scholarly journals The influence of injection of depot medroxy progesterone acetate (DMPA) to the gland tissue histology breast on white rats breastfeeding

2018 ◽  
Vol 12 (2) ◽  
pp. 94
Author(s):  
Asmita Dahlan
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Female Rats ◽  
White Female ◽  
Control Group ◽  
Control Group Design ◽  
Pathology Laboratory ◽  
White Rats ◽  
Gland Tissue ◽  
Tissue Histology

<p><em>Depo MedroxyProgesteroneAcetate</em> (DMPA) allegedly have the effect of breast glandular tissue breast-feeding women, and to prove it then conducted research into how the influence of injection of DMPA to the gland tissue histology breast on white female rats breastfeeding.This research is experimental research design with post test only control group design that was implemented starting carried out in laboratories of pharmaceutical Faculty of Science and anatomic pathology laboratory andalas university medical school fields, the total sample 27 white female rats lactating drawn at random by age 2-3 months, weight 160-200 grams, divided into 3 groups consisting of the control group, treatment group 1 (DMPA 150 mg) and treatment group 2 (DMPA 300 mg). DMPA injections done in intra muscular  every 5 days during 4x injections (20 days). Research data were statistically processed using anova test with 95% confidence. Results showed that there are differences in the development of lobulus and asinus in the control group and treatment group.Conclusions of this study is the effect of DMPA injections in female rats breastfeeding on the area and the development of lobular breast lobes as well as breast acini.</p>

scholarly journals PENGARUH PEMBERIAN AIR SEDUHAN BERAS YANG DIFERMENTASI OLEH MONASCUS PURPUREUS (ANGKAK) TERHADAP PENURUNAN KADAR KOLESTEROL SERUM PADA TIKUS PUTIH

Biomedika ◽  
2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Adhimass Wicaksono ◽  
Retno Sintowati ◽  
Sa’idatul Fitriyah
Keyword(s):  
Treatment Group ◽  
Serum Cholesterol ◽  
Brown Rice ◽  
Negative Control ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Cholesterol Levels ◽  
White Rats ◽  
Group A

The using of plant-based medicine is a popular approach to health care. A study presented by American Hearth Association showing that brown rice can lower cholesterol level in bood serum. To know the benefi ts of Angkak’s steeping water to decrease serum cholesterol levels in mice and determine the effects of Angkak’s steeping water to decrease serum cholesterol levels in rats compared with simvastatin. This study used an experimental research design, and using pre and post test control group design, performed in the laboratory of biomedical III FK UMS on November 1, 2012 to date of January 1, 2013. The purposive sampling technique, sampel determinaton using formula Federer by the number of sample of 30 male white rats wistar strain. After the data is collected, analyzed using the Shapiro-Wilk test, ANOVA, LSD, and the unpaired t test. The result of this study demnstrate hypothesis testing using ANOVA test with signifi cant level 95% of the negative control group, the treatment group a dose of 1, 2, and 3, it was found p<0,05 were signifi cantly different meaning. In the test dose of LSD between treatment groups 2 and 3 the value of p=0,839 (p>0,05) wich means no signifi cant difference. In the unpaired T test between the treatment group a dose of 1, 2, and 3 with positive control group were signifi cant difference. The unpaired T test between the treatment group a dose of 1, 2, and 3 with white rice also found signifi cant difference. Conclusion: Angkak’s steeping water can lower serum cholesterol levels of white rat as well as the water steepimg Angkak has the same effectiveness with simvastatin to decrease serum cholesterol levels of mice.Keyword: Cholesterol, Angkak

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Short Communication: Effects of black soy phytoestrogens (Glycine soja) on elevated levels of estradiol in rat blood (Rattus novergicus) ovariectomy

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Dwi Yuliawati ◽  
WURI WIDI ASTUTI ◽  
FITRI YUNIARTI
Keyword(s):  
Group Treatment ◽  
Rattus Norvegicus ◽  
Glycine Soja ◽  
Negative Control ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Rat Blood

Abstract. Yuliawati D, Astuti WW, Yuniarti F. 2020. Effects of Black Soy phytoestrogens (Glycine soja) on elevated levels of estradiol in Rat Blood (Rattus norvegicus) ovariectomy. Nusantara Bioscience 12: 55-58. Menopause is the transition from productive to non-productive times due to reduced estrogen and progesterone and has an impact on improving cardiovascular problems. The fulfillment of estrogen needs in menopause can use a type of phytoestrogens from black soybeans. The purpose of research is to prove the administration of black soy extract (Glycine soja) can increase the levels of estradiol in the rat blood (Rattus norvegicus) ovariectomy. Design research uses post-test only control group design. Twenty-five female rats were randomly divided into 5 groups (n = 5 tails per group), negative controls, positive and 3 treatments. Ovariectomy was performed in a group of positive controls and treatment. Thirty-day post ovariectomy rats were given appropriate group treatment (negative control, positive, and treatment with the administration of black soy extract dose of 50, 100, 150 mg/200  g/day for 30 days). The estradiol test used the Enzyme-linked immunosorbent assay (ELISA). Anova's test results with a significant degree of α = 0.05 established that the p-value of 0.000 was less than α = 0.05, meaning there was a significant effect of giving black soy extract on increased levels of estradiol in the rat blood ovariectomy. Post - Hoc Tukey testing shows increased the highest estradiol levels in the dose treatment group 100 mg/200  g/day. This research proves that the administration of black soy extract (Glycine soja) can increase the levels of estradiol in the rat blood (Rattus norvegicus) ovariectomy.

scholarly journals Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Idola Perdana Sulistyoning Suharto
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference ◽  
Phaleria Macrocarpa ◽  
Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound 

scholarly journals Antidiabetes Serbuk Umbi Tanaman Yakon (Smallanthus sonchifolius) pada Kadar Gula Darah Tikus Putih (Rattus norvegicus)

2021 ◽  
Vol 7 (1) ◽  
pp. 56-62
Author(s):  
Thatit Nurmawati ◽  
Yeni Kartika Sari ◽  
Fina Ayu Ningtyas
Keyword(s):  
Treatment Group ◽  
Blood Sugar ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Glucose Levels ◽  
Smallanthus Sonchifolius ◽  
White Rats ◽  
Comparison Results ◽  
Sugar Levels

The routine and long-term use of oral antidiabetic drugs in DM patients can make the public's perception worried about the side effects of the drug. In the Yakon (SS) plant tuber there is Fructooligosacaraide (FOS) which can reduce blood sugar levels. The research objective was to determine the effect of Yakon plant tubers on blood sugar levels in white rats with type 2 diabetes mellitus model. This type of research was experimental with a pre-post test design with control-group design. Independent variable, administration of Yakon plant tuber powder, dependent variable, blood glucose levels. The sample was divided into 2 groups (dose 1, dose 2) and a control group, each of 6 rats. The mice were given a high carbohydrate diet for 9 weeks. Treatment dose 1 (100 mg / kgBB) for 3 days. Measurement of blood sugar levels using Gluco-Dr. Data analysis used Paired Sample T-test, Independent Sample T-test, and One Way Anova. The results showed that the rats in the treatment group dose 1 experienced a decrease in the average blood sugar level of 122.83 mg / dl (p 0.002) (p 0.009). This shows that there is an effect of using Yakon plant tuber powder on blood sugar levels. The results of this study were the control group and the treatment group dose 1 (Sig. (2-tailed) = 0.000, α = 0.05). Meanwhile, the comparison results showed that there was a difference between the control group and the treatment group at dose 1, Based on the above research, Yakon tubers can be used to reduce blood sugar levels.

scholarly journals Pemberian makanan pengganti cair Nutrisure Gold® tidak meningkatkan kadar hormon estrogen tetapi meningkatkan kadar hormon progesteron pada anak tikus putih (Rattus norvegicus) galur Wistar betina

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Setia Wardani ◽  
Wimpie Pangkahila ◽  
IGM Aman
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Female Rats ◽  
Progesterone Level ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Estrogen Level ◽  
Post Test ◽  
Female Wistar Rats

Abstract: One of the factors that influences aging process is hormonal imbalance that can occur due to the intake of food or drink containing hormone. Children aged more than 1 year with difficulty in eating are usually given Nutrisure Gold®. Analysis has shown that Nutrisure Gold® contains estrogen dan progesterone. This study was aimed to prove that Nutrisure Gold® could increase estrogen and progesterone levels in Wistar female rats. This was an experimental study with the post test only control group design. Subjects were 36 female Wistar rats (Rattus norvegicus) aged 21 days divide into 2 groups, each of 18 rats. The control group was given white rice 3.11 g and aqua bidest meanwhile the treatment group was given Nutrisure Gold® 0.93 g three times a day for 28 days. The results showed that the average estrogen level in the control group was 44.26±11.49 pg/ml and of the treatment group was 45.30±7.66 pg/ml. The average progesterone level in the control group was 22.43±3.62 ng/ml and of the treatment group was 27.44±2.28 ng/ml. Data of estrogen level were analyzed with t-independent test that showed t = -0.320 and P = 0.751 (P >0.05) meanwhile of progesterone level showed t = -4.978 and P = 0.000 (P <0.05). Conclusion: After treatment, the average estrogen levels in both groups did not differ significantly meanwhile the average progesterone levels in both groups showed significant difference.Keywords: liquid replacement meal, estrogen, progesteroneAbstrak: Salah satu faktor yang memengaruhi proses penuaan ialah ketidak seimbangan hormon yang dapat terjadi antara lain akibat pemberian asupan makanan atau minuman yang mengandung hormon. Anak-anak berusia satu tahun ke atas dengan masalah sulit makan sering diberikan makanan pengganti cair Nutrisure Gold®. Analisis menunjukkan bahwa Nutrisure Gold® mengandung hormon estrogen dan progesteron. Penelitian ini bertujuan untuk membuktikan bahwa pemberian Nutrisure Gold® meningkatkan kadar hormon estrogen dan progesteron pada tikus putih galur Wistar betina umur 21 hari. Jenis penelitian ialah eksperimental dengan post test only control group design, Subyek penelitian ialah 36 ekor tikus putih (Rattus norvegicus) galur Wistar betina umur 21 hari yang dibagi atas 2 kelompok, masing-masing kelompok terdiri dari 18 ekor. Kelompok kontrol diberikan nasi putih 3,11 gr dan aquabides sedangkan kelompok perlakuan diberikan Nutrisure Gold® 0,93 gr 3 kali sehari selama 28 hari. Hasil penelitian menunjukkan rerata kadar estrogen kelompok kontrol 44,26±11,49 pg/ml dan rerata kelompok perlakuan 45,30±7,66 pg/ml. Rerata kadar progesteron kelompok kontrol 22,43±3,62 ng/ml dan rerata kelompok perlakuan 27,44±2,28 ng/ml. Analisis kemaknaan kadar estrogen dengan uji t-independent menunjukkan t = -0.320 dan p = 0,751 (P >0,05). Analisis kemaknaan kadar progesteron dengan uji t independent menunjukkan nilai t = -4,978 dan p = 0.000 (P < 0,05). Simpulan: Setelah diberi perlakuan, rerata kadar estrogen pada kedua kelompok tidak berbeda bermakna tetapi rerata kadar progesteron pada kedua kelompok menunjukkan perbedaan bermakna.Kata kunci: makanan pengganti cair, estrogen, progesteron

scholarly journals Nigella sativa EXTRACT IMPROVES SEMINIFEROUS TUBULE EPITHELIAL THICKNESS IN LEAD ACETATE-EXPOSED BALB/C MICE

2017 ◽  
Vol 53 (3) ◽  
pp. 180
Author(s):  
Alis Nur Diana ◽  
Reny I’tishom ◽  
Sri Agus Sudjarwo
Keyword(s):  
Treatment Group ◽  
Mus Musculus ◽  
Group Treatment ◽  
Lead Acetate ◽  
Nigella Sativa ◽  
The Body ◽  
Control Group ◽  
Tubular Epithelium ◽  
Control Group Design ◽  
Black Cumin

Lead that enters the body may lead to increased production of ROS (Reactive Oxygen Species) that may affect reproductive system. Black cumin (Nigella sativa) extract contains high antioxidant, tymoquinone, that may be used to suppress oxidative stress induced by lead in animal experiments. This study aimed to prove that black cumin (Nigella sativa) extract improves the thickness of seminiferous tubular epithelium in Balb/c mice exposed to lead (Pb) acetate. This study used post-test only control group design. The subjects in this study were 30 Balb/c male mice (Mus musculus), divided into 5 groups. K-group: control group without lead acetate and black cumin extract, K+ group: group with 50 mg/kgBW of lead acetate for 28 days, P1 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.3 mg/gBW of black cumin extract for 21 days, P2 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.6 mg/gBW of black cumin extract for 21 days, and P3 group: treatment group with 50 mg/gBW of lead acetate for 28 days + 1.2 mg/gBW black cumin extract for 21 days. The results showed that the mean ± standard deviation of the highest thickness of seminiferous tubular epithelium was 45.80 ± 2.73 in the group that was exposed to acetate + 0.6 mg/gBW of black cumin (P2), and the lowest was 32.75 ± 4.07 in the group that was exposed to lead acetate (K+). The results were then analyzed by Anova test. The results showed that there was significant differences in the thickness of seminiferous tubular epithelium between P1, P2, P3 and K+. In conclusion, black cumin extract administration was proved to improve the thickness of seminiferous tubular epithelium in lead (Pb) acetate-exposed Balb/c mice (Mus musculus).

scholarly journals Pengaruh pendidikan gizi dalam upaya meningkatkan kepatuhan konsumsi zat besi melalui kelas ibu hamil

2015 ◽  
Vol 12 (2) ◽  
pp. 79
Author(s):  
Siti Sulastijah ◽  
Sumarni DW ◽  
Siti Helmyati
Keyword(s):  
Treatment Group ◽  
Pregnant Women ◽  
Nutrition Education ◽  
Group Treatment ◽  
Iron Supplementation ◽  
Daily Basis ◽  
T Test ◽  
Deficiency Anemia ◽  
Control Group ◽  
Control Group Design

Background: Iron deficiency anemia contributed to a number of 50% in anemia and caused mortality of 841,000 every year in the world. Nutrition education before iron supplementation is needed because of low compliance in pregnant women. Gatak Sub District that is located in Sukoharjo Regency had the highest risk in pregnant women by 20.02%.Objective: This research aimed to know the effect of nutrition education in an effort to increase compliance of  iron consumption through prenatal classes in Gatak Sub district Sukoharjo Regency.Method: The method of this research was quasi experiment with pre test-post test with non equivalent control group design. The samples of this research were 70 pregnant women who fulfilled inclusion and exclusion criteria. A number of 33 pregnant women were in treatment group and 37 pregnant women were in control group. Treatment group received a nutrition education through anemia booklet and iron supplementation which contained 60 mg sulfas ferosus and 400 µg folic acid on a daily basis in prenatal classes for 3 months. Control group did not receive a nutrition education, but still received a daily iron supplementation for 3 months. Independent t-test and paired t-test were used to analyze data.Results: The knowledge level (higher 12.59), compliance of iron consumption (15.16%), food intakes including energy (higher 9.24%), protein (higher 10.98%), and Fe (higher 6.68%), although those food intakes were still below the RDA, and hemoglobin level (higher 0.44 g/dl), in treatment group compared with control (p<0.05). The factors of age, education level, household expenditure, parity, ANC, nutritional status, and last birth spacing did not affect the compliance of iron consumption (p>0.05).Conclusion: The nutrition education affected the increase in compliance of iron consumption through prenatal classes in Gatak Sub District Sukoharjo Regency.

scholarly journals SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT ON MALE WHITE RATS KIDNEY NECROSIS

2017 ◽  
Vol 52 (1) ◽  
pp. 62
Author(s):  
Ardy Hamonangan Naibaho ◽  
Arifa Mustika ◽  
Anny Setijo Rahaju
Keyword(s):  
Treatment Group ◽  
Ethanol Extract ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Post Test ◽  
Experimental Laboratory ◽  
Group 2 ◽  
High Doses

Singawalang leaf is used as a traditional medicine by the communities suffering from pneumonia due to TB disease. However, experiments to explain it’s toxicity are few. Therefore, this study was conducted to disclose it’s toxicity. This was an experimental laboratory study using post–test only control group design. The sampling method was simple random sampling. The animals were randomly divided into 4 (four) groups, each group consisted of 8 (eight) mice, the control group (P0) received distilled water as a solvent material. Treatment group 1 (P1) obtained ethanol extract of singawalang (Petiveria alliaceae) in low doses of 90 mg/kgbw. Treatment group 2 (P2) obtained ethanol extract of singawalang (Petiveria alliaceae) in medium doses of 180 mg/kgbw. Treatment group 3 (P3) obtained ethanol extract of singawalang (Petiveria alliaceae) in high doses of 360 mg/kgbw. Th extracts were given with sonde once a day for 30 days. Results showed that the leaf ethanol extract singawalang (Petiveria alliaceae) does not induce sub-acute necrosis effects on kidney of male white mice (Mus musculus) in doses 90 mg/kgbw, 180 mg/kgbw, and 360 mg/kgbw.

scholarly journals Effects of per oral cypermethrin exposure on Bcl-2 expression in granulose cells and antral follicle count of Rattus norvegicus ovaries

2019 ◽  
Vol 26 (3) ◽  
pp. 123 ◽  
Author(s):  
Indrayanti Indrayanti ◽  
Bambang Rahardjo ◽  
Hidayat Sujuti
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Rattus Norvegicus ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Exposed Group ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Control Group Design ◽  
Antral Follicles

Objectives: To determine the effect of oral cypermethrin exposure on the decrease of Bcl-2 expression in granulosa cells and antral follicle count in Rattus norvegicus ovary.Materials and Methods: This was a true experimental study using post-test control group design. This study used 24 Rattus norvegicus which were divided into 4 groups, the control group, treatment group (P1) exposed to cypermethrin 10 mg/kg BW, treatment group (P2) exposed to cypermethrin 15 mg/kg BW and treatment group (P3) exposed to cypermethrin 20 mg/kg BW. The treatment groups were exposed to cypermethrin for 28 days. A surgery was conducted in proestrus phase and ovarian organs were taken. Bcl-2 expression examination was performed by using immunohistochemical method and the antral follicle count was assessed with the Hematoxillin Eosin (HE)Results: This study showed the decrease of Bcl-2 expression in cypermethrin-exposed group compared to control group. There was a decrease in antral follicles count in cypermethrin-exposed group compared to control group.Conclusion: Cypermethrin can decrease Bcl-2 expression and antral follicles count in Rattus norvegicus ovaries.

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