scholarly journals Maskulinisasi larva ikan nila (Oreochromis niloticus) melalui penggunaan madu dengan konsentrasi berbeda

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Soesanti S Odara ◽  
Juliaan Ch Watung ◽  
Hengky J Sinjal
Keyword(s):  
Experimental Design ◽  
Red Blood Cells ◽  
Analysis Of Variance ◽  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Nile Tilapia ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to determine the effect of concentration of honey on masculinization of nile tilapia female. The study had four treatments, each with three replications. The experimental design used was completely randomized design (RAL).  Data was analyse using Analysis Of Variance (ANOVA) followed by LSD at 5% and 1%.  Broodstock was  soaked in 1 L of water with treatment A 0 mL (without honey), B (5 mL honey), C (10 mL honey), D (15 mL honey) for 10 hours. After soaking, fish was reared in pan for one month. The percentage of male was determine by comparing the length and width of the red blood cells under a microscope.  The results showed the the use of honey had significant effect on female masculinization.  The percentage of male larvae tended to increase with the increase of honey dose. The highest percentage of male was obtained in treatment D (15 ml) that was 80%.   Keywords: Honey, masculinization, larvae, Oreochromis niloticus

scholarly journals The Effect of Breadfruit Leaf Flour (Artocarpus altilis) on Number of Blood Cells And Correlation Between Cholesterol Blood and Meat of Tegal Ducks 10 Weeks Age

2018 ◽  
Vol 19 (3) ◽  
pp. 179
Author(s):  
Elly Tugiyanti ◽  
Emmy Susanti
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regression Coefficient ◽  
White Blood Cells ◽  
Blood Cholesterol ◽  
Blood Profile ◽  
Artocarpus Altilis ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design

The purpose of this research was to know the effect of breadfruit leaves flour in feed to blood profile and correlation between cholesterol, HDL and LDL of blood with meat cholesterol of maleTegal duck of 10 weeks old. The material used was Day Old Duck of male Tegal duck  as much as 120 heads with experimental research methods and Completely Randomized Design (CRD). There were 6 treatments: feed without breadfruit leaf powder, feed + breadfruit flour 3% / kg feed, feed + breadfruit flour 6% / kg feed, feed + breadfruit flour 9% / kg feed, feed + flour breadfruit 12% / kg feed, and feed + breadfruit flour 15% / kg feed. Each treatment was repeated 5 times and each replication consisted of 4 ducks. The variables observed were the number of red blood cells, white blood cells, HDL, LDL, blood triglycerides, correlation between cholesterol, HDL and LDL blood with meat cholesterol of male Tegal duck of 10 weeks old.  The result of the analysis showed that the supplementation of breadfruit leaves has significant effect (P <0,05) on the number of red blood cells, white blood cells, HDL, and blood triglyceride. There is a positive correlation between blood cholesterol with duck meat cholesterol which given breadfruit leaf powder. This research can be concluded that the supplementation of breadfruit leaf powder (Artocarpus altilis) can increase the number of red blood cells, white blood cells, blood HDL level but lowers blood cholesterol and triglyceride blood of male Tegal duck of 10 weeks old. Cholesterol meat is affected by blood cholesterol with regression coefficient of 0.780941, influenced by LDL (r = 0.407849), but negatively affected by HDL (r = -0.47022).

scholarly journals Daya Tetas Telur dan Sintasan Larva Dari Hasil Penambahan Madu pada Bahan Pengencer Sperma Ikan Nila (Oreochromis niloticus)

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Yunus Ayer ◽  
Joppy Mudeng ◽  
Hengky Sinjal
Keyword(s):  
Experimental Design ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Larval Survival ◽  
Hatching Rate ◽  
Dilution Ratio ◽  
Egg Hatching ◽  
Research Results ◽  
Spermatozoa Motility ◽  
Randomized Design

The objective of research was to determine the concentration of honey in sperm dilution  for improving hatching rate of egg and survival of nile tilapia larvae (Oreochromis niloticus).The number of fish used was five pairs broodstock. Dilution substance was NaCl and honey.  Observations were conducted on spermatozoa motility, fertility and egg hatching rate. Experimental design used was complete randomized design. Sperm dilution substance was prepare by disolving honey  (0 mL; 0,60 mL; 0,65 mL dan 0,70 mL) in 100 mL; 99,40 mL; 99,35 mL and  99,30 mL NaCl respectively.  Each dilution was homogenized using aerator for 15 minutes. Fertilization was done 12 hours after ovulation.  Egg hatching rate was observed after ovulation. Research results showed the use of honey had significant effet on egg hatching rate, but not on larval survival.  Treatment D (0,70 mL honey in 99,30 mL NaCl) had the highest hatching rate (77.33%). Dilution ratio 1:60 was the best indicated by spermatozoa motility 96.66%, fertility 71.65, hatching rate 70% and larval survival 81.67%   Keywords : honey, egg hatching rate, larval survival, Oroechromnis niloticus

scholarly journals The Effect of Gatot (Fermented Dried Cassava) and Red Bean Ratio on Water Content and Organoleptic Characteristics of The "Gatotkaca" Analog Rice

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Eries Kusmiandany ◽  
Yoga Pratama ◽  
Yoyok Budi Pramono
Keyword(s):  
Experimental Design ◽  
Water Content ◽  
Analysis Of Variance ◽  
Significance Level ◽  
Organoleptic Characteristics ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Red Bean

This study aimed to evaluate the effect of the ratio of gatot and red beans usage on water content and organoleptic characteristics from "Gatotkaca" analog rice. The material in this study was gatot (fermented cassava) and red beans. The experimental design used was a completely randomized design (CRD) with 4 treatments and 5 replications. This study consisted of 4 different analog rice formulations, namely T1 (90% gatot: 10% red beans); T2 (85% gatot: 15% red beans); T3 (80% gatot: 20% red beans); T4 (75% gatot: 25% red beans). Data were analysed using Analysis of Variance (ANOVA) with the significance level 5%. The results showed that the difference in the ratio of the usage of gatot but red beans didn’t have a significant effect (p>0.05) on the water content and organoleptic characteristics. Treatment with a ratio of gatot and 85% red beans  15% gatot was the best treatment.

scholarly journals Pengaruh Konsentrasi IgY Induk yang Berbeda Terhadap Gambaran Darah Anak Ayam Sentul

2019 ◽  
Vol 6 (2) ◽  
pp. 273
Author(s):  
Melly Pratiwi Setyawati ◽  
Niken Ulupi ◽  
Sri Murtini ◽  
Cece Sumantri
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
T Test ◽  
Blood Picture ◽  
Test Program ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference

ABSTRAKPenelitian ini bertujuan untuk menganalisis gambaran sel darah putih dan sel darah merah sebagai indikator ketahanan tubuh pada anak ayam sentul. Penelitian ini menggunakan 20 ekor ayam betina dan 20 ekor ayam jantan yang dikawinkan, masing masing terdiri dari 10 ekor ayam dengan konsentrasi IgY tinggi dan 10 ekor ayam dengan konsentrasi IgY rendah. Berdasarkan hasil perkawinan tersebut diperolah 34 ekor anak ayam yang terdiri dari 17 ekor anak ayam yang berasaldari induk dengan konsentrasi IgY rendah. Parameter yang diamati meliputi gambaran sel darah merah (eritrosit, hematocrit dan indeks eritrosit) dan gambaran sel darah putih (leukosit dan diferensiasinya). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 2 perlakuan, semua perlakuan diulang 10 kali. Data yang diperoleh dianalisis menggunakan t-test program SAS.  Hasil penelitian ini menunjukan bahwa perbedaan konsentrasi IgY pada induk tidak mempengaruhi sel darah merah dan sel darah putih. Namun terdapat kecendrungan nilai limfosit yang rendah dan H/L yang tinggi pada anak ayam yang berasal dari induk dengan konsentrasi IgY rendah. Hasil ini mengindikasi bahwa anak ayam yang berasal dari induk dengan konsentrasi IgY rendah lebih rentan terhadap cekaman panas. Kata Kunci: ayam sentul, IgY,  gambaran darah, ketahanan tubuhABSTRACTThis study was to aim the description of white blood cells and red blood cells as indicators of immunity in Sentul chicks. This study used 20 hen chickens and 20 cock-chicken mated, consisting of 10 chickens with high IgY concentration and 10 chickens with low IgY concentrations. Based on the results of mating, 34 chicks were obtained consisting of 17 chicks that were from the hen with low IgY concentration. The parameters observed included the description of red blood cells (erythrocytes, hematocrit and erythrocyte index) and the description of white blood cells (leukocytes and their differentiation). The experiment was conducted as a completely randomized design with 2 treatments, all treatments were repeated 10 times. The data obtained were analyzed using the SAS program t-test. The results of this study indicate that the difference in IgY concentration in the hen did not effect on red blood cells and white blood cells. However, there is a tendency for low lymphocyte values and high H/ L in chicks originating from hens with low IgY concentrations. These results indicate that chicks from hens with low IgY concentrations are more susceptible to heat stress.Keyword: blood picture, IgY, immunity, sentul chicken

scholarly journals Masculinization of Nile tilapia (Oreochromis niloticus) by administration of bull testes meal

2011 ◽  
Vol 10 (1) ◽  
pp. 51-58
Author(s):  
Muslim Muslim ◽  
M. Zairin Junior ◽  
Nur Bambang Priyo Utomo
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Fish Growth ◽  
Feed Additive ◽  
Male Population ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Two Factors ◽  
Group Survival ◽  
Completely Randomized Design

The synthetic steroid 17α-Methyltestosteron (MT) is commonly used as a feed additive to produce male population of Nile tilapia (Oreochromis niloticus). The use of synthetic testosterone hormone is not recommended in Indonesia. This study was conducted to evaluate the effect of natural testosterone hormone in bull testes meal (BTM) on the masculinization of Nile tilapia using validated aceto carmine squash method of gonads of the fish. Experimental design was utilized two factors experiments in completely randomized design. Fry kept in 40-L glass aquaria at a density of 40 fry/aquarium. Fry (7 dph) received the BTM  for 7 days (T1), 14 days (T2) and 21 days (T3) and doses 0% (D1), 3% (D2), 6% (D3), and 9% (D4).  When treatment was these results, indicated that significant (P≥0.05) masculinization occurred only in the group treated of BTM and no treated of BTM. In the group treated of BTM, doses and duration treatment is not significant. The percentage of male fish 83.3% (9%-7d, 9%-21d, 6%-21d: doses and duration, respectively), higher than all group. Survival rate of fry (95-99.5%) is not affected by treatment BTM (no significant P≥0.05). Fish growth was significantly affected by treatment BTM compare with no treated of BTM. The highest growth performance of fry were obtained with the 9% BTM.Key words: masculinization, nile tilapia, bull testes meal ABSTRAKSteroid sintetik 17α-Methyltestosteron (MT) umumnya digunakan sebagai aditif pakan untuk menghasilkan populasi ikan nila jantan (Oreochromis niloticus). Penggunaan hormon testosteron sintetis tidak dianjurkan di Indonesia. Penelitian ini dilakukan untuk mengevaluasi pengaruh hormon testosteron alami dalam makanan testis banteng (BTM) pada maskulinisasi ikan nila menggunakan metode divalidasi aceto carmine squash, dari gonad ikan. Desain eksperimental dimanfaatkan dua eksperimen faktor dalam desain benar-benar acak. Fry disimpan dalam 40-L akuarium kaca pada kepadatan 40 fry/akuarium. Fry (7 DPH) menerima BTM selama 7 hari (T1), 14 hari (T2) dan 21 hari (T3) dan dosis 0% (D1), 3% (D2), 6% (D3), dan 9% (D4). Ketika pengobatan hasil ini, menunjukkan signifikan (P ≥ 0,05) hanya terjadi maskulinisasi pada kelompok perlakuan dari BTM dan tidak diperlakukan BTM. Pada kelompok diobati BTM, dosis dan durasi pengobatan tidak signifikan. Persentase ikan jantan 83,3% (9%-7d, 9%-21d, 6%-21d: dosis dan durasi, masing-masing), lebih tinggi dari kelompok semua. Tingkat kelangsungan hidup benih (95-99,5%) tidak dipengaruhi oleh pengobatan BTM (tidak ada P yang signifikan ≥ 0,05). Pertumbuhan ikan secara signifikan dipengaruhi oleh BTM pengobatan dibandingkan dengan tidak diobati BTM. Kinerja pertumbuhan tertinggi fry diperoleh dengan BTM 9%.Kata kunci: maskulinisasi, ikan nila, tepung testis sapi 

scholarly journals UTILIZATION OF TOFU AND TAPIOCA INDUSTRIAL LIQUID WASTE FOR NILE TILAPIA (Oreochromis niloticus) CULTURE WITHIN DIFFERENT BIOFLOC SYSTEMS

2018 ◽  
Vol 7 (1) ◽  
pp. 763
Author(s):  
Suryo Kunindar ◽  
Eko Efendi ◽  
Supono Supono
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Liquid Waste ◽  
Daily Growth Rate ◽  
Daily Growth ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Absolute Growth ◽  
Completely Randomized Design ◽  
Culture Container

Liquid waste produced by tofu and tapioca industry was approximately 1,5-2 m3 and 4-6 m3 per day respectively. Tapioca liquid waste has concentration of carbon around 119,11 mg/l, while tofu liquid waste has around 133,03 mg/l of nitrogen in concentration. Therefore both of these waste have the potential to be used as biofloc that utilized as additional feed with high protein content for nile tilapia. The aim of this research was to know interaction between C/N ratio and place of biofloc production to the growth of nile tilapia. This research used completely randomized design based on factorial experiment which consisted of two level of each factor and three repetition. Level of C/N ratio were 15 and 20 whereas level of place of biofloc production were inside and outside fish culture container. The result of this research showed that interaction between place of biofloc production and C/N ratio affected the growth of nile tilapia. The treatment inside biofloc production with 20 C/N ratio gave provided the highest absolute growth (3,26 g) and daily growth rate (0,082 g per day).

scholarly journals Profitability of Using Commercial Tilapia Feed to Produce Three Different Sizes of Nile Tilapia (Oreochromis niloticus L.) Fingerlings in Ghana

2021 ◽  
pp. 10-17
Author(s):  
F. A. Anani ◽  
E. Agbeko ◽  
P. D. K. Atsakpo ◽  
M. Johnson-Ashun ◽  
L. K. Osei ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Fish Growth ◽  
Fine Mesh ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Body Weights ◽  
Weight Gains ◽  
Completely Randomized Design ◽  
Individual Body

Aims: To determine the profitability of using a commercial tilapia feed to produce three different size ranges (1.0-1.9, 2.0-2.9 and 5.0-5.9 g) of Nile tilapia, Oreochromis niloticus fingerlings in hapa-in-pond system. Study Design: Completely randomized design. Place and Duration of Study: The Aquaculture Research and Development Centre (ARDEC), Akosombo, of Water Research Institute (WRI) of Council for Scientific and Industrial Research (CSIR), Ghana, from March to May, 2020. Methodology: Fish growth study was carried out in three (3) fine mesh netting hapas, each of dimensions 5.0 x 2.0 x 1.2 m. Nile tilapia fry at initial mean weight 0.03 ± 0.01 g were stocked at a density of 50 fish m-2 and they were fed at 20 % body weight five times daily. The feeding of the fish continued until those in all the 3 hapas attained a mean weight of at least 5.0 g. Then the experiment was terminated and all the survived fish in each hapa were harvested, counted, and their individual body weights were measured. Growth performance indicators and profitability of producing the various size categories (1.0-1.9, 2.0-2.9 and 5.0-5.9) were determined. Results: The fry attained the target size ranges of 1.0-1.9, 2.0-2.9 and 5.0-5.9 g in 4, 6 and 9 weeks respectively. There were significant differences (ANOVA, P = 0.03) among final mean weights, weight gains, feed intakes, daily weight gains, feed efficiencies and harvested biomass among all size ranges, with those of 5.0-5.9 g being significantly higher (Tukey’s HSDT, P < 0.02). Sizes of fingerlings produced correlate positively with cost of feed used. The profit indices ranged from 2.57 to 10.22, with the highest recorded in the 1.0-1.9 g fingerlings and the least in those of 5.0-5.9 g. Conclusion: The results indicated that, at the current Nile tilapia fingerlings cost and the time taken to produce the various size categories, the 1.0-1.9 g production is the most profitable.

scholarly journals PEMBERIAN PAKAN KOMERSIL DENGAN PENAMBAHAN PROBIOTIK YANG MENGANDUNG Lactobacillus sp. TERHADAP KECERNAAN DAN PERTUMBUHAN IKAN NILA (Oreochromis niloticus) (THE ADDITION OF PROBIOTICS CONTAINING Lactobacillus Sp. IN THE COMMERCIAL ON DIGESTIBILITY AND GROWTH OF NILE TILAPIA (OREOCHROMIS NILOTICUS)

2016 ◽  
Vol 2 (4) ◽  
pp. 178
Author(s):  
Sri Ramadhana ◽  
Noor Arida Fauzana ◽  
Pahmi Ansyari
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nutritional Content ◽  
Length Growth ◽  
Environmental Friendly ◽  
Randomized Design ◽  
Commercial Feed ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Effective Doses ◽  
Completely Randomized Design

Tujuan dari penelitian ini adalah untuk mengetahui efek pemberian probiotik yang mengandung Lactobacillus sp. dalam pakan komersial terhadap kualitas pakan, meningkatkan jumlah bakteri dalam mukosa usus dan kecernaan ikan melalui pemberian pakan probiotik yang ramah lingkungan, mengetahui dosis efektif probiotik dalam pakan yang dapat menunjang pertumbuhan ikan nila (Oreocromis niloticus). Penelitian ini dilaksanakan selama 6 (enam) bulan, mulai bulan September 2012 sampai Februari 2013. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap dengan 4 (Empat) perlakuan dan 3 (Tiga) ulangan, yaitu perlakuan A (Kontrol), B (Probiotik 3%), C (Probiotik 5%) dan D (Probiotik 7%).Hasil penelitian menunjukkan bahwa pemberian pakan dengan penambahan probiotik yang mengandung Lactobacillus sp. menggunakan dosis sebesar 3%, 5% dan 7% mampu meningkatkan kandungan gizi, pakan, dengan nilai protein antara 32,48%-34,19% dan menurunkan serat kasar dengan nilai antara 5,04%-5,70% dibandingkan tanpa pemberian probiotik. Pemberian probiotik dalam pakan dengan persentase 3%, 5%, dan 7% dapat meningkatkan kecernaan ikan nila pakan efektif untuk meningkatkan pertumbuhan berat dan panjang ikan nila.The purpose of this research was to determine the effect of probiotics in commercial feed on feed quality, enhance increase bactery number in gut mucose and digestibility of nile tilapia (Oreocromis niloticus) through the provision of environmental friendly probiotic feed, determine  the effective doses of probiotics infeed that can support the growth fish. The research was conducted for 6 (six) months, from September 2012 until February 2013. The design used in this study was completely randomized design with 4 (four) treatments and 3 (three) replicates, were treatment A (control), B (Probiotics 3%), C (Probiotics 5%) and D (Probiotic 7%).The results showed that the addition of probiotic containing Lactobacillus sp. feeding with doses of 3%, 5% and 7% were able to improve the nutritional content, protein values between 32,48%-34,19% and lower crude fiber with values between 5,04-5,70% compared with no probiotics. The addition of probiotics in feed with a percentage of 3%, 5%, and 7% can increase digestibility of nile tilapia and effective to increase the nile weight and length growth of nile tilapia.

scholarly journals Pesisir cattle superovulation with various dosage of Follicle Stimulating Hormone (FSH) on embrio production

2021 ◽  
Vol 888 (1) ◽  
pp. 012034
Author(s):  
T Afriani ◽  
E Purwati ◽  
J Hellyward ◽  
Jaswandi ◽  
M Mundana ◽  
...  
Keyword(s):  
Experimental Design ◽  
Corpus Luteum ◽  
Analysis Of Variance ◽  
Embryo Quality ◽  
Follicle Stimulating Hormone ◽  
Research Material ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Stimulating Hormone

Abstract This study aimed to determine the use of various doses of FSH hormone, the number of lutheum corpus and the number and quality of embryos produced in the superovulation program in Pesisir cattle. The experimental design was Analysis of Variance (Anova) in a completely randomized design. The research material used 16 cows from Pesisir cattle that had been synchronized with estrous with CIDR implants for 13 days and were injected with the PGF2α hormone after the release of CIDR, then scheduled AI was performed. The dose of FSH hormone was 16 ml, 17 ml, 18 ml and 19 ml, each treatment consisting of 4 replications. The injection of the FSH hormone was carried out for 3 days at a decreased dose. Collection of donor embryos was carried out on days 6 to 8 after the scheduled AI. The parameters observed included superovulation response, number of corpus luteum, number of embryos and quality of embryos. The results showed that the response of Pesisir cattle superovulation was 81.25%. The average number of CL obtained was 13.75 and embryos for Pesisir cattle was 11.25. The results also showed that the quality of the embryos obtained were grade A 19, grade B 18, grade C 7, grade D 1. It can be concluded that the use of various doses of FSH hormone at doses of 16 mg, 17 ml, 18 ml and 19 ml had shown a superovulatory response. The 17 ml dose of FSH hormone showed a large number of embryos and had the best embryo quality.

scholarly journals Effectiveness of Sansavieria trifasciata Extract Bracelet in Absorbing Carbon Monoxide (Co) in Cigarette Smoke

Jurnal Biota ◽  
1970 ◽  
Vol 5 (2) ◽  
pp. 96-103
Author(s):  
Gendis Siti Rahmawati ◽  
Resti Nuraeni ◽  
Lu’lu Robiatul Fajrin ◽  
Sistiana Windyariani
Keyword(s):  
Carbon Monoxide ◽  
Red Blood Cells ◽  
Experimental Method ◽  
Blood Vessels ◽  
Cigarette Smoke ◽  
Blood Cells ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Randomized Design ◽  
Completely Randomized Design

Carbon monoxide (CO) contained in cigarette smoke is a very strong poison, if it is smoked by the lungs it will be easier to bind hemoglobin, so that red blood cells will lack oxygen, which can cause damage to blood vessels. with the process of atherosclerosis (narrowing). Sansevieria trifasciata has the ability to absorb poisons such as CO. This study aims to determine the effectiveness of Sansevieria trifasciata leaf extract in absorbing CO contained in cigarette smoke and assessing the amount of CO absorbed by Sansevieria trifasciata leaf extract. The method used is a pure experimental method using a Completely Randomized Design (CRD) consisting of 3 different treatments and 3 repetitions. The results of the 3 treatments showed that P1 (Sanseviera trifasciata wet extract) had the ability to absorb CO an average of 12.67 ppm every 15 minutes, in contrast to P2 (ethanol extract of Sanseviera trifasciata) which absorbs CO. 91.11 ppm every 15 minutes P3 (ethanol extract and CMC leaves from Sansevieria trifasciata) has the ability to absorb CO by 37.89 ppm every 15 minutes. The highest absorption effectiveness was obtained by ethanol extract and bracelets containing ethanol extract with the addition of CMC.

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