Cocoa Research Institute. Cocoa beans for the experiment were lots collected from four farms in Jayapura, Papua with different microbial contamination level for each lot. Results of this experiment showed that optimum steaming process was 15 minutes at 100 O C with 10 minutes preheating time. Microbial analysis result of the four lots after complete steaming process by total plate count method showed the same result (<3.0 x 103 cfu). Most of the decrease in microbial contaminant appeared in the plate was 73.5% of Staphylococcus aureusand 0.058% of Penicilliumsp. Bean acidity (pH) after steaming increased (4.76 to 4.80) and free fatty acid increased (1.81% to 1.96%) while carbohydrate content decreased (17.5% to 15.9%) and as well as protein content (12.6% into 11.7%). Key words: cocoa bean, steaming process, microbe reduction, nutrient changes


Author(s):  
Ruslan A. Daeng ◽  
Azis Husen

This research was conducted to determine the characteristics of Pseudomonas sp. and mold on dried anchovies. The sample in this study was obtained from the fishing community of Toniku Village, West Halmahera Regency, North Maluku Province. The stages carried out in this study were 3 (three) stages, namely the sample preparation stage, the analysis phase and the identification stage of Pseudomonas sp. and mold. The results showed that the characteristics of the Total Plate Count (TPC) of dried anchovy in the Toniku Village obtained a different composition from each other, with the highest log Total Plate Count (TPC) obtained, namely 5.5 or 3.2 x 105 Cfu/g or 320.000 cells bacteria, while the lowest Total Plate Count (TPC) log was obtained, 2.5 or 3.1 x 102 Cfu/g or 310 bacterial cells and for total Pseudomonas bacteria from the three sampling locations no growth was found. The highest total log fungi were obtained ie 5.6 or 3.7 x 105 Cfu/g or 370.000 mold cells, while the lowest total log fungi were obtained which were 2.4 or 2.6 x 102 Cfu/g or 260 mold cells


2020 ◽  
Vol 20 (2) ◽  
pp. 298
Author(s):  
Fahruddin Fahruddin ◽  
Syahruddin Kasim ◽  
Eka Ummi Rahayu

The mining industry in Indonesia is growing rapidly, giving an environmental impact from the waste produced because it contains dangerous heavy metals, one of which is cadmium metal (Cd). The purpose to know the ability of cadmium resistance in bacterial isolates. Bacterial isolates were obtained from soil samples from the Poboya Gold Mine field, Palu. Bacterial isolation and selection were carried out by the plate count method, resistance testing using nutrient broth media containing heavy metals Cd, analysis of Cd concentrations using atomic absorption spectrophotometers. From the results of the study, four types of Cd resistant isolates were obtained based on macroscopic and microscopic characterization results, namely isolates EK1, EK2, EK3 and EK4. The two best isolates of Cd resistance, EK2 and EK4 isolates were tested on media containing 20 ppm Cd, showing that both of these isolates were able to significantly reduce Cd concentrations in the supernatant and pellets.


2015 ◽  
Vol 78 (6) ◽  
pp. 1186-1190 ◽  
Author(s):  
HUBERT ANTOLAK ◽  
DOROTA KREGIEL ◽  
AGATA CZYZOWSKA

The aim of the study was to evaluate the adhesion abilities of the acetic acid bacterium Asaia bogorensis to glass and polystyrene in the presence of American cranberry (Vaccinium macrocarpon) juice. The strain of A. bogorensis used was isolated from spoiled commercial fruit-flavored drinking water. The cranberry juice was analyzed for polyphenols, organic acids, and carbohydrates using high-performance liquid chromatography and liquid chromatography–mass spectrometry techniques. The adhesive abilities of bacterial cells in culture medium supplemented with cranberry juice were determined using luminometry and microscopy. The viability of adhered and planktonic bacterial cells was determined by the plate count method, and the relative adhesion coefficient was calculated. This strain of A. bogorensis was characterized by strong adhesion properties that were dependent upon the type of surface. The highest level of cell adhesion was found on the polystyrene. However, in the presence of 10% cranberry juice, attachment of bacterial cells was three times lower. Chemical analysis of juice revealed the presence of sugars, organic acids, and anthocyanins, which were identified as galactosides, glucosides, and arabinosides of cyanidin and peonidin. A-type proanthocyanidins responsible for the antiadhesion properties of V. macrocarpon also were detected.


2020 ◽  
Vol 3 (1) ◽  
pp. 113
Author(s):  
Sarni Sarni ◽  
Haslianti Haslianti ◽  
Suwarjoyowirayatno Suwarjoyowirayatno

Characteristics of Chemical Composition and Total Bacterial of Roa (Hemirhampus sp.) Smoke Marketed in the Bungku District of Central Morowali DistrictABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, and fat) and total bacteria (TPC) of smoked roa fish. Data analysis this study used descriptive analysis. The method used study is the survey method (Simple Random Sampling). The results of the chemical composition of the highest treatment water content P2 13.02%, ash P3 5.65%, protein P3 40.82%. and fat P3 13.87%. The best organoleptic test results were found in P1 with 7.30 appearance, 8.20 odor, 8.00 taste, 7.80 texture, and the best mushroom specifications were at P2 9.00. The lowest total bacteria test was found with 0,83×105 Log CFU/gr.Keywords: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimia  (kadar air, kadar abu, kadar protein dan kadar lemak) dan total bakteri (TPC) terhadap ikan roa asap. Analisis data yang digunakan dalam penelitian ini yaitu menggunakan analisis deskriptif. Metode yang digunakan dalam penelitian ini yaitu metode survey (Simple Random Sampling). Hasil penelitian komposisi kimia perlakuan tertinggi kadar air P2 13,02%. Kadar abu tertinggi terdapat pada perlakuan P3 5,65%. Kadar protein perlakuan tertinggi terdapat pada P3 40,82%. dan kadar lemak perlakuan tertinggi terdapat pada  P3 13,87%. Hasil penilaian uji organoleptik terbaik terdapat pada P1 sepesifikasi kenampakan 7,30, bau 8,20, rasa 8,00, tekstur 7,80 dan pada spesifikasi jamur nilai terbaik terdapat pada P2 9,00. Total bakteri terendah terdapat pada P3 dengan nilai 0,83×105 Log CFU/gr.Kata kunci: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik


2018 ◽  
Vol 67 ◽  
pp. 04001
Author(s):  
Muhammad Zakaria Umar ◽  
Arman Faslih ◽  
Mazhfia Umar

At this time began to be revived the use of local materials. This is based on aesthetic and economic considerations. Therefore, researchers are encouraged to examine the knowledge of the utilization of local resources, especially nipah leaves. In Petoaha Sub District, Abeli District, Kendari City is located Kampung KB (Family Planning). Kampung KB is a former village of Bajo ethnic landed (formerly living on water). Mothers in Kampung KB are busy making roof material from nipah leaves. The leaves are taken from the palm trees that grow around Kampung KB. The phenomenon in the field that leaves soaked in sea water for 1-3 days before the roof is made. According to them that the leaves are soaked in sea water has a long lifetime compared to leaves that are not soaked in sea water. Based on their experience that watersoaked leaves have a lifetime for 3-4 years, can even reach 6-7 years when the roof thickness reaches 10 layers of palm leaves. Nipah leaves do not soak sea water has age wear for 2-3 years. This research is aimed to test the nipah leaf of marine water and nipah leaves do not soak the sea water against the period of usage. Metode plate count digunakan dalam penelitian ini. The plate count method to test the total microbial between fresh nipah leaf and nipah leaves is soaked in sea water. This study concluded that the bacteria on the nipah leaf soak the sea water is lower. Nipah leaves soaked sea water has a lifetime longer than nipah leaves do not soak sea water made roof material.


2020 ◽  
Vol 6 (1) ◽  
pp. 25
Author(s):  
Erwid Fatchur Rahman ◽  
Bambang Dwirahardjo ◽  
Poerwati Soetji Rahajoe

Infection of a surgical wound due to bacteria is a major problem for surgical patients. Cu-zeolite is a material that can suppress bacterial growth with reversible cation characteristics and adsorption to be developed into non-toxic disinfectants for humans. Packaging uses filter paper to keep disinfectant solutions or instruments that will be sterilized clean. This study aimed to observe the effects of contact time of natural Cu-zeolite on the growth of Streptococcus pyogenes (S. pyogenes) and Pseudomonas aeruginosa (P. aeruginosa) bacteria. An experimental research was simple randomized design. Cu-zeolite 10 grams were packaged in Whatman no 42 paper bags measuring 5 x 5 cm2, contacted for 15, 30 and 45 minutes in 99.5 ml of distilled water exposed to 0.5 x 108 CFU / ml of S. pyogenes and P. aeruginosa. Subsequently, bacterial growth was calculated using total plate count method. The average growth of S.pyogenes for 15, 30 and 45 minutes (1840 ± 571.236 CFU; 29 ± 16.33 CFU and 0 CFU) while P. aeruginosa was (2776 ± 725.277 CFU; 55 ± 23.214 CFU and 0 CFU) respectively. Based on the independent t-test on Cu-zeolite, the bacterial growth in the 15th and 30th minute contact between S. pyogenes and P. aeruginosa was significantly different (pth and 30th minute contact.


2019 ◽  
Vol 1 (1) ◽  
pp. 7-11
Author(s):  
M.Yusri Dadan Nugraha ◽  
Riyanto Riyanto ◽  
Hanifah Mutia Z.N Amrul

The research on bacterial testing on boiled water of boiled mackerel (Rastelliger sp) has been conducted. The study applied treatment of boiled water that is not replaced and is used continuously in several stages. This study aims to determine the effect of boiled water on the quality of fish production, and determine its effect on meat tectrus, gill color, and aromas. The study was conducted with a descriptive qualitative method. Boiled mackerel quality is measured using the total plate count method, which is to count the number of bacterial colonies in the water before boiling and after boiling.


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