scholarly journals Karakteristik ikan bulan-bulan (Megalops cyprinoides) dan potensinya sebagai tepung untuk fortifikasi pangan

2020 ◽  
Vol 11 (2) ◽  
pp. 182-191
Author(s):  
Reni Tri Cahyani ◽  
Stephanie Bija ◽  
Leny Tang Nga Sugi
Keyword(s):  
Protein Content ◽  
Physiological Effects ◽  
Food Fortification ◽  
Processing Waste ◽  
Fortified Foods ◽  
Fresh Fish ◽  
Yellow Color ◽  
Two Stages ◽  
Powdered Form

Fish is very good for health because it contains various important nutrients such as protein, fat, and minerals. Food fortification uses fish’s sources is an effort to obtain physiological effects and improve the nutritional status of fortified foods. This study aims to examine the potential of Bulan-bulan fish (Megalops cyprinoides) as flour for food fortification. This research was divided into two stages, namely characterization of the fresh fish and preliminary analysis of its potential as fish flour. The data obtained were analyzed descriptively. The results showed that the edible portion of fresh fish was only 42.81%, whereas the remaining of 57.19% was processing waste. The fish had a high protein content of 21.43%, fat content was 13.32%, ash content was 12.80%, moisture content was 42.29% and carbohydrate content was 10.19%. The yield of the fish flour was 22.75%, with sensory characteristics: powdered form; clean, normal, and bright appearance; bright brownish yellow color; specific aroma; non-lumpy and quite dry texture. The Protein content of the fish flour was 55.44% which comply with the protein’s requirement of quality II fish flour.

KARAKTERISTIK ABON IKAN GABUS (Channa striata) DENGAN SUBTITUSI KLUWIH (Artocarpus camansi)

2018 ◽  
Vol 16 (2) ◽  
pp. 219-226
Author(s):  
Sri Rizqi Annisa ◽  
Dewi Larasati ◽  
Endang Bekti K
Keyword(s):  
Protein Content ◽  
Water Content ◽  
Analysis Of Variance ◽  
Fiber Content ◽  
Substitution Treatment ◽  
Fish Fillet ◽  
Organoleptic Test ◽  
Average Water ◽  
Channa Striata

The aim of this study was to determine the characterization of shredded mureel fish with kluwih substitution on water content, protein content, fiber content and organoleptic (preference for crispness and taste). This study uses a simple Randomized Complete Design (RCD) with the substitution treatment of kluwih and mureel fish, with the following ratio: S1 (240g: 60g), S2 (210g: 90g), S3 (180g: 120g), S4 (150g: 150g), S5 (120g: 180g). Data were analyzed statistically by analysis of variance and if there was a significant effect, further testing was done with BNJ at the level of 5%. The results showed that kluwih substitution in the manufacture of mureel fish shredded had an average: water content of 8.33-10.62%, protein :16.83-22.00%, fiber : 6.79-6.99%, score crispness 2-6.12, taste score 2.6-6.6. Based on the results of the analysis of the variety of kluwih substitutes and mureel fish have a significant effect on water content, protein content and crisp organoleptic test, taste on mureel fish fillet, and no significant effect on fiber content. The best kluwih substitution in S3 treatment with 120 grams of substitute kluwih and 180 grams of mureel fish.

scholarly journals Mass Spectrometry-Based Proteomic Characterization of Cutaneous Melanoma Ectosomes Reveals the Presence of Cancer-Related Molecules

2020 ◽  
Vol 21 (8) ◽  
pp. 2934 ◽  
Author(s):  
Magdalena Surman ◽  
Sylwia Kędracka-Krok ◽  
Dorota Hoja-Łukowicz ◽  
Urszula Jankowska ◽  
Anna Drożdż ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Cell Lines ◽  
Cutaneous Melanoma ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Starting Point ◽  
Novel Biomarkers

Cutaneous melanoma (CM) is an aggressive type of skin cancer for which effective biomarkers are still needed. Recently, the protein content of extracellular vesicles (ectosomes and exosomes) became increasingly investigated in terms of its functional role in CM and as a source of novel biomarkers; however, the data concerning the proteome of CM-derived ectosomes is very limited. We used the shotgun nanoLC–MS/MS approach to the profile protein content of ectosomes from primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cell lines. Additionally, the effect exerted by CM ectosomes on recipient cells was assessed in terms of cell proliferation (Alamar Blue assay) and migratory properties (wound healing assay). All cell lines secreted heterogeneous populations of ectosomes enriched in the common set of proteins. A total of 1507 unique proteins were identified, with many of them involved in cancer cell proliferation, migration, escape from apoptosis, epithelial–mesenchymal transition and angiogenesis. Isolated ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of different cancer-promoting molecules. Taken together, these results confirm the significant role of ectosomes in several biological processes leading to CM development and progression, and might be used as a starting point for further studies exploring their diagnostic and prognostic potential.

Molecular characterization of the goat CSN1S101 allele

2003 ◽  
Vol 70 (2) ◽  
pp. 237-240 ◽  
Author(s):  
Gianfranco Cosenza ◽  
Rosa Illario ◽  
Andrea Rando ◽  
Paola di Gregorio ◽  
Piero Masina ◽  
...  
Keyword(s):  
Protein Content ◽  
Molecular Characterization ◽  
Single Copy ◽  
Chromosome 6

Caseins (αs1, β, αs2, e κ) represent about 80% of the whole protein content of ruminant milk. Each of these proteins is encoded by single copy genes (CSN1S1, CSN2, CSN1S2 and CSN3, respectively) clustered on a ∼200-kb segment of chromosome 6 (Ferretti et al. 1990; Gallagher et al. 1994) in the order: CSN1S1, CSN2, CSN1S2 and CSN3 (Mercier & Vilotte, 1993). Furthermore, in cattle and goat CSN1S1 and CSN2 are convergently transcribed (Leroux & Martin, 1996; Rijnkles et al. 1997) and are only 20 and 12 kb apart, respectively.

Growth mechanism and characterization of ZnO nano-tubes synthesized using the hydrothermal-etching method

2009 ◽  
Vol 63 (6) ◽  
Author(s):  
Yan Li ◽  
Chuan-Sheng Liu ◽  
Yun-Ling Zou
Keyword(s):  
Room Temperature ◽  
Morphological Analysis ◽  
Diffraction Field ◽  
X Ray Diffraction ◽  
X Ray ◽  
Room Temperature Photoluminescence ◽  
Etching Method ◽  
Nano Rods ◽  
Two Stages

AbstractZnO nano-tubes (ZNTs) have been successfully synthesized via a simple hydrothermal-etching method, and characterized by X-ray diffraction, field emission scanning electron microscopy and room temperature photoluminescence measurement. The as-synthesized ZNTs have a diameter of 500 nm, wall thickness of 20–30 nm, and length of 5 µm. Intensity of the plane (0002) diffraction peak, compared with that of plane (10$$ \bar 1 $$0) of ZNTs, is obviously lower than that of ZnO nano-rods. This phenomenon can be caused by the smaller cross section of plane (0002) of the nano-tubes compared with that of other morphologies. On basis of the morphological analysis, the formation process of nano-tubes can be proposed in two stages: hydrothermal growth and reaction etching process.

The Experimental of Low Temperature Color Slip for Decorative on Earthenware Body

2016 ◽  
Vol 690 ◽  
pp. 282-285
Author(s):  
Soravich Mulinta
Keyword(s):  
Low Temperature ◽  
Physical Properties ◽  
Manganese Oxide ◽  
Ferric Oxide ◽  
Potassium Feldspar ◽  
Raw Material ◽  
Blue Color ◽  
X Ray ◽  
Yellow Color

The purpose of the study was to investigate the effects of dolomite body, frit and potassium feldspar on the properties of color slip for decorative method of color slip on earthen ware production. The characterization of raw material was analyzed by X-ray fluorescence (XRF). The experiment started with triaxial blend of dolomite body, frit and potassium feldspar in the defined ratio and then to painting of color slips on earthenware production. After that, firing specimens at 950 °C in oxidation atmosphere. Finally, the specimens were tested microstructure and physical properties. The results showed that the ratio of 50% dolomite body, 40% frit and 10% potassium feldspar were optimum properties for decorative on earthenware body. In color slip consisting of blue color added Cobalt oxide 1% ,Green colors added chromic oxide 10% , Gray color added manganese oxide 10% ,yellow color added stain 2225 10% and hazel color added ferric oxide 2 wt %.

WILD PEAVINE SPECIES (LATHYRUS L.): CHARACTERIZATION OF ACCESSIONS IN TERMS OF PROTEIN CONTENT IN THEIR SEEDS AND GREEN BIOMASS

2020 ◽  
Author(s):  
M.O. BURLYAEVA ◽  
◽  
A.E. SOLOVEVA ◽  
E.A. SERGEEV ◽  
N.I. TOPILSKAYA ◽  
...  
Keyword(s):  
Protein Content ◽  
Grain Legumes ◽  
Natural Habitats ◽  
Fodder Crops

The catalogue presents the results of the assessment of protein content in green biomass and seeds of 367 accessions from the VIR collection, representing 25 wild peavine species, during their introduction into Ukraine and into Leningrad and Tambov Provinces of Russia. Besides, it includes the data on the protein content in green biomass of 65 accessions, representing 12 wild peavine species, collected in their natural habitats in Russia and China. The catalogue is addressed to experts in fodder crops and grain legumes.

Characterization of nuclear structures containing superhelical DNA

1976 ◽  
Vol 22 (2) ◽  
pp. 303-324 ◽  
Author(s):  
P.R. Cook ◽  
I.A. Brazell ◽  
E. Jost
Keyword(s):  
Protein Content ◽  
Ionic Detergents ◽  
High Concentrations ◽  
Nuclear Structures ◽  
Intercalating Agents ◽  
Made In

Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.

scholarly journals EVQuant; high-throughput quantification and characterization of extracellular vesicle (sub)populations

2020 ◽  
Author(s):  
T.A. Hartjes ◽  
J.A. Slotman ◽  
M.S. Vredenbregt ◽  
N. Dits ◽  
R. Van der Meel ◽  
...  
Keyword(s):  
Protein Content ◽  
Size Distribution ◽  
High Throughput ◽  
Liquid Biopsy ◽  
Ideal Liquid ◽  
Extracellular Vesicle ◽  
Cell Of Origin ◽  
Clinical Implementation ◽  
High Throughput Assay

AbstractExtracellular vesicles (EVs) reflect the cell of origin in terms of nucleic acids and protein content. They are found in biofluids and represent an ideal liquid biopsy biomarker source for many diseases. Unfortunately, clinical implementation is limited by available technologies for EV analysis. We have developed a simple, robust and sensitive microscopy-based high-throughput assay (EVQuant) to overcome these limitations and allow widespread use in the EV community. The EVQuant assay can detect individual immobilized EVs as small as 35 nm and determine their concentration in biofluids without extensive EV isolation or purification procedures. It can also identify specific EV subpopulations based on combinations of biomarkers and is used here to identify prostate-derived urinary EVs as CD9-/CD63+. Moreover, characterization of individual EVs allows analysis of their size distribution. The ability to identify, quantify and characterize EV (sub-)populations in high-throughput substantially extents the applicability of the EVQuant assay over most current EV quantification assays.

scholarly journals Nutritional Characterization of a Traditional Cultivar of Tomato Grown Under Organic Conditions—cv. “Malacara”

2022 ◽  
Vol 8 ◽  
Author(s):  
María D. Raigón ◽  
María D. García-Martínez ◽  
Octavian P. Chiriac
Keyword(s):  
Organic Farming ◽  
Nutritional Value ◽  
Fruit Weight ◽  
Organic Production ◽  
Local Varieties ◽  
Yellow Color ◽  
Organic Farmers ◽  
Tomato Cultivars ◽  
Selection Of

The loss of genetic diversity due to the replacement of local tomato (Solanum lycopersicum L.) varieties by improved cultivars has been mitigated in many cases by the good work of organic farmers in maintaining local agricultural biodiversity. In parallel to these initiatives, in recent years, consumers have developed an increasing awareness of both food-related health, environmental issues, and food demand to recover the flavors of the past. In the case of tomatoes, these attributes (nutritional, organoleptic, social, and environmental) are closely related to organic production using local varieties. “Malacara” tomato is an example of a local variety. Coming from Sierra de Cádiz, it is a varietal type called “Cuelga” (“for hanging,” because the tomato trusses are hung from beams in the farmhouses). Cultivated and harvested in the open air during the summer months, these tomatoes are commercialized and consumed in the winter. Historically, this variety has enabled the fresh consumption of tomatoes during the winter, without the need to force cultivation. It is highly appreciated in the local cuisine and is the basis for sauces figuring in typical dishes. Its characteristic traits are small, pallid fruits, and long shelf life. The main objective of this work has been to typify two Malacara tomato cultivars (red and yellow color) grown under organic farming conditions, through the characterization of morphological, nutritional, and volatile parameters. The main differences are due to morphological parameters (fruit weight and color of the exocarp and endocarp). Other characteristics such as the content of ash, fiber, moisture, the concentration of iron, magnesium, and calcium, and content of lycopene are different between both cultivars. This study provides information on the nutritional and aromatic composition of two Malacara tomato cultivars, differentiated by their color and grown under organic farming conditions. The results add value to the native horticultural heritage and can aid in the selection of tomato varieties suitable for a sustainable production system and to produce tomatoes with high nutritional value and rich in aroma.

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