scholarly journals Pengaruh Efektivitas Antibakteri Ekstrak Daun Kumis Kucing Terhadap Bakteri Streptococcus Mutans

2021 ◽  
Vol 10 (1) ◽  
pp. 189-195
Author(s):  
Rizky Mulyanti ◽  
Member Reni Putra ◽  
Dian Soraya Tanjung ◽  
Shania Salsabila
Keyword(s):  
Streptococcus Mutans ◽  
Control Group ◽  
Orthosiphon Stamineus

Pendahuluan; streptococcus mutans adalah bakteri patogen dan penyebab utama karies gigi. Maka diperlukan suatu tindakan pencegahan karies gigi dengan bahan antibakteri alami dengan kumis kucing. Tujuan; mengetahui pengaruh efektivitas antibakteri ekstrak daun kumis kucing terhadap bakteri streptococcus mutans. Metode; penelitian menggunakan eksperimental laboratoris dengan desain posttest only control group in vitro. Pengumpulan data dilakukan dengan mengukur diameter zona hambat menggunakan shear caliper. Kemudian, data dianalisis dengan uji statistik oneway ANOVA dan posthoc LSD. Hasil; bahwa diameter rata-rata zona hambat antibakteri ekstrak daun kumis kucing (Orthosiphon stamineus) dengan konsentrasi 50%, 25%, 12,5%, 6,25%, 3,125% terhadap Streptococcus mutans. bakteri adalah 11,50 ± 0,38 mm, 10,78 ± 0,22 mm, 9,90 ± 0,13 mm, 9,10 ± 0,08 mm dan 8,25 ± 0,25 mm, sedangkan kontrol negatif pada penelitian ini yaitu DMSO tidak memiliki diameter hambat (0 mm). Kesimpulan; bahwa terdapat efektivitas antibakteri ekstrak daun kumis kucing terhadap bakteri Streptococcus mutans.

scholarly journals Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

2020 ◽  
Vol 13 (06) ◽  
pp. 2050022
Author(s):  
Xiaoyue Liang ◽  
Zhaohui Zou ◽  
Zheng Zou ◽  
Changyi Li ◽  
Xiaoxi Dong ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Lactic Acid ◽  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Saline Control ◽  
Control Group ◽  
Plaque Biofilm

The main objective of this study is to evaluate the antibacterial effect of antibacterial photodynamic therapy (aPDT) on Streptococcus mutans (S. mutans) biofilm model in vitro. The selection of photosensitizers is the key step for the efficacy of photodynamic therapy (PDT). However, no studies have been conducted in the oral field to compare the functional characteristics and application effects of PDT mediated by various photosensitizers. In this research, the antibacterial effect of Methylene blue (MB)/650[Formula: see text]nm laser and Hematoporphyrin monomethyl ether (HMME)/532[Formula: see text]nm laser on S. mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT. The yield of lactic acid was analyzed by Colony forming unit (CFU) and spectrophotometry, and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy (CLSM) to evaluate the bactericidal effect on each group. Based on the results of CFU, the bacterial colonies formed by 30.4[Formula: see text]J/cm2 532[Formula: see text]nm MB-aPDT group and 30.4[Formula: see text]J/cm2 532[Formula: see text]nm HMME-aPDT group were significantly less than those in other groups, and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group. Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group. The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4[Formula: see text]J/cm2 aPDT. Therefore, aPDT suitable for energy measurement can kill S. mutans plaque biofilm, and MB-aPDT is better than HMME-aPDT.

scholarly journals UJI EFEK ANTI BAKTERI EKSTRAK BUNGA CENGKEH TERHADAP BAKTERI Streptococcus mutans SECARA IN VITRO

e-GIGI ◽  
2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Juvensius R. Andries ◽  
Paulina N. Gunawan ◽  
Aurelia Supit
Keyword(s):  
Streptococcus Mutans ◽  
Control Group ◽  
Antibacterial Effects ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Clove Extract ◽  
Post Hoc ◽  
One Way Anova

Abstrak: Minyak cengkeh berguna sebagai antibakteri alami. Minyak esensial dari cengkeh mempunyai fungsi anestetik dan antimikrobial. Zat yang terkandung dalam cengkeh yang bernama eugenol dapat membunuh bakteri termasuk bakteri yang resisten terhadap antibiotika, salah satunya adalah bakteri Streptococcus mutans. Bakteri ini merupakan mikroorganisme penyebab utama terjadinya karies. Tujuan dari penelitian ini yaitu untuk mengetahui efek antibakteri ekstrak cengkeh terhadap bakteri Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental menggunakan post test only control group design. Penelitian ini menggunakan bahan coba ekstrak cengkeh dengan konsentrasi 40%, 60%, dan 80%, Ciprofloxacin, aquades dengan pengulangan sebanyak lima kali. Data dikumpulkan dan dianalisis dengan one-way ANOVA dan post-hoc uji LSD ( = 0,05). Berdasarkan hasil uji statistik penelitian uji efek antibakteri ekstrak cengkeh terhadap bakteri streptococcus mutans secara in vitro, dapat disimpulkaan bahwa ekstrak cengkeh memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans secara in vitro. Hasil uji lanjut post-hoc uji LSD menunjukan daya hambat ekstrak cengkeh 40%, 60%, 80%, lebih kecil (p<0,05) dalam menghambat Streptococcus mutans secara in vitro dibandingkan Ciprofloxacin. Kata Kunci: Ekstrak cengkeh, Streptococcus mutans.   Abstract: Clove oil is useful as a natural antibacterial agent, essential oil of clove has anesthetic and antimicrobial effect. Substances contained in clove called eugenol can kill bacteria including antibiotic resistant bacteria, one of which is the bacteria Streptococcus mutans. This bacteria is a major cause for caries. The purpose of this study was to mengetahui clove extrack antibacterial effects againts Streptococcus mutans bacteria in vitro. This study is an experimental study using a post test only control group design. This research try using clove extract with a concentration of 40%, 60%, and 80%, Ciprofloxacin, aquades repetition five times. Data collected and analyzed by one-way ANOVA and post-hoc LSD test (α = 0.05). Based on the results of the statistical test to test the effects of anti-bacterial research clove extracts against Streptococcus mutans bacteria in vitro, can disimpulkaan that clove extracts have antibacterial effects in inhibiting the growth of Streptococcus mutans bacteria in vitro.further test result post-hoc LSD test shoved its inhibitory clove extract 40%, 60%, 80% smaller (p<0,05)in hibiting Streptococcus mutans in vitro compared Ciprofloxacin. Keywords: clove extract, Streptococcus mutans

scholarly journals DAYA HAMBAT LARUTAN BAKING SODA KONSENTRASI 70% TERHADAP BAKTERI STREPTOCOCCUS MUTANS (IN VITRO)

2020 ◽  
Vol 11 (1) ◽  
pp. 21-27
Author(s):  
Sri Hidayati ◽  
Jaja Jaja ◽  
Ida Chairanna
Keyword(s):  
Streptococcus Mutans ◽  
Research Center ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Paper Disk

Baking soda memiliki sifat alkali yang dapat menetralkan pH rongga mulut sehingga dapat menghambat proses metabolisme bakteri yang menghasilkan asam. Selain itu memiliki kemampuan mempengaruhi tekanan osmotik. Sifat hipertonik dari baking soda menyebabkan  hipotonik sel bakteri kehilangan air, sehingga sel akan dehidrasi dan akhirnya dapat membunuh sel bakteri. Penelitian ini bertujuan untuk mengetahui daya hambat larutan baking soda konsentrasi  70%  terhadap bakteri Streptococcus mutans (In vitro, merupakan penelitian eksperimental murni kelompok perlakuan dan control group design. Pembuatan larutan baking soda 70%  dan bakteri Streptococcus mutans diperoleh di Research Center Fakultas Kedokteran Gigi Universitas Airlangga. Paper disk yang berisi baking soda 70% dimasukkan oven sampai kering, control(+) Chlorhexedine 0,2% dan control(-) Aquades diinkubasi 37˚C selama 24 jam, zona yang terbentuk disekeliling paper disk diukur diameternya dalam mm. Data dikumpulkan dilakukan uji normalitas dan di cari rata-rata  serta SD, dianalisa menggunakan uji T independen. Hasil penelitian terlihat rata-rata diameter zona bening menunjukkan daya hambatnya masih belum melebihi kelompok kontrol positif dimana rata-rata kelompok perlakuan 14,21mm sedang kelompok kontrol angka rata-rata 19,42mm. Berdasarkan hasil penelitian ini disimpulkan ada perbedaan daya hambat yang terbentuk pada lempeng agar perbenihan streptococcus mutans.  Diharapkan bisa dimanfaatkan sebagai bahan antibakteri untuk mencegah karies gigi dengan pemakaian baking soda yang murah,mudah didapatkan oleh masyarakat, baik berupa kumur-kumur dan diolesan ke permukaan gigi, maupun sebagai bahan tambahan dalam pasta gigi.  Kata kunci       : Bakingsoda 70%, streptococcus mutans

scholarly journals Effectivity of Jatropha multifida L. Leaves Extract as Antibacterial on Streptococcus mutans using In Vitro Testing Methods

2021 ◽  
Vol 7 (2) ◽  
pp. 415-421
Author(s):  
Listyaning Kartiko Kinasih ◽  
Idamawati Nababan ◽  
Suci Erawati ◽  
Rouli Natasia M Simanjuntak
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Control Group ◽  
Entire Body ◽  
Body Parts ◽  
Control Group Design ◽  
The Individual ◽  
Jatropha Multifida

Intoduction: Health is the condition of the entire body and body parts of an individual who is free from disease both physically, mentally and socially, thus enabling the individual to be able to carry out daily activities more productively. However, many people neglect their own health, especially in the field of dental and oral health. Caries is one of the most common oral and dental problems. The prevalence of dental caries in Indonesia is high, namely 88.8%. Dental caries is caused by Streptococcus mutans bacteria which plays a role in the conversion of sucrose to lactic acid. Infection due to Streptococcus mutans bacteria can be treated by taking antibiotics. Apart from drugs, antibiotics can also be obtained from natural ingredients, one of which is the tintir castor plant (Jatropha multifida Linn) or commonly known as the betadine. Objective: To see the effect of the antibacterial content of tintir leaves against Streptococcus mutans bacteria and to determine the differences in the antibacterial effect of betadine leaf extract (Jatropha multifida L.) from a concentration of 25%, 50%, 75%, and 100% against Streptococcus mutans. Methods: The type of research used in this study was a laboratory experimental study with a post-test-only control group design as the research design and to test the sensitivity of bacteria using the paper disc diffusion test. Data analysis using One-Way ANOVA. Result: The results of this study indicate the effect of tintir castor leaf extract (Jatropha multifida L.) in inhibiting the growth of Streptococcus mutans bacteria.

scholarly journals Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: an in vitro study

2021 ◽  
Author(s):  
Ye Han
Keyword(s):  
Treatment Group ◽  
Cigarette Smoking ◽  
Streptococcus Mutans ◽  
Laser Scanning ◽  
Water Soluble ◽  
Laser Scanning Microscopy ◽  
Extracellular Polysaccharides ◽  
Confocal Laser ◽  
Control Group

Abstract This study aimed to investigate the differences in growth and virulence (EPSs and acidogenicity) of Streptococcus mutans biofilms (S. mutans) according to the different times of cigarette smoking (CS) treatment. S. mutans biofilms (74-hour-old) were formed on saliva-coated hydroxyapatite disks. The biofilms were treated with CS at different times per day (one time, three times, and six times/day). The control group did not receive CS treatment. Acidogenicity, dry weight, colony-forming units, water-soluble/insoluble extracellular polysaccharides, and intracellular polysaccharides were analyzed and confocal laser scanning microscopy images were obtained of the 74-h-old biofilms. The 74-h-old biofilms on sHA discs in the 6 times/day CS treatment group showed the lowest biofilm accumulation and extracellular polysaccharide amount compared with the control group and other CS treatment groups. In the CLSM study, the biofilms in the six times/day CS treatment group also showed the lowest bacterial count (live and dead cells) and EPS biovolume. CS has an obvious inhibition on the growth of S. mutans biofilms, the degree of inhibition is proportional to the number of CS treatments.

scholarly journals An In Vitro Microbial-Caries Model Used to Study the Efficacy of Antibodies to Streptococcus mutans Surface Proteins in Preventing Dental Caries

2000 ◽  
Vol 7 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Margherita Fontana ◽  
Tiffany L. Buller ◽  
Ann J. Dunipace ◽  
George K. Stookey ◽  
Richard L. Gregory
Keyword(s):  
Cell Surface ◽  
Streptococcus Mutans ◽  
Positive Control ◽  
Surface Proteins ◽  
Laser Scanning Microscopy ◽  
Control Group ◽  
Lesion Area ◽  
Lesion Depth ◽  
Caries Model

ABSTRACT The first step for a pathogenic bacterium to initiate infection is via attachment (i.e., through surface determinants) to a suitable receptor. An in vitro microbial artificial-mouth model was used to test the efficacy of polyclonal antibodies to Streptococcus mutans cell surface proteins (CsAb) and a cell surface 59-kDa protein (59Ab) in preventing S. mutans colonization and carious lesion formation. In study 1, groups of 12 human teeth specimens were inoculated with S. mutans, which were incubated with different concentrations of CsAb (A1 [positive control], sterile saline, no antibody; A2, 0.007 mg of antibody protein/ml; and A3, 0.7 mg of antibody protein/ml) for 1 h at 37°C. The negative control group (B1) was not infected and was incubated with Trypticase soy broth (TSB) without dextrose supplemented with 5% sucrose (TSBS). In study 2, the same study design was used except that 59Ab was used instead of CsAb, normal rabbit serum was used in the positive control group (A1), and TSB supplemented with 1% glucose was used as the nutrient to control for sucrose-dependent colonization. All groups were exposed for 4 days to circulating cycles of TSBS and TSB (study 1 and study 2, respectively; 30 min each, three times per day) and a mineral washing solution (21 h per day). Prior to each nutrient cycle, 1 ml of the appropriate CsAb or 59Ab solution was administered to each group and allowed to mix for 30 min before cycling was resumed. Data obtained by confocal laser scanning microscopy demonstrated the presence of a significantly smaller (P < 0.05) lesion area and a smaller total lesion fluorescence in group A3 than in group A1 for both studies. In study 1, group A2 had significantly smaller values than A1 for lesion depth and area. There were no significant differences between groups A2 and A3 for lesion area or between groups A1 and A2 for total lesion fluorescence. In study 2, there were no significant differences among groups A1 and A2 for lesion depth or between groups A2 and A3 for all of the parameters studied. In both studies, there were no significant differences between S. mutans plaque CFU numbers among any of the groups. These studies demonstrated the efficacy of CsAb and 59Ab in reducing primary caries development in this model, although the underlying mechanism remains unclear.

In vitro Antimicrobial Activities of 1-Methoxyficifolinol, Licorisoflavan A, and 6,8-Diprenylgenistein against Streptococcus mutans

2014 ◽  
Vol 49 (1) ◽  
pp. 78-89 ◽  
Author(s):  
Sug-Joon Ahn ◽  
Soon-Nang Park ◽  
Young Ju Lee ◽  
Eun-Jung Cho ◽  
Yun Kyong Lim ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Antimicrobial Activities ◽  
Licorice Root ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Root Extract ◽  
Gingival Fibroblast ◽  
Biofilm Development ◽  
Time Kill

The objective of the study was to investigate the antimicrobial effects of purified single compounds from ethanol-extracted licorice root on Streptococcus mutans. The crude licorice root extract (CLE) was obtained from Glycyrrhiza uralensis, which was subjected to column chromatography to separate compounds. Purified compounds were identified by mass spectrometry and nuclear magnetic resonance. Antimicrobial activities of purified compounds from CLE were evaluated by determining the minimum inhibitory concentration and by performing time-kill kinetics. The inhibitory effects of the compounds on biofilm development were evaluated using crystal violet assay and confocal microscopy. Cell toxicity of substances to normal human gingival fibroblast (NHGF) cells was tested using a methyl thiazolyl tetrazolium assay. Chlorhexidine digluconate (CHX) was used in the control group. Three antimicrobial flavonoids, 1-methoxyficifolinol, licorisoflavan A, and 6,8-diprenylgenistein, were isolated from the CLE. We found that the three flavonoids and CHX had bactericidal effects on S. mutans UA159 at the concentration of ≥4 and ≥1 µg/ml, respectively. The purified compounds completely inhibited biofilm development of S. mutans UA159 at concentrations over 4 μg/ml, which was equivalent to 2 μg/ml of CHX. Confocal analysis showed that biofilms were sparsely scattered in the presence of over 4 μg/ml of the purified compounds. However, the three compounds purified from CLE showed less cytotoxic effects on NHGF cells than CHX at these biofilm-inhibitory concentrations. Our results suggest that purified flavonoids from CLE can be useful in developing oral hygiene products, such as gargling solutions and dentifrices for preventing dental caries.

scholarly journals Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study

2020 ◽  
Author(s):  
Tatsuya Ohsumi ◽  
Shoji Takenaka ◽  
Yuuki Sakaue ◽  
Yuki Suzuki ◽  
Ryoko Nagata ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Combination Group ◽  
Control Group ◽  
Mouth Rinse ◽  
Biofilm Thickness ◽  
Log Reduction ◽  
Mouth Rinses ◽  
Significant Difference

Abstract Background: The aim of this in vitro study was to examine the possible enhancement of the biofilm peeling effect of a sonic toothbrush following the use of an antimicrobial mouth rinse.Methods: The biofilm at a noncontact site in the interdental area was treated by sound wave convection with the test solution or by immersion in the solution. The biofilm peeling effect was evaluated by determining the bacterial counts and performing morphological observations. A Streptococcus mutans biofilm was allowed to develop on composite resin discs by cultivation with stirring at 50 rpm for 72 hours. The specimens were then placed in recesses located between plastic teeth and divided into an immersion group and a combination group. The immersion group was treated with phosphate buffer, Peridex™ mouth rinse or Listerine® mouth rinse. The combination group was treated with Peridex™ or Listerine® and a sonic toothbrush.Results: The biofilm thickness was reduced by approximately one-half compared with the control group. The combination treatment produced a 1 log reduction in the number of bacteria compared to the Listerine® immersion treatment. No significant difference was observed in the biofilm peeling effect of the immersion group compared to the control group.Conclusions: The combined use of a sonic toothbrush and a mouth rinse enhanced the peeling of the biofilm that proliferates in places that are difficult to reach using mechanical stress.

scholarly journals EFEK ANTIBAKTERI SUPERNATAN JUS ANGGUR MERAH YANG DIISOLASI DENGAN KECEPATAN SENTRIFUGASI 12.000 rpm TERHADAP PERTUMBUHAN S. MUTANS

2016 ◽  
Vol 3 (2) ◽  
pp. 81
Author(s):  
Dini Rachmawati ◽  
Sumarno . ◽  
Anggi Wahyu Nur Cahyani
Keyword(s):  
Streptococcus Mutans ◽  
Brain Heart Infusion ◽  
Grape Juice ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Post Test ◽  
Red Grape Juice ◽  
Red Grape

Background: Streptococcus mutans (S.mutans) is a gram-positive bacteria in oral cavity which produced acid as the common cause of dental caries. In order to prevent the growth or kill Streptococcus mutans, antibacterial is required. Red grape (Vitis vinifera) is suspected to contain antibacterial.Method: An experimental study using the post test only control group design is carried out to Streptococcus mutans with tube dilution method and streaking on the Brain Heart Infusion. A range of concentration from 40%, 50%, 60%, 70%, 80%, 90%, 98%. The control group are group of bacteria and red grape juice control.Result: The analysis of Minimum Inhibitor Bactericidal (MIC) is show a difference absorbent value as the given concentration (Anova, p<0,05). The correlation test show is strong reciprocal association between concentration of res grape juice and absorbent value (r=-0,978; p< 0,05). The analysis of Minimum Bactericidal Concentration (MBC) is shows difference of the number of colonies as given concentration (Anova, p<0,05). The correlation reveals a very strong reciprocal association between concentration of red grape juice and the number of Streptococcus mutans colonies growth (r=-0,925; p< 0,05).Conclusion: The conclusion is supernatant of red grape juice has effect the growth as antibacterial agent on Streptococcus mutans in vitro, with MIC at concentration 60% and MBC at concentration 80%

scholarly journals PERBANDINGAN EFEKTIFITAS JUS LIDAH BUAYA DAN KLORHEXIDIN 0.06% TERHADAP JUMLAH KOLONI STREPTOCOCCUS MUTANS SALIVA ANAK DENGAN KARIES

2018 ◽  
Vol 5 (1) ◽  
pp. 54
Author(s):  
Shanty Chairani ◽  
Sri W Rais ◽  
Rani Purba ◽  
Amalia AH
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Aloe Vera ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Control Group ◽  
Mouth Rinse ◽  
Active Components ◽  
Control Group Design

Background: Aloe vera contains active components such as antrhaquinones,saponin, glucomannans, and acemannans that have been known for its antimicrobial properties. In vitro study showed that Aloe vera extract can inhibit the growth of Streptococcus mutans. Studies reported that children with caries have high level of salivary S. mutans which is the main pathogen in dental caries. The gold standard mouthrinse for dental caries is chlorhexidine gluconate, but it has several side effects. The objective of this study was to compare the effect between Aloe vera juice and 0.06% chlorhexidine mouth rinse on salivary S. mutans in children with caries.Methods: This experimental research with pretest and post-test control group design conducted on 30 children (6-12 years old) with number of carious teeth >4. Subjects divided into 2 groups: Aloe vera juice group and 0.06% chlorhexidine group, which instructed to rinse their mouth with 10 ml stated mouthwash, twice a day, during 7-day period. Unstimulated saliva was collected at baseline and after 7 days then inoculated on mitis salivarius bacitracin agar. S. mutans were counted and expressed in CFU/ml. Data were analyzed using paired t-test and independent t-test.Result: The result showed that a significant reduction in salivary S.mutanscounts was observed with Aloe vera juice mouth rinse and 0.06% chlorhexidine (p<0.05). The mean reduction of S. mutans counts was not statistically significantly between grup (p>0.05).Conclusion: These findings suggest that Aloe vera juice can reduce salivary S. mutans in children with caries so it can be considered as alternative mouthwash to prevent caries.

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