scholarly journals Perbandingan Aktivitas Antifungi Ekstrak Etanol 70% Dan 96% Buah Parijoto (Medinilla speciosa) Terhadap Candida albicans

2021 ◽  
Vol 3 (1) ◽  
pp. 32-41
Author(s):  
Puji Astutik ◽  
Richa Yuswantina ◽  
Rissa Laila Vifta
Keyword(s):  
Candida Albicans ◽  
Ethanol Extract ◽  
T Test ◽  
Diffusion Method ◽  
P Value ◽  
Fruit Extract ◽  
Fungal Activity ◽  
Activity Test ◽  
Ethanol Ethanol ◽  
Anti Fungal

Parijoto fruit (Medinilla speciosa) contains active compounds of flavonoids, tannins, saponins, glycosides. Parijoto fruit (Medinilla speciosa) has many benefits for human health, one of which is as an anti-fungal. In this study, the antifungal activity test of 70% ethanol extract and 96% ethanol extract of parijoto fruit (Medinilla speciosa) was tested against Candida albicans. This research was conducted by maceration using 70% ethanol and 96% ethanol as solvents. While the anti-fungal activity used the disc diffusion method using a concentration variation of 2.5% w / v; 5% w / v and 10% w / v using the ratio of ketoconazole antibiotics. Parijoto fruit extract (Medinilla speciosa) obtained 70% (%) ethanol and 96% (%) ethanol. Ethanol 70% with a concentration of 2.5% w / v of 25.83 mm; 5% w / v was 27.03 mm and 10% w / v was 28.03 mm, while the ethanol extract of 96% parijoto fruit (Medinilla speciosa) at a concentration of 2.5% w / v was 31.59 mm; 5% w / v of 33.24 mm and 10% w / v of 36.11 mm. The statistical results of 70% ethanol and 96% ethanol, both of which have anti-fungal activity, are not much different, as evidenced by the T-Test statistical test with a P-Value of 0.00 <0.05, because the effect of the 96% parijoto fruit compound is more effective. . The 70% ethanol extract and 96% ethanol extract of parijoto fruit (Medinilla speciosa) can inhibit the growth of Candda albicans with a concentration of 10%. ABSTRAK Buah Parijoto (Medinilla speciosa) mengandung senyawa aktif flavonoid, tanin, saponin, glikosida. Buah parijoto (Medinilla speciosa) merupakan yang memiliki banyak manfaat bagi kesehatan manusia, salah satunya adalah sebagai antifungi. Dalam penelitian ini, uji aktivitas antifungi dari ekstrak etanol 70% dan ekstrak etanol 96% buah parijoto (Medinilla speciosa) akan diuji terhadap Candida albicans. Penelitian ini dilakukan dengan maserasi menggunakan pelarut etanol 70% dan etanol 96%. Sedangkan aktivitas antifungi menggunakan metode difusi cakram menggunakan variasi kosentrasi 2,5% b/v; 5% b/v dan 10% b/v dengan menggunakan perbandingan antibiotik ketokonazole. Ekstrak buah parijoto (Medinilla speciosa) diperoleh hasil etanol 70% (%) dan etanol 96% (%). Etanol 70% dengan konsentrasi 2,5% b/v sebesar 25,83 mm; 5% b/v sebesar 27,03 mm dan 10% b/v sebesar 28,03 mm sedangkan ekstrak etanol 96% buah parijoto (Medinilla speciosa) pada konsentrasi 2,5% b/v sebesar 31,59 mm; 5% b/v sebesar 33,24 mm dan 10% b/v sebesar 36,11 mm. Hasil statistik etanol 70% dan etanol 96% aktivitas antifungi keduanya memiliki aktivitas antifungi yang tidak jauh berbeda sebagaimana dibuktikan dari uji statistik T-Test dengan nilai P-Value 0,00 <0,05, karena pengaruh dari senyawa buah parijoto 96% lebih efektif. Ekstrak etanol 70% dan etanol 96% buah parijoto (Medinilla speciosa) dapat menghambat pertumbuhan Candda albicans dengan kosentrasi 10%.

scholarly journals The Antifungal Effect of WHO Hand Sanitizer to Candida Albicans in Improving Personal Hygiene

2020 ◽  
Vol 1 (2) ◽  
pp. 7
Author(s):  
Dyah Widiastuti ◽  
Ihda Zuyina Zuyina Ratna Sari ◽  
Isya Fikria Kalimah ◽  
Endang Setiani
Keyword(s):  
Candida Albicans ◽  
Inhibition Zone ◽  
World Health ◽  
Personal Hygiene ◽  
Diffusion Method ◽  
Zone Formation ◽  
Hand Sanitizer ◽  
Fungal Activity ◽  
Significant Difference ◽  
Anti Fungal

Hand hygiene is one of the important factors that determine a person's health status. Hands are easily contaminated by microbes from the environment such as bacteria, viruses, and fungi through direct contact. Hand sanitizer is a type of media that can be used to clean hands from disease-causing microbes other than soap. Hand sanitizers are widely used by the community because they are considered more practical to use. This study aims to determine the anti-fungal activity of the hand sanitizer recommended by the World Health Organization (WHO) against Candida albicans. Anti-fungal activity was tested using the well diffusion method. The results showed that the higher the concentration of the hand sanitizer, the bigger the inhibition zone formed. The statistical test results obtained a significance value of p <0.05 at the variation of the concentration of hand sanitizer 50%, 75%, 100%, and treatment control. These results indicate that there is a significant difference between the hand sanitizer treatment and control of the inhibition zone formation in C. albicans. The WHO recommended hand sanitizer has medium inhibitory power against C. albicans bacteria.

scholarly journals Evaluation of Anti Microbial and Anti Fungal Activity of Acalyphaindica L., Leaf Extract

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Sudhakar Chekuri ◽  
Arun Jyoti B ◽  
Saraswathi JSompaga ◽  
Shivaprasadi Panjala ◽  
Roja Ran Anupalli
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fungal Activity ◽  
Bacteria And Fungi ◽  
Anti Fungal ◽  
Acalypha Indica

Anti-microbial and anti-fungal activity of different solvent extracts of Acalypha indica (Euphorbeace family) was tested against bacterial pathogens (Pseudomonasaeruginosa, E.Coli, KlebsiellaPneumonia and Staphylococcus aureus) and fungal strains (Candida albicans, Aspergillus niger, Candida tropicalis andCandida kefyr) using the Agar Well diffusion method . It was observed that all the extracts showed positive activity) against bacteria and fungi. Ethanolic extract of Acalypha indica showed more potency against Staphylococcus aureus with an inhibition zone of12.46 (mm) and Methanolic extract exhibited higher activity against E.coli with an inhibition zone of11.26 (mm). Ethanolic extract of Acalypha indica showed prominent antifungal activity against candida albicans with an inhibition diameter of 12.53 (mm) and Aspergillus niger with a diameter of 9.21 (mm) when compared to other solvent extracts. Erythromycin and Ketoconazole were used as positive standards for antimicrobial and anti fungal experiments. In the present study, Ethanol extract showed a varying degree of inhibition to the growth of tested organisms compared to Methanol, Acetone and Chloroform against Bacteria and Fungi. The results confirmed the presence of antibacterial and antifungal compounds in shade dried extracts of Acalypha indica against human pathogenic organisms.

IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS EKSTRAK ETANOL HERBA MENIRAN (Phyllanthus niruri L.) TERHADAP CANDIDA ALBICANS MENGGUNAKAN METODE DIFUSI CAKRAM

2017 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Anita Apriliana ◽  
Eko Kusumawati ◽  
Selvitawati Selvitawati
Keyword(s):  
Candida Albicans ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
P Value ◽  
Phyllanthus Niruri ◽  
Significant Difference ◽  
Agar Media ◽  
One Way Anova

The aim of this research is to know the ability of meniran ethanol extract (Phyllanthus niruri L.) in inhibiting the growth of Candida albicans. The research design is an experimental study. The extract used was herbal meniran extract made by maceration using 70% ethanol solvent, the content of ethanol extract of meniran tested against Candida albicans using disc diffusion method were 5%, 10%, 20%, 30% and 40%. Activity test conducted on Sabaraound Dextrose agar media that has been smeared with Candida albicans culture which has been standardized with Mc Farland standard. The pre-tested disc paper is first dipped into the extract with various concentrations. Incubated at 30 ° C for 24 hours and the inhibit zone formed was measured. The result showed that herbal ethanol extract of meniran resulted in drag zone diameter at Candida albicans ie 8,5 mm; 10.3 mm; 12.6 mm; 14.1 mm and 14.3 mm, for negative control 0 mm and positive control 20.1 mm. Data obtained from the results of the study were analyzed using one way anova method showed p - value <0,05, it was concluded that there was significant difference between negative control, 5%, 10%, 20%, 30%, 40% and positive control.

scholarly journals Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans

WARTA FARMASI ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 71-80
Author(s):  
Ummu Kalsum ◽  
Ayu Ayu
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Daucus Carota ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Daucus Carota L ◽  
Zone Diameter ◽  
Extract Concentration ◽  
Activity Test ◽  
Agar Diffusion Method

ABSTRAK Telah dilakukan penelitian Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol umbi wortel sebagai antifungi terhadap pertumbuhan Candida albicans dengan menggunakan variasi konsentrasi yang berbeda yaitu 1% b/v, 3% b/v dan 5% b/v. Ekstrak etanol umbi wortel diperoleh dengan cara maserasi menggunakan pelarut etanol 70%. Pengujian aktivitas antifungi menggunakan metode difusi agar dengan cara sumuran untuk mengetahui aktivitas antifungi dengan mengamati daerah hambatan. Hasil penelitian ini menunjukkan bahwa konsentrasi ekstrak 1% b/v, 3% b/v dan 5% b/v dapat memberikan aktivitas yang menghambat pertumbuhan jamur uji. Terdapat penambahan diameter zona hambat pada setiap kenaikan konsentrasi yaitu 1% b/v (15,7 mm), 3% b/v (18,9 mm), dan 5% b/v (19,8 mm) terhadap jamur Candida albicans. Semua variasi konsentrasi ekstrak memiliki aktivitas antifungi yang kuat dalam menghambat pertumbuhan Candida albicans. Kata kunci: Ekstrak, Daucus carota L., Antifungi, Candida albicans ABSTRACT It has conducted a study of activity test of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans. This the study aims at knowing the activity of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans by using different variance concentration they are 1% b/v, 3% b/v and 5% b/v. Carrot tuber ethanol the extract was obtained by maceration by using 70% of ethanol dissolver. Activity test of antifungal by using agar diffusion method with a well method to find out the the activity of antifungal by observing the obstruction zone. The result of this research shows that concentration extract 1% b/v, 3% b/v and 5% b/v can give activity which impedes the growth of fungal test. There is increasing of obstruction zone diameter in every increment of of the concentration they are 1% b/v (15,7 mm), 3% b/v (18,9 mm) and 5% b/v (19,8mm) toward Candida albicans fungi. All extract concentration variation has a strong antifungal activity in impeding the growth of Candida albicans.

scholarly journals FORMULASI SEDIAAN SABUN CAIR EKSTRAK ETANOL DAUN TURI (Sesbania grandiflora L.) DAN UJI ANTIJAMUR TERHADAP Candida albicans

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 334
Author(s):  
Mariando N. Ering ◽  
Paulina V. Y. Yamlean ◽  
Irma Antasionasti
Keyword(s):  
Candida Albicans ◽  
Ethanol Extract ◽  
Experimental Methods ◽  
Diffusion Method ◽  
Liquid Soap ◽  
Sesbania Grandiflora ◽  
Weight Test ◽  
Rate Test ◽  
Anti Fungal ◽  
Fungus Growth

ABSTRACTThe leaves of Hummingbird (Sesbania grandiflora L.) contain flavonoid compounds, saponins and tannins that are antifungal. This research aims to formulate liquid soap extracts of Hummingbird leaf ethanol and test the effectiveness of the antifungal preparations of the liquid soap of Hummingbird leaf ethanol with a concentration of 4%, 6%, 8%, 10% and 12% of the fungus growth of Candida albicans. This research uses experimental methods. The formulation of this liquid soap of Hummingbird leaf ethanol is conducted organoleptic test, pH, high foam, moisture content, free alkaline content, and type weights. Testing the effectiveness of antifungal to Candida albicans growth is done by diffusion method. Liquid soap preparations of Hummingbird leaf ethanol extract meet the standards set by SNI for organoleptic testing, pH test, Foam High test, water content test, free alkali rate test, and type weight test. Based on the anti-fungal testing of the preparation of a liquid soap extract of Hummingbird leaf ethanol to the fungus Candida albicans obtained the results of all preparations have a resistance to fungi and strong categorized.Keywords:  Hummingbird leaves, Liquid soap, AntifungalABSTRAK Daun Turi (Sesbania grandiflora L.) memiliki kandungan senyawa flavonoid, saponin dan tannin yang bersifat sebagai antijamur. Penelitian ini bertujuan untuk memformulasikan sabun cair ekstrak etanol daun Turi dan menguji efektivitas antijamur sediaan sabun cair ekstrak etanol daun Turi dengan konsentrasi 4%, 6%, 8%, 10% dan 12% terhadap pertumbuhan jamur Candida albicans. Penelitian ini menggunakan metode eksperimental. Formulasi sediaan sabun cair ekstrak etanol daun Turi ini dilakukan pengujian organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, dan bobot jenis. Pengujian efektivitas antijamur terhadap pertumbuhan Candida albicans dilakukan dengan metode difusi. Sediaan sabun cair ekstrak etanol daun turi memenuhi standar yang ditetapkan SNI pada pengujian organoleptik, uji pH, uji tinggi busa, uji kadar air, uji kadar alkali bebas, dan uji bobot jenis. Berdasarkan pengujian antijamur sediaan sabun cair ekstrak etanol daun Turi terhadap jamur Candida albicans didapatkan hasil semua sediaan memiliki daya hambat terhadap jamur dan dikategorikan kuat.Kata Kunci: Daun Turi, Sabun Cair, Antijamur

scholarly journals ANTI-MICROBIAL ACTIVITY OF GINGER FLOWER AGAINST SOME CAUSATIVE AGENT OF ACUTE RESPIRATORY INFECTION

2021 ◽  
Vol 7 (1) ◽  
pp. 15-20
Author(s):  
Arni Marlinda Zai ◽  
Suandy Suandy ◽  
Andrico Napolin Lumban Tobing
Keyword(s):  
Candida Albicans ◽  
Microbial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
P Value ◽  
Haemophilus Influenza ◽  
Streptococcus Pneumonia ◽  
Flower Extract ◽  
Significant Difference

Various microorganisms such as Streptococcus pneumonia, Haemophilus influenza, Chlamydia spp, and Mycoplasma pneumonia can cause ARI. The ginger flower which contains various phytochemicals that have potential anti-microbial. Recent study was aimed to explore the anti-microbial properties of ginger flower extract. This study was experimental in vitro study with the disc diffusion method. The ethanol extract of Ginger flowers was obtained by maceration method with a ratio of Simplicia to solvent 1:10. Then it concentrated with a rotary evaporator. The ethanol extract of the ginger flower was diluted by DMSO into four concentrations viz. 100%, 75%, 50%, and 25%. The anti-microbial activity assay used three different types of microbes (Streptococcus pneumonia, Haemophilus influenza, and Candida albicans) different in 3 repetitions. There were significant differences each concentration on the width of clear zone among Streptococcus pneumonia (P-value = 0.010), Haemophilus influenza (P-value = 0.011), and Candida albicans (P-value = 0.005). Also, all concentrations of ginger flower extract showed no significant difference between positive controls; however, significant differences were found for negative controls. Hence, It can be concluded that 25% of ginger flower extract has been sufficient to inhibit the growth of various tested microorganisms.

scholarly journals Uji aktivitas antifungi ekstrak etanol daun cabai jawa (Piper retrofractum) terhadap pertumbuhan candida albicans

2013 ◽  
Vol 11 (2) ◽  
pp. 36-42
Author(s):  
EVI ROSYIDA SARI ◽  
ESTU RETNANINGTYAS NUGRAHENI
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Activity Test ◽  
Significant Difference ◽  
Piper Retrofractum ◽  
One Way Anova ◽  
Concentration Series ◽  
Selection Of

Sari ER, Nugraheni ER. 2013. Antifungal activity test of Piper retrofractum leaf ethanol extract on Candida albicans growth. Biofarmasi 13: 36-42. Candida albicans is a pathogenic microbe infecting vagina, thrush (fungal infection on mouth cavity) and paronichia (the presence of pus on nail pad). The less effective treatment system, and the incidence of toxicity on several antifungal lead to the selection of alternative medication (treatment) from the secondary metabolite compounds of flavonoid, alkaloid and saponin existing in java chili (Piper retrofractum Vahl) leaf. This research aimed to examine the antifungal activity of javanese chili (Piper retrofractum Vahl) leaf ethanol extract on Candida albicans, as well as to determine the antifungal properties of antifungal activity of javanese chili leaf ethanol extract. The extract was obtained by maceration using 70% ethanol. The ethanol extract obtained was 57.895 gram with the specimen of 7.61% (b/w). The result of phytochemical screening showed the presence of flavonoid, saponin and alkaloid compounds, all of those were antifungal. The examination of antifungal activity of javanese chili leaf ethanol extract was performed by using a diffusion method and the concentration series of 10-100% by adding DMSO as a diluent and it was performed with 3 times of repetition. The result of DDH was analyzed using CRD (Completely Random Design) with one-way ANOVA at a confidence interval of 95% and LSD to find out the significant difference between the concentration series. The result of antifungal activity examination showed that the ethanol extract at 40% concentration provided more effective DDH on C. albicans of 5.54±0,64 mm. The result of examination was processed by using one-way ANOVA and LSD indicating a significant difference (p<0.05) between the concentration series. At 40% concentration, the antifungal examination was performed on C. albicans. The result showed that the javanese chili leaf ethanol extract was a fungiostatic against the tested fungus.

scholarly journals Uji Aktivitas Ekstrak Etanol Daun Lidah Buaya (Aloe Vera) Terhadap Penghambatan Pertumbuhan Staphylococcus aureus Dan Candida albicans

2019 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Gemy Nastiti Handayani
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Test Results ◽  
Key Words Ethanol ◽  
Clear Zone ◽  
Anti Fungal

This study aims to determine the activity of ethanol extract of Aloe vera leaves against Staphylococcus aureus bacteria and Candida albicans fungi. Extraction of aloe vera leaves is done by maceration method, then extracted several concentrations, namely 2.5%, 3.5%, and 4.5% for Staphylococcus aureus bacteria and concentrations of 4%, 8% and 12% for Candida albicans fungi. The inhibitory testing is done by agar diffusion method. The test results showed that the ethanol extract of Aloe vera leaves had antibacterial activity of Staphylococcus aureus and anti-fungal Candida albicans which was characterized by the presence of a clear zone. In Staphylococcus aureus bacteria provide optimum inhibition zone at a concentration of 4.5% with a diameter of 10.8 mm while in, the fungus Candida albicans provides the optimum inhibition zone at a concentration of 12% with a diameter of resistance 18.87 mm. Key Words: Ethanol, Aloe vera leaves, Staphylococcus aureus, Candida albicans

scholarly journals Anti-Fungal Activity of Cathelicidins and their Potential Role in Candida albicans Skin Infection

2005 ◽  
Vol 125 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Belén López-García ◽  
Phillip H.A. Lee ◽  
Kenshi Yamasaki ◽  
Richard L. Gallo
Keyword(s):  
Candida Albicans ◽  
Potential Role ◽  
Skin Infection ◽  
Fungal Activity ◽  
Anti Fungal

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

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