scholarly journals The Role of Bidara Leaf Extract (Ziziphus Mauritiana) on the Prevention of Renal Hypertension in Wistar Strain Rats

2020 ◽  
Vol 9 (3S) ◽  
pp. 298-300
Keyword(s):  
Blood Pressure ◽  
Group Treatment ◽  
Leaf Extract ◽  
Renal Hypertension ◽  
Positive Control ◽  
Control Group ◽  
Ziziphus Mauritiana ◽  
Control Group Design ◽  
White Rats ◽  
Wistar Strain

Hypertension is ranked the fourth highest disease in the word. Glomerulosclerosis is the initial lesion from kidney failure in patient with hypertension. Bidara leaves have active flavonoid such as saponins and tannins are thought to prevent an increase in blood pressure and repair glomerulosclerosis. This study to prove the effect of bidara leaf extract to decrease hypertension and to prevent glomerulosclerosis. This study was true experimental with Pre and Post Test Control Group Design. This study used male white rats wistar strain. Sample divided into 5 groups (positive group, treatment group with 3 leveled dose, and medicine-control group) and were being observed in 28 days. Hypertension was measured using tail cuff blood pressure. Glomerulus observed randomly with light microscope in 5 different field of view with 400x magnification and scoring by Sclerosis Index (SI). In the linear regression test had R 2 = 0,332 for systolic blood pressure and R 2 = 0,609 for diastolic blood pressure. For glomerulosclerosis we found that the highest SI (369,69) was positive control group, while the lowest SI (84,09) was the group with a dose 400mg/kg/day. Bidara leaves extract have a significant effect on reduce hypertension and glomerulosclerosis.

scholarly journals Effect of Jati Belanda Leaves Extract on Myeloperoxidase Level in Wistar Rat's Lung Induced Contusion Pulmonum

2019 ◽  
Vol 5 (3) ◽  
pp. 116-120
Author(s):  
Nia Savitri Tamzil ◽  
Evi Lusiana ◽  
Desi Oktariana
Keyword(s):  
Leaf Extract ◽  
Positive Control ◽  
Lung Damage ◽  
Control Group ◽  
Alveolar Cells ◽  
Pulmonary Tissue ◽  
Control Group Design ◽  
White Rats ◽  
Guazuma Ulmifolia

Pulmonary contusions are injuries to the lung parenchyma that often result from blunt trauma to the chest wall. This injury will activate the inflammatory response which can produce the effects of oxidative stress so that eventually lung damage occurs. Several studies have identified the effects of Jati Belanda leaves extracts related to the inflammatory process and their effects as antioxidants. This research is an in vivo experimental study with a prepost-test with control group design approach that aims to determine the effectiveness of the extracts of Jati Belanda (Guazuma ulmifolia) in its protection against pulmonary alveolar cells by pretreatment pulmonary contusions. The subjects of this study were white rats (Rattus norvegicus) Wistar strain which were divided into 5 groups namely positive control, negative control, Dutch teak leaf extract 125 mg / kgBB, 250 mg / kgBB and 500 mg / kgBW. Rats were induced by dropping a weight of 400 grams as high as 50 cm. The results of the study of Jati Belanda Leaf Extract (EDJB) all doses and positive control can significantly reduce levels of myeloperoxidase expression (p <0.05). Therefore, it can be concluded that the extracts of Jati Belanda leaves (Guazuma ulmifolia) are all effective doses in reducing the expression of myeloperoxidase in pulmonary tissue of Wistar rats induced by pulmonary contusions.

scholarly journals Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica)  is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

scholarly journals EFFECTIVENESS OF LEAF EXTRACT REDUCE INFLAMMATION REACTION IN HYPERCHOLESTEROLEMIA RATS

2017 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Puguh Santoso ◽  
Ni Nyoman Wahyu Udayani ◽  
I Nyoman Gede Tri Sutrisna ◽  
Ketut Agus Adrianta
Keyword(s):  
Free Radicals ◽  
Leaf Extract ◽  
Oxidized Ldl ◽  
Oxidative Modification ◽  
Blood Cholesterol ◽  
Ldl Oxidation ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Group I

Abstract High blood cholesterol is often called hypercholesterolemia is a risk factor for the emergence of pathological conditions such as heart and blood vessel disease. Hypercholesterolemia has an important role in the occurrence of damage to the endothelial cells is mainly caused by oxidized LDL. Oxidation of LDL triggers the formation of TNF - ?. Leaves messengers that allegedly contains flavonoids can improve the situation of hypercholesterolemia through the barriers specifically the expression of TNF - ? increased due to hypercholesterolemia. Plants messengers known to contain alkaloids, flavonoids, tannins, saponins, polyphenols, calcium oxalate, fats, and essential oil. Flavonoids which has the ability to bind the atom to form free radicals not to excess free radicals, thereby inhibiting the oxidative modification of LDL become ox-LDL so it will not be formed atherosclerosis. This study uses the Randomize pattern Pre and Post Test Control Group Design, using white rats (Rattusnovergicus) with Wistar strain aged 3-4 months, weighing 175-200 grams. Divided into four groups: Group I as a control with placebo, Group II treatment using extracts of ethanol leaves a messenger at a concentration of 10%, Group III treatments using extracts of ethanol leaves a messenger at a concentration of 20%, Group IV is to use the extract ethanol leaves errand at a concentration of 30%. It can be concluded at 4 dose group 30% messengers leaf extract significantly different, p <0.05, so it can be said that the provision of effective messengers extract at a dose of 30%.

ANTI-HYPERCHOLESTEROLEMIA ACTIVITY OF ETHANOL EXTRACT Peperomia pellucida

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Chasanah Mazroatul
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diet Group ◽  
In Vivo Studies ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Control Group Design ◽  
White Rats

<p>Hypercholesterolemia is a major cause of cardiovascular disease such as coronary heart disease. Betel water (Peperomia pellucida) is a type of plants that have antioxidant compounds that could delay, retard and prevent the oxidation of lipids, both enzymatic and non-enzymatic. This study aimed to determine the effect of ethanol extract Peperomia pellucida against total cholesterol, HDL, LDL, and triglycerides in the serum of white rats (Wistar) were given a diet aterogenetik, so it can be used as prevention of atherosclerosis. The active compounds contained in the water after screnning betel phytochemicals includes flavonoids, tannins, alkaloids, steroids and quinones. In vivo studies conducted by true experimental method with pre and post test with control group design. Rats were divided into 3 groups: group A positive control is given aterogenetik diet, group B and C were given diet Peperomia Pellucida aterogenetik and extract orally at a dose of 150 mg/kg and 300 mg/kg. Diet aterogenetik given as much as 20 grams per day for 14 days. Data obtained include total cholesterol, HDL, LDL, and triglycerides were analyzed by statistical methods Paired T Test oneway ANOVA (P &lt; 0,05). The study of total cholesterol, HDL, LDL and triglycerides showed ethanol extract of Peperomia pellucida at a dose of 300 mg/kg body weight can lower total cholesterol and LDL significantly, but there was no significant decline in triglycerides and can increase HDL levels.</p>

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals PERBEDAAN EFEKTIVITAS EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorriza Roxb.) DENGAN EKSTRAK DAUN SALAM (Eugenia polyantha Wight) PADA PENURUNAN KADAR KOLESTEROL TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Ayu Ulan Rizki ◽  
Cholid Cholid ◽  
Muttia Amalia
Keyword(s):  
Total Cholesterol ◽  
Leaf Extract ◽  
Disease Risk ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Standard Drug ◽  
Cholesterol Levels ◽  
White Rats ◽  
Ginger Rhizome

Dyslipidemia is a coronary heart disease risk factor. Dyslipidemia is characterized by the elevated levels of LDL, triglycerides, total cholesterol, and the decrease in HDL. Ginger rhizome (Curcuma xanthorriza Roxb.) and bay leaf (Eugenia polyantha Wight.) are local plants that have the abilities to decrease total cholesterol. This research design was true experimental with pre- and post-control group design. Criteria of sample were white rats, wistar strain, male, age of 8-12 weeks, and body weight range of 150-200 grams. Sampling method was simple random sampling. The experimental rats were acclimatized for seven days. Hypercholesterol diet was fed two weeks, and then the rats were given intervention and get hypercholesterol diet for two weeks. During intervention, the experimental rats were divided into 6 groups of administrationi.e, rats administered with 1) carboxymethil celluse/CMC, 2) simvastatin 0,9 mg/kg BW/day, 3) ginger rhizome extract 200 mg /kg BW/day, 4) ginger rhizome extract 400 mg/kg BW/day, 5) bay leaf extract 200 mg/kg BW/day, and 6) bay leaf extract 400 mg/kg BW/day. Blood samples were taken from caudal vein and total cholesterol levels were measured by spectrophotometry. The analysis used paired T test, One Way ANOVA test. The results showed ginger rhizome extract did not effectively reduce total cholesterol. However, bay leaf extract effectively decreased total cholesterol levels with the highest total cholesterol reduction was found in rats administered with dose of 400 mg/kg BW/day i.e, 15.4 mg/dl. Bay leaf extract have the same effectivity with simvastatin as a standard drug in lowering cholesterol level.

scholarly journals THE EFFECTIVENESS OF CORIANDER (Coriandrum sativum L.) EXTRACTS ON REDUCING THE BLOOD SUGAR LEVELS OF ALLOXAN INDUCED WISTAR MALE RATS (Rattus norvegicus L.)

2020 ◽  
Vol 6 (1) ◽  
pp. 36-44
Author(s):  
Kalvin Dersing
Keyword(s):  
Blood Sugar ◽  
Rattus Norvegicus ◽  
Water Extract ◽  
Coriandrum Sativum ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Wistar Strain ◽  
Sugar Levels

AbstractBackground : Diabetes mellitus is the most common metabolic syndrome in the whole world with an incidence of 1-8%. This disease arises when insufficient insulin is produced or insulin cannot function properly.The ability of coriander juice water extract is expected to reduce blood glucose levels because the action is similar to insulin and can stimulate insulin release. Purpose : Knowing effectiveness coriander extract (Coriandrum sativum L.)to decline blood sugar levels in male white rats (Rattus norvegicus L.) wistar strain induced by alloxan. Method : This is a pure experimental study (true-experiment) using a pre and post test design with control group design using subjects namely white rats (Rattus norvegicus L.) male Wistar strain aged 10-12 weeks with a weight of 150-200 grams. By looking at changes in blood sugar levels during administration of alloxan and administration of coriander extract. Results : There were significant changes in some groups induced alloxan (p <0.05), but the effect of coriander extract was not very significant (p> 0.05). Conclusion : Coriander extract can control blood sugar levels to be stable or normal, but it takes longer and more research.  Keywords : coriander extract, blood sugar levels, alloxan

scholarly journals ANTI-HYPERCHOLESTEROLEMIA ACTIVITY OF ETHANOL EXTRACT Peperomia pellucid

2016 ◽  
Vol 12 (1) ◽  
pp. 88 ◽  
Author(s):  
Chasanah Mazroatul ◽  
Glar Donia Deni ◽  
Nur Ahmad Habibi ◽  
Gita Febri Saputri
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diet Group ◽  
In Vivo Studies ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Control Group Design ◽  
White Rats

Hypercholesterolemia is a major cause of cardiovascular disease such as coronary heart disease. Betel water (Peperomia pellucida) is a type of plants that have antioxidant compounds that could delay, retard and prevent the oxidation of lipids, both enzymatic and non-enzymatic. This study aimed to determine the effect of ethanol extract Peperomia pellucida against total cholesterol, HDL, LDL, and triglycerides in the serum of white rats (Wistar) were given a diet aterogenetik, so it can be used as prevention of atherosclerosis. The active compounds contained in the water after screnning betel phytochemicals includes flavonoids, tannins, alkaloids, steroids and quinones. In vivo studies conducted by true experimental method with pre and post test with control group design. Rats were divided into 3 groups: group A positive control is given aterogenetik diet, group B and C were given diet Peperomia Pellucida aterogenetik and extract orally at a dose of 150 mg/kg and 300 mg/kg. Diet aterogenetik given as much as 20 grams per day for 14 days. Data obtained include total cholesterol, HDL, LDL, and triglycerides were analyzed by statistical methods Paired T Test oneway ANOVA (P &lt; 0.05). The study of total cholesterol, HDL, LDL and triglycerides showed ethanol extract of Peperomia pellucida at a dose of 300 mg/kg body weight can lower total cholesterol and LDL significantly, but there was no significant decline in triglycerides and can increase HDL levels.

scholarly journals The influence of injection of depot medroxy progesterone acetate (DMPA) to the gland tissue histology breast on white rats breastfeeding

2018 ◽  
Vol 12 (2) ◽  
pp. 94
Author(s):  
Asmita Dahlan
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Female Rats ◽  
White Female ◽  
Control Group ◽  
Control Group Design ◽  
Pathology Laboratory ◽  
White Rats ◽  
Gland Tissue ◽  
Tissue Histology

<p><em>Depo MedroxyProgesteroneAcetate</em> (DMPA) allegedly have the effect of breast glandular tissue breast-feeding women, and to prove it then conducted research into how the influence of injection of DMPA to the gland tissue histology breast on white female rats breastfeeding.This research is experimental research design with post test only control group design that was implemented starting carried out in laboratories of pharmaceutical Faculty of Science and anatomic pathology laboratory andalas university medical school fields, the total sample 27 white female rats lactating drawn at random by age 2-3 months, weight 160-200 grams, divided into 3 groups consisting of the control group, treatment group 1 (DMPA 150 mg) and treatment group 2 (DMPA 300 mg). DMPA injections done in intra muscular  every 5 days during 4x injections (20 days). Research data were statistically processed using anova test with 95% confidence. Results showed that there are differences in the development of lobulus and asinus in the control group and treatment group.Conclusions of this study is the effect of DMPA injections in female rats breastfeeding on the area and the development of lobular breast lobes as well as breast acini.</p>

scholarly journals Effects of Dexamethasone on Contralateral Testicular Immune Response in Wistar Strain Rats with Unilateral Testicular Torsion

2018 ◽  
Vol 54 (1) ◽  
pp. 29
Author(s):  
Andri Kustono ◽  
I Ketut Sudiana ◽  
Fikri Rizaldi ◽  
Tarmono Tarmono
Keyword(s):  
Testicular Torsion ◽  
Sham Group ◽  
Positive Control ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Wistar Strain ◽  
Post Test ◽  
The Right

The aim of this study was to prove the effects of dexamethasone on immune responses in rats with testicular torsion. This was an experimental study with post test only control group design. In this study, 27 rats were randomly divided into 3 groups. In normal or sham group (CN) orchidectomy was performed on the right testis on day 14. The positive control group (CP) had testicular torsion for 10 h, then manual detorsion was performed after 4 h, and on day 14 orchidectomy was performed on ipsilateral and contralateral testes. The treatment group (N) had testicular torsion for 10 h and received 10 mg/kgBW dexamethasone subcutaneously 30 minutes before manual detorsion 4 hours later, and on day 14 orchidectomy was performed to ipsilateral and contralateral testes. The spermatogonium count was calculated based on the average number of intratubular seminiferous tubule section observed in contralateral testicular incision with Haematoxiliyn Eosin (HE) staining. IgG count was calculated based on the average number of extratubular seminiferous tubules observed at 5 contralateral testicular incision with immunohistochemical staining (IHC). The number of spermatogonia was significantly different between CN and CP, CN and N, and CP and N (p<0.05). IgG production also showed significant differences between CN and CP, CN and N, as well as CP and N (p<0.05). In conclusion, administration of dexamethasone provides a protective effect on spermatogonium count and IgG production on a 10-hour testicular torsion.

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