scholarly journals Pengaruh Konsentrasi IgY Induk yang Berbeda Terhadap Gambaran Darah Anak Ayam Sentul

2019 ◽  
Vol 6 (2) ◽  
pp. 273
Author(s):  
Melly Pratiwi Setyawati ◽  
Niken Ulupi ◽  
Sri Murtini ◽  
Cece Sumantri
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
T Test ◽  
Blood Picture ◽  
Test Program ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference

ABSTRAKPenelitian ini bertujuan untuk menganalisis gambaran sel darah putih dan sel darah merah sebagai indikator ketahanan tubuh pada anak ayam sentul. Penelitian ini menggunakan 20 ekor ayam betina dan 20 ekor ayam jantan yang dikawinkan, masing masing terdiri dari 10 ekor ayam dengan konsentrasi IgY tinggi dan 10 ekor ayam dengan konsentrasi IgY rendah. Berdasarkan hasil perkawinan tersebut diperolah 34 ekor anak ayam yang terdiri dari 17 ekor anak ayam yang berasaldari induk dengan konsentrasi IgY rendah. Parameter yang diamati meliputi gambaran sel darah merah (eritrosit, hematocrit dan indeks eritrosit) dan gambaran sel darah putih (leukosit dan diferensiasinya). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 2 perlakuan, semua perlakuan diulang 10 kali. Data yang diperoleh dianalisis menggunakan t-test program SAS.  Hasil penelitian ini menunjukan bahwa perbedaan konsentrasi IgY pada induk tidak mempengaruhi sel darah merah dan sel darah putih. Namun terdapat kecendrungan nilai limfosit yang rendah dan H/L yang tinggi pada anak ayam yang berasal dari induk dengan konsentrasi IgY rendah. Hasil ini mengindikasi bahwa anak ayam yang berasal dari induk dengan konsentrasi IgY rendah lebih rentan terhadap cekaman panas. Kata Kunci: ayam sentul, IgY,  gambaran darah, ketahanan tubuhABSTRACTThis study was to aim the description of white blood cells and red blood cells as indicators of immunity in Sentul chicks. This study used 20 hen chickens and 20 cock-chicken mated, consisting of 10 chickens with high IgY concentration and 10 chickens with low IgY concentrations. Based on the results of mating, 34 chicks were obtained consisting of 17 chicks that were from the hen with low IgY concentration. The parameters observed included the description of red blood cells (erythrocytes, hematocrit and erythrocyte index) and the description of white blood cells (leukocytes and their differentiation). The experiment was conducted as a completely randomized design with 2 treatments, all treatments were repeated 10 times. The data obtained were analyzed using the SAS program t-test. The results of this study indicate that the difference in IgY concentration in the hen did not effect on red blood cells and white blood cells. However, there is a tendency for low lymphocyte values and high H/ L in chicks originating from hens with low IgY concentrations. These results indicate that chicks from hens with low IgY concentrations are more susceptible to heat stress.Keyword: blood picture, IgY, immunity, sentul chicken

scholarly journals The Effect of Breadfruit Leaf Flour (Artocarpus altilis) on Number of Blood Cells And Correlation Between Cholesterol Blood and Meat of Tegal Ducks 10 Weeks Age

2018 ◽  
Vol 19 (3) ◽  
pp. 179
Author(s):  
Elly Tugiyanti ◽  
Emmy Susanti
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regression Coefficient ◽  
White Blood Cells ◽  
Blood Cholesterol ◽  
Blood Profile ◽  
Artocarpus Altilis ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design

The purpose of this research was to know the effect of breadfruit leaves flour in feed to blood profile and correlation between cholesterol, HDL and LDL of blood with meat cholesterol of maleTegal duck of 10 weeks old. The material used was Day Old Duck of male Tegal duck  as much as 120 heads with experimental research methods and Completely Randomized Design (CRD). There were 6 treatments: feed without breadfruit leaf powder, feed + breadfruit flour 3% / kg feed, feed + breadfruit flour 6% / kg feed, feed + breadfruit flour 9% / kg feed, feed + flour breadfruit 12% / kg feed, and feed + breadfruit flour 15% / kg feed. Each treatment was repeated 5 times and each replication consisted of 4 ducks. The variables observed were the number of red blood cells, white blood cells, HDL, LDL, blood triglycerides, correlation between cholesterol, HDL and LDL blood with meat cholesterol of male Tegal duck of 10 weeks old.  The result of the analysis showed that the supplementation of breadfruit leaves has significant effect (P <0,05) on the number of red blood cells, white blood cells, HDL, and blood triglyceride. There is a positive correlation between blood cholesterol with duck meat cholesterol which given breadfruit leaf powder. This research can be concluded that the supplementation of breadfruit leaf powder (Artocarpus altilis) can increase the number of red blood cells, white blood cells, blood HDL level but lowers blood cholesterol and triglyceride blood of male Tegal duck of 10 weeks old. Cholesterol meat is affected by blood cholesterol with regression coefficient of 0.780941, influenced by LDL (r = 0.407849), but negatively affected by HDL (r = -0.47022).

scholarly journals Haematological and Genotoxic effects of cadmium chloride on Mesopotamichthys sharpeyi

2016 ◽  
Vol 13 (4) ◽  
pp. 745-752
Author(s):  
Baghdad Science Journal
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Cadmium Chloride ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Blood Picture ◽  
Genotoxic Effects

The present study investigated Haematological changes in Mesopotamichthys sharpeyi, as well as determination genotoxic effects of cadmium chloride on bunni fish by using 120 fingerlings, fish were distributed randomly into four treatments in addition to control group. Fish in first group treated (T1) with cadmium 0.093mg/L with changing water and added cadmium continuously, fish in the second group treated (T2) with cadmium 0.093mg/L with changing water without adding cadmium, third treatment (T3) with cadmium 0.046mg/L with changing water and adding cadmium continuously, and fourth treatment (T4) with cadmium 0.046mg/L with changing water without adding cadmium. Results of blood picture in T1 and T3 showed a significant reduction in red blood cells count, hemoglobin concentration, packed cell volume values, while the number of white blood cells showed a significant increase in its values. Results showed presence of improvement of clinical and microscopical signs and blood picture in T2 and T4, were changed water aquarium continuously and added cadmium only once compared withT1 and T3. Results of the present study concluded that changing water aquarium in the treatments without adding cadmium led to improvement of health status of fish which increased with the passage of time results of blood picture were almost the same of the control group. It could be concluded from the current study that the adding of cadmium to water aquarium containing bunni fish led to decrease in red blood cells count, hemoglobin and packed cell volume values and increase in micronuclei number.

scholarly journals Effectiveness of Sansavieria trifasciata Extract Bracelet in Absorbing Carbon Monoxide (Co) in Cigarette Smoke

Jurnal Biota ◽  
1970 ◽  
Vol 5 (2) ◽  
pp. 96-103
Author(s):  
Gendis Siti Rahmawati ◽  
Resti Nuraeni ◽  
Lu’lu Robiatul Fajrin ◽  
Sistiana Windyariani
Keyword(s):  
Carbon Monoxide ◽  
Red Blood Cells ◽  
Experimental Method ◽  
Blood Vessels ◽  
Cigarette Smoke ◽  
Blood Cells ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Randomized Design ◽  
Completely Randomized Design

Carbon monoxide (CO) contained in cigarette smoke is a very strong poison, if it is smoked by the lungs it will be easier to bind hemoglobin, so that red blood cells will lack oxygen, which can cause damage to blood vessels. with the process of atherosclerosis (narrowing). Sansevieria trifasciata has the ability to absorb poisons such as CO. This study aims to determine the effectiveness of Sansevieria trifasciata leaf extract in absorbing CO contained in cigarette smoke and assessing the amount of CO absorbed by Sansevieria trifasciata leaf extract. The method used is a pure experimental method using a Completely Randomized Design (CRD) consisting of 3 different treatments and 3 repetitions. The results of the 3 treatments showed that P1 (Sanseviera trifasciata wet extract) had the ability to absorb CO an average of 12.67 ppm every 15 minutes, in contrast to P2 (ethanol extract of Sanseviera trifasciata) which absorbs CO. 91.11 ppm every 15 minutes P3 (ethanol extract and CMC leaves from Sansevieria trifasciata) has the ability to absorb CO by 37.89 ppm every 15 minutes. The highest absorption effectiveness was obtained by ethanol extract and bracelets containing ethanol extract with the addition of CMC.

scholarly journals Haematological and biochemical indices of broiler chicken fed graded levels of boiled African yam beans (Sphenostylis stenocarpa)

2020 ◽  
Vol 44 (2) ◽  
pp. 139-144
Author(s):  
A. S. Eburuaja ◽  
D. N. Onunkwo ◽  
O. O. Adedokun
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Cells ◽  
Broiler Chicken ◽  
White Blood Cells ◽  
Treatment Groups ◽  
Randomized Design ◽  
Optimal Productivity ◽  
Mean Corpuscular Haemoglobin ◽  
Completely Randomized Design ◽  
Biochemical Indices

This study was carried out to investigate the haematological and biochemical indices of broiler chicken fed graded levels of boiled African yam beans. The birds, 120 in number were allotted into four treatment groups having three replicate per treatment and 10 birds per replicate in a completely randomized design were used. Diet 1 was the control and corn-soy bean-based while diets 2, 3, and 4 had boiled African am beans at 10, 15 and 20% respectively. Blood samples were collected from eight weeks old broiler chicken into two sets of labeled sterile bottles and used for the analysis. The values observed for white blood cells (2.43, 2.71, 2.72. 2.76), packed cell volume (31.00, 31.04, 28.00, 27.00) and mean corpuscular haemoglobin (32.83, 32.70, 29.60, 33.93%) for diets 1, 2, 3 and 4 respectively fell within the normal range reported for healthy broiler chicken while the red blood cells for diets 1, 2, and 3 are comparable. Blood of birds in Diets 2, 3 and 4 had similar values of alkaline phosphatase which were higher than those of diet 1. Those in diets3 and 4 had highest values of urea (30.00mg/dl), creatinine (0.413mg/dl) and lowest values of albumen (1.500d/L). Feeding boiled African yam bean above 10% level of inclusion resulted in increase in alkaline phosphatase, urea and creatinine. For optimal productivity 10% inclusion of boiled African yam beans is therefore recommended.

scholarly journals Haematological and biochemical profile of Friesian x Bunaji heifers supplemented with inorganic selenium and vitamin E

2020 ◽  
Vol 46 (2) ◽  
pp. 80-86
Author(s):  
N. P. Achi ◽  
P. P. Barje ◽  
M. Abdulrashid ◽  
O. M. Daudu
Keyword(s):  
Vitamin E ◽  
Blood Cells ◽  
Biochemical Analysis ◽  
White Blood Cells ◽  
Biochemical Profile ◽  
Randomized Design ◽  
Inorganic Selenium ◽  
Healthy Cattle ◽  
Completely Randomized Design ◽  
Apparently Healthy

A study was conducted using twenty Friesian x Bunaji heifers to evaluate the effect of supplementing inorganic feed grade Selenium and Vitamin E on the haematological and biochemical profile. The study consists of four treatments with five animals per Treatment in a Completely Randomized Design (CRD). Treatment 1 served as Control without Selenium or Vitamin E supplementation. Treatment 2 was supplemented with 3 mg of feed gradeSelenium. Treatment 3 with 20 mg of feed grade Vitamin E. Treatment 4 had a combination of 3 mg feed grade Selenium and 20 mg of Vitamin E. Two sets of blood samples were taken from the animal via jugular venipuncture using a 10 mL syringe fitted with a needle into sample bottles for haematological and biochemical analysis. Neutrophils (19.78 - 29.88%) were similar (p<0.05) and Se supplementation recording the highest value. Packed cell volume(19.14 – 22.22%), Haemoglobin, Red blood cells, White blood cells, Eosinophil, Monocytes, Basophil and Lymphocytes analyzed were similar (p>0.05). It was observed that there were similarities (p>0.05) in Glucose, Serum protein, globulin, urea N, albumin, MDA protein, AST, ALT and ALP analyzed except for GSH-Px (22.38 – 24.70 μ/ml). Glutathione peroxidase was higher (p<0.05) in Friesian x Bunaji heifers supplemented with vitamin E and lowest with no supplementation. All parameters analyzed were within the normal range for apparently healthy cattle thereby suggesting that Selenium and Vitamin E as antioxidant are effective in scavenging for free radicals and reducing oxidative damage. 

scholarly journals PENGARUH ASAP ANTI NYAMUK KERTAS BAKAR TERHADAP JUMLAHERITROSIT PADA MENCIT (Mus musculus L.)

2018 ◽  
Vol 2 (1) ◽  
pp. 40
Author(s):  
Choirun Niswah ◽  
Syarifah Syarifah ◽  
Fitria Sany
Keyword(s):  
Red Blood Cells ◽  
Mus Musculus ◽  
Blood Cells ◽  
Research Method ◽  
Insect Repellent ◽  
Mosquito Repellent ◽  
Tropical Country ◽  
Randomized Design ◽  
Teaching Faculty ◽  
Completely Randomized Design

Indonesia is a tropical country encountered species of mosquitoes, such as Aedes aegypti and Culex quinquefasciatus. One of the efforts made to eradicate mosquitoes is to use mosquito repellent. Mosquito repellent is one type of insect killers household pesticide (insecticide) containing dangerous transfultrin. This study aims to determine the effect of anti-mosquito smoke burn paper against the number of erythrocytes in mice (Mus musculus L.). The study was conducted in September 2016 in the Laboratory of Mathematics of Tarbiyah and Teaching Faculty of UIN Raden Fatah Palembang. The research method using a completely randomized design (CRD) with treatment P0 (without exposure to insect repellent), P1 (anti mosquito burn paper, 2 hours / day), P2 (anti mosquito burn paper, 4 hours / day), P3 (anti mosquito burn paper, 6 hours / day) each with 6 replications, for 20 days. On the last day to do the stabbing in the tail to draw blood, were calculated against the number of red blood cells (erythrocytes) by using Haemocytometer. The results showed that exposure to fuel anti-mosquito burn paper affect the red blood cells of mice that P1 (anti mosquito burn paper, 2 hours / day) with an average of 8,72 x 106 / mL blood, P2 (anti mosquito burn paper, 4 hours / day) with an average of 8,87 x 106 / mL blood, P3 (anti mosquito burn paper, 6 hours / day) with an average of 7,89 x 106 / mL of blood. Effect of anti-mosquito smoke burn the most significant papers in a decrease in the number of erythrocytes of mice (Mus musculus L.) is 6 hours of exposure to an average of 7,89 x 106 / mL. The result of Anova test showed F count > Ftable namely 7,80 > 4,40 it means the treatment has significance.

scholarly journals Maskulinisasi larva ikan nila (Oreochromis niloticus) melalui penggunaan madu dengan konsentrasi berbeda

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Soesanti S Odara ◽  
Juliaan Ch Watung ◽  
Hengky J Sinjal
Keyword(s):  
Experimental Design ◽  
Red Blood Cells ◽  
Analysis Of Variance ◽  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Nile Tilapia ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to determine the effect of concentration of honey on masculinization of nile tilapia female. The study had four treatments, each with three replications. The experimental design used was completely randomized design (RAL).  Data was analyse using Analysis Of Variance (ANOVA) followed by LSD at 5% and 1%.  Broodstock was  soaked in 1 L of water with treatment A 0 mL (without honey), B (5 mL honey), C (10 mL honey), D (15 mL honey) for 10 hours. After soaking, fish was reared in pan for one month. The percentage of male was determine by comparing the length and width of the red blood cells under a microscope.  The results showed the the use of honey had significant effect on female masculinization.  The percentage of male larvae tended to increase with the increase of honey dose. The highest percentage of male was obtained in treatment D (15 ml) that was 80%.   Keywords: Honey, masculinization, larvae, Oreochromis niloticus

The Effect of Red Blood Cells on the ADP-induced Aggregation of Human Platelets in Flow Through Tubes

1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith
Keyword(s):  
Platelet Aggregation ◽  
Shear Rate ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Mean Transit Time ◽  
White Blood Cells ◽  
Aggregate Size ◽  
Human Platelets ◽  
Induced Aggregation

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.

Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran
Keyword(s):  
Survival Rate ◽  
Experimental Design ◽  
Aromatase Inhibitor ◽  
Fish Larvae ◽  
Clarias Gariepinus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

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